Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_I02
(525 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 187 3e-48
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 186 1e-47
At1g21730.1 68414.m02720 kinesin-related protein (MKRP1) Similar... 31 0.36
At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family... 30 1.1
At2g18470.1 68415.m02151 protein kinase family protein contains ... 29 1.4
At1g70370.1 68414.m08095 BURP domain-containing protein / polyga... 29 1.4
At4g08380.1 68417.m01384 proline-rich extensin-like family prote... 29 1.9
At2g14660.1 68415.m01649 expressed protein contains Pfam PF04543... 29 2.5
At5g56250.2 68418.m07020 expressed protein 27 5.8
At5g56250.1 68418.m07019 expressed protein 27 5.8
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 27 5.8
At4g29330.1 68417.m04191 Der1-like family protein / degradation ... 27 5.8
At4g04040.1 68417.m00574 pyrophosphate--fructose-6-phosphate 1-p... 27 5.8
At1g74800.1 68414.m08666 galactosyltransferase family protein co... 27 5.8
At5g09350.1 68418.m01083 phosphatidylinositol 4-kinase, putative... 27 7.7
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 27 7.7
At3g30230.1 68416.m03820 myosin heavy chain-related similar to M... 27 7.7
At2g05520.1 68415.m00584 glycine-rich protein (GRP) identical to... 27 7.7
At1g76010.1 68414.m08825 expressed protein 27 7.7
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 187 bits (456), Expect = 3e-48
Identities = 87/157 (55%), Positives = 105/157 (66%), Gaps = 2/157 (1%)
Frame = +2
Query: 59 MSLSVARPLVSVYSEKSEVVAGAS--LPLPFVFKAPIRPDLVNDVHVSMSKNARQPYCVS 232
M+ + ARPLV++ + ++ S + LP V AP+RPD+VN VH +S N+RQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 233 KEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXX 412
K+AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 61 KKAGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRV 120
Query: 413 XXXXXXXXXXXXXXXXXXXXXXQARGHIVEKIPELPL 523
ARGH +E +PE+PL
Sbjct: 121 NVNMKRHAIVSAIAATAVPALVMARGHKIENVPEMPL 157
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 186 bits (452), Expect = 1e-47
Identities = 87/154 (56%), Positives = 102/154 (66%), Gaps = 2/154 (1%)
Frame = +2
Query: 68 SVARPLVSVYSEKSEVVAGAS--LPLPFVFKAPIRPDLVNDVHVSMSKNARQPYCVSKEA 241
+ ARPLV+V ++ S + LP V AP+RPD+VN VH +S N+RQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 242 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXX 421
GHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 65 GHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVN 124
Query: 422 XXXXXXXXXXXXXXXXXXXQARGHIVEKIPELPL 523
ARGH +E +PE+PL
Sbjct: 125 MKRHAIVSAIAATAVPALVMARGHKIENVPEMPL 158
>At1g21730.1 68414.m02720 kinesin-related protein (MKRP1) Similar to
gb|U06698 neuronal kinesin heavy chain from Homo sapiens
and contains a PF|00225 Kinesin motor domain. EST
gb|AA042507 comes from this gene; identical to cDNA
MKRP1 mRNA for kinesin-related protein, GI:16902291,
kinesin-related protein [Arabidopsis thaliana]
GI:16902292
Length = 890
Score = 31.5 bits (68), Expect = 0.36
Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = -1
Query: 189 WTSLTRS-GLMGALNTNGRGSEAPATTSLFSEYTDTK 82
W L +S G+ G L T+G S+A + S S+YT TK
Sbjct: 549 WLKLKKSDGVAGTLPTDGNQSQASGSPSSSSKYTQTK 585
>At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 340
Score = 29.9 bits (64), Expect = 1.1
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = -1
Query: 402 CQRLHGLVGANIRPPRHMLPNAPCPDLWVPPPRTRGIRATARPVPQDSA 256
CQ+ G V + P H +P L PPP++ + + P P S+
Sbjct: 169 CQQSLGFVAPMMAQPMHQIPTTIVLSLPPPPPQSLSLSLPSPPQPPPSS 217
>At2g18470.1 68415.m02151 protein kinase family protein contains
Pfam PF00069: Protein kinase domain
Length = 633
Score = 29.5 bits (63), Expect = 1.4
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = -3
Query: 508 NLLNNMPSSLNEGRYPGRCHGGGHGSTSLP*VHATVPAPP 389
N NN PS ++G R +GG +G P + P+PP
Sbjct: 66 NSSNNSPSPPSQGGGGERGNGGNNGGNDTPPSRGSPPSPP 105
>At1g70370.1 68414.m08095 BURP domain-containing protein /
polygalacturonase, putative similar to polygalacturonase
isoenzyme 1 beta subunit [Lycopersicon esculentum]
GI:170480; contains Pfam profile PF03181: BURP domain
Length = 626
Score = 29.5 bits (63), Expect = 1.4
Identities = 18/57 (31%), Positives = 25/57 (43%)
Frame = -1
Query: 195 DTWTSLTRSGLMGALNTNGRGSEAPATTSLFSEYTDTKGRATDRLISLYQRSYSSKK 25
DT+TS G + N N G+ A F+ Y D K D S Y + +S+K
Sbjct: 278 DTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRD-KANVGDDSFSSYAKDSNSEK 333
>At4g08380.1 68417.m01384 proline-rich extensin-like family protein
contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 437
Score = 29.1 bits (62), Expect = 1.9
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -1
Query: 363 PPRHMLPNAPCPDLWVPPP 307
PP P PCPD++ PPP
Sbjct: 385 PPYAYSPPPPCPDVYKPPP 403
>At2g14660.1 68415.m01649 expressed protein contains Pfam PF04543:
Family of unknown function (DUF589); similar to cThy28kD
(GI:995778) [Gallus gallus]; similar to thymocyte
protein mThy28 (GI:23978374) [Mus musculus]
Length = 158
Score = 28.7 bits (61), Expect = 2.5
Identities = 14/38 (36%), Positives = 19/38 (50%)
Frame = -3
Query: 223 VGLARVLRH*YVDIIDEVRSDGRLEYKREGERSPCDDL 110
VG+ V R Y D + V +G ++ K GE C DL
Sbjct: 65 VGVVEVSREWYTDDAEGVEGEGAVDVKAIGEMRKCVDL 102
>At5g56250.2 68418.m07020 expressed protein
Length = 811
Score = 27.5 bits (58), Expect = 5.8
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -2
Query: 149 IQTGGGAKPLRRPHSSPNTPTLKVGLLTDSFRFISE 42
+ G +P+R+P+ P LKV +TD + E
Sbjct: 200 LSVDSGQQPIRKPNRPKTKPRLKVKTMTDIYASAKE 235
>At5g56250.1 68418.m07019 expressed protein
Length = 811
Score = 27.5 bits (58), Expect = 5.8
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -2
Query: 149 IQTGGGAKPLRRPHSSPNTPTLKVGLLTDSFRFISE 42
+ G +P+R+P+ P LKV +TD + E
Sbjct: 200 LSVDSGQQPIRKPNRPKTKPRLKVKTMTDIYASAKE 235
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 27.5 bits (58), Expect = 5.8
Identities = 15/58 (25%), Positives = 25/58 (43%), Gaps = 3/58 (5%)
Frame = +2
Query: 227 VSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAPTKPW 391
+ + G + +AE + + + ++P G G G+ G FGN GG F W
Sbjct: 81 IRRRMGAKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGDISKW 138
>At4g29330.1 68417.m04191 Der1-like family protein / degradation in
the ER-like family protein contains Pfam profile:
PF04511 Der1-like family
Length = 266
Score = 27.5 bits (58), Expect = 5.8
Identities = 27/109 (24%), Positives = 41/109 (37%), Gaps = 7/109 (6%)
Frame = +2
Query: 83 LVSVYSEKSEVVAGASLPLPFVFKAPIRPDLVNDV--HVSMSKNARQPYCVSKE-----A 241
L + + K+ + A L L +F +PI PDL+ + H+ P K
Sbjct: 143 LYGLVTLKAFYLPWAMLALDVIFGSPIMPDLLGIIAGHLYYFLTVLHPLATGKNYLKTPK 202
Query: 242 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKP 388
W G VA + + GG +G GA G + GG + P
Sbjct: 203 WVNKIVARWRIGAPVASVR--QAGGVGAAGPGAGGGVGGGGAYSSARAP 249
>At4g04040.1 68417.m00574 pyrophosphate--fructose-6-phosphate
1-phosphotransferase beta subunit, putative /
pyrophosphate-dependent 6-phosphofructose-1-kinase,
putative strong similarity to SP|Q41141
Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
beta subunit (EC 2.7.1.90) (PFP) (6-phosphofructokinase,
pyrophosphate-dependent) (Pyrophosphate-dependent
6-phosphofructose-1-kinase) (PPI-PFK) {Ricinus communis}
Length = 585
Score = 27.5 bits (58), Expect = 5.8
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = +2
Query: 83 LVSVYSEKSEVVAGASLPLPFVFKAPIR 166
+ SVYSE +LPLP VFK P +
Sbjct: 26 VTSVYSEVQASRIDHTLPLPSVFKTPFK 53
>At1g74800.1 68414.m08666 galactosyltransferase family protein
contains Pfam profile: PF01762 galactosyltransferase
Length = 672
Score = 27.5 bits (58), Expect = 5.8
Identities = 14/49 (28%), Positives = 21/49 (42%), Gaps = 2/49 (4%)
Frame = -2
Query: 383 WSARTYVRRGTCYRMH--PAQICXXXXXXXXXXGLQHDRYPKTRRWFDD 243
WS + + + +CYRM PAQ C + D + K +W D
Sbjct: 257 WSKKPVIEQNSCYRMQWGPAQRCEGWKSRDDEETV--DSHVKCEKWIRD 303
>At5g09350.1 68418.m01083 phosphatidylinositol 4-kinase, putative
strong similarity to gi:4467359
Length = 1116
Score = 27.1 bits (57), Expect = 7.7
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -2
Query: 137 GGAKPLRRPHSSPNTPTLKV 78
G PL RPH+ P+TP KV
Sbjct: 148 GEWSPLMRPHNEPSTPGSKV 167
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 27.1 bits (57), Expect = 7.7
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +2
Query: 134 PLPFVFKAPIRPDLVNDVHVSMSKNARQPYCV 229
P+PF K+P +PD++ H + N+R +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
>At3g30230.1 68416.m03820 myosin heavy chain-related similar to
Myosin heavy chain, non-muscle (Zipper protein) (Myosin
II)(SP:Q99323) {Drosophila melanogaster}
Length = 527
Score = 27.1 bits (57), Expect = 7.7
Identities = 14/37 (37%), Positives = 18/37 (48%)
Frame = -1
Query: 363 PPRHMLPNAPCPDLWVPPPRTRGIRATARPVPQDSAL 253
PPR M P P P + PPPR G + P+ A+
Sbjct: 225 PPRSMGP--PLPVVTSPPPRLSGEKTDRGVTPRQEAM 259
>At2g05520.1 68415.m00584 glycine-rich protein (GRP) identical to
glycine-rich protein; atGRP (GI:259447) [Arabidopsis
thaliana]
Length = 145
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +2
Query: 269 GTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG 364
G GR R +GGG + G G+ G+ CR G
Sbjct: 88 GGGRYQGGGGRYQGGGGRQGGGGSGGSYCRHG 119
>At1g76010.1 68414.m08825 expressed protein
Length = 350
Score = 27.1 bits (57), Expect = 7.7
Identities = 17/44 (38%), Positives = 19/44 (43%)
Frame = +2
Query: 230 SKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG 361
S+ G + G GR R R RGGG R G G F N G
Sbjct: 302 SQGRGGYDGPQGRGRGRGRGRGGRGRGGG--RGGDGGFNNRSDG 343
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,854,417
Number of Sequences: 28952
Number of extensions: 222337
Number of successful extensions: 799
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 753
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 796
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 967280384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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