BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I09A02NGRL0001_N19 (500 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha... 187 7e-49 SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar... 186 1e-48 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 31 0.13 SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo... 31 0.13 SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pomb... 29 0.52 SPBC12C2.09c |||Haemolysin-III family protein|Schizosaccharomyce... 27 1.2 SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |S... 27 1.6 SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.1 SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac... 27 2.1 SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogam... 26 2.8 SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 25 4.8 SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 25 4.8 SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3... 25 4.8 SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 25 6.4 SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom... 25 8.4 SPAC13G7.06 |met16||phosphoadenosine phosphosulfate reductase|Sc... 25 8.4 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 25 8.4 >SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 187 bits (456), Expect = 7e-49 Identities = 94/164 (57%), Positives = 117/164 (71%) Frame = +2 Query: 2 ISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTKLGPQRIHLVRSRGGNTKYRALRLDT 181 I+RD HKR ATG KRA RKKRK+ELGR +NT++GP+RIH VR RGGN K+RALRLD+ Sbjct: 3 ITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDS 62 Query: 182 GNFAWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYLLPLG 361 GNF+WGSE ++KTRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G Sbjct: 63 GNFSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG 122 Query: 362 RKKGAKLTEAEEAIINEKRSRKTAKKYLSRQRLSKVEGGLEEQF 493 KG K T +S+ +K+ +R SKV+ LE QF Sbjct: 123 -SKGKKATSTP-----NPKSKHVQRKHSARLGDSKVDSALETQF 160 >SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 186 bits (454), Expect = 1e-48 Identities = 94/164 (57%), Positives = 117/164 (71%) Frame = +2 Query: 2 ISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTKLGPQRIHLVRSRGGNTKYRALRLDT 181 I+RD HKR ATG KRA RKKRK+ELGR +NT++GP+RIH VR RGGN K+RALRLD+ Sbjct: 3 ITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDS 62 Query: 182 GNFAWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYLLPLG 361 GNF+WGSE ++KTRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G Sbjct: 63 GNFSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG 122 Query: 362 RKKGAKLTEAEEAIINEKRSRKTAKKYLSRQRLSKVEGGLEEQF 493 KG K T +S+ +K+ +R SKV+ LE QF Sbjct: 123 -SKGKKATATP-----TPKSKHVQRKHSARLGDSKVDSALETQF 160 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 30.7 bits (66), Expect = 0.13 Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 2/76 (2%) Frame = +2 Query: 8 RDHWHKRRATGGKRAPIRKKRKY--ELGRPAANTKLGPQRIHLVRSRGGNTKYRALRLDT 181 RD +K + G K+ + EL +N + Q+ L+ S T YRA+ LDT Sbjct: 1083 RDRLYKSQLIGRVLDDTTKENRLLKELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDT 1142 Query: 182 GNFAWGSECSTRKTRI 229 G+ E + K RI Sbjct: 1143 GDLMAVKEVALHKPRI 1158 >SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual Length = 616 Score = 30.7 bits (66), Expect = 0.13 Identities = 15/43 (34%), Positives = 23/43 (53%) Frame = +2 Query: 353 PLGRKKGAKLTEAEEAIINEKRSRKTAKKYLSRQRLSKVEGGL 481 PL R+K +LT E + E S T+KK+ + + SK G+ Sbjct: 569 PLSRRKARQLTALELGVSPEALSSATSKKHNKKNKRSKQRSGV 611 >SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pombe|chr 1|||Manual Length = 508 Score = 28.7 bits (61), Expect = 0.52 Identities = 18/61 (29%), Positives = 32/61 (52%), Gaps = 1/61 (1%) Frame = +2 Query: 299 VVVDATPFRQWYESHYLLPLGRKKGAKLTEAE-EAIINEKRSRKTAKKYLSRQRLSKVEG 475 V DA ++ E ++ +KK +KL EA+ E+ EK+ +K K S+++ + E Sbjct: 434 VTKDAEESQEDVEMDIVIEKKKKKSSKLKEADGESSKKEKKEKKDKKHKKSKRKSEESED 493 Query: 476 G 478 G Sbjct: 494 G 494 >SPBC12C2.09c |||Haemolysin-III family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 324 Score = 27.5 bits (58), Expect = 1.2 Identities = 9/19 (47%), Positives = 11/19 (57%) Frame = -1 Query: 98 WQPGDLTHTSSSYEWAHVC 42 W PG SS++W HVC Sbjct: 278 WSPGKYDVFGSSHQWFHVC 296 >SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |Schizosaccharomyces pombe|chr 1|||Manual Length = 672 Score = 27.1 bits (57), Expect = 1.6 Identities = 25/83 (30%), Positives = 39/83 (46%) Frame = +2 Query: 89 PAANTKLGPQRIHLVRSRGGNTKYRALRLDTGNFAWGSECSTRKTRIIDVVYNASNNELV 268 P NT + PQ RSR T R+L +G + S ++ +TR V +AS+N L+ Sbjct: 530 PLQNTVISPQPTR--RSR---TPIRSL---SGRSSVASSRNSSRTRSYSNVSSASSNSLI 581 Query: 269 RTKTLVKNAIVVVDATPFRQWYE 337 + ++ V TPF +E Sbjct: 582 SIISSKPSSSTVQRQTPFHSPFE 604 >SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 689 Score = 26.6 bits (56), Expect = 2.1 Identities = 11/23 (47%), Positives = 16/23 (69%) Frame = -2 Query: 301 DNSILYQGFGTHQFIVRGIINNI 233 +NS+ +Q F +H FI R +IN I Sbjct: 541 ENSLYHQSFSSHWFISRLLINPI 563 >SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 26.6 bits (56), Expect = 2.1 Identities = 23/86 (26%), Positives = 32/86 (37%), Gaps = 3/86 (3%) Frame = +2 Query: 11 DHWHKRRATGGKRAPIRKKRKY---ELGRPAANTKLGPQRIHLVRSRGGNTKYRALRLDT 181 D W +A G +PIR+ ++Y +L P K H R K R + + Sbjct: 758 DEWKASKAFGANDSPIRENKRYKHNKLQTPKPADKF-RDNYHKQNKRNREAKERGIGIKV 816 Query: 182 GNFAWGSECSTRKTRIIDVVYNASNN 259 N S RK R + A NN Sbjct: 817 -NSELKSAVEIRKARELKEKRLAKNN 841 >SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogamy |Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 26.2 bits (55), Expect = 2.8 Identities = 9/19 (47%), Positives = 12/19 (63%) Frame = -2 Query: 337 FIPLPERCGIHHDNSILYQ 281 F+P PE C I+H+ I Q Sbjct: 477 FLPAPEECNIYHNEHIQVQ 495 >SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces pombe|chr 1|||Manual Length = 1679 Score = 25.4 bits (53), Expect = 4.8 Identities = 10/14 (71%), Positives = 11/14 (78%) Frame = -2 Query: 394 LSLSELGTFLPSEW 353 LSL+E GTFL EW Sbjct: 1554 LSLNECGTFLQKEW 1567 >SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 817 Score = 25.4 bits (53), Expect = 4.8 Identities = 15/61 (24%), Positives = 31/61 (50%), Gaps = 2/61 (3%) Frame = +2 Query: 194 WGSEC--STRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYLLPLGRK 367 WGS+ +K + +V+ +++ + +T L AT F + +S+Y +PL R+ Sbjct: 250 WGSQSYLQEKKAGLAEVLNISTSQQKPKTTALDYEVFRHEMATKFSSFADSYYPVPLDRE 309 Query: 368 K 370 + Sbjct: 310 Q 310 >SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 555 Score = 25.4 bits (53), Expect = 4.8 Identities = 26/103 (25%), Positives = 42/103 (40%) Frame = +2 Query: 107 LGPQRIHLVRSRGGNTKYRALRLDTGNFAWGSECSTRKTRIIDVVYNASNNELVRTKTLV 286 LGP + V+ G + + + L N A R + + + S L+ T+ V Sbjct: 249 LGPIGKYFVKKYGFSPNCQIIPLTGDNPATILSLPLRPGKDVLLSLGTSTTALMATQNYV 308 Query: 287 KNAIVVVDATPFRQWYESHYLLPLGRKKGAKLTEAEEAIINEK 415 + + A P Q +HY++ L K G+ E INEK Sbjct: 309 CSPEYHMFAHPVTQ---NHYMVMLCYKNGSLAREQVRNTINEK 348 >SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 1016 Score = 25.0 bits (52), Expect = 6.4 Identities = 10/15 (66%), Positives = 11/15 (73%) Frame = +2 Query: 344 YLLPLGRKKGAKLTE 388 Y+LPLGRK K TE Sbjct: 492 YILPLGRKTARKCTE 506 >SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1021 Score = 24.6 bits (51), Expect = 8.4 Identities = 13/59 (22%), Positives = 28/59 (47%) Frame = +2 Query: 311 ATPFRQWYESHYLLPLGRKKGAKLTEAEEAIINEKRSRKTAKKYLSRQRLSKVEGGLEE 487 +TP + H + KKG+K+ A++ E+R + A++ + + K +E+ Sbjct: 754 STPDTTGSDIHIVSEKRGKKGSKVITAKKVSAKERREARRARRQTALEESLKAPISIED 812 >SPAC13G7.06 |met16||phosphoadenosine phosphosulfate reductase|Schizosaccharomyces pombe|chr 1|||Manual Length = 266 Score = 24.6 bits (51), Expect = 8.4 Identities = 10/17 (58%), Positives = 12/17 (70%), Gaps = 1/17 (5%) Frame = -3 Query: 150 LPPRDLTKWMRWG-PSL 103 L P+D+ KW RW PSL Sbjct: 32 LSPQDILKWCRWTLPSL 48 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 24.6 bits (51), Expect = 8.4 Identities = 17/65 (26%), Positives = 31/65 (47%) Frame = +2 Query: 11 DHWHKRRATGGKRAPIRKKRKYELGRPAANTKLGPQRIHLVRSRGGNTKYRALRLDTGNF 190 + W K T K+ +KRK L R A +L + + + S+GG +A + ++ Sbjct: 32 EKWSKGVKTN-KKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAKKNSSLD 90 Query: 191 AWGSE 205 A+ +E Sbjct: 91 AFLNE 95 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,248,592 Number of Sequences: 5004 Number of extensions: 48036 Number of successful extensions: 139 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 139 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 198176188 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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