BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_B09
(469 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein L22|Schizosa... 62 5e-11
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 29 0.47
SPCC1753.03c |rec7||meiotic recombination protein Rec7 |Schizosa... 27 1.4
SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 26 3.3
SPAC1399.04c |||uracil phosphoribosyltransferase |Schizosaccharo... 25 4.4
SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 7.6
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 25 7.6
>SPAC11E3.15 |rpl22|SPAP8A3.01|60S ribosomal protein
L22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 117
Score = 61.7 bits (143), Expect = 5e-11
Identities = 29/58 (50%), Positives = 41/58 (70%), Gaps = 1/58 (1%)
Frame = +1
Query: 130 ISLKFTIDCTHPAEDSILDVGNFEKYLKERVKVEGKTNNLGNHVVIARD-KTKVAINA 300
+S K+ ID T D I DV FEKYL +R+KV+GKT NLG+ VV++R+ +K+A+ A
Sbjct: 8 VSNKYIIDATAAVNDKIFDVAAFEKYLIDRIKVDGKTGNLGSSVVVSREGSSKIAVIA 65
>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1064
Score = 28.7 bits (61), Expect = 0.47
Identities = 19/62 (30%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
Frame = -3
Query: 260 T*LPRLFVLPSTLTRSFKYFSKLPTSRMLSSAGCVQSMVNLRLIF-LLAPLPRILPPFTP 84
T +P+L L +T + F++ R+ G ++++ L ++F +L L RI+ PFTP
Sbjct: 714 TVVPQLLGLIEEMTNWYIRFNR---RRLKGEDGEIETINALNVLFEVLFTLVRIMGPFTP 770
Query: 83 FL 78
F+
Sbjct: 771 FI 772
>SPCC1753.03c |rec7||meiotic recombination protein Rec7
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 339
Score = 27.1 bits (57), Expect = 1.4
Identities = 10/42 (23%), Positives = 25/42 (59%)
Frame = +3
Query: 318 VTWSNFSYQARTVKIGTSILEISRNKHTAKKIKIVFLVHSLL 443
+ WS++S T+ + + +L+I +++ + I ++ L H L+
Sbjct: 23 INWSHYSDGGFTLSLTSGLLQIRQHEELIQSINLLDLWHQLI 64
>SPBC354.13 |rga6||GTPase activating protein
Rga6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 733
Score = 25.8 bits (54), Expect = 3.3
Identities = 15/64 (23%), Positives = 29/64 (45%)
Frame = +1
Query: 166 AEDSILDVGNFEKYLKERVKVEGKTNNLGNHVVIARDKTKVAINAD*KLPKLRGVTSAIR 345
+E+S +V + K + ++ N N +A + TKV ++ PK+ V+
Sbjct: 590 SEESYPNVKHISKLPLINDSSDNESGNQENDDAVANESTKVVVDNQQPQPKISTVSDTAV 649
Query: 346 PELS 357
P +S
Sbjct: 650 PSMS 653
>SPAC1399.04c |||uracil phosphoribosyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 220
Score = 25.4 bits (53), Expect = 4.4
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +1
Query: 307 KLPKLRGVTSAIRPELS 357
K PKLR VT++I PEL+
Sbjct: 184 KFPKLRMVTASIDPELN 200
>SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 879
Score = 24.6 bits (51), Expect = 7.6
Identities = 18/59 (30%), Positives = 30/59 (50%), Gaps = 3/59 (5%)
Frame = +1
Query: 202 KYLKERVKVEGKTNNLGNHVVIARDKTKVAINAD*KLPKLRGVT---SAIRPELSKLVH 369
K LK+++ G + G+H V R + +N K+ L G+ S +RP++ K VH
Sbjct: 95 KDLKDKLFYVGFRGSFGDHHVNPRTLRAMHLN---KMISLEGIVTRCSFVRPKVIKSVH 150
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 24.6 bits (51), Expect = 7.6
Identities = 9/25 (36%), Positives = 15/25 (60%)
Frame = +1
Query: 184 DVGNFEKYLKERVKVEGKTNNLGNH 258
+ GN +KY + +KV GK ++ H
Sbjct: 955 EAGNLKKYDQPNLKVSGKNDSFVTH 979
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,937,458
Number of Sequences: 5004
Number of extensions: 37217
Number of successful extensions: 106
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 105
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 106
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 178394480
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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