Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0001_I09
         (522 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g42740.1 68418.m05205 glucose-6-phosphate isomerase, cytosoli...   142   1e-34
At4g24620.1 68417.m03526 glucose-6-phosphate isomerase, putative...    62   2e-10
At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi...    29   1.4  
At4g34840.1 68417.m04943 nucleosidase-related contains weak simi...    29   1.9  
At4g24860.1 68417.m03559 AAA-type ATPase family protein contains...    29   1.9  
At1g10110.1 68414.m01140 F-box family protein contains Pfam PF00...    28   4.4  
At4g10070.1 68417.m01647 KH domain-containing protein DNA-direct...    27   5.8  
At2g04890.1 68415.m00507 scarecrow-like transcription factor 21 ...    27   5.8  
At4g32440.2 68417.m04618 agenet domain-containing protein contai...    27   7.7  
At4g32440.1 68417.m04617 agenet domain-containing protein contai...    27   7.7  
At3g50330.1 68416.m05504 basic helix-loop-helix (bHLH) family pr...    27   7.7  
At1g47128.1 68414.m05222 cysteine proteinase (RD21A) / thiol pro...    27   7.7  

>At5g42740.1 68418.m05205 glucose-6-phosphate isomerase, cytosolic
           (PGIC) identical to SP|P34795 Glucose-6-phosphate
           isomerase, cytosolic (EC 5.3.1.9) (GPI) (Phosphoglucose
           isomerase) (PGI) (Phosphohexose isomerase) (PHI)
           {Arabidopsis thaliana}; contains Pfam profile PF00342:
           glucose-6-phosphate isomerase
          Length = 560

 Score =  142 bits (344), Expect = 1e-34
 Identities = 65/123 (52%), Positives = 85/123 (69%)
 Frame = +3

Query: 123 ENLLNGANYMDEHFTTAPFERNAPVILALLGVWYHNFYGAETHALLPYDQYLHRYAAYFQ 302
           E  L GA+ +D+HF + PFE+N PV+L LL VW  +F G    A+LPY Q L ++A + Q
Sbjct: 297 EKFLKGASSIDQHFQSTPFEKNIPVLLGLLSVWNVSFLGYPARAILPYSQALEKFAPHIQ 356

Query: 303 QGDMESNGKYVTRAGDEVPYSTGPIVWGEPGTNGQHAFYQLIHPGTRLIPCDFIARAQTH 482
           Q  MESNGK V+  G  +P+ TG I +GEPGTNGQH+FYQLIH G R+IPCDFI   ++ 
Sbjct: 357 QVSMESNGKGVSIDGLPLPFETGEIDFGEPGTNGQHSFYQLIHQG-RVIPCDFIGIVKSQ 415

Query: 483 NPI 491
            P+
Sbjct: 416 QPV 418


>At4g24620.1 68417.m03526 glucose-6-phosphate isomerase, putative
           similar to glucose-6-phosphate isomerase [Spinacia
           oleracea] GI:3413511; contains Pfam profile PF00342:
           glucose-6-phosphate isomerase
          Length = 613

 Score = 62.5 bits (145), Expect = 2e-10
 Identities = 38/112 (33%), Positives = 53/112 (47%)
 Frame = +3

Query: 129 LLNGANYMDEHFTTAPFERNAPVILALLGVWYHNFYGAETHALLPYDQYLHRYAAYFQQG 308
           +L GA  MDE   T   + N   +LA+   W  N  G++   +LPY   L  ++ Y QQ 
Sbjct: 330 MLTGAALMDEATRTTSIKNNPAALLAMCWYWASNGVGSKDMVVLPYKDSLLLFSRYLQQL 389

Query: 309 DMESNGKYVTRAGDEVPYSTGPIVWGEPGTNGQHAFYQLIHPGTRLIPCDFI 464
            MES GK     G+ V  + G  V+G  G+  QHA+ Q +  G       FI
Sbjct: 390 VMESLGKEFDLDGNTV--NQGLTVYGNKGSTDQHAYIQQLRDGVHNFFATFI 439


>At2g39930.1 68415.m04907 isoamylase, putative / starch debranching
           enzyme, putative similar to isoamylase from [Solanum
           tuberosum] GI:27728145, [Triticum aestivum] GI:17932898,
           [Hordeum vulgare] GI:21314275, [Oryza sativa]
           GI:3252794; contains Pfam profiles PF00128: Alpha
           amylase catalytic domain, PF02922: Isoamylase N-terminal
           domain
          Length = 783

 Score = 29.5 bits (63), Expect = 1.4
 Identities = 15/36 (41%), Positives = 21/36 (58%), Gaps = 3/36 (8%)
 Frame = -1

Query: 354 PRLQLWSHISRYFPYRLVGSTRR-IYV--NTGHMVT 256
           P +  WS  SR+  + LV S ++ IYV  NT H+ T
Sbjct: 680 PEIPNWSETSRFVAFSLVDSVKKEIYVAFNTSHLAT 715


>At4g34840.1 68417.m04943 nucleosidase-related contains weak
           similarity to MTA/SAH nucleosidase (Swiss-Prot:O51931)
           [Buchnera aphidicola]
          Length = 254

 Score = 29.1 bits (62), Expect = 1.9
 Identities = 13/30 (43%), Positives = 17/30 (56%)
 Frame = -3

Query: 394 PGSPHTMGPVEYGTSSPALVTYFPLLSISP 305
           PG   T+G    GT   +LVTY  +L+I P
Sbjct: 66  PGKDSTLGVESVGTVPASLVTYASILAIQP 95


