Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_F20
(644 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 131 4e-31
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 127 5e-30
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 126 1e-29
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 125 3e-29
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 32 0.28
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim... 30 1.1
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 28 4.6
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 27 8.1
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 131 bits (316), Expect = 4e-31
Identities = 79/207 (38%), Positives = 105/207 (50%), Gaps = 11/207 (5%)
Frame = +1
Query: 55 ALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHI-- 228
A ++ + GL +L+++L RSY++GY S+ DI VF + K P + +V RW++HI
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDA 62
Query: 229 ---ASYTPAERK-TWSEGVSXXXXXXXXXXXXXXSNXXXXXXXX-----LFGSGXXXXXX 381
S AE EG S S LFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSSPITEEAVATPPAADSKDTAAEEEDDDDVDLFGE-ETEEEK 121
Query: 382 XXXXXXXXXLKAYADKKSKKPALIAKSSIILDVXPWDDETDMKEMENQVRTIEMDGLLWG 561
+KA KK KSS+++D+ PWDDETDMK++E VR+I+M+GL WG
Sbjct: 122 KAAEERAASVKASTKKKES-----GKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWG 176
Query: 562 ASKLVPVGYGINKLQIMCVIEDDKVSV 642
ASKLVPVGYGI KL IMC I DD VS+
Sbjct: 177 ASKLVPVGYGIKKLHIMCTIVDDLVSI 203
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 127 bits (307), Expect = 5e-30
Identities = 72/199 (36%), Positives = 99/199 (49%), Gaps = 3/199 (1%)
Frame = +1
Query: 52 MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHIA 231
+ D+ T +G+ + ++LA ++Y+SG S D+KV+ V P+ + P+ +WY +A
Sbjct: 3 VTFSDLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVA 62
Query: 232 SYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXX---LFGSGXXXXXXXXXXXXX 402
S + GV + LFG
Sbjct: 63 SQLAKSFPGKAVGVQFGGSAAAAPAVEAEAPAAAADDDDDMDLFGDETEEEKKAAEE--- 119
Query: 403 XXLKAYADKKSKKPALIAKSSIILDVXPWDDETDMKEMENQVRTIEMDGLLWGASKLVPV 582
+ A K +KKP KSS+++DV PWDDETDMK++E VR +EM GL WGASKLVPV
Sbjct: 120 ---REAAKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPV 176
Query: 583 GYGINKLQIMCVIEDDKVS 639
GYGI KL IM I DD VS
Sbjct: 177 GYGIKKLTIMFTIVDDLVS 195
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 126 bits (305), Expect = 1e-29
Identities = 74/207 (35%), Positives = 104/207 (50%), Gaps = 11/207 (5%)
Frame = +1
Query: 55 ALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSH--- 225
A ++ + GL +L+++L RSY++GY S+ DI VF + K P + + RWY+H
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDA 62
Query: 226 ---IASYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXX-----LFGSGXXXXXX 381
I+ + EG + S LFG
Sbjct: 63 LLRISGVSAEGSGVIVEGSAPITEEAVATPPAADSKDAAADEEDDDDVDLFGE-ETEEEK 121
Query: 382 XXXXXXXXXLKAYADKKSKKPALIAKSSIILDVXPWDDETDMKEMENQVRTIEMDGLLWG 561
+KA KK KSS+++D+ PWDDETDMK++E V++I+M+GL WG
Sbjct: 122 KAAEERAASVKASTKKKES-----GKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWG 176
Query: 562 ASKLVPVGYGINKLQIMCVIEDDKVSV 642
ASKLVPVGYGI KLQI+C I DD VS+
Sbjct: 177 ASKLVPVGYGIKKLQILCTIVDDLVSI 203
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 125 bits (301), Expect = 3e-29
Identities = 71/198 (35%), Positives = 99/198 (50%), Gaps = 1/198 (0%)
Frame = +1
Query: 52 MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHIA 231
+ D+ T +GL L ++LA ++Y+SG S D+KV+ V + P P+ +WY +A
Sbjct: 3 VTFSDLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVA 62
Query: 232 SYTPAERKTWSEGVSXXXXXXXXXXXXXXSNXXXXXXXXLFGSGXXXXXXXXXXXXXXXL 411
S+ ++GV +
Sbjct: 63 SHLAKSFPGKADGVRVGGGVAPPSEAHPHTEEPAADGDGDDDDDIDLFADETEDEKKAAE 122
Query: 412 KAYADKK-SKKPALIAKSSIILDVXPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGY 588
+ A KK +KK KSS++L+V PWDDETDMK++E VR+++M GL WGASKLVPVGY
Sbjct: 123 EREAAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVPVGY 182
Query: 589 GINKLQIMCVIEDDKVSV 642
GI KL IM I DD VSV
Sbjct: 183 GIKKLTIMMTIVDDLVSV 200
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 32.3 bits (70), Expect = 0.28
Identities = 22/57 (38%), Positives = 32/57 (56%), Gaps = 9/57 (15%)
Frame = +1
Query: 85 LNELNQYLAERSYV--SGYTPSQADIKVFEQV-GKVPAAS------LPHVLRWYSHI 228
L +LN LA +S + +G TPS AD+ VF + V S +PHV+RW ++I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At5g08415.1 68418.m00991 lipoic acid synthase family protein
similar to lipoic acid synthase from Arabidopsis
thaliana [gi:3928758], from Mus musculus [gi:14669826]
Pfam profile PF04055: radical SAM domain protein
Length = 394
Score = 30.3 bits (65), Expect = 1.1
Identities = 17/44 (38%), Positives = 26/44 (59%)
Frame = +1
Query: 430 KSKKPALIAKSSIILDVXPWDDETDMKEMENQVRTIEMDGLLWG 561
K KP +I K+SI+L + D+E +KE +R I++D L G
Sbjct: 287 KISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 28.3 bits (60), Expect = 4.6
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +1
Query: 85 LNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASL 198
+N + + +R V G TP+QAD++V VG + A +
Sbjct: 386 MNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEI 423
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 27.5 bits (58), Expect = 8.1
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +1
Query: 85 LNELNQYLAERSYVSGYTPSQADIKVFEQVGKV 183
L +L Y YVS PSQA + + EQ GK+
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGKI 526
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,539,747
Number of Sequences: 28952
Number of extensions: 226033
Number of successful extensions: 561
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 549
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 560
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1334473344
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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