BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I09A02NGRL0002_H22 (577 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family prote... 167 5e-42 At3g59630.1 68416.m06653 diphthamide synthesis DPH2 family prote... 59 2e-09 At5g20790.1 68418.m02470 expressed protein predicted protein, Ar... 29 2.2 At4g21250.1 68417.m03072 expressed protein contains Pfam profi... 27 6.8 At3g62820.1 68416.m07058 invertase/pectin methylesterase inhibit... 27 6.8 At2g30350.2 68415.m03694 endo/excinuclease amino terminal domain... 27 9.0 At2g30350.1 68415.m03693 endo/excinuclease amino terminal domain... 27 9.0 At2g16440.1 68415.m01883 DNA replication licensing factor, putat... 27 9.0 At1g65370.1 68414.m07416 meprin and TRAF homology domain-contain... 27 9.0 >At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family protein contains InterPro accession IPR002728: Diphthamide synthesis DPH2 protein Length = 453 Score = 167 bits (406), Expect = 5e-42 Identities = 77/133 (57%), Positives = 97/133 (72%), Gaps = 1/133 (0%) Frame = +2 Query: 182 NKIPDELLNDPLLNRACEGLPQNYNFEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCD 361 N+IPD +L D LN A LP Y+FE+HK +WRI+T AKR+ALQ+PEGL M+A TL D Sbjct: 24 NQIPDSILGDASLNAAISILPSIYHFEVHKCVWRIKTSNAKRIALQLPEGLLMYALTLSD 83 Query: 362 IVETFTDAD-TVIMGDVTYGACCVDDFTAVALGVDLLVHYGHSCLIPIDQTNTIKVLYIF 538 I +F A ++GDVTYGACCVDDF+A ALG DLL+HYGHSCL+PID T I LY+F Sbjct: 84 IFTSFAGASHCFVLGDVTYGACCVDDFSACALGADLLIHYGHSCLVPIDSTK-IPCLYVF 142 Query: 539 VDFKIDPAHFVDT 577 V+ +ID ++T Sbjct: 143 VEIQIDVKCLLNT 155 >At3g59630.1 68416.m06653 diphthamide synthesis DPH2 family protein contains InterPro accession IPR002728: Diphthamide synthesis DPH2 protein Length = 491 Score = 58.8 bits (136), Expect = 2e-09 Identities = 31/100 (31%), Positives = 56/100 (56%), Gaps = 6/100 (6%) Frame = +2 Query: 257 FEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCDIVET----FTDADT--VIMGDVTYG 418 +EI++T I + R+ALQ P+ L +T + +++ TD + +M D TYG Sbjct: 9 YEINRTAEFIISKSFTRIALQFPDELLKDSTKVVSALKSKTRLLTDREVRFFVMADTTYG 68 Query: 419 ACCVDDFTAVALGVDLLVHYGHSCLIPIDQTNTIKVLYIF 538 +CC+D+ A+ + + +VHYG +CL P T+ + ++F Sbjct: 69 SCCIDEVGALHIDSECVVHYGQTCLSP---TSVLPAFFVF 105 >At5g20790.1 68418.m02470 expressed protein predicted protein, Arabidopsis thaliana Length = 193 Score = 29.1 bits (62), Expect = 2.2 Identities = 17/73 (23%), Positives = 32/73 (43%) Frame = +2 Query: 194 DELLNDPLLNRACEGLPQNYNFEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCDIVET 373 +EL N + P++ + +T+ R++ K L +G+T T+C V+ Sbjct: 60 EELTGSKSENESGRLEPKSITGPVKETVARVKETVTKTEPLICDDGVTKGKLTMCYEVDV 119 Query: 374 FTDADTVIMGDVT 412 D + GD+T Sbjct: 120 DVDGGRCVNGDLT 132 >At4g21250.1 68417.m03072 expressed protein contains Pfam profile: PF01925 domain of unknown function DUF81 Length = 449 Score = 27.5 bits (58), Expect = 6.8 Identities = 24/83 (28%), Positives = 38/83 (45%), Gaps = 1/83 (1%) Frame = +2 Query: 221 NRACEGLPQNYNFEIHKT-IWRIRTLKAKRVALQMPEGLTMFATTLCDIVETFTDADTVI 397 N+ E LPQ+++ +HK WR ++ L++ + M A LC + + A Sbjct: 20 NQEEENLPQSHHNLLHKVQQWRTSLKESSAAELKLSSAIIM-AGVLCFLAALISSA---- 74 Query: 398 MGDVTYGACCVDDFTAVALGVDL 466 G + G + T VA GVDL Sbjct: 75 -GGIGGGGLFIPIMTIVA-GVDL 95 >At3g62820.1 68416.m07058 invertase/pectin methylesterase inhibitor family protein low similarity to pectinesterase from Arabidopsis thaliana SP|Q43867, Lycopersicon esculentum SP|Q43143; contains Pfam profile PF04043: Plant invertase/pectin methylesterase inhibitor Length = 194 Score = 27.5 bits (58), Expect = 6.8 Identities = 14/44 (31%), Positives = 24/44 (54%) Frame = +2 Query: 230 CEGLPQNYNFEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCD 361 C L Q+ +HK++ +RTL+A QM + T +++L D Sbjct: 103 CRELLQDSLDSLHKSLAVLRTLRASEFQQQMSDLATWLSSSLTD 146 >At2g30350.2 68415.m03694 endo/excinuclease amino terminal domain-containing protein contains Pfam domain PF01541: Endo/excinuclease amino terminal domain Length = 368 Score = 27.1 bits (57), Expect = 9.0 Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 1/38 (2%) Frame = +2 Query: 167 NIRAVNKIPDELLNDPLLNRACEGLPQNYNFEI-HKTI 277 +IRA K P+ + ND L + C GL + E+ H TI Sbjct: 267 SIRATEKEPETVFNDRLASFTCFGLVEIVEDEVSHGTI 304 >At2g30350.1 68415.m03693 endo/excinuclease amino terminal domain-containing protein contains Pfam domain PF01541: Endo/excinuclease amino terminal domain Length = 334 Score = 27.1 bits (57), Expect = 9.0 Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 1/38 (2%) Frame = +2 Query: 167 NIRAVNKIPDELLNDPLLNRACEGLPQNYNFEI-HKTI 277 +IRA K P+ + ND L + C GL + E+ H TI Sbjct: 233 SIRATEKEPETVFNDRLASFTCFGLVEIVEDEVSHGTI 270 >At2g16440.1 68415.m01883 DNA replication licensing factor, putative similar to SP|P49717 DNA replication licensing factor MCM4 (CDC21 homolog) {Mus musculus}, SP|P29458 Cdc21 protein {Schizosaccharomyces pombe}; contains Pfam profile PF00493: MCM2/3/5 family Length = 847 Score = 27.1 bits (57), Expect = 9.0 Identities = 12/49 (24%), Positives = 25/49 (51%) Frame = +2 Query: 329 GLTMFATTLCDIVETFTDADTVIMGDVTYGACCVDDFTAVALGVDLLVH 475 GLT + + ET ++ +++ D G CC+D+F ++ ++H Sbjct: 517 GLTAYVAKDPETGETVLESGALVLSD--RGICCIDEFDKMSDSARSMLH 563 >At1g65370.1 68414.m07416 meprin and TRAF homology domain-containing protein / MATH domain-containing protein contains Pfam profile PF00917: MATH domain Length = 227 Score = 27.1 bits (57), Expect = 9.0 Identities = 14/43 (32%), Positives = 23/43 (53%) Frame = +2 Query: 266 HKTIWRIRTLKAKRVALQMPEGLTMFATTLCDIVETFTDADTV 394 HKT+W + + A A +P+ L +L D+ E + D DT+ Sbjct: 175 HKTVWLQQWIMAATKARGIPQSL-----SLADLQEAYLDEDTL 212 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,340,960 Number of Sequences: 28952 Number of extensions: 261197 Number of successful extensions: 648 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 629 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 646 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1121903184 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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