Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_B21
(399 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12900.1 68417.m02018 gamma interferon responsive lysosomal t... 65 2e-11
At4g12870.1 68417.m02015 gamma interferon responsive lysosomal t... 65 2e-11
At4g12890.1 68417.m02017 gamma interferon responsive lysosomal t... 63 6e-11
At4g12960.1 68417.m02025 gamma interferon responsive lysosomal t... 63 8e-11
At1g07080.1 68414.m00754 gamma interferon responsive lysosomal t... 52 1e-07
At5g01580.1 68418.m00073 gamma interferon responsive lysosomal t... 51 2e-07
At3g45810.1 68416.m04958 ferric reductase-like transmembrane com... 29 1.1
At5g61020.2 68418.m07656 YT521-B-like family protein contains Pf... 27 3.5
At5g61020.1 68418.m07655 YT521-B-like family protein contains Pf... 27 3.5
At4g00416.1 68417.m00056 methyl-CpG-binding domain-containing pr... 27 3.5
At3g09070.1 68416.m01066 glycine-rich protein similar to hypothe... 27 6.1
At2g44490.1 68415.m05531 glycosyl hydrolase family 1 protein con... 27 6.1
At2g02720.1 68415.m00214 pectate lyase family protein similar to... 27 6.1
>At4g12900.1 68417.m02018 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 231
Score = 64.9 bits (151), Expect = 2e-11
Identities = 29/76 (38%), Positives = 46/76 (60%), Gaps = 1/76 (1%)
Frame = +1
Query: 4 KVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLVKVKLVPYGKSTHDKVNEKWTFTCHH 180
KVK+ +YYESLCP + FI L ++ D + +KL+P+G + V++ T TC H
Sbjct: 36 KVKLNLYYESLCPSCQNFIVHHLGKIFNTDLHTITDLKLIPFGNA---HVSDDLTVTCQH 92
Query: 181 GPDECYGNKMQACILK 228
G +EC N ++AC ++
Sbjct: 93 GEEECKLNALEACAIR 108
>At4g12870.1 68417.m02015 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 229
Score = 64.9 bits (151), Expect = 2e-11
Identities = 31/75 (41%), Positives = 46/75 (61%), Gaps = 1/75 (1%)
Frame = +1
Query: 4 KVKIAVYYESLCPDSKKFITTQLAPVW-RDFRGLVKVKLVPYGKSTHDKVNEKWTFTCHH 180
KV++ +YYESLCP + FI +L V+ D + VKLVP+G + KV+ T C H
Sbjct: 32 KVELNLYYESLCPGCQSFIVDELVKVFDSDLDTITDVKLVPFG---YAKVSNNLTVICQH 88
Query: 181 GPDECYGNKMQACIL 225
G +EC N ++AC++
Sbjct: 89 GEEECKLNALEACVI 103
>At4g12890.1 68417.m02017 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 232
Score = 63.3 bits (147), Expect = 6e-11
Identities = 34/84 (40%), Positives = 48/84 (57%), Gaps = 4/84 (4%)
Frame = +1
Query: 4 KVKIAVYYESLCPDSKKFITTQLAPVW-RDFRGLVKVKLVPYGKSTHDKVNEKWTFTCHH 180
KVKI +YYESLCP + FI L ++ D + +KLVP+G + ++ T TC H
Sbjct: 38 KVKINLYYESLCPYCQNFIVDDLGKIFDSDLLKITDLKLVPFGNA---HISNNLTITCQH 94
Query: 181 GPDECYGNKMQAC---ILKDRRLQ 243
G +EC N ++AC L D +LQ
Sbjct: 95 GEEECKLNALEACGIRTLPDPKLQ 118
>At4g12960.1 68417.m02025 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 62.9 bits (146), Expect = 8e-11
Identities = 29/76 (38%), Positives = 47/76 (61%), Gaps = 1/76 (1%)
Frame = +1
Query: 4 KVKIAVYYESLCPDSKKFITTQLAPVW-RDFRGLVKVKLVPYGKSTHDKVNEKWTFTCHH 180
KVK+ +YYESLCP ++FI L ++ D + +KL P+G + ++++ T TC H
Sbjct: 31 KVKLNLYYESLCPGCQEFIVDDLGKIFDYDLYTITDLKLFPFGNA---ELSDNLTVTCQH 87
Query: 181 GPDECYGNKMQACILK 228
G +EC N ++AC L+
Sbjct: 88 GEEECKLNALEACALR 103
>At1g07080.1 68414.m00754 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 265
Score = 52.4 bits (120), Expect = 1e-07
Identities = 27/75 (36%), Positives = 40/75 (53%), Gaps = 1/75 (1%)
Frame = +1
Query: 4 KVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLVKVKLVPYGKSTHDKVNEKWTFTCHH 180
KV + +YYESLCP FI LA ++ D +V + L P+G + N T C H
Sbjct: 38 KVSVGLYYESLCPYCSSFIVNHLAKLFEDDLISIVDLHLSPWGNTKLRSDNV--TAVCQH 95
Query: 181 GPDECYGNKMQACIL 225
G EC+ + ++AC +
Sbjct: 96 GAFECFLDTVEACAI 110
>At5g01580.1 68418.m00073 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 51.2 bits (117), Expect = 2e-07
Identities = 25/76 (32%), Positives = 41/76 (53%), Gaps = 1/76 (1%)
Frame = +1
Query: 1 QKVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLVKVKLVPYGKSTHDKVNEKWTFTCH 177
