Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_J13
(569 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51310.1 68416.m05616 vacuolar protein sorting-associated pro... 29 2.2
At1g01300.1 68414.m00046 aspartyl protease family protein contai... 29 2.9
At3g18680.2 68416.m02373 aspartate/glutamate/uridylate kinase fa... 28 5.0
At3g18680.1 68416.m02372 aspartate/glutamate/uridylate kinase fa... 28 5.0
At5g61540.2 68418.m07723 L-asparaginase, putative / L-asparagine... 27 6.7
At5g61540.1 68418.m07722 L-asparaginase, putative / L-asparagine... 27 6.7
At4g22300.1 68417.m03225 phospholipase/carboxylesterase family p... 27 6.7
At4g21440.1 68417.m03099 myb family transcription factor (MYB102... 27 8.8
>At3g51310.1 68416.m05616 vacuolar protein sorting-associated
protein 35 family protein / VPS35 family protein similar
to vacuolar protein sorting 35 [Mus musculus]
GI:11875394; contains Pfam profile PF03635: Vacuolar
protein sorting-associated protein 35
Length = 783
Score = 29.1 bits (62), Expect = 2.2
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 2/57 (3%)
Frame = +2
Query: 5 GQLNQLRLFLVNIKEPGIH*YHNYS--LMSQPETKFQCTEM*GDAHVVRANRCDNLK 169
G L ++R+F V ++ H YS L+ +P+ QC + AH+ A+ C+NLK
Sbjct: 616 GTLQRMRVFNVENRDTLTHKATGYSARLLRKPD---QCRAVYECAHLFWADECENLK 669
>At1g01300.1 68414.m00046 aspartyl protease family protein contains
Pfam domain, PF00026: eukaryotic aspartyl protease
Length = 485
Score = 28.7 bits (61), Expect = 2.9
Identities = 19/56 (33%), Positives = 27/56 (48%)
Frame = +1
Query: 199 YKTIEMSSKHHSRLDSPQANTQRCSPSYSSYQFFWSASTAGTCSLKSLVTTLNNMR 366
Y TI SS H RLDS NT+R + Y + + T G S ++L N ++
Sbjct: 192 YATIPCSSPHCRRLDSAGCNTRRKTCLY-QVSYGDGSFTVGDFSTETLTFRRNRVK 246
>At3g18680.2 68416.m02373 aspartate/glutamate/uridylate kinase
family protein similar to UMP-kinase GB:CAB38122
gi:4468612 from [Lactococcus lactis] ; contains Pfam
profile PF00696: Amino acid kinase family
Length = 366
Score = 27.9 bits (59), Expect = 5.0
Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = -3
Query: 294 LIRGITWRTSLCVCLRTVES*MMLRTHLNR-FIYY*DN*IGIPLRLSQRFALTTCASPYI 118
+ RG TW + + + ML T +N F+ IGIP R+ F ++ A PYI
Sbjct: 147 IFRGSTWAGCSGLDRSSADYIGMLATVMNAIFLQATMESIGIPTRVQTAFRMSEVAEPYI 206
>At3g18680.1 68416.m02372 aspartate/glutamate/uridylate kinase
family protein similar to UMP-kinase GB:CAB38122
gi:4468612 from [Lactococcus lactis] ; contains Pfam
profile PF00696: Amino acid kinase family
Length = 339
Score = 27.9 bits (59), Expect = 5.0
Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = -3
Query: 294 LIRGITWRTSLCVCLRTVES*MMLRTHLNR-FIYY*DN*IGIPLRLSQRFALTTCASPYI 118
+ RG TW + + + ML T +N F+ IGIP R+ F ++ A PYI
Sbjct: 147 IFRGSTWAGCSGLDRSSADYIGMLATVMNAIFLQATMESIGIPTRVQTAFRMSEVAEPYI 206
>At5g61540.2 68418.m07723 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:O02467
N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase)
(Aspartylglucosaminidase)(N4-(N-acetyl-beta-
glucosaminyl)-L-asparagine amidase) (AGA)[Spodoptera
frugiperda]
Length = 257
Score = 27.5 bits (58), Expect = 6.7
Identities = 10/35 (28%), Positives = 18/35 (51%)
Frame = +3
Query: 456 KVPITGNENHRLSALGSVDLTNQMKLGAATSGLAY 560
K P+ G NH ++ +D + +G +T+G Y
Sbjct: 111 KPPLVGPHNHDTISMAVIDRMGHIAVGTSTNGATY 145
>At5g61540.1 68418.m07722 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:O02467
N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase)
(Aspartylglucosaminidase)(N4-(N-acetyl-beta-
glucosaminyl)-L-asparagine amidase) (AGA)[Spodoptera
frugiperda]
Length = 359
Score = 27.5 bits (58), Expect = 6.7
Identities = 10/35 (28%), Positives = 18/35 (51%)
Frame = +3
Query: 456 KVPITGNENHRLSALGSVDLTNQMKLGAATSGLAY 560
K P+ G NH ++ +D + +G +T+G Y
Sbjct: 213 KPPLVGPHNHDTISMAVIDRMGHIAVGTSTNGATY 247
>At4g22300.1 68417.m03225 phospholipase/carboxylesterase family
protein similar to acyl-protein thioesterase-1 [Homo
sapiens] GI:9965372; contains Pfam profile PF02230:
Phospholipase/Carboxylesterase family
Length = 471
Score = 27.5 bits (58), Expect = 6.7
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = -3
Query: 408 LAGAPWSWPTAPADPHIVQCSNQALQRA 325
L+ A W +P+AP +P V C+N A+ R+
Sbjct: 31 LSNASWLFPSAPFNP--VTCNNGAVMRS 56
>At4g21440.1 68417.m03099 myb family transcription factor (MYB102)
contains Pfam profile: PF00249 myb-like DNA-binding
domain
Length = 350
Score = 27.1 bits (57), Expect = 8.8
Identities = 16/39 (41%), Positives = 20/39 (51%)
Frame = +1
Query: 274 PSYSSYQFFWSASTAGTCSLKSLVTTLNNMRISRSSGPT 390
PSYS F ++ S T S TTLN+ I+ SS T
Sbjct: 285 PSYSDQSFNFANSVLNTPSSSPSPTTLNSSYINSSSCST 323
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,503,731
Number of Sequences: 28952
Number of extensions: 218753
Number of successful extensions: 564
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 555
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 564
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1102220672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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