Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_E14
(558 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g25490.1 68414.m03165 serine/threonine protein phosphatase 2A... 149 1e-36
At1g13320.1 68414.m01546 serine/threonine protein phosphatase 2A... 149 2e-36
At3g25800.1 68416.m03211 serine/threonine protein phosphatase 2A... 148 3e-36
At5g44010.1 68418.m05386 expressed protein 29 2.1
At5g07140.1 68418.m00814 protein kinase family protein contains ... 28 4.9
At5g01770.1 68418.m00096 transducin family protein / WD-40 repea... 27 8.5
>At1g25490.1 68414.m03165 serine/threonine protein phosphatase 2A
(PP2A) regulatory subunit A (RCN1) identical to
phosphoprotein phosphatase 2A, regulatory subunit A
GI:1262171 from [Arabidopsis thaliana]
Length = 588
Score = 149 bits (361), Expect = 1e-36
Identities = 76/118 (64%), Positives = 92/118 (77%)
Frame = +2
Query: 98 RNLVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLEC 277
+ L D++ HV+SALASVIMG+ PI+G+ +TIE LLP+FL LKDE P+VRLNIIS L+
Sbjct: 325 KELSSDSSQHVRSALASVIMGMAPILGKDSTIEHLLPIFLSLLKDEFPDVRLNIISKLDQ 384
Query: 278 VNEVLGIQQLLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQEFFDKKLTGLCM 451
VN+V+GI L QSLLPAIV LA D WRVRLAIIE++ LLA Q+G FFD KL LCM
Sbjct: 385 VNQVIGIDLLSQSLLPAIVELAEDRHWRVRLAIIEYVPLLASQLGIGFFDDKLGALCM 442
Score = 37.9 bits (84), Expect = 0.005
Identities = 25/97 (25%), Positives = 43/97 (44%)
Frame = +2
Query: 128 VKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECVNEVLGIQQL 307
V+ A AS + V I +++ +F KD+ VRL + + ++L Q
Sbjct: 179 VRRAAASNLGKFATTVESTFLIAEIMTMFDDLTKDDQDSVRLLAVEGCAALGKLLEPQDC 238
Query: 308 LQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQE 418
+ +LP IV + D WRVR + + L +G +
Sbjct: 239 VARILPVIVNFSQDKSWRVRYMVANQLYELCEAVGPD 275
Score = 36.3 bits (80), Expect = 0.014
Identities = 28/120 (23%), Positives = 57/120 (47%)
Frame = +2
Query: 101 NLVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECV 280
N D + V+ +A+ + L VG T L+P ++ L+D EVR+ +
Sbjct: 248 NFSQDKSWRVRYMVANQLYELCEAVGPDCTRTDLVPAYVRLLRDNEAEVRIAAAGKVTKF 307
Query: 281 NEVLGIQQLLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQEFFDKKLTGLCMSVV 460
+L + +Q +LP + L+ D+ VR A+ + +A +G++ + L + +S++
Sbjct: 308 CRLLNPELAIQHILPCVKELSSDSSQHVRSALASVIMGMAPILGKDSTIEHLLPIFLSLL 367
Score = 31.9 bits (69), Expect = 0.30
Identities = 27/111 (24%), Positives = 46/111 (41%)
Frame = +2
Query: 104 LVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECVN 283
L+ D V+ A A + ++ + I+ +LP D VR + S + +
Sbjct: 288 LLRDNEAEVRIAAAGKVTKFCRLLNPELAIQHILPCVKELSSDSSQHVRSALASVIMGMA 347
Query: 284 EVLGIQQLLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQEFFDKKL 436
+LG ++ LLP + L D VRL II + + IG + + L
Sbjct: 348 PILGKDSTIEHLLPIFLSLLKDEFPDVRLNIISKLDQVNQVIGIDLLSQSL 398
Score = 29.5 bits (63), Expect = 1.6
Identities = 28/99 (28%), Positives = 50/99 (50%), Gaps = 4/99 (4%)
Frame = +2
