Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_H03
(559 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein L20|Sc... 191 5e-50
SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosom... 191 5e-50
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 26 4.3
SPBC8D2.05c |sfi1||spindle pole body protein Sfi1|Schizosaccharo... 25 5.7
SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|c... 25 7.5
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 25 7.5
SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces pomb... 25 10.0
SPAC24H6.06 |sld3|mug175|DNA replication pre-initiation complex ... 25 10.0
>SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein
L20|Schizosaccharomyces pombe|chr 1|||Manual
Length = 176
Score = 191 bits (466), Expect = 5e-50
Identities = 96/174 (55%), Positives = 120/174 (68%), Gaps = 4/174 (2%)
Frame = +3
Query: 24 LKEYEVIGRKLPSESEPKPPLYKMRIFSPDPIVAKSRFWYFLRQLKKFKKTTGEIVSIME 203
LKEY+V+GRK+P+E EP P L++MR+F+P+ VAKSR+WYFL+ + K KK TGEIV+I E
Sbjct: 3 LKEYQVVGRKVPTEHEPVPKLFRMRLFAPNESVAKSRYWYFLKMINKVKKATGEIVAINE 62
Query: 204 IPEKSPVKIKNFGIWLRYESRSGVHNMYREYRDLSVGGAVTQCYRDMGARHRARAHSIQI 383
I E P+K K FGIW+RY+SRSG HNMY+E+RD + GAV Y DM ARHRAR SI+I
Sbjct: 63 ISEPKPLKAKVFGIWIRYDSRSGTHNMYKEFRDTTRVGAVEAMYADMAARHRARFRSIRI 122
Query: 384 IK-VEVIKAAACRRPQVKQFHTSKIGFPLPKR---VHQYKRLNTFAYKRPSTYF 533
+K VEV K RR VKQ + FPLP R V FA RPST++
Sbjct: 123 LKVVEVEKKEDVRRNYVKQLLNPHLKFPLPHRRTGVVGLAGKKVFAPHRPSTFY 176
>SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosomal
protein L20a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 176
Score = 191 bits (466), Expect = 5e-50
Identities = 96/174 (55%), Positives = 120/174 (68%), Gaps = 4/174 (2%)
Frame = +3
Query: 24 LKEYEVIGRKLPSESEPKPPLYKMRIFSPDPIVAKSRFWYFLRQLKKFKKTTGEIVSIME 203
LKEY+V+GRK+P+E EP P L++MR+F+P+ VAKSR+WYFL+ + K KK TGEIV+I E
Sbjct: 3 LKEYQVVGRKVPTEHEPVPKLFRMRLFAPNESVAKSRYWYFLKMINKVKKATGEIVAINE 62
Query: 204 IPEKSPVKIKNFGIWLRYESRSGVHNMYREYRDLSVGGAVTQCYRDMGARHRARAHSIQI 383
I E P+K K FGIW+RY+SRSG HNMY+E+RD + GAV Y DM ARHRAR SI+I
Sbjct: 63 ISEPKPLKAKVFGIWIRYDSRSGTHNMYKEFRDTTRVGAVEAMYADMAARHRARFRSIRI 122
Query: 384 IK-VEVIKAAACRRPQVKQFHTSKIGFPLPKR---VHQYKRLNTFAYKRPSTYF 533
+K VEV K RR VKQ + FPLP R V FA RPST++
Sbjct: 123 LKVVEVEKKEDVRRNYVKQLLNPHLKFPLPHRRTGVVGLAGKKVFAPHRPSTFY 176
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1428
Score = 25.8 bits (54), Expect = 4.3
Identities = 11/39 (28%), Positives = 19/39 (48%)
Frame = +3
Query: 27 KEYEVIGRKLPSESEPKPPLYKMRIFSPDPIVAKSRFWY 143
+E V+ + E P +R +SP+ ++ S FWY
Sbjct: 470 QEILVLKSSIEKEKVEIVPFLDIRKYSPNEVLCMSPFWY 508
>SPBC8D2.05c |sfi1||spindle pole body protein
Sfi1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 840
Score = 25.4 bits (53), Expect = 5.7
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +3
Query: 111 DPIVAKSRFWYFLRQLKKFK 170
D ++ RFWY+L + K F+
Sbjct: 476 DRLIVTFRFWYYLMKAKSFQ 495
>SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 559
Score = 25.0 bits (52), Expect = 7.5
Identities = 7/24 (29%), Positives = 12/24 (50%)
Frame = +3
Query: 78 PPLYKMRIFSPDPIVAKSRFWYFL 149
PP + P + K +FWY++
Sbjct: 534 PPKSNAELVDPSTLSGKDKFWYYI 557
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 25.0 bits (52), Expect = 7.5
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +1
Query: 436 NSTPARSVSRYQNAFTNTSVSIPLHTRGRAR 528
NS+P +S+S+Y NA N SI T +
Sbjct: 148 NSSPFKSLSQYWNAAYNKKESISSSTNSNIK 178
>SPBC365.14c |||UDP-glucose 4-epimerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 24.6 bits (51), Expect = 10.0
Identities = 8/15 (53%), Positives = 10/15 (66%)
Frame = -1
Query: 169 LNFFNCLKKYQKRDF 125
+N C+KKY RDF
Sbjct: 111 INLIECMKKYNVRDF 125
>SPAC24H6.06 |sld3|mug175|DNA replication pre-initiation complex
subunit Sld3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 668
Score = 24.6 bits (51), Expect = 10.0
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = +3
Query: 174 TTGEIVSIMEIPEKSPVKIKNFGIWLRYESRSGVHNMYR 290
T+G +S P K++ I R S SGVH++ R
Sbjct: 458 TSGASISNTSSPHSEASISKDYEILKRRRSNSGVHSLTR 496
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,313,112
Number of Sequences: 5004
Number of extensions: 46520
Number of successful extensions: 156
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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