Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_D01
(653 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g37720.1 68418.m04541 RNA and export factor-binding protein, ... 33 0.13
At5g13480.1 68418.m01554 WD-40 repeat family protein similar to ... 31 0.67
At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase fa... 31 0.67
At4g22670.1 68417.m03272 tetratricopeptide repeat (TPR)-containi... 30 1.5
At3g57630.2 68416.m06421 exostosin family protein contains Pfam ... 29 2.0
At3g57630.1 68416.m06420 exostosin family protein contains Pfam ... 29 2.0
At3g53970.1 68416.m05963 proteasome inhibitor-related similar to... 29 2.0
At5g01950.1 68418.m00114 leucine-rich repeat transmembrane prote... 29 3.6
At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein si... 29 3.6
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 29 3.6
At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR... 28 4.7
At4g02430.1 68417.m00329 pre-mRNA splicing factor, putative / SR... 28 4.7
At1g64140.1 68414.m07266 expressed protein similar to putative d... 28 4.7
At1g15190.1 68414.m01816 hypothetical protein 28 4.7
At5g09670.2 68418.m01119 loricrin-related contains weak similari... 27 8.2
At5g09670.1 68418.m01118 loricrin-related contains weak similari... 27 8.2
At3g15820.1 68416.m02002 phosphatidic acid phosphatase-related /... 27 8.2
>At5g37720.1 68418.m04541 RNA and export factor-binding protein,
putative transcriptional coactivator ALY, Mus musculus,
EMBL:MMU89876
Length = 288
Score = 33.5 bits (73), Expect = 0.13
Identities = 17/47 (36%), Positives = 21/47 (44%)
Frame = -1
Query: 524 SRCTPAFPCKSRSTRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 384
SR P + R RGG RG GRGRG G + P+ +S
Sbjct: 223 SRRLPIHNQQGGGMRGGRGGFRARGRGNGGRGRGGGRGNGKKPVEKS 269
>At5g13480.1 68418.m01554 WD-40 repeat family protein similar to
WD-repeat protein WDC146 (SP:Q9C0J8|) {Homo sapiens};
contains 3 weak Pfam PF00400: WD domain, G-beta repeats;
Length = 711
Score = 31.1 bits (67), Expect = 0.67
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +1
Query: 427 LPRPLGAVPRGAVPPRADRVLRDLQGNAGVQRERNVRLPAAH 552
LPRP+ P G +PP + + + G+ G+Q N ++ +H
Sbjct: 608 LPRPMQMPPHGHMPPPSMPMSHQMPGSMGMQGGMNPQMSQSH 649
>At3g56600.1 68416.m06294 phosphatidylinositol 3- and 4-kinase
family protein low similarity to 55 kDa type II
phosphatidylinositol 4-kinase [Rattus norvegicus]
GI:13660755; contains Pfam profile PF00454:
Phosphatidylinositol 3- and 4-kinase
Length = 533
Score = 31.1 bits (67), Expect = 0.67
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = -3
Query: 444 SEWSRQRQRCRYTSQSDDQVSLKFVHTLRLESVSYGHSLMQLQIASPCSLIS 289
S++SR QRCR S ++ + +T + + HSL +++PC IS
Sbjct: 13 SQFSRSSQRCRLQSLTNLDFNFLGFNTKQTNLSASSHSLNNRSVSTPCFSIS 64
>At4g22670.1 68417.m03272 tetratricopeptide repeat (TPR)-containing
protein similar to Hsc70-interacting protein (Hip) from
{Homo sapiens} SP|P50502, {Rattus norvegicus} SP|P50503;
contains Pfam profile PF00515: tetratricopeptide repeat
(TPR) domain
Length = 441
Score = 29.9 bits (64), Expect = 1.5
Identities = 21/52 (40%), Positives = 25/52 (48%), Gaps = 1/52 (1%)
Frame = +1
Query: 73 GAADVGGASPRGHLRVGAPRERHGGEPGRETIPGG*E-SSCLRDPE*VTRCS 225
G +GG P G G P GG PG +PGG + S L DPE +T S
Sbjct: 349 GMPGMGGGMPAGMGGGGMPGAG-GGMPGGGGMPGGMDFSKILNDPELMTAFS 399
>At3g57630.2 68416.m06421 exostosin family protein contains Pfam
