Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_J11
(387 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 213 3e-56
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 58 3e-09
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 58 3e-09
At1g72270.1 68414.m08355 expressed protein 29 1.1
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 27 3.3
At5g65720.1 68418.m08271 cysteine desulfurase, mitochondrial (NI... 27 4.4
At5g55720.1 68418.m06946 pectate lyase family protein similar to... 27 5.8
At2g44760.1 68415.m05571 expressed protein 27 5.8
At1g10190.1 68414.m01149 expressed protein similar to hypothetic... 27 5.8
At4g15050.1 68417.m02311 expressed protein contains Pfam profil... 26 7.6
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 213 bits (521), Expect = 3e-56
Identities = 97/128 (75%), Positives = 104/128 (81%)
Frame = +2
Query: 2 GNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGL 181
GN+IGKHRKNHIPRVGDFNES YYMEG+TGHPVF T +GKIAVNIC+GRHH LNW+ FGL
Sbjct: 204 GNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETVFGKIAVNICYGRHHPLNWLAFGL 263
Query: 182 NGAEIVFNPSATIAAEAGSEYMWNIEARNAAITNCYFTAAINRVGYEEFPNEFTSADGKP 361
NGAEIVFNPSAT+ SE MW IEARNAAI N YF +INRVG E FPN FTS DGKP
Sbjct: 264 NGAEIVFNPSATVGEL--SEPMWPIEARNAAIANSYFVGSINRVGTEVFPNPFTSGDGKP 321
Query: 362 AHKDLGLF 385
H D G F
Sbjct: 322 QHNDFGHF 329
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 57.6 bits (133), Expect = 3e-09
Identities = 39/117 (33%), Positives = 56/117 (47%), Gaps = 5/117 (4%)
Frame = +2
Query: 2 GNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGL 181
G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC+ + L
Sbjct: 141 GTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVL 200
Query: 182 NGAEIVFNPSATIAAEAGSEYM-----WNIEARNAAITNCYFTAAINRVGYEEFPNE 337
GAEI+F P+A I +E + + W + A N A NR+G E E
Sbjct: 201 QGAEILFYPTA-IGSEPQDQGLDSRDHWRRVMQGHAGANVVPLVASNRIGKEIIETE 256
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 57.6 bits (133), Expect = 3e-09
Identities = 39/117 (33%), Positives = 56/117 (47%), Gaps = 5/117 (4%)
Frame = +2
Query: 2 GNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGL 181
G +G +RK+HIP + E Y+ G+TG VF T++ KI V IC+ + L
Sbjct: 114 GTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVFQTKFAKIGVAICWDQWFPEAARAMVL 173
Query: 182 NGAEIVFNPSATIAAEAGSEYM-----WNIEARNAAITNCYFTAAINRVGYEEFPNE 337
GAEI+F P+A I +E + + W + A N A NR+G E E
Sbjct: 174 QGAEILFYPTA-IGSEPQDQGLDSRDHWRRVMQGHAGANVVPLVASNRIGKEIIETE 229
>At1g72270.1 68414.m08355 expressed protein
Length = 2777
Score = 29.1 bits (62), Expect = 1.1
Identities = 16/54 (29%), Positives = 27/54 (50%)
Frame = -3
Query: 379 PQVFVRGLPVSRSELIREFLITDPVDSRREVAISYSSISCFDIPHILTSRFSCY 218
P +F+RGL ++ S +RE + +D + + S DIP I + FS +
Sbjct: 1241 PDLFMRGLELTVSRNLREGTVDSEIDFADHSSTTEKIKSKMDIPDIESLAFSVF 1294
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 27.5 bits (58), Expect = 3.3
Identities = 29/111 (26%), Positives = 41/111 (36%), Gaps = 1/111 (0%)
Frame = +2
Query: 2 GNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGL 181
G +GKHRK +P + + PV+ T GKI IC+ L
Sbjct: 144 GQFLGKHRKV-MPT--SLERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLYRTALYA 200
Query: 182 NGAEIVFNPSATIAAEAGSEYMWNIEARNAAIT-NCYFTAAINRVGYEEFP 331
G EI P+A + E W + A+ C+ +A EFP
Sbjct: 201 KGIEIYCAPTADYSLE------WQASMIHIAVEGGCFVLSAHQFCKRREFP 245
>At5g65720.1 68418.m08271 cysteine desulfurase, mitochondrial (NIFS)
identical to Cysteine desulfurase, mitochondrial
precursor (SP:O49543) {Arabidopsis thaliana};
identical to cDNA GI:12656131; contains Pfam profile
PF00266: aminotransferase, class V
Length = 453
Score = 27.1 bits (57), Expect = 4.4
Identities = 17/57 (29%), Positives = 27/57 (47%)
Frame = -3
Query: 385 EQPQVFVRGLPVSRSELIREFLITDPVDSRREVAISYSSISCFDIPHILTSRFSCYG 215
E + ++G+ +S L + T P+D R A++ S I + PH SR YG
Sbjct: 39 EDESIMMKGVRISGRPLYLDMQATTPIDPRVFDAMNASQIHEYGNPH---SRTHLYG 92
>At5g55720.1 68418.m06946 pectate lyase family protein similar to
pectate lyase 1 GP:6606532 from [Musa acuminata]
Length = 392
Score = 26.6 bits (56), Expect = 5.8
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = +3
Query: 294 RRLSTGSVMRNSRMSSLRLTGSPRTKTWGC 383
RRLST +V NS SS G+P + W C
Sbjct: 29 RRLST-TVTSNSTASSCSANGNPIDECWRC 57
>At2g44760.1 68415.m05571 expressed protein
Length = 504
Score = 26.6 bits (56), Expect = 5.8
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = +2
Query: 44 VGDFNESNYYMEGNTGHPVFATRYGKIAVNICFGRHHVLNWMMFGLNGAEI 196
VG+ +ESNYY+ P F ++ + V C R V++ + F GA+I
Sbjct: 291 VGEMSESNYYLT-----PTF--KFEQCLVKGCHKRLRVVHTIEFANGGADI 334
>At1g10190.1 68414.m01149 expressed protein similar to hypothetical
protein GB:CAB10284 contains Pfam profile PF03080:
Arabidopsis proteins of unknown function
Length = 396
Score = 26.6 bits (56), Expect = 5.8
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +2
Query: 164 WMMFGLNGAEIVFNPSATIAAEAGSEYMWNIEARNAA 274
W+ FG E+ F PS+ +G+ W EA +A+
Sbjct: 283 WLQFGNAAEEVGFWPSSRFHQSSGNLVEWGGEAYSAS 319
>At4g15050.1 68417.m02311 expressed protein contains Pfam profile
PF03080: Arabidopsis proteins of unknown function
Length = 396
Score = 26.2 bits (55), Expect = 7.6
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +2
Query: 164 WMMFGLNGAEIVFNPSATIAAEAGSEYMWNIEARNAAI 277
W+ FG EI F PS+ +G+ W E +A++
Sbjct: 283 WLQFGNAAEEIGFWPSSRFHQSSGNLVEWGGEVYSASL 320
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,932,571
Number of Sequences: 28952
Number of extensions: 176912
Number of successful extensions: 474
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 465
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 473
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 547638520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -