Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_L08
(678 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC644.13c |||Rab GTPase binding |Schizosaccharomyces pombe|chr... 110 2e-25
SPCC61.04c |||Rab GTPase binding|Schizosaccharomyces pombe|chr 3... 40 4e-04
SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb... 34 0.022
SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 28 1.4
SPAC25B8.09 |||trans-aconitate 3-methyltransferase |Schizosaccha... 28 1.4
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 27 1.9
SPAC22E12.09c |krp1|krp|kexin|Schizosaccharomyces pombe|chr 1|||... 27 2.5
SPBC800.10c |||EPS15 repeat family actin cortical patch componen... 27 2.5
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 27 3.3
SPAC343.12 |rds1||conserved fungal protein|Schizosaccharomyces p... 26 4.4
SPAC1002.11 |gaa1||GPI-anchor transamidase complex subunit Gaa1 ... 25 7.6
>SPAC644.13c |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 225
Score = 110 bits (264), Expect = 2e-25
Identities = 58/142 (40%), Positives = 83/142 (58%)
Frame = +2
Query: 131 IEGEMTIPNRAAPSGDSMEFNTLDEPIKETFHRDLRAVGNKFYHVLIPREKSSLLKEWDL 310
I GE + R GDS LDEPI+ T + RA+G K +VL P+ + +L++WDL
Sbjct: 41 IAGESIVETRFT-GGDS-----LDEPIRVTLFNEFRAIGEKLVYVLYPKN-AQVLRDWDL 93
Query: 311 WGPLMLCTLMATMLQGSTDTADSVNDGGPEFAEVFVIVWIGAAVVTLNSKLLGGNISFFQ 490
WGPL+ ++A L STD + + F V ++W G AV +LN KLLG NIS FQ
Sbjct: 94 WGPLIFSLVIALALALSTDKIERES----VFTVVVALIWFGEAVCSLNIKLLGANISIFQ 149
Query: 491 SVCVLGYCLLPVAVSLIICRVI 556
S+C+LGY P+ ++ I+C +
Sbjct: 150 SMCILGYSSFPLMIASIVCAFV 171
>SPCC61.04c |||Rab GTPase binding|Schizosaccharomyces pombe|chr
3|||Manual
Length = 227
Score = 39.5 bits (88), Expect = 4e-04
Identities = 30/111 (27%), Positives = 54/111 (48%), Gaps = 3/111 (2%)
Frame = +2
Query: 242 VGNKFYHVLIPREKSS--LLKEWDLWGPLMLCTLMATMLQGSTDTADSVNDGGPEFAEVF 415
+ K HVL P + ++ + D+ GP++ C L +T L S++ G F ++
Sbjct: 71 IKQKTTHVLNPFKHVDVHIMDDTDMAGPILFCLLFSTFL--------SLH-GRSHFGYIY 121
Query: 416 VIVWIGAAVVTLNSKLLGG-NISFFQSVCVLGYCLLPVAVSLIICRVILFS 565
I +G+ + +L+ N+ F ++V VLGY LLP+ V + F+
Sbjct: 122 GIALLGSLSLHFVLRLMSAKNLFFTRTVSVLGYSLLPLVVIAFFKNIFTFN 172
>SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1254
Score = 33.9 bits (74), Expect = 0.022
Identities = 28/103 (27%), Positives = 45/103 (43%), Gaps = 6/103 (5%)
Frame = -1
Query: 564 LNRITRQMIRETATGRRQYPNTQTDWKNEML-PPSSLEFKVTTAAPIHTIT-----NTSA 403
+N+ + RE T + NT+ D + ML PPSS F ++ +H + ++
Sbjct: 66 VNKNSSSPERELPTSPSHHANTEIDSSSSMLPPPSSDPFSSPLSSSLHRSSPKRPHDSLG 125
Query: 402 NSGPPSLTESAVSVEPWSIVAINVQSISGPHRSHSFNKLDFSL 274
P L ++V EP S + I P + SF LD S+
Sbjct: 126 EESPGKLLRTSVKQEPDS-----EEEIDSPTKKKSFKSLDTSI 163
>SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence
orphan|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1563
Score = 27.9 bits (59), Expect = 1.4
Identities = 14/53 (26%), Positives = 24/53 (45%)
Frame = -1
Query: 501 TQTDWKNEMLPPSSLEFKVTTAAPIHTITNTSANSGPPSLTESAVSVEPWSIV 343
TQ E ++ E + TTA + T T + +G + +A V+P + V
Sbjct: 1346 TQVTTGTETQATTATETQATTATEVQTTTGATGTAGTETQVTTATEVQPTTAV 1398
Score = 27.1 bits (57), Expect = 2.5
Identities = 13/49 (26%), Positives = 22/49 (44%)
Frame = -1
Query: 501 TQTDWKNEMLPPSSLEFKVTTAAPIHTITNTSANSGPPSLTESAVSVEP 355
TQ E ++ E + TTA + T T + +G + +A V+P
Sbjct: 1182 TQVTTGTETQATTATETQATTATEVQTTTGATGTAGTETQATTATEVQP 1230