profile: PF03016 exostosin family
Length = 791
Score = 29.5 bits (63), Expect = 2.0
Identities = 17/68 (25%), Positives = 27/68 (39%), Gaps = 1/68 (1%)
Frame = +2
Query: 209 ESRDAPDNEINLDDPC-MKVHCSAGRVCEINEHGEAICNCIKECPYETDSRRKVCTNFNE 385
+ DA ++ + + C K C GR CEI C C+ +C R C
Sbjct: 240 DPEDAYAMKVKIKEECDCKYDCLWGRFCEIPVQ----CTCVNQCSGHGKCRGGFCQCDKG 295
Query: 386 TWSSDCEV 409
+ +DC +
Sbjct: 296 WFGTDCSI 303
>At3g57630.1 68416.m06420 exostosin family protein contains Pfam
profile: PF03016 exostosin family
Length = 793
Score = 29.5 bits (63), Expect = 2.0
Identities = 17/68 (25%), Positives = 27/68 (39%), Gaps = 1/68 (1%)
Frame = +2
Query: 209 ESRDAPDNEINLDDPC-MKVHCSAGRVCEINEHGEAICNCIKECPYETDSRRKVCTNFNE 385
+ DA ++ + + C K C GR CEI C C+ +C R C
Sbjct: 242 DPEDAYAMKVKIKEECDCKYDCLWGRFCEIPVQ----CTCVNQCSGHGKCRGGFCQCDKG 297
Query: 386 TWSSDCEV 409
+ +DC +
Sbjct: 298 WFGTDCSI 305
>At3g53970.1 68416.m05963 proteasome inhibitor-related similar to
proteasome inhibitor PI31 subunit (hPI31) SP:Q92530 from
[Homo sapiens]
Length = 302
Score = 29.5 bits (63), Expect = 2.0
Identities = 16/39 (41%), Positives = 17/39 (43%)
Frame = +1
Query: 61 YDDYGAADVGGASPRGHLRVGAPRERHGGEPGRETIPGG 177
+D YG V G P G PR GG P E PGG
Sbjct: 261 FDPYGPPGVPGFEP-GRFTRQPPRGPGGGHPDLEHFPGG 298
>At5g01950.1 68418.m00114 leucine-rich repeat transmembrane protein
kinase, putative receptor protein kinases
Length = 1032
Score = 28.7 bits (61), Expect = 3.6
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = -3
Query: 249 SSRLISLSGASRDSFRISETRALSASWNRLSSRLSAMPFSR 127
S R SL GA D +I + L SWN L+ + + FS+
Sbjct: 334 SLRNCSLKGALPDFSKIRHLKYLDLSWNELTGPIPSSNFSK 374
>At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein
similar to lysosomal alpha-mannosidase GI:3522867 from
[Homo sapiens]
Length = 1019
Score = 28.7 bits (61), Expect = 3.6
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = +2
Query: 128 LENGMAESLDERRFQEAERARVSEILNESRDAPD 229
LENG E + RR Q + V EILNE+ P+
Sbjct: 794 LENGQIELMLHRRMQHDDIRGVGEILNETVCLPE 827
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -1
Query: 515 TPAFPCKSRSTRSARGGTAPRGTAPSGRGRGSA 417
+PA C S ++ S+ T + PSGR R SA
Sbjct: 40 SPALRCASAASSSSSSATTAETSKPSGRNRRSA 72
>At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR1
protein, putative strong similarity to SP|O22315
Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis
thaliana}; cDNA NCBI_gi:15810292 supports a truncated
version while protein evidence supports a longer model.
Length = 278
Score = 28.3 bits (60), Expect = 4.7
Identities = 16/36 (44%), Positives = 18/36 (50%)
Frame = -1
Query: 491 RSTRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 384
RS+ ARG + RG GRG G G R P RS
Sbjct: 85 RSSHDARGSYSGRGRG--GRGGGDGGGRERGPSRRS 118
Score = 27.9 bits (59), Expect = 6.2
Identities = 24/61 (39%), Positives = 32/61 (52%), Gaps = 1/61 (1%)
Frame = -1
Query: 560 SRACAAGSLTFRSRCTPAFPCKSRS-TRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 384
SR+ + G +SR P +SRS +RS +P+ A S R R A T RSP +RS
Sbjct: 199 SRSPSRGRSYSKSRSRGRSPSRSRSRSRSRSKSRSPK--AKSLR-RSPAKSTSRSPRSRS 255
Query: 383 R 381
R
Sbjct: 256 R 256
>At4g02430.1 68417.m00329 pre-mRNA splicing factor, putative / SR1
protein, putative strong similarity to SP|O22315
Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis
thaliana}; cDNA NCBI_gi:15810292 supports a truncated
version while protein evidence supports a longer model.
Length = 178
Score = 28.3 bits (60), Expect = 4.7
Identities = 16/36 (44%), Positives = 18/36 (50%)
Frame = -1
Query: 491 RSTRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 384
RS+ ARG + RG GRG G G R P RS
Sbjct: 85 RSSHDARGSYSGRGRG--GRGGGDGGGRERGPSRRS 118
>At1g64140.1 68414.m07266 expressed protein similar to putative
disease resistance protein GB:CAB40943 GI:4586107 from
[Arabidopsis thaliana]; weak similarity to Loricrin
(Swiss-Prot:P23490) [Homo sapiens]
Length = 646
Score = 28.3 bits (60), Expect = 4.7
Identities = 19/62 (30%), Positives = 24/62 (38%), Gaps = 6/62 (9%)
Frame = -1
Query: 293 SHRHGRRCSALSCTD--RQD*FHCQEH----RVTHSGSRRHELSQPPGIVSRPGSPPCRS 132
SH GRRC + CT + C+ H R THSG + P G C
Sbjct: 377 SHGGGRRCQSNGCTKGAQGSTMFCKAHGGGKRCTHSGCTKGAEGSTPFCKGHGGGKRCAF 436
Query: 131 RG 126
+G
Sbjct: 437 QG 438
>At1g15190.1 68414.m01816 hypothetical protein
Length = 248
Score = 28.3 bits (60), Expect = 4.7
Identities = 17/57 (29%), Positives = 30/57 (52%)
Frame = -3
Query: 258 MHGSSRLISLSGASRDSFRISETRALSASWNRLSSRLSAMPFSRCSDSEMSPRGSPA 88
+HG + L+ L+ S + + ++ AL+ S L SR S P + ++SP SP+
Sbjct: 159 VHGLADLLPLTAPSSPNRLVEDSTALAKSPWFLGSRFSPAPEPYFAFMDLSPAESPS 215
>At5g09670.2 68418.m01119 loricrin-related contains weak similarity
to Loricrin (Swiss-Prot:P23490) [Homo sapiens]
Length = 546
Score = 27.5 bits (58), Expect = 8.2
Identities = 18/62 (29%), Positives = 27/62 (43%)
Frame = -1
Query: 293 SHRHGRRCSALSCTDRQD*FHCQEHRVTHSGSRRHELSQPPGIVSRPGSPPCRSRGAPTR 114
+H G+RC L CT + + ++H G RR E + +R S C G +
Sbjct: 199 THGGGKRCEHLGCTKSAE--GKTDFCISHGGGRRCEFLEGCDKAARGRSGLCIKHGG-GK 255
Query: 113 RC 108
RC
Sbjct: 256 RC 257
>At5g09670.1 68418.m01118 loricrin-related contains weak similarity
to Loricrin (Swiss-Prot:P23490) [Homo sapiens]
Length = 546
Score = 27.5 bits (58), Expect = 8.2
Identities = 18/62 (29%), Positives = 27/62 (43%)
Frame = -1
Query: 293 SHRHGRRCSALSCTDRQD*FHCQEHRVTHSGSRRHELSQPPGIVSRPGSPPCRSRGAPTR 114
+H G+RC L CT + + ++H G RR E + +R S C G +
Sbjct: 199 THGGGKRCEHLGCTKSAE--GKTDFCISHGGGRRCEFLEGCDKAARGRSGLCIKHGG-GK 255
Query: 113 RC 108
RC
Sbjct: 256 RC 257
>At3g15820.1 68416.m02002 phosphatidic acid phosphatase-related /
PAP2-related contains Pfam profile PF01569: PAP2
superfamily
Length = 301
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/30 (46%), Positives = 16/30 (53%)
Frame = +2
Query: 119 SEHLENGMAESLDERRFQEAERARVSEILN 208
S H+ M SLD RR Q A V +ILN
Sbjct: 214 SGHVAGSMIASLDMRRMQRLRLAMVFDILN 243
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,089,238
Number of Sequences: 28952
Number of extensions: 280048
Number of successful extensions: 1166
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1076
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1164
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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