Query: 194 EQLLPLFLI--QLKDECPEVRLNIISYLECVNEVLGIQQLLQSLLPAIV*LAYDTKWRVR 367
E L P+ ++ +LK++ ++RLN I L + LG ++ + L+P + + D V
Sbjct: 6 EPLYPIAVLIDELKNDDIQLRLNSIRRLSTIARALGEERTRKELIPFLSENSDDDD-EVL 64
Query: 368 LAIIEHMALLAGQIGQ-EFFDKKLTGL-CMSVVD*SCVR 478
LA+ E + + +G EF L L + V+ +CVR
Sbjct: 65 LAMAEELGVFIPFVGGIEFAHVLLPPLESLCTVEETCVR 103
Score = 29.1 bits (62), Expect = 2.1
Identities = 22/91 (24%), Positives = 41/91 (45%)
Frame = +2
Query: 101 NLVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECV 280
+L D V+ L ++ Q+ + ++LP+ + +D+ VR + + L +
Sbjct: 209 DLTKDDQDSVRLLAVEGCAALGKLLEPQDCVARILPVIVNFSQDKSWRVRYMVANQLYEL 268
Query: 281 NEVLGIQQLLQSLLPAIV*LAYDTKWRVRLA 373
E +G L+PA V L D + VR+A
Sbjct: 269 CEAVGPDCTRTDLVPAYVRLLRDNEAEVRIA 299
>At1g13320.1 68414.m01546 serine/threonine protein phosphatase 2A
(PP2A) 65 kDa regulatory subunit, putative similar to
protein phosphatase 2A 65 kDa regulatory subunit
GI:683502 from [Arabidopsis thaliana]
Length = 587
Score = 149 bits (360), Expect = 2e-36
Identities = 75/118 (63%), Positives = 92/118 (77%)
Frame = +2
Query: 98 RNLVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLEC 277
+ L D++ HV+SALASVIMG+ P++G+ TIE LLP+FL LKDE P+VRLNIIS L+
Sbjct: 325 KELSSDSSQHVRSALASVIMGMAPVLGKDATIEHLLPIFLSLLKDEFPDVRLNIISKLDQ 384
Query: 278 VNEVLGIQQLLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQEFFDKKLTGLCM 451
VN+V+GI L QSLLPAIV LA D WRVRLAIIE++ LLA Q+G FFD+KL LCM
Sbjct: 385 VNQVIGIDLLSQSLLPAIVELAEDRHWRVRLAIIEYIPLLASQLGVGFFDEKLGALCM 442
Score = 37.5 bits (83), Expect = 0.006
Identities = 30/111 (27%), Positives = 47/111 (42%)
Frame = +2
Query: 104 LVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECVN 283
L+CD V+ A A + I+ + I+ +LP D VR + S + +
Sbjct: 288 LLCDNEAEVRIAAAGKVTKFCRILNPELAIQHILPCVKELSSDSSQHVRSALASVIMGMA 347
Query: 284 EVLGIQQLLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQEFFDKKL 436
VLG ++ LLP + L D VRL II + + IG + + L
Sbjct: 348 PVLGKDATIEHLLPIFLSLLKDEFPDVRLNIISKLDQVNQVIGIDLLSQSL 398
Score = 35.9 bits (79), Expect = 0.018
Identities = 28/120 (23%), Positives = 56/120 (46%)
Frame = +2
Query: 101 NLVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECV 280
N D + V+ +A+ + L VG + T L+P + L D EVR+ +
Sbjct: 248 NFSQDKSWRVRYMVANQLYELCEAVGPEPTRTDLVPAYARLLCDNEAEVRIAAAGKVTKF 307
Query: 281 NEVLGIQQLLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQEFFDKKLTGLCMSVV 460
+L + +Q +LP + L+ D+ VR A+ + +A +G++ + L + +S++
Sbjct: 308 CRILNPELAIQHILPCVKELSSDSSQHVRSALASVIMGMAPVLGKDATIEHLLPIFLSLL 367
Score = 35.1 bits (77), Expect = 0.032
Identities = 19/73 (26%), Positives = 34/73 (46%)
Frame = +2
Query: 200 LLPLFLIQLKDECPEVRLNIISYLECVNEVLGIQQLLQSLLPAIV*LAYDTKWRVRLAII 379
++ +F +D+ VRL + + ++L Q + +LP IV + D WRVR +
Sbjct: 203 IMSMFEDLTQDDQDSVRLLAVEGCAALGKLLEPQDCVAHILPVIVNFSQDKSWRVRYMVA 262
Query: 380 EHMALLAGQIGQE 418
+ L +G E
Sbjct: 263 NQLYELCEAVGPE 275
Score = 29.9 bits (64), Expect = 1.2
Identities = 20/101 (19%), Positives = 45/101 (44%)
