Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_D19
(541 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 236 2e-63
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 133 1e-32
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 128 4e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 122 3e-29
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.3
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 1.3
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.8
SPAC1751.02c |rsm19||mitochondrial ribosomal protein subunit S19... 25 5.4
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 5.4
SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 9.5
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 9.5
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 236 bits (577), Expect = 2e-63
Identities = 101/134 (75%), Positives = 118/134 (88%)
Frame = +2
Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 244
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 245 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGXNWAKGHYTEGAELVDSVLDVV 424
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAG WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 425 RKESESCDCLQGFQ 466
R+E+E+CD LQGFQ
Sbjct: 121 RREAEACDALQGFQ 134
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 133 bits (322), Expect = 1e-32
Identities = 61/135 (45%), Positives = 85/135 (62%), Gaps = 2/135 (1%)
Frame = +2
Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 238
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 239 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGXNWAKGHYTEGAELVDSVLD 418
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A N+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 419 VVRKESESCDCLQGF 463
+R+ +++C LQGF
Sbjct: 121 KIRRIADNCSGLQGF 135
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 128 bits (310), Expect = 4e-31
Identities = 58/139 (41%), Positives = 86/139 (61%), Gaps = 6/139 (4%)
Frame = +2
Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 226
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 227 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGXNWAKGHYTEGAELVD 406
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A N+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 407 SVLDVVRKESESCDCLQGF 463
SVL+ +R+ +++C LQGF
Sbjct: 121 SVLERIRRMADNCSGLQGF 139
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 122 bits (295), Expect = 3e-29
Identities = 51/134 (38%), Positives = 90/134 (67%), Gaps = 2/134 (1%)
Frame = +2
Query: 68 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 247
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 248 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGXNWAKGHYTEGAELVDSVLDV 421
RAIL+DLEP ++++ S +G ++ P+N + ++ GAG NWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 422 VRKESESCDCLQGF 463
+ +E++ D L+GF
Sbjct: 122 IDREADGSDSLEGF 135
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 1.3
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +2
Query: 368 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 457
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 27.5 bits (58), Expect = 1.3
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +2
Query: 299 GPFGQIFRPDNFVFGQSGAG-XNWAKGHYTEGAEL 400
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 1.8
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 104 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 205
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC1751.02c |rsm19||mitochondrial ribosomal protein subunit
S19|Schizosaccharomyces pombe|chr 1|||Manual
Length = 108
Score = 25.4 bits (53), Expect = 5.4
Identities = 17/60 (28%), Positives = 25/60 (41%)
Frame = +3
Query: 354 PVTTGPRDTTQRALS*STQFLT*SEKNPNHVIVYKDSNSHTLSEAVPAPVWAHYFISKIR 533
P+ T R T QF+ + K+ +V + +D H L E P HY +K R
Sbjct: 49 PIKTAVRSATILPRMVGAQFMVHNGKSYANVKITEDMIGHKLGEFAPTRKAFHYRQTKNR 108
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 5.4
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 357 PAPDCPKTKLSGRNICPKGPERTESMVPGSRS 262
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPBC3B8.04c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 867
Score = 24.6 bits (51), Expect = 9.5
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -2
Query: 324 GRNICPKGPERTESMVPGSRSTRMARGTYLP 232
G NI PER + +S RMAR LP
Sbjct: 106 GNNINYPTPERLQKSSASRKSGRMARSQELP 136
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 24.6 bits (51), Expect = 9.5
Identities = 12/44 (27%), Positives = 19/44 (43%)
Frame = +2
Query: 320 RPDNFVFGQSGAGXNWAKGHYTEGAELVDSVLDVVRKESESCDC 451
RP +F G + G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,960,807
Number of Sequences: 5004
Number of extensions: 36302
Number of successful extensions: 107
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 104
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 221892220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -