Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_F13
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co... 129 2e-30
At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si... 128 3e-30
At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si... 117 8e-27
At2g07676.1 68415.m00907 expressed protein 33 0.23
At4g22260.1 68417.m03220 alternative oxidase, putative / immutan... 31 0.54
At3g46530.1 68416.m05051 disease resistance protein, RPP13-like ... 30 1.2
At3g47890.1 68416.m05222 ubiquitin carboxyl-terminal hydrolase-r... 29 3.8
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 28 5.0
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 28 5.0
At4g16510.1 68417.m02498 YbaK/prolyl-tRNA synthetase-related con... 28 6.6
At2g25730.1 68415.m03084 expressed protein 28 6.6
At4g21660.1 68417.m03138 proline-rich spliceosome-associated (PS... 27 8.8
>At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein
contains Pfam PF00728: Glycosyl hydrolase family 20,
catalytic domain; contains Pfam PF02838: Glycosyl
hydrolase family 20, domain 2; similar to
Beta-hexosaminidase beta chain precursor (EC 3.2.1.52)
(N-acetyl-beta- glucosaminidase)
(Beta-N-acetylhexosaminidase) (Hexosaminidase B)
(Swiss-Prot:P07686) [Homo sapiens]
Length = 535
Score = 129 bits (311), Expect = 2e-30
Identities = 73/173 (42%), Positives = 100/173 (57%), Gaps = 7/173 (4%)
Frame = +2
Query: 188 VQELHVELTAPCEKLPYFGMDESYNLXXXX-----XXXXXXXXIWGVIRGLETWSQL--F 346
+Q LHV +++ ++L Y G DESY L ++G + GL+T+SQL F
Sbjct: 102 LQGLHVIISSSTDELEY-GADESYKLVVPSPEKPSYAQLEAKSVYGALHGLQTFSQLCHF 160
Query: 347 YLTNDFRELRINSTDIYDYPRYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLHWHI 526
L E+ + +I D PR+++RGLL+DTSRHYL L I ID+M K+NVLHWHI
Sbjct: 161 NLKKKVIEILMTPWNIIDQPRFSYRGLLIDTSRHYLPLPVIKNVIDSMTYAKLNVLHWHI 220
Query: 527 VDDQSFPYHSEKFPELSRLGAYHPTLVYTKRDIEIVVKHAAERGVRVLTEVDV 685
VD QSFP +P+L GAY + YT D +V +A RG+ VL E+DV
Sbjct: 221 VDTQSFPLEIPSYPKLWN-GAYSSSQRYTFEDAAEIVNYARRRGIHVLAEIDV 272
>At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein
similar to beta-hexosaminidase precursor SP:P43077 from
[Candida albicans]
Length = 580
Score = 128 bits (309), Expect = 3e-30
Identities = 65/152 (42%), Positives = 89/152 (58%), Gaps = 3/152 (1%)
Frame = +2
Query: 236 YFGMDESYNLXX---XXXXXXXXXXIWGVIRGLETWSQLFYLTNDFRELRINSTDIYDYP 406
+ G+DESY L WG +RGLET+SQ+ + T+ L + I D P
Sbjct: 111 HHGVDESYKLSIPIGSFSAHLLAHSAWGAMRGLETFSQMIWGTSPDLCLPVGIY-IQDSP 169
Query: 407 RYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLG 586
+ HRG+LLDTSR+Y + I++ I AM+ANK+NV HWHI D QSFP P L+ G
Sbjct: 170 LFGHRGVLLDTSRNYYGVDDIMRTIKAMSANKLNVFHWHITDSQSFPLVLPSEPSLAAKG 229
Query: 587 AYHPTLVYTKRDIEIVVKHAAERGVRVLTEVD 682
+ P +VYT D+ +V++ E GVRVL E+D
Sbjct: 230 SLGPDMVYTPEDVSKIVQYGFEHGVRVLPEID 261
>At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein
similar to beta-hexosaminidase A SP:P13723 from
[Dictyostelium discoideum]
Length = 541
Score = 117 bits (281), Expect = 8e-27
Identities = 68/176 (38%), Positives = 98/176 (55%), Gaps = 10/176 (5%)
Frame = +2
Query: 188 VQELHVELTAPCEKLPYFGMDESYNLXXXX--------XXXXXXXXIWGVIRGLETWSQL 343
+ L + + + E+L G+DESY L ++G +RGLET+SQL
Sbjct: 102 ITSLKIVVHSDSEELQ-LGVDESYTLMVSKKNEQSIVGAATIEANTVYGALRGLETFSQL 160
Query: 344 FYLTNDFRELRINSTDIY--DYPRYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLH 517
+ ++I Y D PR+ +RGLL+DTSRHYL + I + I++M+ K+NVLH
Sbjct: 161 CAFDYITKSVQIYKAPWYIQDKPRFGYRGLLIDTSRHYLPIDVIKQIIESMSFAKLNVLH 220
Query: 518 WHIVDDQSFPYHSEKFPELSRLGAYHPTLVYTKRDIEIVVKHAAERGVRVLTEVDV 685
WHIVD+QSFP + +P L + GAY YT D +V+ A RG+ V+ EVDV
Sbjct: 221 WHIVDEQSFPLETPTYPNLWK-GAYSRWERYTVEDASEIVRFAKMRGINVMAEVDV 275
>At2g07676.1 68415.m00907 expressed protein
Length = 118
Score = 32.7 bits (71), Expect = 0.23
Identities = 18/62 (29%), Positives = 32/62 (51%)
Frame = +2
Query: 458 LSKILKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLGAYHPTLVYTKRDIEIVV 637
+SK +K I ++ W+I+DD ++ E+F L+ + +H L Y +RD E +
Sbjct: 39 ISKYIKGILKSRLSRREQSRWNIIDDTTYMAFFEEFASLNPV--FHTFLFYGRRDGEDLS 96
Query: 638 KH 643
H
Sbjct: 97 FH 98
>At4g22260.1 68417.m03220 alternative oxidase, putative / immutans
protein (IM) identical to IMMUTANS from Arabidopsis
thaliana [gi:4138855]; contains Pfam profile PF01786
alternative oxidase
Length = 351
Score = 31.5 bits (68), Expect = 0.54
Identities = 17/42 (40%), Positives = 28/42 (66%)
Frame = -3
Query: 674 LLRLEPLSQRRVSLQSLYRVWCTLMLDDKLRVDLVRETFHCD 549
LL PLS RR+ L++ +RV T++ DD+ +V +V E+F +
Sbjct: 36 LLHHLPLSSRRLLLRNNHRVQATILQDDEEKV-VVEESFKAE 76
>At3g46530.1 68416.m05051 disease resistance protein, RPP13-like
(CC-NBS class), putative domain signature CC-NBS exists,
suggestive of a disease resistance protein. Closest
homolog in Col-0 to RPP13.