Frame = +2
Query: 113 DANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECVNEVL 292
D H ++ A A+ + L G + ++ ++P L + + R+ I+ + + V+
Sbjct: 447 DKVHSIREAAANNLKRLAEEFGPEWAMQHIVPQVLEMINNPHYLYRMTILRAVSLLAPVM 506
Query: 293 GIQQLLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQ 415
G + LLPA++ + D ++ + + M L + Q
Sbjct: 507 GSEITCSKLLPAVITASKDRVPNIKFNVAKMMQSLIPIVDQ 547
Score = 28.7 bits (61), Expect = 2.8
Identities = 21/91 (23%), Positives = 40/91 (43%)
Frame = +2
Query: 101 NLVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECV 280
+L D V+ L ++ Q+ + +LP+ + +D+ VR + + L +
Sbjct: 209 DLTQDDQDSVRLLAVEGCAALGKLLEPQDCVAHILPVIVNFSQDKSWRVRYMVANQLYEL 268
Query: 281 NEVLGIQQLLQSLLPAIV*LAYDTKWRVRLA 373
E +G + L+PA L D + VR+A
Sbjct: 269 CEAVGPEPTRTDLVPAYARLLCDNEAEVRIA 299
>At3g25800.1 68416.m03211 serine/threonine protein phosphatase 2A
(PP2A) 65 KDa regulatory subunit A identical to protein
phosphatase 2A 65 kDa regulatory subunit (pDF1)
GI:683502 from [Arabidopsis thaliana]
Length = 587
Score = 148 bits (358), Expect = 3e-36
Identities = 75/118 (63%), Positives = 91/118 (77%)
Frame = +2
Query: 98 RNLVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLEC 277
+ L D++ HV+SALASVIMG+ P++G+ TIE LLP+FL LKDE P+VRLNIIS L+
Sbjct: 325 KELSSDSSQHVRSALASVIMGMAPVLGKDATIEHLLPIFLSLLKDEFPDVRLNIISKLDQ 384
Query: 278 VNEVLGIQQLLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQEFFDKKLTGLCM 451
VN+V+GI L QSLLPAIV LA D WRVRLAIIE++ LLA Q+G FFD KL LCM
Sbjct: 385 VNQVIGIDLLSQSLLPAIVELAEDRHWRVRLAIIEYIPLLASQLGVGFFDDKLGALCM 442
Score = 39.1 bits (87), Expect = 0.002
Identities = 28/120 (23%), Positives = 59/120 (49%)
Frame = +2
Query: 101 NLVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECV 280
N D + V+ +A+ + L VG + T +L+P ++ L+D EVR+ +
Sbjct: 248 NFSQDKSWRVRYMVANQLYELCEAVGPEPTRTELVPAYVRLLRDNEAEVRIAAAGKVTKF 307
Query: 281 NEVLGIQQLLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQEFFDKKLTGLCMSVV 460
+L + +Q +LP + L+ D+ VR A+ + +A +G++ + L + +S++
Sbjct: 308 CRILNPEIAIQHILPCVKELSSDSSQHVRSALASVIMGMAPVLGKDATIEHLLPIFLSLL 367
Score = 37.1 bits (82), Expect = 0.008
Identities = 20/73 (27%), Positives = 35/73 (47%)
Frame = +2
Query: 200 LLPLFLIQLKDECPEVRLNIISYLECVNEVLGIQQLLQSLLPAIV*LAYDTKWRVRLAII 379
++ +F +D+ VRL + + ++L Q +Q +LP IV + D WRVR +
Sbjct: 203 VMSMFEDLTQDDQDSVRLLAVEGCAALGKLLEPQDCVQHILPVIVNFSQDKSWRVRYMVA 262
Query: 380 EHMALLAGQIGQE 418
+ L +G E
Sbjct: 263 NQLYELCEAVGPE 275
Score = 32.7 bits (71), Expect = 0.17
Identities = 29/111 (26%), Positives = 46/111 (41%)
Frame = +2
Query: 104 LVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECVN 283
L+ D V+ A A + I+ + I+ +LP D VR + S + +
Sbjct: 288 LLRDNEAEVRIAAAGKVTKFCRILNPEIAIQHILPCVKELSSDSSQHVRSALASVIMGMA 347
Query: 284 EVLGIQQLLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQEFFDKKL 436
VLG ++ LLP + L D VRL II + + IG + + L
Sbjct: 348 PVLGKDATIEHLLPIFLSLLKDEFPDVRLNIISKLDQVNQVIGIDLLSQSL 398