QKV +++YYE+LCP +FI +L ++ + ++LVP+G + + T C
Sbjct: 25 QKVTLSLYYEALCPFCAEFIVNRLPKIFETGLISSIDLQLVPWGNAA---IRPDGTILCQ 81
Query: 178 HGPDECYGNKMQACIL 225
HG EC N + AC +
Sbjct: 82 HGEAECALNAIHACAI 97
>At3g45810.1 68416.m04958 ferric reductase-like transmembrane
component family protein similar to respiratory burst
oxidase protein D RbohD from Arabidopsis thaliana,
EMBL:AF055357 [gi:3242789], similar to respiratory burst
oxidase protein D RbohD from Arabidopsis thaliana,
EMBL:AF055357 [gi:3242789], respiratory burst oxidase
homolog from Solanum tuberosum [GI:16549089]; contains
Pfam profile PF01794 Ferric reductase like transmembrane
component
Length = 912
Score = 29.1 bits (62), Expect = 1.1
Identities = 22/88 (25%), Positives = 37/88 (42%), Gaps = 1/88 (1%)
Frame = +2
Query: 47 VRSSSRHSWRPSGGTSEDSSRLSWCLMERARMTR*MKSGLSRAITDLTNATE-IRCKHAS 223
+RS S + WR SG S+R S L +++ + G R ++A ++
Sbjct: 65 IRSMSVNKWRKSGNLGSPSTRKSGNLGPPLPVSQVKRPGPQRVERTTSSAARGLQSLRFL 124
Query: 224 SRTAGSRIRRRWNWLSDMMSQASPDQSL 307
RT R R W + + +Q + D L
Sbjct: 125 DRTVTGRERDSWRSIENRFNQFAVDGRL 152
>At5g61020.2 68418.m07656 YT521-B-like family protein contains Pfam
profile PF04146: YT521-B-like family
Length = 493
Score = 27.5 bits (58), Expect = 3.5
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = +1
Query: 124 YGKSTHDKVNEKWTFTCHHGPDECYGNKMQACILKDRRLQDTEKM 258
YGK + +NE G + G+K+ A LK++R+ +TEK+
Sbjct: 191 YGKENIEGLNEMNRGPRAKGFNSQDGSKVMAVSLKEQRVTETEKL 235
>At5g61020.1 68418.m07655 YT521-B-like family protein contains Pfam
profile PF04146: YT521-B-like family
Length = 495
Score = 27.5 bits (58), Expect = 3.5
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = +1
Query: 124 YGKSTHDKVNEKWTFTCHHGPDECYGNKMQACILKDRRLQDTEKM 258
YGK + +NE G + G+K+ A LK++R+ +TEK+
Sbjct: 193 YGKENIEGLNEMNRGPRAKGFNSQDGSKVMAVSLKEQRVTETEKL 237
>At4g00416.1 68417.m00056 methyl-CpG-binding domain-containing
protein contains Pfam profile PF01429: Methyl-CpG
binding domain
Length = 163
Score = 27.5 bits (58), Expect = 3.5
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +1
Query: 7 VKIAVYYESLCPDSKKF 57
VK+ VYYESL P K+F
Sbjct: 90 VKVDVYYESLAPRRKRF 106
>At3g09070.1 68416.m01066 glycine-rich protein similar to
hypothetical protein GB:AAD32765 [Arabidopsis thaliana]
Length = 685
Score = 26.6 bits (56), Expect = 6.1
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = +2
Query: 41 RTVRSSSRHSWRPSGGTS 94
R VRS+S SWR SGG S
Sbjct: 599 RMVRSNSNVSWRSSGGGS 616
>At2g44490.1 68415.m05531 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
similar to anther-specific protein ATA27 (GI:2746341)
[Arabidopsis thaliana]
Length = 560
Score = 26.6 bits (56), Expect = 6.1
Identities = 16/50 (32%), Positives = 23/50 (46%)
Frame = +1
Query: 22 YYESLCPDSKKFITTQLAPVWRDFRGLVKVKLVPYGKSTHDKVNEKWTFT 171
YY SL S K + P WR +G+ +K GK + +W+FT
Sbjct: 325 YYSSLFVKSIKHVDPT-QPTWRTDQGVDWMKTNIDGKQIAKQGGSEWSFT 373
>At2g02720.1 68415.m00214 pectate lyase family protein similar to
pectate lyase P59 SP:P15722 from [Lycopersicon
esculentum]
Length = 455
Score = 26.6 bits (56), Expect = 6.1
Identities = 16/63 (25%), Positives = 30/63 (47%)
Frame = +2
Query: 71 WRPSGGTSEDSSRLSWCLMERARMTR*MKSGLSRAITDLTNATEIRCKHASSRTAGSRIR 250
WR ++ +L+ C++ R T K+G +TD ++ ++ K + R A +R R
Sbjct: 107 WRGDKNWDKNRKKLADCVLGFGRKTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDR 166
Query: 251 RRW 259
W
Sbjct: 167 PLW 169
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,302,346
Number of Sequences: 28952
Number of extensions: 185991
Number of successful extensions: 524
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 513
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 518
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 575830496
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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