BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I09A02NGRL0001_M18 (603 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 83 1e-16 At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 83 1e-16 At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 83 1e-16 At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 83 1e-16 At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 83 1e-16 At5g37410.1 68418.m04500 hypothetical protein contains Pfam PF04... 31 0.44 At1g48910.1 68414.m05479 flavin-containing monooxygenase family ... 29 2.4 At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplas... 29 3.1 At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplas... 29 3.1 At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplas... 29 3.1 At5g40450.1 68418.m04905 expressed protein 27 9.6 At4g32010.1 68417.m04557 transcriptional factor B3 family protei... 27 9.6 At3g16540.1 68416.m02112 DegP protease, putative contains simila... 27 9.6 At1g03960.2 68414.m00382 calcium-binding EF hand family protein ... 27 9.6 At1g03960.1 68414.m00381 calcium-binding EF hand family protein ... 27 9.6 >At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2) identical to S-adenosylmethionine synthetase 2 (Methionine adenosyltransferase 2, AdoMet synthetase 2) [Arabidopsis thaliana] SWISS-PROT:P17562 Length = 393 Score = 83.0 bits (196), Expect = 1e-16 Identities = 36/45 (80%), Positives = 42/45 (93%) Frame = +2 Query: 449 FLFTSESVGEGHPDQMCDQISDAILDAHLKQDPNAKVACETVTKT 583 FLFTSESV EGHPD++CDQISDA+LDA L+QDP++KVACET TKT Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKT 48 >At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative similar to S-adenosylmethionine synthetase 2 (Methionine adenosyltransferase 2, AdoMet synthetase 2) [Catharanthus roseus] SWISS-PROT:Q96552 Length = 393 Score = 83.0 bits (196), Expect = 1e-16 Identities = 37/45 (82%), Positives = 41/45 (91%) Frame = +2 Query: 449 FLFTSESVGEGHPDQMCDQISDAILDAHLKQDPNAKVACETVTKT 583 FLFTSESV EGHPD++CDQISDAILDA L+QDP +KVACET TKT Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKT 48 >At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative similar to S-adenosylmethionine synthetase 3 (Methionine adenosyltransferase 3, AdoMet synthetase 3) [Lycopersicon esculentum] SWISS-PROT:P43282 Length = 390 Score = 83.0 bits (196), Expect = 1e-16 Identities = 37/45 (82%), Positives = 41/45 (91%) Frame = +2 Query: 449 FLFTSESVGEGHPDQMCDQISDAILDAHLKQDPNAKVACETVTKT 583 FLFTSESV EGHPD++CDQISDAILDA L+QDP +KVACET TKT Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKT 48 >At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1) identical to S-adenosylmethionine synthetase 1 (Methionine adenosyltransferase 1, AdoMet synthetase 1) [Arabidopsis thaliana] SWISS-PROT:P23686 Length = 393 Score = 83.0 bits (196), Expect = 1e-16 Identities = 36/45 (80%), Positives = 42/45 (93%) Frame = +2 Query: 449 FLFTSESVGEGHPDQMCDQISDAILDAHLKQDPNAKVACETVTKT 583 FLFTSESV EGHPD++CDQISDA+LDA L+QDP++KVACET TKT Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKT 48 >At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1) identical to S-adenosylmethionine synthetase 1 (Methionine adenosyltransferase 1, AdoMet synthetase 1) [Arabidopsis thaliana] SWISS-PROT:P23686 Length = 393 Score = 83.0 bits (196), Expect = 1e-16 Identities = 36/45 (80%), Positives = 42/45 (93%) Frame = +2 Query: 449 FLFTSESVGEGHPDQMCDQISDAILDAHLKQDPNAKVACETVTKT 583 FLFTSESV EGHPD++CDQISDA+LDA L+QDP++KVACET TKT Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKT 48 >At5g37410.1 68418.m04500 hypothetical protein contains Pfam PF04510: Family of unknown function (DUF577); common family comprised of At5g37410, At5g37400, At5g37920, At5g37460, At5g37650, At5g37470, At5g37420, At5g37430 Length = 615 Score = 31.5 bits (68), Expect = 0.44 Identities = 28/94 (29%), Positives = 49/94 (52%), Gaps = 1/94 (1%) Frame = +2 Query: 35 VAYQKYLNNIS*KLINHKCLFSGLVTKVKASQLLVIIIK*QIR-LT*SKQNIVTNSRREL 211 +AY K +N++ K + + + GLV + A + + II+K Q+ + S+ +V L Sbjct: 512 IAY-KMINSV--KELVERGMEVGLVRR--AFRDVEIIVKDQLEWYSTSEYKLVKGLLWRL 566 Query: 212 GAQQDIYWLKIFVLWRLTDLINIGVFSRQASFPQ 313 A + + W FVLWR+ +I+ GV + PQ Sbjct: 567 YAIKGMRWESKFVLWRINVIIDRGVKEEEKELPQ 600 >At1g48910.1 68414.m05479 flavin-containing monooxygenase family protein / FMO family protein similar to flavin monoxygenase-like protein floozy [Petunia x hybrida] GI:15010541 Length = 383 Score = 29.1 bits (62), Expect = 2.4 Identities = 22/84 (26%), Positives = 34/84 (40%), Gaps = 1/84 (1%) Frame = +2 Query: 266 DLINIGVFS-RQASFPQC*FRGKSFGLFL*ANSKFRMPETSKIMNGYAKTNGHSYDMEDG 442 DL G+F +Q F F GK+ + + K R E I G NG + E+G Sbjct: 241 DLSKYGLFRPKQGPFATKLFTGKAPVIDVGTVEKIRDGEIQVINGGIGSINGKTLTFENG 300 Query: 443 SVFLFTSESVGEGHPDQMCDQISD 514 F + G+ +C+ + D