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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0001_I20
         (514 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g40480.1 68418.m04909 expressed protein ; expression supporte...    30   0.80 
At2g21050.1 68415.m02499 amino acid permease, putative similar t...    27   5.6  
At1g12120.1 68414.m01404 expressed protein contains Pfam domain ...    27   5.6  
At5g10490.1 68418.m01215 mechanosensitive ion channel domain-con...    27   7.4  
At5g01240.2 68418.m00032 amino acid permease, putative strong si...    27   7.4  
At5g01240.1 68418.m00031 amino acid permease, putative strong si...    27   7.4  
At1g77550.1 68414.m09030 tubulin-tyrosine ligase family protein ...    27   7.4  
At5g41730.1 68418.m05074 protein kinase family protein contains ...    27   9.8  
At5g11980.1 68418.m01401 conserved oligomeric Golgi complex comp...    27   9.8  
At3g49490.1 68416.m05409 expressed protein                             27   9.8  

>At5g40480.1 68418.m04909 expressed protein ; expression supported by
            MPSS
          Length = 1919

 Score = 30.3 bits (65), Expect = 0.80
 Identities = 17/49 (34%), Positives = 23/49 (46%)
 Frame = +1

Query: 34   QIKLGAATAGLVYDNVNRHGATLTNTHIPGIGDKLSVAGKVNLFHNNDH 180
            Q K+       +Y  V+  G TLTN H+P  G K  V  + N F   +H
Sbjct: 1597 QTKVTVLFGNTIY--VDSPGETLTNVHVPAEGYKFPVKFRENKFAVTEH 1643


>At2g21050.1 68415.m02499 amino acid permease, putative similar to
           AUX1 [Arabidopsis thaliana] GI:1531758; contains Pfam
           profile PF01490: Transmembrane amino acid transporter
           protein
          Length = 483

 Score = 27.5 bits (58), Expect = 5.6
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = +1

Query: 352 KLNLFKTPSTSLDFTAGWHKFDTPFMKSSWEPSTWFSL 465
           KL L+ T +T++ +T G H      M + W+P  + S+
Sbjct: 225 KLVLYFTGATNILYTFGGHAVTVEIMHAMWKPQKFKSI 262


>At1g12120.1 68414.m01404 expressed protein contains Pfam domain
           PF05904: Plant protein of unknown function (DUF863)
          Length = 483

 Score = 27.5 bits (58), Expect = 5.6
 Identities = 20/81 (24%), Positives = 36/81 (44%), Gaps = 4/81 (4%)
 Frame = +1

Query: 22  DLTNQIKLGAATAGLVYDNVNRH--GATLTNTHIPGIGDKLSVAGKVNLFHNNDHDLSAK 195
           D  ++ ++G A  G  YD+  RH  G + TNT        +++    N+  +N+ ++  K
Sbjct: 347 DFPDKPEMGKAKPGCSYDSYERHTLGISETNTEEDFCVSSMALDELNNITRDNNKEIGLK 406

Query: 196 AFATRNMPTISH--LPSTNTV 252
               R M       LPS  ++
Sbjct: 407 LRRGRRMKNFQKEILPSLTSL 427


>At5g10490.1 68418.m01215 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein contains Pfam profile PF00924:
           Mechanosensitive ion channel
          Length = 519

 Score = 27.1 bits (57), Expect = 7.4
 Identities = 11/39 (28%), Positives = 21/39 (53%)
 Frame = +3

Query: 174 RP*FIREGVRDQKYAHHFPFTINQHCWWWSRIYVQGQDR 290
           RP  + E ++ +   +    T+ +H  WWS   ++G+DR
Sbjct: 142 RPFVLNEWIQTKIEGYEVSGTV-EHVGWWSPTIIRGEDR 179


>At5g01240.2 68418.m00032 amino acid permease, putative strong
           similarity to AUX1 GI:1531758 from [Arabidopsis
           thaliana]; contains Pfam profile PF01490: Transmembrane
           amino acid transporter protein
          Length = 408

 Score = 27.1 bits (57), Expect = 7.4
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = +1

Query: 352 KLNLFKTPSTSLDFTAGWHKFDTPFMKSSWEPSTWFSL 465
           KL L+ T +T++ +T G H      M + W+P  + S+
Sbjct: 157 KLVLYFTGATNILYTFGGHAVTVEIMHAMWKPRKFKSI 194


>At5g01240.1 68418.m00031 amino acid permease, putative strong
           similarity to AUX1 GI:1531758 from [Arabidopsis
           thaliana]; contains Pfam profile PF01490: Transmembrane
           amino acid transporter protein
          Length = 488

 Score = 27.1 bits (57), Expect = 7.4
 Identities = 12/38 (31%), Positives = 21/38 (55%)
 Frame = +1

Query: 352 KLNLFKTPSTSLDFTAGWHKFDTPFMKSSWEPSTWFSL 465
           KL L+ T +T++ +T G H      M + W+P  + S+
Sbjct: 237 KLVLYFTGATNILYTFGGHAVTVEIMHAMWKPRKFKSI 274


>At1g77550.1 68414.m09030 tubulin-tyrosine ligase family protein
           contains tubulin-tyrosine ligase family domain,
           Pfam:PF03133
          Length = 867

 Score = 27.1 bits (57), Expect = 7.4
 Identities = 11/27 (40%), Positives = 18/27 (66%)
 Frame = +1

Query: 154 VNLFHNNDHDLSAKAFATRNMPTISHL 234
           ++L + + H+L  KAF+   MP +SHL
Sbjct: 281 LDLSNRSIHNLVNKAFSVHEMPLLSHL 307


>At5g41730.1 68418.m05074 protein kinase family protein contains
           Pfam PF00069: Protein kinase domain
          Length = 711

 Score = 26.6 bits (56), Expect = 9.8
 Identities = 20/62 (32%), Positives = 30/62 (48%)
 Frame = +2

Query: 275 SRTRSVHQRAPLTLISLTRMTXIWAAN*TYSRLHPRRLTSLPAGTSSIRPS*SPLGNPAL 454
           S TRSV+  AP+  +S  + +   A       L   +L S  AGTS+++   SP  +P  
Sbjct: 614 SDTRSVYSEAPMKKVSALKKSGEMAKLRRSPSLGSEKLRS--AGTSTVKARSSPKVSPLS 671

Query: 455 GF 460
            F
Sbjct: 672 PF 673


>At5g11980.1 68418.m01401 conserved oligomeric Golgi complex
           component-related / COG complex component-related
           similar to SP|Q96MW5 Conserved oligomeric Golgi complex
           component 8 {Homo sapiens}; contains Pfam profile
           PF04124: Dor1-like family
          Length = 569

 Score = 26.6 bits (56), Expect = 9.8
 Identities = 11/39 (28%), Positives = 20/39 (51%)
 Frame = +2

Query: 200 SRPEICPPFPIYHQPTLLVVVSNICSRTRSVHQRAPLTL 316
           S+ ++ PP  +   P L V ++ + S    +   APL+L
Sbjct: 390 SKDDVTPPSYLMEHPPLAVFINGVSSALNELRPCAPLSL 428


>At3g49490.1 68416.m05409 expressed protein
          Length = 953

 Score = 26.6 bits (56), Expect = 9.8
 Identities = 16/45 (35%), Positives = 27/45 (60%)
 Frame = -3

Query: 446 GSQEDFMKGVSNLCQPAVKSSDVDGVLNRFSLPPRX*SFLLKKSV 312
           G QED ++G  ++     +SSDV  V++RF +  R  +  +KKS+
Sbjct: 847 GDQEDKLEG--SVMANMGRSSDVSDVMDRFQILKRREAEQVKKSL 889


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,618,146
Number of Sequences: 28952
Number of extensions: 250019
Number of successful extensions: 668
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 655
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 668
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 927799552
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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