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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0002_H22
         (577 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family prote...   167   5e-42
At3g59630.1 68416.m06653 diphthamide synthesis DPH2 family prote...    59   2e-09
At5g20790.1 68418.m02470 expressed protein predicted protein, Ar...    29   2.2  
At4g21250.1 68417.m03072 expressed protein   contains Pfam profi...    27   6.8  
At3g62820.1 68416.m07058 invertase/pectin methylesterase inhibit...    27   6.8  
At2g30350.2 68415.m03694 endo/excinuclease amino terminal domain...    27   9.0  
At2g30350.1 68415.m03693 endo/excinuclease amino terminal domain...    27   9.0  
At2g16440.1 68415.m01883 DNA replication licensing factor, putat...    27   9.0  
At1g65370.1 68414.m07416 meprin and TRAF homology domain-contain...    27   9.0  

>At5g62030.1 68418.m07786 diphthamide synthesis DPH2 family protein 
           contains InterPro accession IPR002728: Diphthamide
           synthesis DPH2 protein
          Length = 453

 Score =  167 bits (406), Expect = 5e-42
 Identities = 77/133 (57%), Positives = 97/133 (72%), Gaps = 1/133 (0%)
 Frame = +2

Query: 182 NKIPDELLNDPLLNRACEGLPQNYNFEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCD 361
           N+IPD +L D  LN A   LP  Y+FE+HK +WRI+T  AKR+ALQ+PEGL M+A TL D
Sbjct: 24  NQIPDSILGDASLNAAISILPSIYHFEVHKCVWRIKTSNAKRIALQLPEGLLMYALTLSD 83

Query: 362 IVETFTDAD-TVIMGDVTYGACCVDDFTAVALGVDLLVHYGHSCLIPIDQTNTIKVLYIF 538
           I  +F  A    ++GDVTYGACCVDDF+A ALG DLL+HYGHSCL+PID T  I  LY+F
Sbjct: 84  IFTSFAGASHCFVLGDVTYGACCVDDFSACALGADLLIHYGHSCLVPIDSTK-IPCLYVF 142

Query: 539 VDFKIDPAHFVDT 577
           V+ +ID    ++T
Sbjct: 143 VEIQIDVKCLLNT 155


>At3g59630.1 68416.m06653 diphthamide synthesis DPH2 family protein 
           contains InterPro accession IPR002728: Diphthamide
           synthesis DPH2 protein
          Length = 491

 Score = 58.8 bits (136), Expect = 2e-09
 Identities = 31/100 (31%), Positives = 56/100 (56%), Gaps = 6/100 (6%)
 Frame = +2

Query: 257 FEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCDIVET----FTDADT--VIMGDVTYG 418
           +EI++T   I +    R+ALQ P+ L   +T +   +++     TD +    +M D TYG
Sbjct: 9   YEINRTAEFIISKSFTRIALQFPDELLKDSTKVVSALKSKTRLLTDREVRFFVMADTTYG 68

Query: 419 ACCVDDFTAVALGVDLLVHYGHSCLIPIDQTNTIKVLYIF 538
           +CC+D+  A+ +  + +VHYG +CL P   T+ +   ++F
Sbjct: 69  SCCIDEVGALHIDSECVVHYGQTCLSP---TSVLPAFFVF 105


>At5g20790.1 68418.m02470 expressed protein predicted protein,
           Arabidopsis thaliana
          Length = 193

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 17/73 (23%), Positives = 32/73 (43%)
 Frame = +2

Query: 194 DELLNDPLLNRACEGLPQNYNFEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCDIVET 373
           +EL      N +    P++    + +T+ R++    K   L   +G+T    T+C  V+ 
Sbjct: 60  EELTGSKSENESGRLEPKSITGPVKETVARVKETVTKTEPLICDDGVTKGKLTMCYEVDV 119

Query: 374 FTDADTVIMGDVT 412
             D    + GD+T
Sbjct: 120 DVDGGRCVNGDLT 132


>At4g21250.1 68417.m03072 expressed protein   contains Pfam profile:
           PF01925 domain of unknown function DUF81
          Length = 449

 Score = 27.5 bits (58), Expect = 6.8
 Identities = 24/83 (28%), Positives = 38/83 (45%), Gaps = 1/83 (1%)
 Frame = +2

Query: 221 NRACEGLPQNYNFEIHKT-IWRIRTLKAKRVALQMPEGLTMFATTLCDIVETFTDADTVI 397
           N+  E LPQ+++  +HK   WR    ++    L++   + M A  LC +    + A    
Sbjct: 20  NQEEENLPQSHHNLLHKVQQWRTSLKESSAAELKLSSAIIM-AGVLCFLAALISSA---- 74

Query: 398 MGDVTYGACCVDDFTAVALGVDL 466
            G +  G   +   T VA GVDL
Sbjct: 75  -GGIGGGGLFIPIMTIVA-GVDL 95


>At3g62820.1 68416.m07058 invertase/pectin methylesterase inhibitor
           family protein low similarity to pectinesterase from
           Arabidopsis thaliana SP|Q43867, Lycopersicon esculentum
           SP|Q43143; contains Pfam profile PF04043: Plant
           invertase/pectin methylesterase inhibitor
          Length = 194

 Score = 27.5 bits (58), Expect = 6.8
 Identities = 14/44 (31%), Positives = 24/44 (54%)
 Frame = +2

Query: 230 CEGLPQNYNFEIHKTIWRIRTLKAKRVALQMPEGLTMFATTLCD 361
           C  L Q+    +HK++  +RTL+A     QM +  T  +++L D
Sbjct: 103 CRELLQDSLDSLHKSLAVLRTLRASEFQQQMSDLATWLSSSLTD 146


>At2g30350.2 68415.m03694 endo/excinuclease amino terminal
           domain-containing protein contains Pfam domain PF01541:
           Endo/excinuclease amino terminal domain
          Length = 368

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
 Frame = +2

Query: 167 NIRAVNKIPDELLNDPLLNRACEGLPQNYNFEI-HKTI 277
           +IRA  K P+ + ND L +  C GL +    E+ H TI
Sbjct: 267 SIRATEKEPETVFNDRLASFTCFGLVEIVEDEVSHGTI 304


>At2g30350.1 68415.m03693 endo/excinuclease amino terminal
           domain-containing protein contains Pfam domain PF01541:
           Endo/excinuclease amino terminal domain
          Length = 334

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
 Frame = +2

Query: 167 NIRAVNKIPDELLNDPLLNRACEGLPQNYNFEI-HKTI 277
           +IRA  K P+ + ND L +  C GL +    E+ H TI
Sbjct: 233 SIRATEKEPETVFNDRLASFTCFGLVEIVEDEVSHGTI 270


>At2g16440.1 68415.m01883 DNA replication licensing factor, putative
           similar to SP|P49717 DNA replication licensing factor
           MCM4 (CDC21 homolog) {Mus musculus}, SP|P29458 Cdc21
           protein {Schizosaccharomyces pombe}; contains Pfam
           profile PF00493: MCM2/3/5 family
          Length = 847

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 12/49 (24%), Positives = 25/49 (51%)
 Frame = +2

Query: 329 GLTMFATTLCDIVETFTDADTVIMGDVTYGACCVDDFTAVALGVDLLVH 475
           GLT +     +  ET  ++  +++ D   G CC+D+F  ++     ++H
Sbjct: 517 GLTAYVAKDPETGETVLESGALVLSD--RGICCIDEFDKMSDSARSMLH 563


>At1g65370.1 68414.m07416 meprin and TRAF homology domain-containing
           protein / MATH domain-containing protein contains Pfam
           profile PF00917: MATH domain
          Length = 227

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 14/43 (32%), Positives = 23/43 (53%)
 Frame = +2

Query: 266 HKTIWRIRTLKAKRVALQMPEGLTMFATTLCDIVETFTDADTV 394
           HKT+W  + + A   A  +P+ L     +L D+ E + D DT+
Sbjct: 175 HKTVWLQQWIMAATKARGIPQSL-----SLADLQEAYLDEDTL 212


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,340,960
Number of Sequences: 28952
Number of extensions: 261197
Number of successful extensions: 648
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 629
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 646
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1121903184
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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