Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0001_N19
         (500 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) riboso...   197   4e-51
At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S ri...   190   3e-49
At1g73260.1 68414.m08478 trypsin and protease inhibitor family p...    29   1.3  
At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / sta...    29   2.3  
At1g22830.1 68414.m02850 pentatricopeptide (PPR) repeat-containi...    27   5.4  
At5g27240.1 68418.m03249 DNAJ heat shock N-terminal domain-conta...    27   7.1  
At4g32820.1 68417.m04668 expressed protein ; expression supporte...    27   7.1  
At4g25730.1 68417.m03703 FtsJ-like methyltransferase family prot...    27   7.1  
At4g11490.1 68417.m01847 protein kinase family protein contains ...    27   7.1  
At3g21750.1 68416.m02744 UDP-glucoronosyl/UDP-glucosyl transfera...    27   7.1  
At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta...    27   7.1  

>At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) ribosomal
           protein S8 - Zea mays, PIR:T04088
          Length = 222

 Score =  197 bits (480), Expect = 4e-51
 Identities = 104/175 (59%), Positives = 125/175 (71%), Gaps = 11/175 (6%)
 Frame = +2

Query: 2   ISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTKLGPQR-IHLVRSRGGNTKYRALRLD 178
           ISRD  HKRRATGGK+   RKKRKYE+GR  ANTKL   + +  +R RGGN K+RALRLD
Sbjct: 3   ISRDSIHKRRATGGKQKQWRKKRKYEMGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLD 62

Query: 179 TGNFAWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYLLPL 358
           TGN++WGSE +TRKTR++DVVYNASNNELVRTKTLVK+AIV VDA PF+QWY SHY + L
Sbjct: 63  TGNYSWGSEATTRKTRVLDVVYNASNNELVRTKTLVKSAIVQVDAAPFKQWYLSHYGVEL 122

Query: 359 GR-KKGAKLT-----EAEEAII----NEKRSRKTAKKYLSRQRLSKVEGGLEEQF 493
           GR KK A  T     E EEA +      K+S    +K  SRQ    ++  +E+QF
Sbjct: 123 GRKKKSASSTKKDGEEGEEAAVAAPEEVKKSNHLLRKIASRQEGRSLDSHIEDQF 177


>At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S
           ribosomal protein S8, Prunus armeniaca, EMBL:AF071889
          Length = 210

 Score =  190 bits (464), Expect = 3e-49
 Identities = 99/165 (60%), Positives = 116/165 (70%), Gaps = 1/165 (0%)
 Frame = +2

Query: 2   ISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTKLGPQR-IHLVRSRGGNTKYRALRLD 178
           ISRD  HKRRATGGK+   RKKRKYELGR  ANTKL   + +  +R RGGN K+RALRLD
Sbjct: 3   ISRDSIHKRRATGGKQKMWRKKRKYELGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLD 62

Query: 179 TGNFAWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYLLPL 358
           TGNF+WGSE  TRKTRI+DV YNASNNELVRT+TLVK+AIV VDA PF+Q Y  HY + +
Sbjct: 63  TGNFSWGSEAVTRKTRILDVAYNASNNELVRTQTLVKSAIVQVDAAPFKQGYLQHYGVDI 122

Query: 359 GRKKGAKLTEAEEAIINEKRSRKTAKKYLSRQRLSKVEGGLEEQF 493
           GRKK  +    EE     K+S    +K   RQ    ++  LEEQF
Sbjct: 123 GRKKKGEAVTTEEV----KKSNHVQRKLEMRQEGRALDSHLEEQF 163


>At1g73260.1 68414.m08478 trypsin and protease inhibitor family
           protein / Kunitz family protein similar to trypsin
           inhibitor propeptide [Brassica oleracea] GI:841208;
           contains Pfam profile PF00197: Trypsin and protease
           inhibitor
          Length = 215

 Score = 29.5 bits (63), Expect = 1.3
 Identities = 18/44 (40%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
 Frame = +2

Query: 263 LVRTKTLVKNAI-VVVDATPFRQWYESHYLLPLGRKKGAKLTEA 391
           L  T  L  NA   VVD      ++ES+Y+LP+ R +G  LT A
Sbjct: 16  LALTAVLASNAYGAVVDIDGNAMFHESYYVLPVIRGRGGGLTLA 59


>At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / starch
           branching enzyme class II (SBE2-2) identical to starch
           branching enzyme class II [Arabidopsis thaliana]
           GI:726490
          Length = 716

 Score = 28.7 bits (61), Expect = 2.3
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = -1

Query: 98  WQPGDLTHTSSSYEWAHVCRP*PSSYANDHD 6
           WQ GD+ +T ++  W+  C     SYA  HD
Sbjct: 459 WQMGDIIYTLTNRRWSEKC----ISYAESHD 485