Score = 31.5 bits (68), Expect = 0.39
Identities = 22/91 (24%), Positives = 42/91 (46%)
Frame = +2
Query: 101 NLVCDANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECV 280
+L D V+ L ++ Q+ ++ +LP+ + +D+ VR + + L +
Sbjct: 209 DLTQDDQDSVRLLAVEGCAALGKLLEPQDCVQHILPVIVNFSQDKSWRVRYMVANQLYEL 268
Query: 281 NEVLGIQQLLQSLLPAIV*LAYDTKWRVRLA 373
E +G + L+PA V L D + VR+A
Sbjct: 269 CEAVGPEPTRTELVPAYVRLLRDNEAEVRIA 299
Score = 30.3 bits (65), Expect = 0.91
Identities = 28/99 (28%), Positives = 50/99 (50%), Gaps = 4/99 (4%)
Frame = +2
Query: 194 EQLLPLFLI--QLKDECPEVRLNIISYLECVNEVLGIQQLLQSLLPAIV*LAYDTKWRVR 367
E L P+ ++ +LK++ ++RLN I L + LG ++ + L+P + D V
Sbjct: 6 EPLYPIAVLIDELKNDDIQLRLNSIRRLSTIARALGEERTRKELIPFLS-ENNDDDDEVL 64
Query: 368 LAIIEHMALLAGQI-GQEFFDKKLTGL-CMSVVD*SCVR 478
LA+ E + + + G E+ L L +S V+ +CVR
Sbjct: 65 LAMAEELGVFIPYVGGVEYAHVLLPPLETLSTVEETCVR 103
Score = 27.9 bits (59), Expect = 4.9
Identities = 19/108 (17%), Positives = 47/108 (43%)
Frame = +2
Query: 113 DANHHVKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECVNEVL 292
D H ++ A A+ + L G + ++ ++P L + + R+ I+ + + V+
Sbjct: 447 DKVHSIRDAAANNLKRLAEEFGPEWAMQHIVPQVLEMVNNPHYLYRMTILRAVSLLAPVM 506
Query: 293 GIQQLLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQEFFDKKL 436
G + LLP ++ + D ++ + + + L + Q +K +
Sbjct: 507 GSEITCSKLLPVVMTASKDRVPNIKFNVAKVLQSLIPIVDQSVVEKTI 554
>At5g44010.1 68418.m05386 expressed protein
Length = 357
Score = 29.1 bits (62), Expect = 2.1
Identities = 19/37 (51%), Positives = 23/37 (62%)
Frame = -1
Query: 447 QRPVSFLSKNS*PIWPASKAICSMIAKRTLHLVS*AS 337
Q+P+ L N IW SKAI ++KRTL LVS AS
Sbjct: 147 QQPIELLRWN---IWQ-SKAISYFLSKRTLRLVSGAS 179
>At5g07140.1 68418.m00814 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 583
Score = 27.9 bits (59), Expect = 4.9
Identities = 15/51 (29%), Positives = 27/51 (52%)
Frame = +2
Query: 128 VKSALASVIMGLNPIVGRQNTIEQLLPLFLIQLKDECPEVRLNIISYLECV 280
V++A+ GL P + ++ Q+L +I+ + CP RLN S + C+
Sbjct: 529 VQAAVGIAACGLRPDIPKE--CPQVLKYLMIKCWNTCPSTRLN-FSQIHCI 576
>At5g01770.1 68418.m00096 transducin family protein / WD-40 repeat
family protein similar to WD-repeat protein mip1
(SP:P87141) [Schizosaccharomyces pombe]
Length = 1354
Score = 27.1 bits (57), Expect = 8.5
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = +2
Query: 305 LLQSLLPAIV*LAYDTKWRVRLAIIEHMALLAGQIGQEFFDKKLTGLCM 451
LL P +V A D R+R+ E LL G +F DK ++ LC+
Sbjct: 1026 LLHPFSPIVV--AADENERIRVWNYEEATLLNGFDNNDFPDKGISNLCL 1072
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,886,503
Number of Sequences: 28952
Number of extensions: 232546
Number of successful extensions: 562
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 524
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 559
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1062855648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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