Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_J11
(625 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 223 2e-59
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 196 2e-51
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 97 1e-21
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.18
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 31 0.18
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 1.7
SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 2.2
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 2.2
SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|ch... 26 5.1
SPBC25H2.10c |||tRNA acetyltransferase |Schizosaccharomyces pomb... 25 6.7
SPAC23D3.02 |rfc2||DNA replication factor C complex subunit Rfc2... 25 6.7
SPAC27F1.08 |pdt1||Nramp family manganese ion transporter|Schizo... 25 6.7
SPAP8A3.03 |||ZIP zinc transporter 1|Schizosaccharomyces pombe|c... 25 8.9
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 223 bits (544), Expect = 2e-59
Identities = 112/156 (71%), Positives = 131/156 (83%)
Frame = +2
Query: 62 PIYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAI 241
P+Y PFFGVMG +AI+F+S GAAYGTAK+G GI+AM V+RP+ I+K+ IPVVMAGIIAI
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66
Query: 242 YGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVXFSGLAAGFAIGIVGDASVRGTAQQ 421
YGLVV+VLI+G+L Q +LY GFI LGAGL+V +GLAAGFAIGIVGDA VRGTAQQ
Sbjct: 67 YGLVVSVLISGNLKQI---LSLYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQ 123
Query: 422 PRLFVGMILILIFAEVLGLYGLIVAIYLYTKQ*TNL 529
PRLFV MILILIFAEVLGLYGLIVA+ L T+ N+
Sbjct: 124 PRLFVAMILILIFAEVLGLYGLIVALLLNTRATDNV 159
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 196 bits (479), Expect = 2e-51
Identities = 93/151 (61%), Positives = 118/151 (78%)
Frame = +2
Query: 62 PIYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAI 241
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE +MKS+IPVVM+GII +
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 242 YGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVXFSGLAAGFAIGIVGDASVRGTAQQ 421
YGLV++VLIAG + P N+Y+L+ GFIHL AGLAV +G+AAG+AIG+VGD V+ +Q
Sbjct: 67 YGLVMSVLIAGDMS-PDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQ 125
Query: 422 PRLFVGMILILIFAEVLGLYGLIVAIYLYTK 514
R+FV M+LILIFAEVLGLYGLIV + L TK
Sbjct: 126 DRIFVSMVLILIFAEVLGLYGLIVGLILQTK 156
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 97.5 bits (232), Expect = 1e-21
Identities = 50/147 (34%), Positives = 81/147 (55%), Gaps = 5/147 (3%)
Frame = +2
Query: 80 FGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAIYGLVVA 259
+G++G AS + F +GAA+G GT I AV P K++I ++ ++AIY L++A
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104
Query: 260 VLIAGSLDQ--PSNNYT---LYKGFIHLGAGLAVXFSGLAAGFAIGIVGDASVRGTAQQP 424
++ + ++ P+ YT Y GF G+ V L G +GI G ++ AQ
Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDA 164
Query: 425 RLFVGMILILIFAEVLGLYGLIVAIYL 505
LFV ++++ IF VLGL+GLIV + +
Sbjct: 165 SLFVKVLVVEIFGSVLGLFGLIVGLLI 191
Score = 41.9 bits (94), Expect = 7e-05
Identities = 21/63 (33%), Positives = 33/63 (52%)
Frame = +2
Query: 326 LGAGLAVXFSGLAAGFAIGIVGDASVRGTAQQPRLFVGMILILIFAEVLGLYGLIVAIYL 505
LG V F + A + I I G + + G + PR+ ++ +IF EV+ +Y LI+AI
Sbjct: 48 LGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIAIVF 107
Query: 506 YTK 514
K
Sbjct: 108 SAK 110
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.7 bits (66), Expect = 0.18
Identities = 19/57 (33%), Positives = 27/57 (47%)
Frame = -2
Query: 483 PYRPNTSAKMRIRIIPTNNLGCWAVPRTLASPTMPMAKPAARPEKXTARPAPK*MNP 313
P RP ++A ++ PT +VP ++P MP P+A P A AP NP
Sbjct: 1691 PVRPQSAAPPQMSA-PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 30.7 bits (66), Expect = 0.18
Identities = 21/69 (30%), Positives = 30/69 (43%)
Frame = +2
Query: 206 IIPVVMAGIIAIYGLVVAVLIAGSLDQPSNNYTLYKGFIHLGAGLAVXFSGLAAGFAIGI 385
II +AG+IA + +++ IAG + G ++ G L LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVI-------AAMVGILYFGHWLVYKILILAFGFKIVT 245
Query: 386 VGDASVRGT 412
GD V T
Sbjct: 246 SGDVCVSNT 254
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 27.5 bits (58), Expect = 1.7
Identities = 16/80 (20%), Positives = 37/80 (46%), Gaps = 2/80 (2%)
Frame = +2
Query: 77 FFG-VMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMR-PEQIMKSIIPVVMAGIIAIYGL 250
F+G ++G + F+ + + ++ G + I+ S+ P+V++ IIA +
Sbjct: 1439 FYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMSVAPLVLSAIIAFFFF 1498
Query: 251 VVAVLIAGSLDQPSNNYTLY 310
+ +++ L S Y +Y
Sbjct: 1499 CLGIMLRPILGDRSKTYGVY 1518
>SPAC17G8.12 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 608
Score = 27.1 bits (57), Expect = 2.2
Identities = 13/50 (26%), Positives = 24/50 (48%)
Frame = -2
Query: 486 SPYRPNTSAKMRIRIIPTNNLGCWAVPRTLASPTMPMAKPAARPEKXTAR 337
S Y+P++S+K+ + + G A+P+ P P +P R T +
Sbjct: 401 SSYKPSSSSKVSAKSVSRKPTGAPAIPK--LPPKHPSRQPTVRATPSTGK 448
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.1 bits (57), Expect = 2.2
Identities = 21/72 (29%), Positives = 33/72 (45%)
Frame = +2
Query: 65 IYGPFFGVMGAASAIIFSSLGAAYGTAKSGTGIAAMAVMRPEQIMKSIIPVVMAGIIAIY 244
+YGPF+ A+ FS+ Y +G G + ++ K +I A II Y
Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137
Query: 245 GLVVAVLIAGSL 280
++AVL+ G L
Sbjct: 138 TTIIAVLLWGIL 149
>SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 815
Score = 25.8 bits (54), Expect = 5.1
Identities = 11/29 (37%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
Frame = +1
Query: 508 HEAVNQPEHTQLQSPVLRI-LYDENLIQS 591
H +N P +T+LQS + + Y E L++S
Sbjct: 279 HHLLNNPSNTELQSLIKKYSFYSERLVKS 307
>SPBC25H2.10c |||tRNA acetyltransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 287
Score = 25.4 bits (53), Expect = 6.7
Identities = 13/45 (28%), Positives = 21/45 (46%)
Frame = +3
Query: 36 HIQEKWPKIQSMDLSLELWGRRLPSSSAPLVLHMAQPSQAPVLPP 170
HI WP+ QS D E G L + A + + + ++ +L P
Sbjct: 62 HIATLWPETQSSDQENEEEGEELEDAVAKEINSLQKKNKKELLTP 106
>SPAC23D3.02 |rfc2||DNA replication factor C complex subunit
Rfc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 25.4 bits (53), Expect = 6.7
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +2
Query: 506 YTKQ*TNLNTLNSSRRCSGSYTTRILSNLRSVTMRAQ 616
YTK +N+ TL+ G T ILS L V ++ +
Sbjct: 262 YTKNVSNIETLSRDVAAEGYSTGIILSQLHDVLLKEE 298
>SPAC27F1.08 |pdt1||Nramp family manganese ion
transporter|Schizosaccharomyces pombe|chr 1|||Manual
Length = 521
Score = 25.4 bits (53), Expect = 6.7
Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 4/41 (9%)
Frame = +2
Query: 299 YTLYKGFIHLGAG----LAVXFSGLAAGFAIGIVGDASVRG 409
Y L K ++ + G +A+ FSG++AG+ I G G
Sbjct: 359 YDLLKEYVSISCGRLFAVALLFSGMSAGYVCTIAGQIVSEG 399
>SPAP8A3.03 |||ZIP zinc transporter 1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 453
Score = 25.0 bits (52), Expect = 8.9
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +2
Query: 440 MILILIFAEVLGLYGLIVAIYLYT 511
++++ + V GL G IVA Y+YT
Sbjct: 358 VLVLQMITMVTGLLGAIVATYIYT 381
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,937,145
Number of Sequences: 5004
Number of extensions: 67261
Number of successful extensions: 194
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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