Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0002_A14
(460 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) simi... 146 5e-36
At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A) 146 6e-36
At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C) 144 2e-35
At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) simila... 33 0.070
At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) simila... 33 0.12
At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) simila... 33 0.12
At5g63900.1 68418.m08023 PHD finger family protein contains Pfam... 30 0.65
At1g66070.1 68414.m07499 translation initiation factor-related s... 29 1.1
At1g32140.1 68414.m03954 F-box family protein contains F-box dom... 29 1.1
At5g12000.1 68418.m01403 protein kinase family protein contains ... 29 1.5
At4g34630.1 68417.m04918 expressed protein 29 1.5
At1g72440.1 68414.m08377 CCAAT-box-binding transcription factor-... 27 4.6
At3g10900.1 68416.m01312 (1-4)-beta-mannan endohydrolase, putati... 27 8.1
>At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) similar
to 60S RIBOSOMAL PROTEIN L27 GB:P41101 from [Solanum
tuberosum]
Length = 135
Score = 146 bits (355), Expect = 5e-36
Identities = 64/135 (47%), Positives = 94/135 (69%), Gaps = 1/135 (0%)
Frame = +2
Query: 17 MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 196
M K +K K V++L GRYAG+KA+++K++D+GTSD+ YGH VAG+ +YP KV ++
Sbjct: 1 MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAK 60
Query: 197 KIHKRSKIKPFVKGVNYNHLMPTRYSVDFSFEKFSAKD-LKDPAKRKKLRFNTRVRFEER 373
K K+S++K F+K VNY HLMPTRY++D ++ + D LK K+ + + EER
Sbjct: 61 KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEER 120
Query: 374 YKSGKNKWFFQKLRF 418
+K+GKN+WFF KLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135
>At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A)
Length = 135
Score = 146 bits (354), Expect = 6e-36
Identities = 64/135 (47%), Positives = 92/135 (68%), Gaps = 1/135 (0%)
Frame = +2
Query: 17 MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 196
M K MKPGK V++L GRY G+KA++VK++D+GT +K YGH VAG+ +YP KV ++
Sbjct: 1 MVKCMKPGKAVILLQGRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAK 60
Query: 197 KIHKRSKIKPFVKGVNYNHLMPTRYSVDFSFEK-FSAKDLKDPAKRKKLRFNTRVRFEER 373
K K+S++K F K +NY H+MPTRY++D + SA + K+ + +FEER
Sbjct: 61 KTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEER 120
Query: 374 YKSGKNKWFFQKLRF 418
+K+GKN+WFF KLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135
>At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C)
Length = 135
Score = 144 bits (350), Expect = 2e-35
Identities = 62/135 (45%), Positives = 93/135 (68%), Gaps = 1/135 (0%)
Frame = +2
Query: 17 MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 196
M K +K K V++L GRYAG+KA+++K++D+G D+PYGH VAG+ +YP KV ++
Sbjct: 1 MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAK 60
Query: 197 KIHKRSKIKPFVKGVNYNHLMPTRYSVDFSFEKFSAKD-LKDPAKRKKLRFNTRVRFEER 373
K K+S++K F+K VNY HLMPTRY++D ++ + D L+ K+ + + EER
Sbjct: 61 KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEER 120
Query: 374 YKSGKNKWFFQKLRF 418
+K+GKN+WFF KLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135
>At1g18540.1 68414.m02313 60S ribosomal protein L6 (RPL6A) similar
to 60S ribosomal protein L6 GI:7208784 from [Cicer
arietinum]
Length = 233
Score = 33.5 bits (73), Expect = 0.070
Identities = 11/35 (31%), Positives = 23/35 (65%)
Frame = +2
Query: 8 PSKMGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEG 112
P+K+ + PG V+++L+GR+ G++ + +K G
Sbjct: 82 PTKLKASITPGTVLIILAGRFKGKRVVFLKQLSSG 116
>At1g74060.1 68414.m08578 60S ribosomal protein L6 (RPL6B) similar
to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309
from [Cyanophora paradoxa]
Length = 233
Score = 32.7 bits (71), Expect = 0.12
Identities = 11/35 (31%), Positives = 23/35 (65%)
Frame = +2
