BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I09A02NGRL0001_O01 (508 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 35 0.006 SPCC31H12.06 |mug111||sequence orphan|Schizosaccharomyces pombe|... 27 2.1 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 3.7 SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomy... 26 3.7 SPAC2F3.13c |||queuine tRNA-ribosyltransferase |Schizosaccharomy... 25 8.6 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 35.1 bits (77), Expect = 0.006 Identities = 16/30 (53%), Positives = 16/30 (53%) Frame = +3 Query: 90 GNFGLSGHGPSGFGHRPSGFGHGPSGFVHG 179 G G G GP GF P GFG GP GF G Sbjct: 221 GGHGGFGGGPGGFEGGPGGFGGGPGGFGGG 250 Score = 34.7 bits (76), Expect = 0.008 Identities = 19/35 (54%), Positives = 19/35 (54%) Frame = +3 Query: 78 GLSPGNFGLSGHGPSGFGHRPSGFGHGPSGFVHGG 182 G PG FG G GFG P GFG GP G HGG Sbjct: 241 GGGPGGFG---GGLGGFGGGPGGFGGGPGG--HGG 270 Score = 33.9 bits (74), Expect = 0.014 Identities = 17/31 (54%), Positives = 17/31 (54%) Frame = +3 Query: 87 PGNFGLSGHGPSGFGHRPSGFGHGPSGFVHG 179 PG FG GP GFG GFG GP GF G Sbjct: 237 PGGFG---GGPGGFGGGLGGFGGGPGGFGGG 264 Score = 33.1 bits (72), Expect = 0.025 Identities = 17/33 (51%), Positives = 17/33 (51%), Gaps = 3/33 (9%) Frame = +3 Query: 90 GNFGLSGH---GPSGFGHRPSGFGHGPSGFVHG 179 G FG GH G GFG P GF GP GF G Sbjct: 211 GGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGG 243 Score = 33.1 bits (72), Expect = 0.025 Identities = 17/34 (50%), Positives = 17/34 (50%) Frame = +3 Query: 78 GLSPGNFGLSGHGPSGFGHRPSGFGHGPSGFVHG 179 G G G G GP GFG P GFG G GF G Sbjct: 225 GFGGGPGGFEG-GPGGFGGGPGGFGGGLGGFGGG 257 Score = 25.4 bits (53), Expect = 4.9 Identities = 16/40 (40%), Positives = 18/40 (45%), Gaps = 4/40 (10%) Frame = +3 Query: 78 GLSPGNFGLSGHGPSGFGHRPSGF----GHGPSGFVHGGN 185 G + G FG G G G P GF G G G HGG+ Sbjct: 184 GHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGH 223 >SPCC31H12.06 |mug111||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 468 Score = 26.6 bits (56), Expect = 2.1 Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 2/45 (4%) Frame = -3 Query: 302 GYILS*ITFFKLANCKTLLFLRYM--FEIMLTIAFLFSGLYITTM 174 G+I S TF L C LFL Y+ F + + + GL TT+ Sbjct: 322 GFINSVKTFGSLLTCSVCLFLTYVGGFSVHMMKTTMLIGLTATTL 366 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 25.8 bits (54), Expect = 3.7 Identities = 14/56 (25%), Positives = 33/56 (58%) Frame = +1 Query: 181 VIYKPLKRKAMVSMISNM*RKNNKVLQLASLKKVI*DRIYPSNKIPDKVNMNNKLE 348 ++ L+ K + + +N + ++ + ++ S++ I +R+ SNK P+ +MN +LE Sbjct: 1010 LVVNALEDKKLAATPANDVQNDSLIYRIKSVQDEI-NRLSTSNKSPEVASMNVRLE 1064 >SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomyces pombe|chr 2|||Manual Length = 1019 Score = 25.8 bits (54), Expect = 3.7 Identities = 11/51 (21%), Positives = 28/51 (54%), Gaps = 1/51 (1%) Frame = +3 Query: 195 TEEESDGQHDFEHVAQEQQSLAVGELEEGDLGQD-IPIQQDSRQSEHEQQA 344 ++EE + ++ A E+ + E+E+ + G+D +++ +RQ + + A Sbjct: 958 SDEEEEESEEYSEDASEEDGYSESEVEDEESGEDWDELERKARQEDAKHDA 1008 >SPAC2F3.13c |||queuine tRNA-ribosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 348 Score = 24.6 bits (51), Expect = 8.6 Identities = 10/20 (50%), Positives = 16/20 (80%) Frame = -2 Query: 288 LNHLLQARQLQDFVVLALHV 229 + HLLQAR+L +++L L+V Sbjct: 320 VRHLLQARELVAWILLQLYV 339 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,626,153 Number of Sequences: 5004 Number of extensions: 31636 Number of successful extensions: 97 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 85 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 93 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 202220600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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