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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0001_O18
         (510 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    85   3e-17
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    72   2e-13
At2g18720.1 68415.m02180 eukaryotic translation initiation facto...    30   1.0  
At1g34720.1 68414.m04316 hypothetical protein                          28   3.2  
At4g05300.1 68417.m00803 hypothetical protein                          27   5.5  
At3g43380.1 68416.m04591 hypothetical protein                          27   5.5  
At3g24370.1 68416.m03060 hypothetical protein                          27   5.5  
At1g27800.1 68414.m03403 hypothetical protein                          27   5.5  
At5g52550.1 68418.m06525 expressed protein                             27   7.3  
At3g43680.1 68416.m04657 hypothetical protein                          27   7.3  
At3g58610.1 68416.m06532 ketol-acid reductoisomerase identical t...    27   9.7  

>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|Q13144
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
           contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 85.0 bits (201), Expect = 3e-17
 Identities = 48/160 (30%), Positives = 83/160 (51%), Gaps = 3/160 (1%)
 Frame = +2

Query: 2   LKGFEPQQRIKLARMTALWIG---NGCVPPSVLLVLVNEHLLKDNLALEFVLEVFATVKQ 172
           L+ FE  +R KLA  TAL      +G  P +V   L+ ++L+   + L FV + F     
Sbjct: 135 LELFEENERKKLAIFTALAFSQKLSGLPPETVFQPLLKDNLVAKGIVLSFVTDFFKEYLV 194

Query: 173 ERGVASLIAALKRGQLEGRLLEFLPLNRRSEDVLATSFASRGLAELLRLHRAQASQEARR 352
           E  +  LI+ L+RG++E  L++FLP  RRS +  A  F + GL +L+  H  +  +   R
Sbjct: 195 ENSLEDLISILRRGKMEDNLMDFLPPVRRSAESFAEHFTNEGLTDLVEYHSKKMFEVKLR 254

Query: 353 ELTQALLDELAEEKPVRDLIQDLRDMALKHSIPEHETIAI 472
           E+   L  ++ EE  V ++I+ ++       +P+ E + +
Sbjct: 255 EIKTVLTSKVTEESNVDEVIESVKQQIKDAKLPDIEVVRV 294


>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|P47823
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Oryctolagus
           cuniculus}; contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 72.1 bits (169), Expect = 2e-13
 Identities = 44/160 (27%), Positives = 79/160 (49%), Gaps = 3/160 (1%)
 Frame = +2

Query: 2   LKGFEPQQRIKLARMTALWIG---NGCVPPSVLLVLVNEHLLKDNLALEFVLEVFATVKQ 172
           L+ FE  +R KLA  TAL      +G    +V   L+ + L+   + L FV + F     
Sbjct: 135 LELFEENERKKLAIFTALAFSQKLSGLPAETVFQPLLKDTLVAKGIVLNFVTDFFNEYLV 194

Query: 173 ERGVASLIAALKRGQLEGRLLEFLPLNRRSEDVLATSFASRGLAELLRLHRAQASQEARR 352
           E  +  LI+ L+RG+++ +LLEFLP  +R+ +  A  F   GL  L+  +  +  +   +
Sbjct: 195 ENSLDDLISILRRGKMDDKLLEFLPPTKRTTESFAEHFTKAGLTALVEYNERKIFEVKLK 254

Query: 353 ELTQALLDELAEEKPVRDLIQDLRDMALKHSIPEHETIAI 472
           E+   L  ++ EE  V ++ + ++       +PE E + +
Sbjct: 255 EIKAVLTSQVTEEINVDEVTEMVKQQVKDAKLPETEVVHV 294


>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
           subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
           putative 
          Length = 465

 Score = 29.9 bits (64), Expect = 1.0
 Identities = 22/82 (26%), Positives = 36/82 (43%), Gaps = 1/82 (1%)
 Frame = +2

Query: 5   KGFEPQQRI-KLARMTALWIGNGCVPPSVLLVLVNEHLLKDNLALEFVLEVFATVKQERG 181
           K  E Q R+ KL +   L +  G +    +++ V + ++K NL L     +   V   R 
Sbjct: 378 KETERQMRVSKLVKGEILMLNIGSMSTGAMVIGVKKDMVKVNLTLPVCTSIGEKVAISRR 437

Query: 182 VASLIAALKRGQLEGRLLEFLP 247
           V      + RGQ+E     F+P
Sbjct: 438 VDRHWRLIGRGQIEAGTTIFIP 459


>At1g34720.1 68414.m04316 hypothetical protein
          Length = 176

 Score = 28.3 bits (60), Expect = 3.2
 Identities = 14/44 (31%), Positives = 27/44 (61%), Gaps = 1/44 (2%)
 Frame = +2

