Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0002_A04
         (443 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g05830.1 68415.m00630 eukaryotic translation initiation facto...    73   7e-14
At2g05830.2 68415.m00631 eukaryotic translation initiation facto...    51   4e-07
At1g20575.1 68414.m02567 dolichyl-phosphate beta-D-mannosyltrans...    29   1.4  
At5g20960.2 68418.m02492 aldehyde oxidase 1 (AAO1) identical to ...    27   5.7  
At5g20960.1 68418.m02491 aldehyde oxidase 1 (AAO1) identical to ...    27   5.7  

>At2g05830.1 68415.m00630 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           CIG2 [Nicotiana tabacum] GI:15216226, IDI2 [Hordeum
           vulgare subsp. vulgare] GI:12407304; weak similarity to
           SP|Q64270 Translation initiation factor eIF-2B alpha
           subunit {Rattus norvegicus}; contains Pfam profile
           PF01008: Initiation factor 2 subunit family
          Length = 374

 Score = 73.3 bits (172), Expect = 7e-14
 Identities = 40/96 (41%), Positives = 57/96 (59%), Gaps = 2/96 (2%)
 Frame = +3

Query: 78  SLESIKYRRGSXXXXXXXXXXXQTRYVKVQGVEDGWRVINKMQVRGAPAIAIVGCLSLAI 257
           +L++I Y+ GS           +T Y++++   DGW  I +M VRGAPAIAI   LSLA+
Sbjct: 8   TLKAICYKPGSLQLLDQRKLPLETIYLEIRDASDGWSAIQEMVVRGAPAIAIAAALSLAV 67

Query: 258 ELLKDNIADKKIMRQE--IEGKLNYLVSARPTAVNI 359
           E+   +  D         +E KL+YLVS+RPTAVN+
Sbjct: 68  EVFNFHGFDGSASDAVAFLENKLDYLVSSRPTAVNL 103


>At2g05830.2 68415.m00631 eukaryotic translation initiation factor
           2B family protein / eIF-2B family protein similar to
           CIG2 [Nicotiana tabacum] GI:15216226, IDI2 [Hordeum
           vulgare subsp. vulgare] GI:12407304; weak similarity to
           SP|Q64270 Translation initiation factor eIF-2B alpha
           subunit {Rattus norvegicus}; contains Pfam profile
           PF01008: Initiation factor 2 subunit family
          Length = 326

 Score = 50.8 bits (116), Expect = 4e-07
 Identities = 29/55 (52%), Positives = 36/55 (65%), Gaps = 2/55 (3%)
 Frame = +3

Query: 201 MQVRGAPAIAIVGCLSLAIELLKDNIADKKIMRQE--IEGKLNYLVSARPTAVNI 359
           M VRGAPAIAI   LSLA+E+   +  D         +E KL+YLVS+RPTAVN+
Sbjct: 1   MVVRGAPAIAIAAALSLAVEVFNFHGFDGSASDAVAFLENKLDYLVSSRPTAVNL 55


>At1g20575.1 68414.m02567 dolichyl-phosphate
           beta-D-mannosyltransferase, putative /
           dolichol-phosphate mannosyltransferase, putative /
           mannose-P-dolichol synthase, putative similar to DPM1
           from Homo sapiens [SP|O60762]; member of
           glycosyltransferase family 2
          Length = 246

 Score = 29.1 bits (62), Expect = 1.4
 Identities = 14/28 (50%), Positives = 18/28 (64%)
 Frame = +3

Query: 147 TRYVKVQGVEDGWRVINKMQVRGAPAIA 230
           TRYVK  GV  GW ++ K+  RGA  +A
Sbjct: 132 TRYVKGGGVH-GWNLMRKLTSRGANVLA 158


>At5g20960.2 68418.m02492 aldehyde oxidase 1 (AAO1) identical to
            aldehyde oxidase AAO1 from Arabidopsis thaliana
            [gi:3172023] isoform contains a GA-donor splice site at
            intron 10
          Length = 1368

 Score = 27.1 bits (57), Expect = 5.7
 Identities = 13/38 (34%), Positives = 24/38 (63%)
 Frame = +3

Query: 156  VKVQGVEDGWRVINKMQVRGAPAIAIVGCLSLAIELLK 269
            V+VQG+E G  +  K++   A ++ ++ C + + ELLK
Sbjct: 1063 VEVQGIEIGQGLWTKVKQMAAYSLGLIQCGTTSDELLK 1100


>At5g20960.1 68418.m02491 aldehyde oxidase 1 (AAO1) identical to
            aldehyde oxidase AAO1 from Arabidopsis thaliana
            [gi:3172023] isoform contains a GA-donor splice site at
            intron 10
          Length = 1368

 Score = 27.1 bits (57), Expect = 5.7
 Identities = 13/38 (34%), Positives = 24/38 (63%)
 Frame = +3

Query: 156  VKVQGVEDGWRVINKMQVRGAPAIAIVGCLSLAIELLK 269
            V+VQG+E G  +  K++   A ++ ++ C + + ELLK
Sbjct: 1063 VEVQGIEIGQGLWTKVKQMAAYSLGLIQCGTTSDELLK 1100


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,009,743
Number of Sequences: 28952
Number of extensions: 137282
Number of successful extensions: 352
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 346
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 350
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 712739520
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)