BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I09A02NGRL0001_N01 (558 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyce... 229 2e-61 SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomy... 124 1e-29 SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2 ... 84 2e-17 SPAPB17E12.10c |||SAM-dependent methyltransferase|Schizosaccharo... 29 0.35 SPBC6B1.08c |ofd1||2-oxoglutarate and Fe|Schizosaccharomyces pom... 27 1.4 SPBC543.05c |||inorganic anion exchanger |Schizosaccharomyces po... 27 1.9 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 27 1.9 SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 27 1.9 SPBC25B2.03 |||zf-C3HC4 type zinc finger|Schizosaccharomyces pom... 26 3.3 SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual 26 4.3 SPAC31G5.18c |||ubiquitin family, human C1ORF55 related|Schizosa... 25 5.7 SPAC22A12.01c |pso2|snm1, SPAC56F8.17c, snm1|DNA 5' exonuclease ... 25 7.5 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 25 7.5 SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr 1||... 25 10.0 >SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 229 bits (561), Expect = 2e-61 Identities = 114/179 (63%), Positives = 138/179 (77%), Gaps = 4/179 (2%) Frame = +2 Query: 26 MGKTSKDKRDIYYRLAKEEGWRARSAFKLLQINEEYNIFDNVLRAVDLCAAPGSWSQVLT 205 MG++SKDKRD YYRLAKE+GWRARSAFKLLQ+NE++N+F+ R VDLCAAPGSWSQVL+ Sbjct: 1 MGRSSKDKRDAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCAAPGSWSQVLS 60 Query: 206 KTLRQNAENV----EDVKIVAVDLQAMAALPGVKQIQGDITKLSTACSIIKEFEGLKADL 373 + L +N + E IVAVDLQ MA + GV +Q DIT +T I+ F ADL Sbjct: 61 RELLKNIDTSIAADEKPMIVAVDLQPMAPIDGVCTLQLDITHPNTLSIILSHFGNEPADL 120 Query: 374 VVCDGAPDVTGLHDIDEYVQSQLLLAALHITTHVLKIGGVFVAKIFRGKDVSLLYSQLK 550 VV DGAPDVTGLHD+DEY+Q+Q+LLAA ++ VLK GG FVAKIFRG+DVSLLYSQL+ Sbjct: 121 VVSDGAPDVTGLHDLDEYIQAQILLAAFNLAVCVLKPGGKFVAKIFRGRDVSLLYSQLR 179 >SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 802 Score = 124 bits (298), Expect = 1e-29 Identities = 67/174 (38%), Positives = 100/174 (57%) Frame = +2 Query: 32 KTSKDKRDIYYRLAKEEGWRARSAFKLLQINEEYNIFDNVLRAVDLCAAPGSWSQVLTKT 211 KT+K + D +Y+LAKE+G+R+R+AFKL+Q+N++Y+ + +DLCAAPG W QV +KT Sbjct: 7 KTAKGRLDKWYKLAKEQGYRSRAAFKLVQLNQKYSFLEKAKVIIDLCAAPGGWLQVASKT 66 Query: 212 LRQNAENVEDVKIVAVDLQAMAALPGVKQIQGDITKLSTACSIIKEFEGLKADLVVCDGA 391 + + IV VDL + +P DIT + + KAD+V+ DGA Sbjct: 67 CKPGS------LIVGVDLAPIKPIPNCHTFVEDITSDKCRSQLRGYLKTWKADVVLHDGA 120 Query: 392 PDVTGLHDIDEYVQSQLLLAALHITTHVLKIGGVFVAKIFRGKDVSLLYSQLKQ 553 P+V D Y Q+QL+L ++ + L GG FV K+FR +D + L KQ Sbjct: 121 PNVGSAWLQDAYGQAQLVLMSMKLACEFLVAGGTFVTKVFRSRDYNNLLWVFKQ 174 >SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 218 Score = 83.8 bits (198), Expect = 2e-17 Identities = 55/183 (30%), Positives = 91/183 (49%), Gaps = 12/183 (6%) Frame = +2 Query: 38 SKDKRDIYYRLAKEEGWRARSAFKLLQINEEYNIFDNVLRAVDLCAAPGSWSQVLTKTLR 217 +K +D Y + +K + +R+R+A+KL+++N +Y + +D+ APGSWSQV K + Sbjct: 12 AKRSKDFYRKKSKIDNFRSRAAYKLIELNSKYRFINKEDVVIDVGFAPGSWSQVAKKLVG 71 Query: 218 QNAENVEDVKIVAVDLQAMAALPGVKQIQGDI------TKLSTACSIIKE------FEGL 361 K++ +D+Q +A GV I GDI TKL A ++ E + Sbjct: 72 NKG------KVIGIDIQHIAPPEGVLPIYGDIRDPNTLTKLFEALRLLHEPNTNDSIDCR 125 Query: 362 KADLVVCDGAPDVTGLHDIDEYVQSQLLLAALHITTHVLKIGGVFVAKIFRGKDVSLLYS 541 D V+ D TG+ D + +L +ALH+ LK G F+ K + G + + L + Sbjct: 126 VVDAVISDMLHKATGIRIRDHALSMELCASALHVALTFLKSNGSFICKFYMGDEDADLQN 185 Query: 542 QLK 550 LK Sbjct: 186 LLK 188 >SPAPB17E12.10c |||SAM-dependent methyltransferase|Schizosaccharomyces pombe|chr 1|||Manual Length = 301 Score = 29.5 bits (63), Expect = 0.35 Identities = 20/77 (25%), Positives = 33/77 (42%) Frame = +2 Query: 116 QINEEYNIFDNVLRAVDLCAAPGSWSQVLTKTLRQNAENVEDVKIVAVDLQAMAALPGVK 295 +IN+E+ +F VDL APG WS + + + +++A D+ Sbjct: 65 KINKEHQLFKPGQIVVDLGCAPGIWSTIAARHVGLFG------RVIACDIIPCRLPENSS 118 Query: 296 QIQGDITKLSTACSIIK 346 IQG+I + I K Sbjct: 119 MIQGNILSMEIQLEIAK 135 >SPBC6B1.08c |ofd1||2-oxoglutarate and Fe|Schizosaccharomyces pombe|chr 2|||Manual Length = 515 Score = 27.5 bits (58), Expect = 1.4 Identities = 10/33 (30%), Positives = 17/33 (51%) Frame = -1 Query: 381 HTTRSAFRPSNSFIIEHAVDSFVISPCICLTPG 283 H FRP + F + D+ ++ +CL+PG Sbjct: 399 HQVGRRFRPGSDFTLATTNDTALLEATLCLSPG 431 >SPBC543.05c |||inorganic anion exchanger |Schizosaccharomyces pombe|chr 2|||Manual Length = 517 Score = 27.1 bits (57), Expect = 1.9 Identities = 14/31 (45%), Positives = 19/31 (61%) Frame = +2 Query: 458 HITTHVLKIGGVFVAKIFRGKDVSLLYSQLK 550 HIT +I G++VA I+ K V +L QLK Sbjct: 149 HITKFSCEIFGLYVAFIYLEKGVQVLCDQLK 179 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 27.1 bits (57), Expect = 1.9 Identities = 19/64 (29%), Positives = 34/64 (53%) Frame = -1 Query: 423 SSMSCSPVTSGAPSHTTRSAFRPSNSFIIEHAVDSFVISPCICLTPGNAAIACKSTATIL 244 SS S S +S + S ++ S+ RPS+S + S + +TP +++ ST++ + Sbjct: 212 SSTSSSHSSSSSSSSSSSSSSRPSSSSSFITTMSSSTFISTVTVTPSSSS---SSTSSEV 268 Query: 243 TSST 232 SST Sbjct: 269 PSST 272 >SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 317 Score = 27.1 bits (57), Expect = 1.9 Identities = 23/77 (29%), Positives = 36/77 (46%) Frame = -1 Query: 456 NAANRSCDCTYSSMSCSPVTSGAPSHTTRSAFRPSNSFIIEHAVDSFVISPCICLTPGNA 277 ++++ S + SS S S +S +PS + S+ S+SFI S S I A Sbjct: 189 