Query: 224 GRLLEFLPLNRRSEDVLATSFASRGLAELLRLHRAQA-SQEARR 352
           G  L+FLP++ +  ++ +T  A   +AE++  H +Q  ++ ARR
Sbjct: 129 GNHLDFLPISEKFWEMTSTLEAGHAVAEMVMYHISQLHTENARR 172


>At4g05300.1 68417.m00803 hypothetical protein
          Length = 387

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
 Frame = +2

Query: 224 GRLLEFLPLNRRSEDVLATSFASRGLAELLRLHRAQA-SQEARR 352
           G  L+FLP++ +  ++  T  A   +AE++  H +Q  ++ ARR
Sbjct: 129 GNHLDFLPISEKFWEMTPTLEAGHAVAEMVMYHISQLHTENARR 172


>At3g43380.1 68416.m04591 hypothetical protein
          Length = 215

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
 Frame = +2

Query: 224 GRLLEFLPLNRRSEDVLATSFASRGLAELLRLHRAQA-SQEARR 352
           G  L+FLP++ +  ++  T  A   +AE++  H +Q  ++ ARR
Sbjct: 129 GNHLDFLPISEKFWEMTPTLEAGHAVAEMVMYHISQLHTENARR 172


>At3g24370.1 68416.m03060 hypothetical protein
          Length = 187

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
 Frame = +2

Query: 224 GRLLEFLPLNRRSEDVLATSFASRGLAELLRLHRAQA-SQEARR 352
           G  L+FLP++ +  ++  T  A   +AE++  H +Q  ++ ARR
Sbjct: 129 GNHLDFLPISEKFWEMTPTLEAGHAVAEMVMYHISQLHTENARR 172


>At1g27800.1 68414.m03403 hypothetical protein
          Length = 211

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 14/44 (31%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
 Frame = +2

Query: 224 GRLLEFLPLNRRSEDVLATSFASRGLAELLRLHRAQA-SQEARR 352
           G  L+FLP++ +  ++  T  A   +AE++  H +Q  ++ ARR
Sbjct: 129 GNHLDFLPISEKFWEMTPTLEAGHAVAEMVMYHISQLHTENARR 172


>At5g52550.1 68418.m06525 expressed protein
          Length = 360

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 23/78 (29%), Positives = 39/78 (50%)
 Frame = +2

Query: 251 NRRSEDVLATSFASRGLAELLRLHRAQASQEARRELTQALLDELAEEKPVRDLIQDLRDM 430
           NRR E  +ATS A R   E  +  + +   EA  E   A   + A++K  RD ++ ++  
Sbjct: 37  NRRLEKAIATSAAIRAELEKKKQMKKEGQLEAADEEDSA---DAAKKKQERDELERIKQA 93

Query: 431 ALKHSIPEHETIAINMAV 484
             K +  E ++IA + A+
Sbjct: 94  ENKKNRLE-KSIATSAAI 110


>At3g43680.1 68416.m04657 hypothetical protein
          Length = 539

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 15/34 (44%), Positives = 21/34 (61%)
 Frame = +2

Query: 326 AQASQEARRELTQALLDELAEEKPVRDLIQDLRD 427
           A+ASQ ARRE ++A L   AE +    LI+ L +
Sbjct: 423 ARASQMARREASEAKLKAAAETRGNAQLIKALEE 456


>At3g58610.1 68416.m06532 ketol-acid reductoisomerase identical to
           ketol-acid reductoisomerase, chloroplast precursor (EC
           1.1.1.86) (Acetohydroxy-acid reductoisomerase)
           (Alpha-keto-beta-hydroxylacil reductoisomerase)
           (Swiss-Prot:Q05758) [Arabidopsis thaliana]
          Length = 591

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 16/54 (29%), Positives = 24/54 (44%)
 Frame = +2

Query: 8   GFEPQQRIKLARMTALWIGNGCVPPSVLLVLVNEHLLKDNLALEFVLEVFATVK 169
           GF       L  +TA   GNG    S+   +V+   +K  ++L+F   VF   K
Sbjct: 38  GFLSSSSKSLRSLTATVAGNGATGSSLAARMVSSSAVKAPVSLDFETSVFKKEK 91


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,985,914
Number of Sequences: 28952
Number of extensions: 117465
Number of successful extensions: 445
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 440
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 445
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 917929344
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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