Query: 8 PSKMGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEG 112
P+K+ + PG V+++L+GR+ G++ + +K G
Sbjct: 82 PAKLRASITPGTVLIILAGRFKGKRVVFLKQLASG 116
>At1g74050.1 68414.m08576 60S ribosomal protein L6 (RPL6C) similar
to 60S ribosomal protein L6 (YL 16 like) GB:CAB57309
from [Cyanophora paradoxa]
Length = 233
Score = 32.7 bits (71), Expect = 0.12
Identities = 11/35 (31%), Positives = 23/35 (65%)
Frame = +2
Query: 8 PSKMGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEG 112
P+K+ + PG V+++L+GR+ G++ + +K G
Sbjct: 82 PTKLRASITPGTVLIILAGRFKGKRVVFLKQLASG 116
>At5g63900.1 68418.m08023 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 557
Score = 30.3 bits (65), Expect = 0.65
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +2
Query: 344 FNTRVRFEERYKSGKNKWFF 403
+N R + E+RYKS K KWF+
Sbjct: 58 YNKRNKKEQRYKSPKGKWFY 77
>At1g66070.1 68414.m07499 translation initiation factor-related
similar to Eukaryotic translation initiation factor 3
subunit 1 (eIF-3 alpha) (eIF3 p35) (eIF3j)
(Swiss-Prot:O75822) [Homo sapiens]
Length = 226
Score = 29.5 bits (63), Expect = 1.1
Identities = 18/60 (30%), Positives = 29/60 (48%)
Frame = +2
Query: 215 KIKPFVKGVNYNHLMPTRYSVDFSFEKFSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNK 394
+IKP+ K +Y L+ T + S A D+KD A N +++ E+ +GK K
Sbjct: 139 RIKPYEKSYHYIALLKT--IMRLSLTNMKAADVKDVASSITTIANEKLKAEKEAAAGKKK 196
>At1g32140.1 68414.m03954 F-box family protein contains F-box domain
Pfam:PF00646
Length = 591
Score = 29.5 bits (63), Expect = 1.1
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = -1
Query: 217 FRPLVNFIFSHPFMNLARVSVDAGDESMSVWFVRC 113
F PL++ FSH F+ R+S G E ++V + RC
Sbjct: 227 FGPLLSLSFSHDFIETGRLSCVKG-EKLAVLYQRC 260
>At5g12000.1 68418.m01403 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 703
Score = 29.1 bits (62), Expect = 1.5
Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = +2
Query: 155 DRYPRKVHKRMGKNKIHKR-SKIKPFVKGVNYNHLMPTRYSVDFSFEKFSAKDLKDPAKR 331
DR PR +R G+N + +R S K V H +PT S+DF++E K ++ R
Sbjct: 188 DRSPRS--QRNGRNTVPERYSHENKGFKPVREMHKIPTNGSLDFNYEFRQGKGQRNSTGR 245
>At4g34630.1 68417.m04918 expressed protein
Length = 199
Score = 29.1 bits (62), Expect = 1.5
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +2
Query: 104 DEGTSDKPYGHAFVAGIDRYPRKVHKRMGKNKIHKRSK 217
D+G D+ Y ++ + PR V K++G+ ++ K K
Sbjct: 130 DDGEEDREYDDSYDLDEELVPRSVSKKVGRQRMRKLGK 167
>At1g72440.1 68414.m08377 CCAAT-box-binding transcription
factor-related similar to CCAAT-box-binding transcription
factor (CCAAT-binding factor) (CBF) (Swiss-Prot:Q03701)
[Homo sapiens], GB:P53569 [Mus musculus]
Length = 1056
Score = 27.5 bits (58), Expect = 4.6
Identities = 17/52 (32%), Positives = 21/52 (40%)
Frame = +2
Query: 182 RMGKNKIHKRSKIKPFVKGVNYNHLMPTRYSVDFSFEKFSAKDLKDPAKRKK 337
R K K KR + PF Y HL+ D K K +P K+KK
Sbjct: 1000 RSKKKKKEKRKRKSPFASLEEYKHLIDQDEKED---SKTKRKATSEPTKKKK 1048
>At3g10900.1 68416.m01312 (1-4)-beta-mannan endohydrolase, putative
similar to (1-4)-beta-mannan endohydrolase [Coffea
arabica] GI:10178872, (1-4)-beta-mannan endohydrolase
GB:AAB87859 [Lycopersicon esculentum]; contains Pfam
profile PF00150: Cellulase (glycosyl hydrolase family 5)
Length = 408
Score = 26.6 bits (56), Expect = 8.1
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +2
Query: 164 PRKVHKRMGKNKIHKRSKIKPFVKGVNYNHLMPT 265
PR + GK + +++ PFVK V+ NHL+ T
Sbjct: 203 PRCGVDKSGKTLMDWINEMAPFVKSVDPNHLLST 236
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,455,352
Number of Sequences: 28952
Number of extensions: 214695
Number of successful extensions: 586
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 581
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 583
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 762235320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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