BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_E09
(557 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 31 0.11
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 28 1.1
SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|c... 26 3.3
SPCC1183.08c |rpl101|rpl1-1, rpl10a-1|60S ribosomal protein L10a... 26 4.3
SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 4.3
SPAC589.06c |||pho88 family protein|Schizosaccharomyces pombe|ch... 26 4.3
SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr ... 25 5.7
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 25 5.7
SPAC30D11.06c |||DUF300 family protein|Schizosaccharomyces pombe... 25 5.7
SPBC31F10.11c |cwf4|syf3|complexed with Cdc5 protein Cwf4 |Schiz... 25 5.7
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa... 25 7.5
SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc... 25 10.0
SPBC30D10.18c |rpl102|rpl1-2, rpl10a-2|60S ribosomal protein L10... 25 10.0
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 31.1 bits (67), Expect = 0.11
Identities = 23/89 (25%), Positives = 39/89 (43%)
Frame = -1
Query: 323 RSQEDLDNGISGNIGLNVDGNTERLVVESWLTNLEVVWVTSLNLFFGQEYTVSGIKLAIV 144
R QED + G N+ G E + E ++ L F G+ + +S + I+
Sbjct: 1109 RCQEDFERGWKANLPSGKAGEAEIMSDEYYVAAAIKRRGLGLVRFIGELFKLSMLSEKIM 1168
Query: 143 KECD*FLNDNIIDFNADEMKFLLRIRLQV 57
EC L N+ D +E++ L R+ + V
Sbjct: 1169 HECIKRLLGNVTDPEEEEIESLCRLLMTV 1197
>SPAC26A3.09c |rga2||GTPase activating protein
Rga2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1275
Score = 27.9 bits (59), Expect = 1.1
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 2/45 (4%)
Frame = +3
Query: 375 FYELDWFTHKITPGQNKIVRNSNE--FSLFKEDSLPLTDLMKLLD 503
F LDWFT+ + QN+++R S + F + L L +KLL+
Sbjct: 654 FPTLDWFTNDSSVIQNELLRRSVDTYFRYIFQTDLKLEQRIKLLE 698
>SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 796
Score = 26.2 bits (55), Expect = 3.3
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +3
Query: 303 IKIFLGPKYNDDGFPITLEENWHKFYELDWFTHKIT 410
+ I L P ND + T WHKF++ WF +T
Sbjct: 416 LHIELAPAANDIQWHNTYIGRWHKFFQ-GWFITLVT 450
>SPCC1183.08c |rpl101|rpl1-1, rpl10a-1|60S ribosomal protein
L10a|Schizosaccharomyces pombe|chr 3|||Manual
Length = 216
Score = 25.8 bits (54), Expect = 4.3
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = -2
Query: 517 GTFPSSNNFMRSVNGKESSLKSENSFELRTILFCPGV 407
G FPS + + GK + +KS F+L+ +L C GV
Sbjct: 131 GKFPSPVSHADDLYGKITEVKSTIKFQLKKVL-CLGV 166
>SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 682
Score = 25.8 bits (54), Expect = 4.3
Identities = 16/54 (29%), Positives = 27/54 (50%)
Frame = +3
Query: 189 KEIKTSYPHNFKVRQPRLNHKPFSVTIDVKSDIATDAVIKIFLGPKYNDDGFPI 350
+ I+ SYP+ F R P+ H P + +D + A + V L P+ N + P+
Sbjct: 606 QHIQYSYPNTFVNRFPQNIHHPSANLLDASA--ALNPVQNPLLMPQQNHEHSPL 657
>SPAC589.06c |||pho88 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 202
Score = 25.8 bits (54), Expect = 4.3
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = -2
Query: 556 LFGMHSKPSDIYNGTFPSSNNFMRSVNGKESSLKSENSFE 437
LFG +KP+ GT +SNN +G + +EN E
Sbjct: 160 LFGGGNKPAAAVTGTSSNSNNASAKSDGPTITELNENETE 199
>SPAC6F6.01 |||VIC sodium channel |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1854
Score = 25.4 bits (53), Expect = 5.7
Identities = 14/51 (27%), Positives = 26/51 (50%), Gaps = 3/51 (5%)
Frame = -1
Query: 278 LNVDGNTERLVVESWLTNLEVVWVTSLNLFFGQEYTVSGI---KLAIVKEC 135
LN+ TE ++ ++ +V V S N FFG + G+ K ++ ++C
Sbjct: 309 LNITRKTETILKSLKESSTPLVQVVSFNAFFGVMIAILGVQFFKASLNRQC 359
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 25.4 bits (53), Expect = 5.7
Identities = 17/47 (36%), Positives = 24/47 (51%), Gaps = 1/47 (2%)
Frame = -2
Query: 370 CQFSSNVMGNPSSLYLGPKKILITASVAISDLTSMV-TLKGLWLSRG 233
CQF V+G L+ K LI+ S A L+++ + KG LS G
Sbjct: 113 CQFWETVLGMECRLHQLRNKELISVSAASKILSNITYSYKGYGLSMG 159
>SPAC30D11.06c |||DUF300 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 426
Score = 25.4 bits (53), Expect = 5.7
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +1
Query: 322 LSTTMMDSPSHWKKTGINSTNWIG 393
LS + S+W++T ++ TNW+G
Sbjct: 208 LSVKAIIFASYWQQTVLSITNWLG 231
>SPBC31F10.11c |cwf4|syf3|complexed with Cdc5 protein Cwf4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 674
Score = 25.4 bits (53), Expect = 5.7
Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 1/59 (1%)
Frame = +3
Query: 123 EKLVTFFDYSQFDATNSVFLTKKEIKTSYPHNFKVRQPRLNHKPFSVTID-VKSDIATD 296
E++V + QF+A + T+K + + P K R+ RL F +D + D ATD
Sbjct: 591 ERVVLLEAWKQFEAMHGTEDTRKHVSSLMPQVVKKRR-RLEDGSFEEYLDYLFPDTATD 648
>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 25.0 bits (52), Expect = 7.5
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +1
Query: 4 HSISYITGLWVTLTHSSIT*SLILKRNFISSALKSMMLSLRN*SH 138
+S S I W T HSS+ +L R + S + ++ LRN H
Sbjct: 973 NSKSVIGENWTTCLHSSLVDNLGKYRKYDGSKILDILRVLRNKRH 1017
>SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 581
Score = 24.6 bits (51), Expect = 10.0
Identities = 12/43 (27%), Positives = 25/43 (58%), Gaps = 4/43 (9%)
Frame = +3
Query: 15 LYNRIVGYINAFKHYLKP----YPQEKLHFVGVKINDVVVEKL 131
+Y +++G +N F+ ++ Y + + VKI+ +VV+KL
Sbjct: 385 VYYKLIGILNRFRKSVQRQEENYVNTRSTILSVKIHHIVVQKL 427
>SPBC30D10.18c |rpl102|rpl1-2, rpl10a-2|60S ribosomal protein
L10a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 216
Score = 24.6 bits (51), Expect = 10.0
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = -2
Query: 517 GTFPSSNNFMRSVNGKESSLKSENSFELRTILFCPGV 407
G FPS + + GK +KS F+L+ +L C GV
Sbjct: 131 GKFPSPVSHSDDLYGKIIEVKSTIKFQLKKVL-CLGV 166
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,255,047
Number of Sequences: 5004
Number of extensions: 45946
Number of successful extensions: 147
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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