BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I09A02NGRL0001_K05 (528 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic ac... 24 1.1 AY395072-1|AAQ96728.1| 593|Apis mellifera GABA neurotransmitter... 23 2.6 AY395071-1|AAQ96727.1| 646|Apis mellifera GABA neurotransmitter... 23 2.6 >AY540846-1|AAS48080.1| 541|Apis mellifera neuronal nicotinic acetylcholine receptorApisa2 subunit protein. Length = 541 Score = 23.8 bits (49), Expect = 1.1 Identities = 9/24 (37%), Positives = 13/24 (54%) Frame = +3 Query: 105 DMLHHEFVQAMNGFNKTTKHNKGL 176 D H+ + NG + TT HN+ L Sbjct: 395 DSARHQRIGGCNGLHTTTAHNRFL 418 >AY395072-1|AAQ96728.1| 593|Apis mellifera GABA neurotransmitter transporter-1B protein. Length = 593 Score = 22.6 bits (46), Expect = 2.6 Identities = 7/15 (46%), Positives = 10/15 (66%) Frame = -1 Query: 171 PCCVWWFC*SRSSPA 127 PCC W C + ++PA Sbjct: 487 PCCWWKICWTITTPA 501 Score = 21.0 bits (42), Expect = 7.8 Identities = 7/16 (43%), Positives = 12/16 (75%) Frame = -1 Query: 114 ATCLRTCSASLTYIIL 67 ATC+ +C ++ YII+ Sbjct: 103 ATCVLSCWTNIYYIII 118 >AY395071-1|AAQ96727.1| 646|Apis mellifera GABA neurotransmitter transporter-1B protein. Length = 646 Score = 22.6 bits (46), Expect = 2.6 Identities = 7/15 (46%), Positives = 10/15 (66%) Frame = -1 Query: 171 PCCVWWFC*SRSSPA 127 PCC W C + ++PA Sbjct: 540 PCCWWKICWTITTPA 554 Score = 21.0 bits (42), Expect = 7.8 Identities = 7/16 (43%), Positives = 12/16 (75%) Frame = -1 Query: 114 ATCLRTCSASLTYIIL 67 ATC+ +C ++ YII+ Sbjct: 156 ATCVLSCWTNIYYIII 171 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 91,537 Number of Sequences: 438 Number of extensions: 1431 Number of successful extensions: 5 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5 length of database: 146,343 effective HSP length: 54 effective length of database: 122,691 effective search space used: 14845611 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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