Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0002_A12
         (445 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g60930.1 68418.m07643 chromosome-associated kinesin, putative...    29   1.1  
At2g42890.2 68415.m05312 RNA recognition motif (RRM)-containing ...    29   1.1  
At2g42890.1 68415.m05311 RNA recognition motif (RRM)-containing ...    29   1.1  
At5g50115.1 68418.m06206 hypothetical protein temporary automate...    28   3.3  
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p...    28   3.3  
At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein si...    27   4.3  
At5g01260.2 68418.m00035 glycoside hydrolase starch-binding doma...    27   5.7  
At5g01260.1 68418.m00034 glycoside hydrolase starch-binding doma...    27   5.7  
At3g15820.1 68416.m02002 phosphatidic acid phosphatase-related /...    27   5.7  
At2g29700.1 68415.m03610 pleckstrin homology (PH) domain-contain...    27   5.7  
At4g25730.1 68417.m03703 FtsJ-like methyltransferase family prot...    27   7.5  
At1g21170.1 68414.m02647 expressed protein                             26   10.0 
At1g08680.2 68414.m00965 ARF GAP-like zinc finger-containing pro...    26   10.0 
At1g08680.1 68414.m00964 ARF GAP-like zinc finger-containing pro...    26   10.0 

>At5g60930.1 68418.m07643 chromosome-associated kinesin, putative
            microtubule-associated motor KIF4 , Mus musculus,
            PIR:A54803
          Length = 1294

 Score = 29.5 bits (63), Expect = 1.1
 Identities = 21/57 (36%), Positives = 30/57 (52%), Gaps = 5/57 (8%)
 Frame = +3

Query: 195  GLPRGDISESEHLENG----MADSLDERRFQEAERARVSEIL-NESRDAPDDEINLD 350
            G     ISESE LENG     +D  D+ + Q+   +R + +L N   D P++E N D
Sbjct: 1100 GKENNSISESEALENGENSQESDEKDKGQQQQVLASRGAMLLQNALADKPEEETNDD 1156


>At2g42890.2 68415.m05312 RNA recognition motif (RRM)-containing
           protein
          Length = 830

 Score = 29.5 bits (63), Expect = 1.1
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = +1

Query: 136 NIVNAIGAYVRRLPSGRRPRGFPAGTSPSRSTSR 237
           N  N +G++V   P G  P G P   SPS + +R
Sbjct: 365 NFYNQVGSHVANSPPGNWPIGSPVKGSPSHAFTR 398


>At2g42890.1 68415.m05311 RNA recognition motif (RRM)-containing
           protein
          Length = 843

 Score = 29.5 bits (63), Expect = 1.1
 Identities = 13/34 (38%), Positives = 18/34 (52%)
 Frame = +1

Query: 136 NIVNAIGAYVRRLPSGRRPRGFPAGTSPSRSTSR 237
           N  N +G++V   P G  P G P   SPS + +R
Sbjct: 378 NFYNQVGSHVANSPPGNWPIGSPVKGSPSHAFTR 411


>At5g50115.1 68418.m06206 hypothetical protein temporary automated
           functional assignment
          Length = 485

 Score = 27.9 bits (59), Expect = 3.3
 Identities = 10/34 (29%), Positives = 21/34 (61%)
 Frame = +3

Query: 246 ADSLDERRFQEAERARVSEILNESRDAPDDEINL 347
           + SLD +++  A+R +++E  N +    +DE N+
Sbjct: 231 SSSLDSKKYDAAKRVKINETKNTTHMVNEDEYNV 264


>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
           protein contains Pfam profile PF01480: PWI domain
          Length = 878

 Score = 27.9 bits (59), Expect = 3.3
 Identities = 19/58 (32%), Positives = 23/58 (39%), Gaps = 7/58 (12%)
 Frame = -2

Query: 333 RQEHRVTHSG-------SRRHELSQPPGIVSRPGYPPCRSRGAPTRRCPRGEAPRTSA 181
           R+  R TH G       SRR     PP    R   PP R R +P+    R  +P   A
Sbjct: 290 RRHRRPTHEGRRQSPAPSRRRRSPSPPARRRRSPSPPARRRRSPSPPARRHRSPTPPA 347


>At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein
           similar to lysosomal alpha-mannosidase GI:3522867 from
           [Homo sapiens]
          Length = 1019

 Score = 27.5 bits (58), Expect = 4.3
 Identities = 14/34 (41%), Positives = 19/34 (55%)
 Frame = +3

Query: 231 LENGMADSLDERRFQEAERARVSEILNESRDAPD 332
           LENG  + +  RR Q  +   V EILNE+   P+
Sbjct: 794 LENGQIELMLHRRMQHDDIRGVGEILNETVCLPE 827


>At5g01260.2 68418.m00035 glycoside hydrolase starch-binding
           domain-containing protein low similarity to SP|P31797
           Cyclomaltodextrin glucanotransferase precursor (EC
           2.4.1.19) (Cyclodextrin-glycosyltransferase) (CGTase)
           {Bacillus stearothermophilus}; contains Pfam profile
           PF00686: Starch binding domain
          Length = 385

 Score = 27.1 bits (57), Expect = 5.7
 Identities = 15/60 (25%), Positives = 25/60 (41%)
 Frame = +3