>At4g24860.1 68417.m03559 AAA-type ATPase family protein contains
           Pfam profile PF00004: ATPase, AAA family
          Length = 1122

 Score = 29.1 bits (62), Expect = 1.9
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = +3

Query: 315 ESNGKYVTRAGDEVPYSTGPIVWGEPGT 398
           E N  +V  +GDEV Y   PIV  +PG+
Sbjct: 174 EGNINHVLNSGDEVVYQQMPIVAAKPGS 201


>At1g10110.1 68414.m01140 F-box family protein contains Pfam
           PF00646: F-box domain; similar to  F-box protein family,
           AtFBX7 (GI:20197899) [Arabidopsis thaliana]
          Length = 332

 Score = 27.9 bits (59), Expect = 4.4
 Identities = 12/36 (33%), Positives = 16/36 (44%)
 Frame = -1

Query: 420 DRKRAGR*FQARPILWVPSSTGPRLQLWSHISRYFP 313
           DR R  R      +LWV   T   L +WS+   + P
Sbjct: 79  DRHRTSRAIVGNSVLWVDEKTKDYLVVWSYKEAFVP 114


>At4g10070.1 68417.m01647 KH domain-containing protein DNA-directed
           RNA polymerase (EC 2.7.7.6) II largestchain - mouse,
           PIR2:A28490
          Length = 725

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 22/83 (26%), Positives = 30/83 (36%), Gaps = 2/83 (2%)
 Frame = +3

Query: 201 LALLGVWYHNFYGAETHALLPYDQ--YLHRYAAYFQQGDMESNGKYVTRAGDEVPYSTGP 374
           L+ +   Y   +G E     PY Q  Y   Y   ++Q   +SN       G   P     
Sbjct: 461 LSQVSYGYGQSHGPEYGHAAPYSQTGYQQTYGQTYEQPKYDSNPPMQPPYGGSYP----- 515

Query: 375 IVWGEPGTNGQHAFYQLIHPGTR 443
                P   GQ  +YQ+  PG R
Sbjct: 516 -----PAGGGQSGYYQMQQPGVR 533


>At2g04890.1 68415.m00507 scarecrow-like transcription factor 21
           (SCL21)
          Length = 413

 Score = 27.5 bits (58), Expect = 5.8
 Identities = 13/37 (35%), Positives = 17/37 (45%)
 Frame = +3

Query: 243 ETHALLPYDQYLHRYAAYFQQGDMESNGKYVTRAGDE 353
           E++  L Y   LH    YF+ G M +NG       DE
Sbjct: 119 ESYEFLSYVYVLHEVCPYFKFGYMSANGAIAEAMKDE 155


>At4g32440.2 68417.m04618 agenet domain-containing protein contains
           Pfam PF05641: Agenet domain
          Length = 393

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 13/34 (38%), Positives = 22/34 (64%)
 Frame = -2

Query: 398 SSRLAPYYGSRRVRDLVSSSGHIFPVTFHIALLE 297
           S++ APY G+ R  ++VS +GH + V F+   +E
Sbjct: 13  SNKEAPY-GAWRCAEIVSGNGHTYNVRFYSFQIE 45


>At4g32440.1 68417.m04617 agenet domain-containing protein contains
           Pfam PF05641: Agenet domain
          Length = 377

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 13/34 (38%), Positives = 22/34 (64%)
 Frame = -2

Query: 398 SSRLAPYYGSRRVRDLVSSSGHIFPVTFHIALLE 297
           S++ APY G+ R  ++VS +GH + V F+   +E
Sbjct: 13  SNKEAPY-GAWRCAEIVSGNGHTYNVRFYSFQIE 45


>At3g50330.1 68416.m05504 basic helix-loop-helix (bHLH) family
           protein contains Pfam profile: PF00010 helix-loop-helix
           DNA-binding domain
          Length = 231

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 19/68 (27%), Positives = 30/68 (44%), Gaps = 2/68 (2%)
 Frame = +3

Query: 150 MDEHFTTAPFERNAPVILALLGVWYHNFYGAETHALLPYDQYL--HRYAAYFQQGDMESN 323
           + EHF+ +    N   I+ L     H F+   TH+ LP+DQ +  H+    F+     S+
Sbjct: 18  LPEHFSNSNPNPNPHNIMMLSESNTHPFFFNPTHSHLPFDQTMPHHQPGLNFRYAPSPSS 77

Query: 324 GKYVTRAG 347
                R G
Sbjct: 78  SLPEKRGG 85


>At1g47128.1 68414.m05222 cysteine proteinase (RD21A) / thiol
           protease identical to SP|P43297 Cysteine proteinase
           RD21A precursor (EC 3.4.22.-) {Arabidopsis thaliana},
           thiol protease RD21A SP:P43297 from [Arabidopsis
           thaliana]
          Length = 462

 Score = 27.1 bits (57), Expect = 7.7
 Identities = 18/55 (32%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
 Frame = +3

Query: 237 GAETHALLPYDQYLHRY--AAYFQQGDMESNGKYVTRAGDEVPYSTGPIVWGEPG 395
           G    A L  D+Y  +Y  A   ++G+  ++ +Y  R GDE+P S   I W + G
Sbjct: 96  GLTRFADLTNDEYRSKYLGAKMEKKGERRTSLRYEARVGDELPES---IDWRKKG 147


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,348,184
Number of Sequences: 28952
Number of extensions: 217478
Number of successful extensions: 555
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 544
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 553
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)