SSSSSSSSKSSSSSSSSSKSSASPSSSKSSSKFSSSSFITSTTPASSSSSGAIVSNAKTA 248 Query: 276 AIACKSTATILTSSTFS 226 + S+A+ TSS S Sbjct: 249 STDDSSSASSATSSVSS 265 >SPBC25B2.03 |||zf-C3HC4 type zinc finger|Schizosaccharomyces pombe|chr 2|||Manual Length = 554 Score = 26.2 bits (55), Expect = 3.3 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = +2 Query: 389 APDVTGLHDIDEYVQSQLLLAALH 460 A D T +H++DEY++ Q L +H Sbjct: 466 AADETRVHNVDEYIEQQDLDELIH 489 >SPAC23C4.17 |||tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 25.8 bits (54), Expect = 4.3 Identities = 13/41 (31%), Positives = 26/41 (63%) Frame = +2 Query: 161 VDLCAAPGSWSQVLTKTLRQNAENVEDVKIVAVDLQAMAAL 283 +D+CAAPGS + L + + + A N++D + +L+++ L Sbjct: 164 LDMCAAPGSKTAQLIEAVYKKA-NIKDAAHDSKNLKSVEGL 203 >SPAC31G5.18c |||ubiquitin family, human C1ORF55 related|Schizosaccharomyces pombe|chr 1|||Manual Length = 263 Score = 25.4 bits (53), Expect = 5.7 Identities = 10/38 (26%), Positives = 21/38 (55%) Frame = +3 Query: 21 EKWVKLQKINEIFTID*LKKKVGEHEVLSNCYKSMKSI 134 +K K QK+N++ D + +HE L + +S+ ++ Sbjct: 143 KKEAKKQKLNKVLAADSSSSRFDDHEYLEDLEQSVSNV 180 >SPAC22A12.01c |pso2|snm1, SPAC56F8.17c, snm1|DNA 5' exonuclease |Schizosaccharomyces pombe|chr 1|||Manual Length = 560 Score = 25.0 bits (52), Expect = 7.5 Identities = 13/39 (33%), Positives = 22/39 (56%) Frame = +2 Query: 107 KLLQINEEYNIFDNVLRAVDLCAAPGSWSQVLTKTLRQN 223 K L++N+ YNI + +D PGS + + +TL+ N Sbjct: 255 KRLKLNQPYNIMGITVYVLDANHCPGS-AMFVFETLQSN 292 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 25.0 bits (52), Expect = 7.5 Identities = 18/73 (24%), Positives = 33/73 (45%) Frame = -1 Query: 453 AANRSCDCTYSSMSCSPVTSGAPSHTTRSAFRPSNSFIIEHAVDSFVISPCICLTPGNAA 274 A+N + + +S S + TS + ++ S+ SNS S + S ++ Sbjct: 186 ASNSATSSSLASSSLNSTTSATATSSSLSSTAASNSATSSSLASSSLNSTTSATATSSSI 245 Query: 273 IACKSTATILTSS 235 + S++T LTSS Sbjct: 246 SSTVSSSTPLTSS 258 >SPAC17D4.04 ||SPAC458.01|tRNA |Schizosaccharomyces pombe|chr 1|||Manual Length = 654 Score = 24.6 bits (51), Expect = 10.0 Identities = 11/30 (36%), Positives = 19/30 (63%) Frame = +2 Query: 155 RAVDLCAAPGSWSQVLTKTLRQNAENVEDV 244 + +D+CAAPGS + L + L + + ED+ Sbjct: 146 KVLDMCAAPGSKTAQLLEALHKPTKK-EDI 174 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,119,568 Number of Sequences: 5004 Number of extensions: 40206 Number of successful extensions: 136 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 128 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 132 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 233995432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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