Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_C24
(558 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|c... 28 1.1
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 27 2.5
SPAC513.05 |ams1||alpha-mannosidase |Schizosaccharomyces pombe|c... 26 3.3
SPCC1322.13 |ade6|min1|phosphoribosylaminoimidazole carboxylase ... 26 4.3
SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc... 26 4.3
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c... 26 4.3
SPCC645.08c |snd1||RNA-binding protein Snd1|Schizosaccharomyces ... 25 5.7
SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom... 25 5.7
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 7.5
SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar... 25 7.5
SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 7.5
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 25 7.5
SPBC1271.04c |||deoxyhypusine synthase|Schizosaccharomyces pombe... 25 7.5
SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/ diacylg... 25 10.0
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 25 10.0
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 25 10.0
>SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 577
Score = 27.9 bits (59), Expect = 1.1
Identities = 11/21 (52%), Positives = 15/21 (71%), Gaps = 1/21 (4%)
Frame = +2
Query: 353 CQH-RNCIQCRPSLCCCESFA 412
C+H R+CI+ PSL CC F+
Sbjct: 163 CKHWRDCIKKEPSLFCCLDFS 183
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 26.6 bits (56), Expect = 2.5
Identities = 20/82 (24%), Positives = 28/82 (34%)
Frame = -1
Query: 429 SGHAEYAKLSQQHKLGRHWIQFLCWQRWTGIRRSRLEGCWYTCWERQQPEXXXXXXXXXX 250
S H Y QH+ + LC + + C YTC ++ P+
Sbjct: 399 SVHEVYGHKFLQHQFYQIMRCALCGEFLKNAAGMQCIDCHYTCHKKCYPKVVTKCISKSS 458
Query: 249 X*PSSRKAVINDKISHRTGSTT 184
SS IN +I H S T
Sbjct: 459 DSASSEYEKINHRIPHHFESHT 480
>SPAC513.05 |ams1||alpha-mannosidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1077
Score = 26.2 bits (55), Expect = 3.3
Identities = 13/50 (26%), Positives = 26/50 (52%)
Frame = -1
Query: 510 IPKHTSAIRDYPSYGNTYCLLYHRPMHSGHAEYAKLSQQHKLGRHWIQFL 361
I K T+A + PS+ +HR ++ AE KL ++ ++ H +++
Sbjct: 535 ILKRTNAGQTLPSWNGELYFEFHRGTYTTQAELKKLMRKVEIALHDAEYV 584
>SPCC1322.13 |ade6|min1|phosphoribosylaminoimidazole carboxylase
Ade6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 552
Score = 25.8 bits (54), Expect = 4.3
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -2
Query: 380 DIGYNFCVGSDGLAYEGRGWKVV 312
+ GY F + S LAY+GRG VV
Sbjct: 136 EFGYPFVLKSKTLAYDGRGNYVV 158
>SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 488
Score = 25.8 bits (54), Expect = 4.3
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1
Query: 123 SGSSTVYVPFYLETFSSWTCGVQT 52
S T+YV Y+++ SW C QT
Sbjct: 435 SDGETLYVHIYVKSSKSWRCMSQT 458
>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1111
Score = 25.8 bits (54), Expect = 4.3
Identities = 12/39 (30%), Positives = 19/39 (48%)
Frame = +3
Query: 303 MYTNNLPASTFVCQSIAANTEIVSNVAPVYAVVKVLHTL 419
+Y +N+P F+C AAN ++ VV L T+
Sbjct: 342 IYESNVPNELFICTRRAANNMAYQKLSKERGVVSELGTI 380
>SPCC645.08c |snd1||RNA-binding protein Snd1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 878
Score = 25.4 bits (53), Expect = 5.7
Identities = 8/18 (44%), Positives = 14/18 (77%)
Frame = -2
Query: 263 IGDWRDDLPPEKQLSTTK 210
+G W D +PPEK+ ++T+
Sbjct: 621 VGMWHDYVPPEKKAASTE 638
>SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1562
Score = 25.4 bits (53), Expect = 5.7
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +1
Query: 499 VFRNLVSVGGFAPQHSREQN 558
+FRNL SVGGFA QH +N
Sbjct: 695 LFRNL-SVGGFAWQHVGAEN 713
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 25.0 bits (52), Expect = 7.5
Identities = 12/28 (42%), Positives = 14/28 (50%), Gaps = 1/28 (3%)
Frame = -1
Query: 477 PSYGNTYCLLYHRPMHS-GHAEYAKLSQ 397
P N YC LY P +S H Y +L Q
Sbjct: 117 PMLANLYCALYPNPGYSLCHESYFELKQ 144
>SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large
subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1689
Score = 25.0 bits (52), Expect = 7.5
Identities = 12/32 (37%), Positives = 15/32 (46%)
Frame = -2
Query: 239 PPEKQLSTTKSLIAQGVQLGVISSEYKLIGHN 144
PP+ L T +I +GV L I Y I N
Sbjct: 1531 PPDSALDTVPKVITEGVNLKAIWEFYNEISMN 1562
>SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 268
Score = 25.0 bits (52), Expect = 7.5
Identities = 12/48 (25%), Positives = 22/48 (45%)
Frame = -3
Query: 226 SYQRQNLSSHREYNXXXXXXXXXXXGTIKPWQLNVREQHCLRTFLPGN 83
SY+ +LSSHR+Y +K + + ++ L +L G+
Sbjct: 128 SYENPSLSSHRQYQIPTPIQLASSASLLKKVESFIAKELLLFEYLDGH 175
>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1628
Score = 25.0 bits (52), Expect = 7.5
Identities = 12/46 (26%), Positives = 23/46 (50%)
Frame = -1
Query: 540 LGSKTANRYQIPKHTSAIRDYPSYGNTYCLLYHRPMHSGHAEYAKL 403
+G +++ + ++ +R YP N Y L H + S +A Y+ L
Sbjct: 416 IGDLSSSLLILGRYPFLLRAYPELSNLYHKLLHISISSIYANYSPL 461
>SPBC1271.04c |||deoxyhypusine synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 350
Score = 25.0 bits (52), Expect = 7.5
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 7/50 (14%)
Frame = -2
Query: 314 VGIHAGNANSQSVGICLIGDWRDDLPPEKQ-------LSTTKSLIAQGVQ 186
+G A N + I + +WRD PPE++ L T +LI+ GV+
Sbjct: 55 IGFQATNLHDAVTIINEMRNWRDPNPPEEKSDRATIFLGYTSNLISSGVR 104
>SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/
diacylglycerol ethanolaminesphotranferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 386
Score = 24.6 bits (51), Expect = 10.0
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = -2
Query: 110 LFTYLSTWKHFHPG 69
L+ Y+STW+ +H G
Sbjct: 152 LYFYISTWEEYHTG 165
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 24.6 bits (51), Expect = 10.0
Identities = 7/18 (38%), Positives = 12/18 (66%)
Frame = +2
Query: 401 ESFAYSACPECIGRWYNR 454
+ + + A ECIGRW+ +
Sbjct: 54 DMYGFGANEECIGRWFKQ 71
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 24.6 bits (51), Expect = 10.0
Identities = 9/28 (32%), Positives = 19/28 (67%)
Frame = -3
Query: 535 EQNRQQIPDS*THQCHT*LSIIRQYLLP 452
++ R++I D + CH LS++ ++L+P
Sbjct: 84 KEYRKKIEDELSDICHDVLSVLEKHLIP 111
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,513,297
Number of Sequences: 5004
Number of extensions: 56772
Number of successful extensions: 176
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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