Query: 228 HLENGMADSLDERRFQEAERARVSEILNESRDAPDDEINLDDPCMKVHCSARRVC*INEH 407
           H++    DS    R  +    R S I +   +  D+EI   +  ++V    R+ C   EH
Sbjct: 43  HIKFLRLDSAQSSRILKPVPLRSSSIKDSQVNVEDEEIEASNKTVRVRFQLRKECVFGEH 102


>At5g01260.1 68418.m00034 glycoside hydrolase starch-binding
           domain-containing protein low similarity to SP|P31797
           Cyclomaltodextrin glucanotransferase precursor (EC
           2.4.1.19) (Cyclodextrin-glycosyltransferase) (CGTase)
           {Bacillus stearothermophilus}; contains Pfam profile
           PF00686: Starch binding domain
          Length = 306

 Score = 27.1 bits (57), Expect = 5.7
 Identities = 15/60 (25%), Positives = 25/60 (41%)
 Frame = +3

Query: 228 HLENGMADSLDERRFQEAERARVSEILNESRDAPDDEINLDDPCMKVHCSARRVC*INEH 407
           H++    DS    R  +    R S I +   +  D+EI   +  ++V    R+ C   EH
Sbjct: 43  HIKFLRLDSAQSSRILKPVPLRSSSIKDSQVNVEDEEIEASNKTVRVRFQLRKECVFGEH 102


>At3g15820.1 68416.m02002 phosphatidic acid phosphatase-related /
           PAP2-related contains Pfam profile PF01569: PAP2
           superfamily
          Length = 301

 Score = 27.1 bits (57), Expect = 5.7
 Identities = 14/30 (46%), Positives = 16/30 (53%)
 Frame = +3

Query: 222 SEHLENGMADSLDERRFQEAERARVSEILN 311
           S H+   M  SLD RR Q    A V +ILN
Sbjct: 214 SGHVAGSMIASLDMRRMQRLRLAMVFDILN 243


>At2g29700.1 68415.m03610 pleckstrin homology (PH) domain-containing
           protein (PH1) identical to AtPH1 [Arabidopsis thaliana]
           GI:5926716; contains Pfam profile PF00169: PH domain
          Length = 145

 Score = 27.1 bits (57), Expect = 5.7
 Identities = 11/23 (47%), Positives = 16/23 (69%)
 Frame = +2

Query: 158 LTYDDYRAADVRGASPRGHLRVG 226
           L + D  AA +RG++PRG + VG
Sbjct: 56  LWFKDQAAAGIRGSTPRGVISVG 78


>At4g25730.1 68417.m03703 FtsJ-like methyltransferase family protein
           contains Pfam profile: PF01728 FtsJ-like
           methyltransferase
          Length = 821

 Score = 26.6 bits (56), Expect = 7.5
 Identities = 10/13 (76%), Positives = 11/13 (84%)
 Frame = -3

Query: 218 GDVPAGKPRGRRP 180
           G  PAGKPRGR+P
Sbjct: 808 GKRPAGKPRGRKP 820


>At1g21170.1 68414.m02647 expressed protein
          Length = 1090

 Score = 26.2 bits (55), Expect = 10.0
 Identities = 14/29 (48%), Positives = 16/29 (55%)
 Frame = +3

Query: 264 RRFQEAERARVSEILNESRDAPDDEINLD 350
           +R  EAE AR    + ESR AP    NLD
Sbjct: 134 KRVNEAELARKVRDMRESRTAPSVTQNLD 162


>At1g08680.2 68414.m00965 ARF GAP-like zinc finger-containing
           protein ZiGA4 (ZIGA4) nearly identical to ARF GAP-like
           zinc finger-containing protein ZiGA4 GI:10441354 from
           [Arabidopsis thaliana]; contains InterPro accession
           IPR001164: Human Rev interacting-like protein (hRIP)
          Length = 648

 Score = 26.2 bits (55), Expect = 10.0
 Identities = 11/32 (34%), Positives = 15/32 (46%)
 Frame = +1

Query: 154 GAYVRRLPSGRRPRGFPAGTSPSRSTSRTAWR 249
           G    R+P+G    GFP     + S S  AW+
Sbjct: 519 GTQTTRIPAGSSAFGFPGNIGMAPSYSEEAWQ 550


>At1g08680.1 68414.m00964 ARF GAP-like zinc finger-containing
           protein ZiGA4 (ZIGA4) nearly identical to ARF GAP-like
           zinc finger-containing protein ZiGA4 GI:10441354 from
           [Arabidopsis thaliana]; contains InterPro accession
           IPR001164: Human Rev interacting-like protein (hRIP)
          Length = 649

 Score = 26.2 bits (55), Expect = 10.0
 Identities = 11/32 (34%), Positives = 15/32 (46%)
 Frame = +1

Query: 154 GAYVRRLPSGRRPRGFPAGTSPSRSTSRTAWR 249
           G    R+P+G    GFP     + S S  AW+
Sbjct: 520 GTQTTRIPAGSSAFGFPGNIGMAPSYSEEAWQ 551


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,315,769
Number of Sequences: 28952
Number of extensions: 179951
Number of successful extensions: 604
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 576
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 603
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 712739520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)