Length = 835
Score = 30.3 bits (65), Expect = 1.2
Identities = 17/54 (31%), Positives = 24/54 (44%)
Frame = -3
Query: 614 WCTLMLDDKLRVDLVRETFHCDKENSDHRRYANGVRSFYSRPWHRYSLVSSITI 453
W L+LD V+ V +T+H E RR + + R YS+V I I
Sbjct: 61 WSKLVLDFAYDVEDVLDTYHLKLEERSQRRGLRRLTNKIGRKMDAYSIVDDIRI 114
>At3g47890.1 68416.m05222 ubiquitin carboxyl-terminal
hydrolase-related contains Pfam profiles PF00443:
Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
of unknown function (DUF629), PF04781: Protein of unknown
function (DUF627)
Length = 1568
Score = 28.7 bits (61), Expect = 3.8
Identities = 28/112 (25%), Positives = 50/112 (44%), Gaps = 4/112 (3%)
Frame = +1
Query: 316 KRLGNLVATLLSYKRFQGIAYQLDGYIRLP*IRSQGTPAGHIQALFIVIEDTKEYR-CHG 492
++L NL AT+ + RF G + + + + +A+ + ++ +EYR C+
Sbjct: 924 RKLKNLKATIGNDHRFNGDSIE-HSLLSVASFGDHSEADVVSEAIEALSDEEEEYRRCNE 982
Query: 493 RE*NERT---PLAYRR*SEFSLSQ*KVSRTKSTRSLSSNISVHQTRYRDCSE 639
E ER L Y+R E + ++ K S S ++V + Y DC E
Sbjct: 983 LEEEERKLAKTLEYQRRIENEAKEKHIAEQKKKYSCSDPMNVTEAVYDDCIE 1034
>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 28.3 bits (60), Expect = 5.0
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 44 FLTKALERYQFLVRDLHRITRRFVK 118
++ K L R FL+++L +TRRF++
Sbjct: 109 YIQKILRRKPFLIKNLENVTRRFLQ 133
>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|P47823
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 28.3 bits (60), Expect = 5.0
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 44 FLTKALERYQFLVRDLHRITRRFVK 118
++ K L R FL+++L +TRRF++
Sbjct: 109 YIQKILRRKAFLIKNLENVTRRFLQ 133
>At4g16510.1 68417.m02498 YbaK/prolyl-tRNA synthetase-related
contains weak hit to Pfam profile PF04073:
YbaK/prolyl-tRNA synthetases associated domain
Length = 232
Score = 27.9 bits (59), Expect = 6.6
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = +2
Query: 356 NDFRELRINSTDIYDYPRYAHRGLLLDTSRHYLSLSKILKNIDA 487
NDF R+ +TD YD+P + R +L +S +L S +L N A
Sbjct: 66 NDFCFKRV-ATDYYDWPLESRRDVLGASSVDHLCKSIVLVNTQA 108
>At2g25730.1 68415.m03084 expressed protein
Length = 2464
Score = 27.9 bits (59), Expect = 6.6
Identities = 15/52 (28%), Positives = 26/52 (50%)
Frame = +2
Query: 53 KALERYQFLVRDLHRITRRFVKRSLGSQYRSLDDEAANDERFQGTVQELHVE 208
+AL ++ D HR R+F+ L + R+L DE + +G ++ VE
Sbjct: 915 RALAMLHQMIEDAHRGKRQFLSGKLHNLARALADEKPEVDVLKGDGSDMAVE 966
>At4g21660.1 68417.m03138 proline-rich spliceosome-associated (PSP)
family protein similar to SP|Q13435 Splicing factor 3B
subunit 2 (Spliceosome associated protein 145) (SAP 145)
(SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa
subunit) {Homo sapiens}; contains Pfam profiles PF04046:
PSP, PF04037: Domain of unknown function (DUF382)
Length = 584
Score = 27.5 bits (58), Expect = 8.8
Identities = 20/75 (26%), Positives = 33/75 (44%), Gaps = 3/75 (4%)
Frame = -3
Query: 668 RLEPLSQRRVSLQ-SLYRVWCTLMLDDKLRVDL--VRETFHCDKENSDHRRYANGVRSFY 498
R++ ++VS + + VW D KL V L R T + S R+Y G R
Sbjct: 162 RMKIAELKQVSARPDVVEVWDATSADPKLLVFLKSYRNTVPVPRHWSQKRKYLQGKRGIE 221
Query: 497 SRPWHRYSLVSSITI 453
+P+H +++ I
Sbjct: 222 KQPFHLPDFIAATGI 236
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,715,043
Number of Sequences: 28952
Number of extensions: 298479
Number of successful extensions: 722
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 703
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 717
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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