Score = 27.1 bits (57), Expect = 2.5
Identities = 13/49 (26%), Positives = 22/49 (44%)
Frame = -1
Query: 501 TQTDWKNEMLPPSSLEFKVTTAAPIHTITNTSANSGPPSLTESAVSVEP 355
TQ E ++ E + TTA + T T + +G + +A V+P
Sbjct: 1264 TQVTTGTETQATTATETQATTATEVQTTTGATGTAGTETQATTATEVQP 1312
>SPAC25B8.09 |||trans-aconitate 3-methyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 251
Score = 27.9 bits (59), Expect = 1.4
Identities = 22/78 (28%), Positives = 35/78 (44%)
Frame = -1
Query: 567 VLNRITRQMIRETATGRRQYPNTQTDWKNEMLPPSSLEFKVTTAAPIHTITNTSANSGPP 388
++ R + I + T R YP T+W N+ EF V + TI A SG
Sbjct: 1 MIGRTFKTDIADYETARPDYPPQITEWLND-------EFSVNETS---TILELGAGSG-- 48
Query: 387 SLTESAVSVEPWSIVAIN 334
LT ++ +P I+A++
Sbjct: 49 KLTPRIIASQPKEIIAVD 66
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 27.5 bits (58), Expect = 1.9
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +2
Query: 578 FPFLLEARHITGRLHVGY 631
FP LL +ITG+LH+G+
Sbjct: 60 FPILLPPPNITGKLHIGH 77
>SPAC22E12.09c |krp1|krp|kexin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 709
Score = 27.1 bits (57), Expect = 2.5
Identities = 18/66 (27%), Positives = 29/66 (43%)
Frame = -1
Query: 552 TRQMIRETATGRRQYPNTQTDWKNEMLPPSSLEFKVTTAAPIHTITNTSANSGPPSLTES 373
T ++ +E G PN+ + +L P+S F T + T T+TS P L +
Sbjct: 599 TLELPKEMVLGIYSEPNSDLTNSSTLLSPTSTSFTSYTVSATATPTSTSHIPIPTVLPPT 658
Query: 372 AVSVEP 355
+EP
Sbjct: 659 QPVLEP 664
>SPBC800.10c |||EPS15 repeat family actin cortical patch component
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1116
Score = 27.1 bits (57), Expect = 2.5
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = -1
Query: 507 PNTQTDWKNEMLPPSSLEFKVTTAAPIHTITNTSANSGPPSLTESA 370
P+ T ++L + F T AP HT +N + +S PP +T+SA
Sbjct: 383 PSRVTSGTEDLLSLDATPFS-PTLAPQHTSSNATKHSAPP-VTKSA 426
>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 26.6 bits (56), Expect = 3.3
Identities = 15/52 (28%), Positives = 24/52 (46%), Gaps = 1/52 (1%)
Frame = -1
Query: 507 PNTQTDWKNEMLPPSSLEFKVTTAAPIHTITNTSANSGPPSL-TESAVSVEP 355
P+T + +PP++ TT P T NT+ + PP+ S +V P
Sbjct: 73 PSTSHNSTTTTVPPTT-SMNTTTTVPPTTSLNTTTTTAPPTTHVNSTTTVVP 123
Score = 25.8 bits (54), Expect = 5.8
Identities = 15/67 (22%), Positives = 26/67 (38%)
Frame = -1
Query: 555 ITRQMIRETATGRRQYPNTQTDWKNEMLPPSSLEFKVTTAAPIHTITNTSANSGPPSLTE 376
+T +T + T T+ + + PPS+ TT P T NT+ P +
Sbjct: 44 VTTTSFSSASTETSTHSVTSTNITSIVPPPSTSHNSTTTTVPPTTSMNTTTTVPPTTSLN 103
Query: 375 SAVSVEP 355
+ + P
Sbjct: 104 TTTTTAP 110
>SPAC343.12 |rds1||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 402
Score = 26.2 bits (55), Expect = 4.4
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -1
Query: 384 LTESAVSVEPWSIVAINVQSISGP 313
L + A+ V P++I AIN ++GP
Sbjct: 373 LVDEAIPVTPFNITAINEHVVAGP 396
>SPAC1002.11 |gaa1||GPI-anchor transamidase complex subunit Gaa1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 581
Score = 25.4 bits (53), Expect = 7.6
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +3
Query: 573 QHFLFFLRLVISLVGFMW 626
Q LFFL+L ++L+G W
Sbjct: 16 QRHLFFLQLSLTLIGLSW 33
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,874,379
Number of Sequences: 5004
Number of extensions: 60853
Number of successful extensions: 178
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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