Sbjct: 301 HKQDFDAIVFATGYKSSVCNWLED 324 >At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplast, putative strong similiarity to chloroplast NADP-dependent malate dehydrogenase (EC 1.1.1.82) SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum}, SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489 {Flaveria bidentis}, [Flaveria trinervia] GI:726334, SP|P17606I {Sorghum bicolor}; contains InterPro entry IPR001236: Lactate/malate dehydrogenase Length = 334 Score = 28.7 bits (61), Expect = 3.1 Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 2/65 (3%) Frame = +2 Query: 410 TNGHSYDMEDGSVFLFTSESVGEGHPDQMCD-QISDAILDAHLKQDPNAKVACETVTK-T 583 T+G+ Y +E+G VF S G+G + + D +I D + K + V T Sbjct: 255 TDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRIAKSEAELLAEKRCVAHLT 314 Query: 584 GNGIA 598 G GIA Sbjct: 315 GEGIA 319 >At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplast, putative strong similiarity to chloroplast NADP-dependent malate dehydrogenase (EC 1.1.1.82) SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum}, SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489 {Flaveria bidentis}, [Flaveria trinervia] GI:726334, SP|P17606I {Sorghum bicolor}; contains InterPro entry IPR001236: Lactate/malate dehydrogenase Length = 442 Score = 28.7 bits (61), Expect = 3.1 Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 2/65 (3%) Frame = +2 Query: 410 TNGHSYDMEDGSVFLFTSESVGEGHPDQMCD-QISDAILDAHLKQDPNAKVACETVTK-T 583 T+G+ Y +E+G VF S G+G + + D +I D + K + V T Sbjct: 363 TDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRIAKSEAELLAEKRCVAHLT 422 Query: 584 GNGIA 598 G GIA Sbjct: 423 GEGIA 427 >At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplast, putative strong similiarity to chloroplast NADP-dependent malate dehydrogenase (EC 1.1.1.82) SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum}, SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489 {Flaveria bidentis}, [Flaveria trinervia] GI:726334, SP|P17606I {Sorghum bicolor}; contains InterPro entry IPR001236: Lactate/malate dehydrogenase Length = 443 Score = 28.7 bits (61), Expect = 3.1 Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 2/65 (3%) Frame = +2 Query: 410 TNGHSYDMEDGSVFLFTSESVGEGHPDQMCD-QISDAILDAHLKQDPNAKVACETVTK-T 583 T+G+ Y +E+G VF S G+G + + D +I D + K + V T Sbjct: 364 TDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRIAKSEAELLAEKRCVAHLT 423 Query: 584 GNGIA 598 G GIA Sbjct: 424 GEGIA 428 >At5g40450.1 68418.m04905 expressed protein Length = 2910 Score = 27.1 bits (57), Expect = 9.6 Identities = 13/28 (46%), Positives = 18/28 (64%) Frame = +2 Query: 434 EDGSVFLFTSESVGEGHPDQMCDQISDA 517 E+ S+ + T ESVGE P + D+I DA Sbjct: 1955 EERSISVETEESVGETKPKEHEDEIRDA 1982 >At4g32010.1 68417.m04557 transcriptional factor B3 family protein low similarity to FUSCA3 [Arabidopsis thaliana] GI:3582518, VIVIPAROUS1 protein [Triticum aestivum] GI:7801376; contains Pfam profile PF02362: B3 DNA binding domain Length = 780 Score = 27.1 bits (57), Expect = 9.6 Identities = 8/18 (44%), Positives = 16/18 (88%) Frame = -1 Query: 378 SGILNLELAHKNNPKDFP 325 SG++++ ++H++N KDFP Sbjct: 97 SGLISMNVSHESNGKDFP 114 >At3g16540.1 68416.m02112 DegP protease, putative contains similarity to DegP2 protease GI:13172275 from [Arabidopsis thaliana] Length = 555 Score = 27.1 bits (57), Expect = 9.6 Identities = 14/49 (28%), Positives = 22/49 (44%) Frame = +1 Query: 262 HRPYKYRRILAPGLFSTVLIQGEIFRVIFVS*L*IQNAGDIKNYEWICK 408 H +Y R PGL S++ V+ S + AG + Y +IC+ Sbjct: 8 HTVGRYSRARVPGLLSSLFFYRSCNNVLTNSLPTVTTAGRVSRYGYICR 56 >At1g03960.2 68414.m00382 calcium-binding EF hand family protein contains Pfam profile: PF00036 EF hand Length = 389 Score = 27.1 bits (57), Expect = 9.6 Identities = 16/43 (37%), Positives = 21/43 (48%), Gaps = 6/43 (13%) Frame = -3 Query: 337 ERFPPELTLWKRGLAREYA------DIYKVGEAPQDEYFEPID 227 ER P LT W R REYA D+ +V D + EP++ Sbjct: 344 EREDPNLTEWDRFAQREYARLSMEEDVDEVSNGSADVWDEPLE 386 >At1g03960.1 68414.m00381 calcium-binding EF hand family protein contains Pfam profile: PF00036 EF hand Length = 529 Score = 27.1 bits (57), Expect = 9.6 Identities = 16/43 (37%), Positives = 21/43 (48%), Gaps = 6/43 (13%) Frame = -3 Query: 337 ERFPPELTLWKRGLAREYA------DIYKVGEAPQDEYFEPID 227 ER P LT W R REYA D+ +V D + EP++ Sbjct: 484 EREDPNLTEWDRFAQREYARLSMEEDVDEVSNGSADVWDEPLE 526 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,619,304 Number of Sequences: 28952 Number of extensions: 256488 Number of successful extensions: 553 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 544 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 553 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1197101088 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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