SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0001_N19
         (500 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha...   187   7e-49
SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar...   186   1e-48
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki...    31   0.13 
SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo...    31   0.13 
SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pomb...    29   0.52 
SPBC12C2.09c |||Haemolysin-III family protein|Schizosaccharomyce...    27   1.2  
SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |S...    27   1.6  
SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    27   2.1  
SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac...    27   2.1  
SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogam...    26   2.8  
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc...    25   4.8  
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce...    25   4.8  
SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3...    25   4.8  
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom...    25   6.4  
SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom...    25   8.4  
SPAC13G7.06 |met16||phosphoadenosine phosphosulfate reductase|Sc...    25   8.4  
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|...    25   8.4  

>SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  187 bits (456), Expect = 7e-49
 Identities = 94/164 (57%), Positives = 117/164 (71%)
 Frame = +2

Query: 2   ISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTKLGPQRIHLVRSRGGNTKYRALRLDT 181
           I+RD  HKR ATG KRA  RKKRK+ELGR  +NT++GP+RIH VR RGGN K+RALRLD+
Sbjct: 3   ITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDS 62

Query: 182 GNFAWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYLLPLG 361
           GNF+WGSE  ++KTRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G
Sbjct: 63  GNFSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG 122

Query: 362 RKKGAKLTEAEEAIINEKRSRKTAKKYLSRQRLSKVEGGLEEQF 493
             KG K T          +S+   +K+ +R   SKV+  LE QF
Sbjct: 123 -SKGKKATSTP-----NPKSKHVQRKHSARLGDSKVDSALETQF 160


>SPAC521.05 |rps802|rps8-2|40S ribosomal protein
           S8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 200

 Score =  186 bits (454), Expect = 1e-48
 Identities = 94/164 (57%), Positives = 117/164 (71%)
 Frame = +2

Query: 2   ISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTKLGPQRIHLVRSRGGNTKYRALRLDT 181
           I+RD  HKR ATG KRA  RKKRK+ELGR  +NT++GP+RIH VR RGGN K+RALRLD+
Sbjct: 3   ITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDS 62

Query: 182 GNFAWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYLLPLG 361
           GNF+WGSE  ++KTRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G
Sbjct: 63  GNFSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG 122

Query: 362 RKKGAKLTEAEEAIINEKRSRKTAKKYLSRQRLSKVEGGLEEQF 493
             KG K T          +S+   +K+ +R   SKV+  LE QF
Sbjct: 123 -SKGKKATATP-----TPKSKHVQRKHSARLGDSKVDSALETQF 160


>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase
            Win1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1436

 Score = 30.7 bits (66), Expect = 0.13
 Identities = 23/76 (30%), Positives = 34/76 (44%), Gaps = 2/76 (2%)
 Frame = +2

Query: 8    RDHWHKRRATGGKRAPIRKKRKY--ELGRPAANTKLGPQRIHLVRSRGGNTKYRALRLDT 181
            RD  +K +  G       K+ +   EL    +N  +  Q+  L+ S    T YRA+ LDT
Sbjct: 1083 RDRLYKSQLIGRVLDDTTKENRLLKELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDT 1142

Query: 182  GNFAWGSECSTRKTRI 229
            G+     E +  K RI
Sbjct: 1143 GDLMAVKEVALHKPRI 1158


>SPAC3F10.16c |||GTP binding protein,
           HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 616

 Score = 30.7 bits (66), Expect = 0.13
 Identities = 15/43 (34%), Positives = 23/43 (53%)
 Frame = +2

Query: 353 PLGRKKGAKLTEAEEAIINEKRSRKTAKKYLSRQRLSKVEGGL 481
           PL R+K  +LT  E  +  E  S  T+KK+  + + SK   G+
Sbjct: 569 PLSRRKARQLTALELGVSPEALSSATSKKHNKKNKRSKQRSGV 611


>SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 508

 Score = 28.7 bits (61), Expect = 0.52
 Identities = 18/61 (29%), Positives = 32/61 (52%), Gaps = 1/61 (1%)
 Frame = +2

Query: 299 VVVDATPFRQWYESHYLLPLGRKKGAKLTEAE-EAIINEKRSRKTAKKYLSRQRLSKVEG 475
           V  DA   ++  E   ++   +KK +KL EA+ E+   EK+ +K  K   S+++  + E 
Sbjct: 434 VTKDAEESQEDVEMDIVIEKKKKKSSKLKEADGESSKKEKKEKKDKKHKKSKRKSEESED 493

Query: 476 G 478
           G
Sbjct: 494 G 494


>SPBC12C2.09c |||Haemolysin-III family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 324

 Score = 27.5 bits (58), Expect = 1.2
 Identities = 9/19 (47%), Positives = 11/19 (57%)
 Frame = -1

Query: 98  WQPGDLTHTSSSYEWAHVC 42
           W PG      SS++W HVC
Sbjct: 278 WSPGKYDVFGSSHQWFHVC 296


>SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 672

 Score = 27.1 bits (57), Expect = 1.6
 Identities = 25/83 (30%), Positives = 39/83 (46%)
 Frame = +2

Query: 89  PAANTKLGPQRIHLVRSRGGNTKYRALRLDTGNFAWGSECSTRKTRIIDVVYNASNNELV 268
           P  NT + PQ     RSR   T  R+L   +G  +  S  ++ +TR    V +AS+N L+
Sbjct: 530 PLQNTVISPQPTR--RSR---TPIRSL---SGRSSVASSRNSSRTRSYSNVSSASSNSLI 581

Query: 269 RTKTLVKNAIVVVDATPFRQWYE 337
              +   ++  V   TPF   +E
Sbjct: 582 SIISSKPSSSTVQRQTPFHSPFE 604


>SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 689

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 11/23 (47%), Positives = 16/23 (69%)
 Frame = -2

Query: 301 DNSILYQGFGTHQFIVRGIINNI 233
           +NS+ +Q F +H FI R +IN I
Sbjct: 541 ENSLYHQSFSSHWFISRLLINPI 563


>SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 848

 Score = 26.6 bits (56), Expect = 2.1
 Identities = 23/86 (26%), Positives = 32/86 (37%), Gaps = 3/86 (3%)
 Frame = +2

Query: 11   DHWHKRRATGGKRAPIRKKRKY---ELGRPAANTKLGPQRIHLVRSRGGNTKYRALRLDT 181
            D W   +A G   +PIR+ ++Y   +L  P    K      H    R    K R + +  
Sbjct: 758  DEWKASKAFGANDSPIRENKRYKHNKLQTPKPADKF-RDNYHKQNKRNREAKERGIGIKV 816

Query: 182  GNFAWGSECSTRKTRIIDVVYNASNN 259
             N    S    RK R +     A NN
Sbjct: 817  -NSELKSAVEIRKARELKEKRLAKNN 841


>SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogamy
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 543

 Score = 26.2 bits (55), Expect = 2.8
 Identities = 9/19 (47%), Positives = 12/19 (63%)
 Frame = -2

Query: 337 FIPLPERCGIHHDNSILYQ 281
           F+P PE C I+H+  I  Q
Sbjct: 477 FLPAPEECNIYHNEHIQVQ 495


>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1679

 Score = 25.4 bits (53), Expect = 4.8
 Identities = 10/14 (71%), Positives = 11/14 (78%)
 Frame = -2

Query: 394  LSLSELGTFLPSEW 353
            LSL+E GTFL  EW
Sbjct: 1554 LSLNECGTFLQKEW 1567


>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 817

 Score = 25.4 bits (53), Expect = 4.8
 Identities = 15/61 (24%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
 Frame = +2

Query: 194 WGSEC--STRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYLLPLGRK 367
           WGS+     +K  + +V+  +++ +  +T  L         AT F  + +S+Y +PL R+
Sbjct: 250 WGSQSYLQEKKAGLAEVLNISTSQQKPKTTALDYEVFRHEMATKFSSFADSYYPVPLDRE 309

Query: 368 K 370
           +
Sbjct: 310 Q 310


>SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 555

 Score = 25.4 bits (53), Expect = 4.8
 Identities = 26/103 (25%), Positives = 42/103 (40%)
 Frame = +2

Query: 107 LGPQRIHLVRSRGGNTKYRALRLDTGNFAWGSECSTRKTRIIDVVYNASNNELVRTKTLV 286
           LGP   + V+  G +   + + L   N A       R  + + +    S   L+ T+  V
Sbjct: 249 LGPIGKYFVKKYGFSPNCQIIPLTGDNPATILSLPLRPGKDVLLSLGTSTTALMATQNYV 308

Query: 287 KNAIVVVDATPFRQWYESHYLLPLGRKKGAKLTEAEEAIINEK 415
            +    + A P  Q   +HY++ L  K G+   E     INEK
Sbjct: 309 CSPEYHMFAHPVTQ---NHYMVMLCYKNGSLAREQVRNTINEK 348


>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1016

 Score = 25.0 bits (52), Expect = 6.4
 Identities = 10/15 (66%), Positives = 11/15 (73%)
 Frame = +2

Query: 344 YLLPLGRKKGAKLTE 388
           Y+LPLGRK   K TE
Sbjct: 492 YILPLGRKTARKCTE 506


>SPCC132.01c ||SPCC1322.17c|DUF814 family
           protein|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1021

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 13/59 (22%), Positives = 28/59 (47%)
 Frame = +2

Query: 311 ATPFRQWYESHYLLPLGRKKGAKLTEAEEAIINEKRSRKTAKKYLSRQRLSKVEGGLEE 487
           +TP     + H +     KKG+K+  A++    E+R  + A++  + +   K    +E+
Sbjct: 754 STPDTTGSDIHIVSEKRGKKGSKVITAKKVSAKERREARRARRQTALEESLKAPISIED 812


>SPAC13G7.06 |met16||phosphoadenosine phosphosulfate
           reductase|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 266

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 10/17 (58%), Positives = 12/17 (70%), Gaps = 1/17 (5%)
 Frame = -3

Query: 150 LPPRDLTKWMRWG-PSL 103
           L P+D+ KW RW  PSL
Sbjct: 32  LSPQDILKWCRWTLPSL 48


>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 207

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 17/65 (26%), Positives = 31/65 (47%)
 Frame = +2

Query: 11  DHWHKRRATGGKRAPIRKKRKYELGRPAANTKLGPQRIHLVRSRGGNTKYRALRLDTGNF 190
           + W K   T  K+    +KRK  L R A   +L  + +  + S+GG    +A + ++   
Sbjct: 32  EKWSKGVKTN-KKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAKKNSSLD 90

Query: 191 AWGSE 205
           A+ +E
Sbjct: 91  AFLNE 95


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,248,592
Number of Sequences: 5004
Number of extensions: 48036
Number of successful extensions: 139
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 198176188
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -