BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I09A02NGRL0001_I20 (514 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g40480.1 68418.m04909 expressed protein ; expression supporte... 30 0.80 At2g21050.1 68415.m02499 amino acid permease, putative similar t... 27 5.6 At1g12120.1 68414.m01404 expressed protein contains Pfam domain ... 27 5.6 At5g10490.1 68418.m01215 mechanosensitive ion channel domain-con... 27 7.4 At5g01240.2 68418.m00032 amino acid permease, putative strong si... 27 7.4 At5g01240.1 68418.m00031 amino acid permease, putative strong si... 27 7.4 At1g77550.1 68414.m09030 tubulin-tyrosine ligase family protein ... 27 7.4 At5g41730.1 68418.m05074 protein kinase family protein contains ... 27 9.8 At5g11980.1 68418.m01401 conserved oligomeric Golgi complex comp... 27 9.8 At3g49490.1 68416.m05409 expressed protein 27 9.8 >At5g40480.1 68418.m04909 expressed protein ; expression supported by MPSS Length = 1919 Score = 30.3 bits (65), Expect = 0.80 Identities = 17/49 (34%), Positives = 23/49 (46%) Frame = +1 Query: 34 QIKLGAATAGLVYDNVNRHGATLTNTHIPGIGDKLSVAGKVNLFHNNDH 180 Q K+ +Y V+ G TLTN H+P G K V + N F +H Sbjct: 1597 QTKVTVLFGNTIY--VDSPGETLTNVHVPAEGYKFPVKFRENKFAVTEH 1643 >At2g21050.1 68415.m02499 amino acid permease, putative similar to AUX1 [Arabidopsis thaliana] GI:1531758; contains Pfam profile PF01490: Transmembrane amino acid transporter protein Length = 483 Score = 27.5 bits (58), Expect = 5.6 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +1 Query: 352 KLNLFKTPSTSLDFTAGWHKFDTPFMKSSWEPSTWFSL 465 KL L+ T +T++ +T G H M + W+P + S+ Sbjct: 225 KLVLYFTGATNILYTFGGHAVTVEIMHAMWKPQKFKSI 262 >At1g12120.1 68414.m01404 expressed protein contains Pfam domain PF05904: Plant protein of unknown function (DUF863) Length = 483 Score = 27.5 bits (58), Expect = 5.6 Identities = 20/81 (24%), Positives = 36/81 (44%), Gaps = 4/81 (4%) Frame = +1 Query: 22 DLTNQIKLGAATAGLVYDNVNRH--GATLTNTHIPGIGDKLSVAGKVNLFHNNDHDLSAK 195 D ++ ++G A G YD+ RH G + TNT +++ N+ +N+ ++ K Sbjct: 347 DFPDKPEMGKAKPGCSYDSYERHTLGISETNTEEDFCVSSMALDELNNITRDNNKEIGLK 406 Query: 196 AFATRNMPTISH--LPSTNTV 252 R M LPS ++ Sbjct: 407 LRRGRRMKNFQKEILPSLTSL 427 >At5g10490.1 68418.m01215 mechanosensitive ion channel domain-containing protein / MS ion channel domain-containing protein contains Pfam profile PF00924: Mechanosensitive ion channel Length = 519 Score = 27.1 bits (57), Expect = 7.4 Identities = 11/39 (28%), Positives = 21/39 (53%) Frame = +3 Query: 174 RP*FIREGVRDQKYAHHFPFTINQHCWWWSRIYVQGQDR 290 RP + E ++ + + T+ +H WWS ++G+DR Sbjct: 142 RPFVLNEWIQTKIEGYEVSGTV-EHVGWWSPTIIRGEDR 179 >At5g01240.2 68418.m00032 amino acid permease, putative strong similarity to AUX1 GI:1531758 from [Arabidopsis thaliana]; contains Pfam profile PF01490: Transmembrane amino acid transporter protein Length = 408 Score = 27.1 bits (57), Expect = 7.4 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +1 Query: 352 KLNLFKTPSTSLDFTAGWHKFDTPFMKSSWEPSTWFSL 465 KL L+ T +T++ +T G H M + W+P + S+ Sbjct: 157 KLVLYFTGATNILYTFGGHAVTVEIMHAMWKPRKFKSI 194 >At5g01240.1 68418.m00031 amino acid permease, putative strong similarity to AUX1 GI:1531758 from [Arabidopsis thaliana]; contains Pfam profile PF01490: Transmembrane amino acid transporter protein Length = 488 Score = 27.1 bits (57), Expect = 7.4 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +1 Query: 352 KLNLFKTPSTSLDFTAGWHKFDTPFMKSSWEPSTWFSL 465 KL L+ T +T++ +T G H M + W+P + S+ Sbjct: 237 KLVLYFTGATNILYTFGGHAVTVEIMHAMWKPRKFKSI 274 >At1g77550.1 68414.m09030 tubulin-tyrosine ligase family protein contains tubulin-tyrosine ligase family domain, Pfam:PF03133 Length = 867 Score = 27.1 bits (57), Expect = 7.4 Identities = 11/27 (40%), Positives = 18/27 (66%) Frame = +1 Query: 154 VNLFHNNDHDLSAKAFATRNMPTISHL 234 ++L + + H+L KAF+ MP +SHL Sbjct: 281 LDLSNRSIHNLVNKAFSVHEMPLLSHL 307 >At5g41730.1 68418.m05074 protein kinase family protein contains Pfam PF00069: Protein kinase domain Length = 711 Score = 26.6 bits (56), Expect = 9.8 Identities = 20/62 (32%), Positives = 30/62 (48%) Frame = +2 Query: 275 SRTRSVHQRAPLTLISLTRMTXIWAAN*TYSRLHPRRLTSLPAGTSSIRPS*SPLGNPAL 454 S TRSV+ AP+ +S + + A L +L S AGTS+++ SP +P Sbjct: 614 SDTRSVYSEAPMKKVSALKKSGEMAKLRRSPSLGSEKLRS--AGTSTVKARSSPKVSPLS 671 Query: 455 GF 460 F Sbjct: 672 PF 673 >At5g11980.1 68418.m01401 conserved oligomeric Golgi complex component-related / COG complex component-related similar to SP|Q96MW5 Conserved oligomeric Golgi complex component 8 {Homo sapiens}; contains Pfam profile PF04124: Dor1-like family Length = 569 Score = 26.6 bits (56), Expect = 9.8 Identities = 11/39 (28%), Positives = 20/39 (51%) Frame = +2 Query: 200 SRPEICPPFPIYHQPTLLVVVSNICSRTRSVHQRAPLTL 316 S+ ++ PP + P L V ++ + S + APL+L Sbjct: 390 SKDDVTPPSYLMEHPPLAVFINGVSSALNELRPCAPLSL 428 >At3g49490.1 68416.m05409 expressed protein Length = 953 Score = 26.6 bits (56), Expect = 9.8 Identities = 16/45 (35%), Positives = 27/45 (60%) Frame = -3 Query: 446 GSQEDFMKGVSNLCQPAVKSSDVDGVLNRFSLPPRX*SFLLKKSV 312 G QED ++G ++ +SSDV V++RF + R + +KKS+ Sbjct: 847 GDQEDKLEG--SVMANMGRSSDVSDVMDRFQILKRREAEQVKKSL 889 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,618,146 Number of Sequences: 28952 Number of extensions: 250019 Number of successful extensions: 668 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 655 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 668 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 927799552 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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