Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0002_G06
         (386 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g63300.1 68414.m07156 expressed protein similar to Intracellu...    29   1.1  
At4g04695.1 68417.m00689 calcium-dependent protein kinase, putat...    28   1.9  
At5g11720.1 68418.m01369 alpha-glucosidase 1 (AGLU1) identical t...    27   3.3  
At1g48970.1 68414.m05489 eukaryotic translation initiation facto...    27   4.4  
At2g23350.1 68415.m02788 polyadenylate-binding protein, putative...    26   7.6  

>At1g63300.1 68414.m07156 expressed protein similar to Intracellular
            protein transport protein USO1 (Swiss-Prot:P25386)
            [Saccharomyces cerevisiae]; similar to Myosin II heavy
            chain, non muscle (Swiss-Prot:P08799) [Dictyostelium
            discoideum]
          Length = 1029

 Score = 29.1 bits (62), Expect = 1.1
 Identities = 24/98 (24%), Positives = 42/98 (42%), Gaps = 1/98 (1%)
 Frame = +3

Query: 6    LKNQKIFDFRIDT-KTKSVVLKTKADLNIVGNIVIELGEKSKLFSGTYTAQATSVGAAKY 182
            L+ ++  + R+D  KTK  V++ +A L       IEL  K  L      + A  +   K 
Sbjct: 749  LQAEQAENLRVDLEKTKKSVMEAEASLQRENMKKIELESKISLMRKESESLAAELQVIKL 808

Query: 183  GYDLKMDSTGVLHFEVGPEIINCENINDIKVQLDSELE 296
              D K  +  +L  E+      C+++     + D E+E
Sbjct: 809  AKDEKETAISLLQTELETVRSQCDDLKHSLSENDLEME 846


>At4g04695.1 68417.m00689 calcium-dependent protein kinase, putative
           / CDPK, putative similar to calcium-dependent protein
           kinase [Lycopersicon esculentum]
           gi|19171502|emb|CAC87494; contains protein kinase
           domain, Pfam:PF00069; contains EF hand domain
           (calcium-binding EF-hand), Pfam:PF00036,
           INTERPRO:IPR002048
          Length = 484

 Score = 28.3 bits (60), Expect = 1.9
 Identities = 26/104 (25%), Positives = 46/104 (44%), Gaps = 9/104 (8%)
 Frame = +3

Query: 6   LKNQKIFDFRIDTKTKSVVLKTKADLNIVGNIVIELGEKSKLFSGTYTAQATSVGAAKYG 185
           L++ K  +F + +  K+  +K    ++   ++ IE GE  + F+G+    A  V   KYG
Sbjct: 154 LRDLKPENFLLSSTDKNATVKA---IDFGCSVFIEEGEVHRKFAGSAYYIAPEVLQGKYG 210

Query: 186 YDLKMDSTGVL---------HFEVGPEIINCENINDIKVQLDSE 290
            +  + S G++          F   PE      I   K+ +DSE
Sbjct: 211 KEADIWSAGIILYILLCGKPPFVTEPEAQMFSEIKSAKIDVDSE 254


>At5g11720.1 68418.m01369 alpha-glucosidase 1 (AGLU1) identical to
           alpha-glucosidase 1 [Arabidopsis thaliana] GI:2323344
          Length = 902

 Score = 27.5 bits (58), Expect = 3.3
 Identities = 18/51 (35%), Positives = 26/51 (50%)
 Frame = +3

Query: 72  KADLNIVGNIVIELGEKSKLFSGTYTAQATSVGAAKYGYDLKMDSTGVLHF 224
           +A ++I G     L   + + SG YTA  T   AAK+  DL     G+L+F
Sbjct: 527 QAVVDITGKRPFILSRSTFVSSGKYTAHWTGDNAAKW-EDLAYSIPGILNF 576


>At1g48970.1 68414.m05489 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           guanine nucleotide exchange factor, eIF-2B, delta
           subunit [Mus musculus] GI:529428; contains Pfam profile
           PF01008: Initiation factor 2 subunit family
          Length = 756

 Score = 27.1 bits (57), Expect = 4.4
 Identities = 18/38 (47%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
 Frame = +3

Query: 234 PEIINCENIN-DIKVQLDSELENALRRDPDAKANKPAF 344
           P I+ CE      +V LDS   N L  DPDA AN P+F
Sbjct: 655 PVIVCCEAYKFHERVLLDSICSNELG-DPDAVANIPSF 691


>At2g23350.1 68415.m02788 polyadenylate-binding protein, putative /
           PABP, putative
          Length = 662

 Score = 26.2 bits (55), Expect = 7.6
 Identities = 16/35 (45%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +3

Query: 153 QATSVGAAKY-GYDLKMDSTGVLHFEVGPEIINCE 254
           Q  S  AAK  G  L+MD T VLH    PE +N +
Sbjct: 588 QIESEHAAKVTGMLLEMDQTEVLHLLESPEALNAK 622


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,756,009
Number of Sequences: 28952
Number of extensions: 139729
Number of successful extensions: 325
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 321
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 325
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 547638520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)