Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_A15
(643 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom... 216 3e-57
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom... 158 7e-40
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 151 8e-38
SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le... 91 2e-19
SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 40 4e-04
SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyce... 34 0.015
SPAC13F5.06c |sec10||exocyst complex subunit Sec10|Schizosacchar... 27 2.3
SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 27 2.3
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 27 3.0
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 26 5.3
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 25 7.0
SPCC553.07c |mug40||DinB translesion DNA repair polymerase|Schiz... 25 7.0
SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyc... 25 9.3
>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 216 bits (527), Expect = 3e-57
Identities = 100/167 (59%), Positives = 130/167 (77%), Gaps = 3/167 (1%)
Frame = +3
Query: 6 VHKANIMRMSDGLFLRCCRDLATKYSDIKFEERYLDTVCLNMVQDPSKFD--VLVMPNLY 179
VHKA IMRM+DGLFL C ++LA +Y DI+ E LD CL +V DP ++ V+VMPNLY
Sbjct: 211 VHKATIMRMADGLFLECAKELAPEYPDIELREEILDNACLKIVTDPVPYNNTVMVMPNLY 270
Query: 180 GDIMSDMCSGLVGGLGLTPSGNIGKNGALFESVHGTAPDIAGKDMANPTALLLSAIMMLR 359
GDI+SDMC+GL+GGLGLTPSGNIG ++FE+VHGTAPDIAGK +ANPTALLLS++MML+
Sbjct: 271 GDIVSDMCAGLIGGLGLTPSGNIGNQASIFEAVHGTAPDIAGKGLANPTALLLSSVMMLK 330
Query: 360 HLQLNEHADRVQNACYEVLREG-KSLTGDLGGTGKCSEYTNAIISKL 497
H+ LN++A R+++A ++ L + T DLGG +YT+AIISKL
Sbjct: 331 HMNLNDYAKRIESAIFDTLANNPDARTKDLGGKSNNVQYTDAIISKL 377
>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 158 bits (383), Expect = 7e-40
Identities = 77/165 (46%), Positives = 108/165 (65%), Gaps = 1/165 (0%)
Frame = +3
Query: 6 VHKANIMRMSDGLFLRCCRDLATKYSDIKFEERYLDTVCLNMVQDPSKFDVLVMPNLYGD 185
+HKANIM+++DGLF R D+A Y I ++ +D + V P +FDVLVMPNLYG
Sbjct: 189 IHKANIMKLADGLFRRTFYDVANGYDAITPKDLIVDNASMQAVSRPQQFDVLVMPNLYGS 248
Query: 186 IMSDMCSGLVGGLGLTPSGNIGKNGALFE-SVHGTAPDIAGKDMANPTALLLSAIMMLRH 362
I+S++ S LVGG G+ P N G++ ALFE I G+ ANPTA +LSA +MLRH
Sbjct: 249 ILSNIGSALVGGPGVIPGANFGRDYALFEPGCRHVGLSITGRGEANPTAAILSACLMLRH 308
Query: 363 LQLNEHADRVQNACYEVLREGKSLTGDLGGTGKCSEYTNAIISKL 497
L L ++AD + A Y V+ EGK+LT DLGG+ ++T+AI+ ++
Sbjct: 309 LGLKDYADLINAATYSVIEEGKTLTKDLGGSASTGDFTHAILERM 353
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 151 bits (366), Expect = 8e-38
Identities = 72/168 (42%), Positives = 107/168 (63%), Gaps = 3/168 (1%)
Frame = +3
Query: 6 VHKANIMRMSDGLFLRCCR---DLATKYSDIKFEERYLDTVCLNMVQDPSKFDVLVMPNL 176
+HK+N+M ++DGLF CR L Y+ I +E+ +D++ + ++P FDV+V PNL
Sbjct: 194 IHKSNVMSVTDGLFRESCRHAQSLDPSYASINVDEQIVDSMVYRLFREPECFDVVVAPNL 253
Query: 177 YGDIMSDMCSGLVGGLGLTPSGNIGKNGALFESVHGTAPDIAGKDMANPTALLLSAIMML 356
YGDI+SD + L+G LGL PS N+G N + E VHG+APDIAG+ +ANP A S +ML
Sbjct: 254 YGDILSDGAASLIGSLGLVPSANVGDNFVMSEPVHGSAPDIAGRGIANPVATFRSVALML 313
Query: 357 RHLQLNEHADRVQNACYEVLREGKSLTGDLGGTGKCSEYTNAIISKLN 500
+ + A + A +VL EGK LT DLGG +E T+A+++ ++
Sbjct: 314 EFMGHQDAAADIYTAVDKVLTEGKVLTPDLGGKSGTNEITDAVLANIH 361
>SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase
Leu1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 371
Score = 90.6 bits (215), Expect = 2e-19
Identities = 59/177 (33%), Positives = 98/177 (55%), Gaps = 15/177 (8%)
Frame = +3
Query: 12 KANIMRMSDGLFLRCCRDLATKYSDIKFEERYLDTVCLNMVQDPSKFD-VLVMPNLYGDI 188
KAN++ S + +Y + + + +D+ + +V+ P + V++ NL+GDI
Sbjct: 191 KANVLATSRLWRKTVAKIFKEEYPHLTLKNQLIDSAAMLLVKSPRTLNGVVLTDNLFGDI 250
Query: 189 MSDMCSGLVGGLGLTPS----GNIGKNG----ALFESVHGTAPDIAGKDMANPTALLLSA 344
+SD S + G LGL PS G +GK+ L E +HG+APDIAGK + NP +LSA
Sbjct: 251 ISDEASVIPGSLGLLPSASLSGVVGKSEEKVHCLVEPIHGSAPDIAGKGIVNPVGTILSA 310
Query: 345 IMMLRH-LQLNEHADRVQNACYEVLRE----GKSL-TGDLGGTGKCSEYTNAIISKL 497
++LR+ L + A+ ++ A +VL + G+ L T DLGG ++ T A++ +L
Sbjct: 311 SLLLRYGLNAPKEAEAIEAAVRKVLDDTSIGGRGLYTRDLGGEASTADITKAVVEEL 367
>SPAC6G10.08 |idp1||isocitrate dehydrogenase
Idp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 418
Score = 39.5 bits (88), Expect = 4e-04
Identities = 33/101 (32%), Positives = 45/101 (44%), Gaps = 9/101 (8%)
Frame = +3
Query: 12 KANIMRMSDGLFLRCCRDLATKYSDIKFEE-------RYLDTVCLNMVQDPSKFDVLVMP 170
K I++ DG F +++ KFEE R +D + ++ F V
Sbjct: 222 KNTILKKYDGRFKDTFQEVYESDYKQKFEELGLWYQHRLIDDMVAQAIKSNGGF-VWACK 280
Query: 171 NLYGDIMSDMCSGLVGGLGLTPSGNIGKNGALFES--VHGT 287
N GD+MSD+ + G LGL S I NG FES HGT
Sbjct: 281 NYDGDVMSDVVAQAYGSLGLMTSVLIHPNGRTFESEAAHGT 321
>SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 342
Score = 34.3 bits (75), Expect = 0.015
Identities = 16/31 (51%), Positives = 17/31 (54%)
Frame = +2
Query: 257 RSPIRVCSRHSSRYRWKGHGKSDRVTSLSYH 349
R+P R SR SS Y W HGK R SL H
Sbjct: 240 RTPSRTLSRSSSHYLWVRHGKLTRSVSLLQH 270
>SPAC13F5.06c |sec10||exocyst complex subunit
Sec10|Schizosaccharomyces pombe|chr 1|||Manual
Length = 811
Score = 27.1 bits (57), Expect = 2.3
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = -3
Query: 614 SHYMLLGIINFMPWHPNFKKVGSIYKNI*DLSIVSYSLIKL*DNCI 477
SH + +F+ WH SI + DLS+V SL+ L D I
Sbjct: 498 SHLNSEKVYSFIGWHAEALNRASILISSQDLSVVISSLVNLLDKLI 543
>SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol
3-phosphatidyltransferase Pis1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 27.1 bits (57), Expect = 2.3
Identities = 9/28 (32%), Positives = 18/28 (64%)
Frame = -3
Query: 608 YMLLGIINFMPWHPNFKKVGSIYKNI*D 525
+++L + FM WHPN+ + +Y ++ D
Sbjct: 39 FLVLISLYFMSWHPNYCTIVYLYSSLLD 66
>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1628
Score = 26.6 bits (56), Expect = 3.0
Identities = 17/54 (31%), Positives = 26/54 (48%)
Frame = -2
Query: 474 CIHYIFQYHRDLQLKIYPRGALHNRRFVHGLHARSTGDVLTS**LREVTRSDLP 313
C Y++ +LK+ P AL+ +FV LH+ T + T L + S LP
Sbjct: 1078 CARYLWTLCIAPRLKMSPHDALYCAKFVKLLHSLGTPNFSTMSFLEILFNSQLP 1131
>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
Prp22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1168
Score = 25.8 bits (54), Expect = 5.3
Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = +3
Query: 21 IMRMSDGLFLRCCR--DLATKYSDIKFEERYLDTVCLNMVQDPSKFDVLVMPNL 176
I M+DG+ R C L +KYS I +E + TV +++ K VL P+L
Sbjct: 604 IKYMTDGMLQRECLVDPLLSKYSVIILDEAHERTVATDVLFGLLKGTVLKRPDL 657
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.4 bits (53), Expect = 7.0
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -2
Query: 333 VTRSDLPCPFQRYRELCREQTRIGLRS 253
+TR+DL + RY E+ + R+GL S
Sbjct: 518 LTRTDLSNIYDRYHEVLSSKHRVGLGS 544
>SPCC553.07c |mug40||DinB translesion DNA repair
polymerase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 547
Score = 25.4 bits (53), Expect = 7.0
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +3
Query: 360 HLQLNEHADRVQNACYEVLREGKSLTGDLGGTGKCSEYTNAIISKL 497
HL+L E A V+N V + K + + G T C N +++K+
Sbjct: 235 HLRLQELAFTVENITMVVEKIRKQVHEETGVTVSCGIAANKLLAKI 280
>SPCC16C4.06c |||tRNA pseudouridylate synthase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 413
Score = 25.0 bits (52), Expect = 9.3
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = -1
Query: 196 SDMMSPYKLGITKTSNLEGSCTMFRQT 116
+ + P++L K L+ +CTMFR T
Sbjct: 204 NSLSEPFRLSKPKLDFLKHACTMFRGT 230
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,834,963
Number of Sequences: 5004
Number of extensions: 60061
Number of successful extensions: 186
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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