>At1g22830.1 68414.m02850 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat.  Gene
           continues on the 3' end of BAC F19G10 gb|AF000657 gene
           F19G10.21
          Length = 703

 Score = 27.5 bits (58), Expect = 5.4
 Identities = 12/35 (34%), Positives = 20/35 (57%)
 Frame = -2

Query: 394 LSLSELGTFLPSEWQQVVAFIPLPERCGIHHDNSI 290
           L++SE+  F+P  W+Q+    P+ E    H D S+
Sbjct: 13  LTVSEICKFIPQSWKQLPR--PISETSKTHDDESV 45


>At5g27240.1 68418.m03249 DNAJ heat shock N-terminal
           domain-containing protein
          Length = 1104

 Score = 27.1 bits (57), Expect = 7.1
 Identities = 14/38 (36%), Positives = 17/38 (44%)
 Frame = -1

Query: 278 FWYAPIHC*RHYKQHQ*YESCGLNIQIPMRSFQYQDAG 165
           FW    HC   YK  + Y +  LN  I  RS+   D G
Sbjct: 159 FWTCCEHCGYRYKYLRKYVNILLNCNICQRSYMAYDTG 196


>At4g32820.1 68417.m04668 expressed protein ; expression supported by
            MPSS
          Length = 1817

 Score = 27.1 bits (57), Expect = 7.1
 Identities = 14/37 (37%), Positives = 18/37 (48%)
 Frame = +3

Query: 327  SGMKATTCCHSEGRKVPSSLRLRKPS*TKNAVERQRR 437
            +G    +CC+     VPS+ R RK S     VE  RR
Sbjct: 1100 AGSFIRSCCYCYFENVPSNSRWRKNSALSQRVETSRR 1136


>At4g25730.1 68417.m03703 FtsJ-like methyltransferase family protein
           contains Pfam profile: PF01728 FtsJ-like
           methyltransferase
          Length = 821

 Score = 27.1 bits (57), Expect = 7.1
 Identities = 12/47 (25%), Positives = 24/47 (51%)
 Frame = +2

Query: 326 QWYESHYLLPLGRKKGAKLTEAEEAIINEKRSRKTAKKYLSRQRLSK 466
           QW+  +       +      ++E+ I N+K+S+  +K   S+Q+ SK
Sbjct: 539 QWFSQNIFAEAVEEGDLGKDDSEDEIANKKKSKNLSKPDKSKQKASK 585


>At4g11490.1 68417.m01847 protein kinase family protein contains
           Pfam PF00069: Protein kinase domain
          Length = 636

 Score = 27.1 bits (57), Expect = 7.1
 Identities = 12/46 (26%), Positives = 22/46 (47%)
 Frame = -3

Query: 318 GVASTTTIAFFTKVLVRTNSLLEAL*TTSIIRVLRVEHSDPHAKFP 181
           G     ++ FF  ++V T  ++  L T  ++ V R   +DP  + P
Sbjct: 258 GSKRNISVGFFVAIVVATGVVISVLSTLVVVLVCRKRKTDPPEESP 303


>At3g21750.1 68416.m02744 UDP-glucoronosyl/UDP-glucosyl transferase
           family protein contains Pfam profile: PF00201
           UDP-glucoronosyl and UDP-glucosyl transferase
          Length = 473

 Score = 27.1 bits (57), Expect = 7.1
 Identities = 20/62 (32%), Positives = 32/62 (51%)
 Frame = -3

Query: 315 VASTTTIAFFTKVLVRTNSLLEAL*TTSIIRVLRVEHSDPHAKFPVSRRRARYLVLPPRD 136
           + +TT +A   K+LV +++ L     T I+   RV      + +  S  R RY++LP RD
Sbjct: 16  IRATTALA---KLLVASDNRLSV---TLIVIPSRVSDDASSSVYTNSEDRLRYILLPARD 69

Query: 135 LT 130
            T
Sbjct: 70  QT 71


>At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch
           branching enzyme class II (SBE2-1) nearly identical to
           starch branching enzyme class II [Arabidopsis thaliana]
           GI:619939
          Length = 858

 Score = 27.1 bits (57), Expect = 7.1
 Identities = 12/31 (38%), Positives = 15/31 (48%)
 Frame = -1

Query: 98  WQPGDLTHTSSSYEWAHVCRP*PSSYANDHD 6
           WQ GD+T T ++  W   C      YA  HD
Sbjct: 583 WQVGDITFTLTNRRWGEKC----VVYAESHD 609


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,277,700
Number of Sequences: 28952
Number of extensions: 271749
Number of successful extensions: 693
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 675
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 690
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 888318720
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)