BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I09A02NGRL0001_I02 (625 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23G3.05c |||regulator of G-protein signaling |Schizosaccharo... 33 0.044 SPAC13G7.07 |arb2||argonaute binding protein 2|Schizosaccharomyc... 32 0.077 SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pomb... 28 0.95 SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyc... 27 2.2 SPAC29B12.02c |set2||histone lysine methyltransferase Set2 |Schi... 27 2.9 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 26 3.8 SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 3.8 SPBC1778.04 |spo6||Spo4-Spo6 kinase complex regulatory subunit S... 26 3.8 SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr 1||... 26 5.1 SPCC594.04c |||steroid oxidoreductase superfamily protein|Schizo... 26 5.1 SPBC16H5.09c |||alpha-1,2-mannosyltransferase |Schizosaccharomyc... 25 8.9 SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharo... 25 8.9 >SPAC23G3.05c |||regulator of G-protein signaling |Schizosaccharomyces pombe|chr 1|||Manual Length = 343 Score = 32.7 bits (71), Expect = 0.044 Identities = 13/33 (39%), Positives = 19/33 (57%) Frame = +2 Query: 347 GPHRYVISGDWTTSLRDISFMYWPKRGTLRIQE 445 GPH + + SL D+S +YWP G L ++E Sbjct: 77 GPHHFTRFRSASLSLEDLSTVYWPNFGPLLLEE 109 >SPAC13G7.07 |arb2||argonaute binding protein 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 266 Score = 31.9 bits (69), Expect = 0.077 Identities = 14/57 (24%), Positives = 29/57 (50%) Frame = +3 Query: 174 YCYDGYARDENNVCIPVEQCKNPAIKQKRHVKEGTFRCAPANSLESQCTSRLSTFII 344 Y + +EN + I ++ +P I R+++EG R A + ++CT + + +I Sbjct: 94 YATRNWQTNENGIVIIIQSMSHPLIWSNRNLREGDVRRATVLDVIAKCTEKKLSVVI 150 >SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pombe|chr 2|||Manual Length = 440 Score = 28.3 bits (60), Expect = 0.95 Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 1/46 (2%) Frame = +2 Query: 359 YVISGDWTTSLRDISF-MYWPKRGTLRIQENMSPRAVLQFSSKIQM 493 YV+SG WT SL++ F Y K L + +N Q +S I M Sbjct: 269 YVLSGGWTVSLQEFVFNEYCVKSSRLALHDNKDIEYSQQPASAIFM 314 >SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 2|||Manual Length = 807 Score = 27.1 bits (57), Expect = 2.2 Identities = 10/31 (32%), Positives = 15/31 (48%) Frame = +3 Query: 375 TGPHRYVISVSCTGPNEEHYASKKTCPPELC 467 TG H + + +S GP+ + TC LC Sbjct: 750 TGAHNFQLRISVLGPDSDQLPISHTCFNHLC 780 >SPAC29B12.02c |set2||histone lysine methyltransferase Set2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 798 Score = 26.6 bits (56), Expect = 2.9 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = +3 Query: 48 IECTGKNEFYMCGPACDNVCSELHE 122 IECT ++ +CGP+C N + HE Sbjct: 156 IECTDEDN--VCGPSCQNQRFQRHE 178 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 26.2 bits (55), Expect = 3.8 Identities = 11/35 (31%), Positives = 16/35 (45%) Frame = +3 Query: 486 FKCDAXETPKSGCFCKPGYLREKDNTPCIPXCKCP 590 +KC E P+ GC+ Y K++ P C P Sbjct: 71 YKCSNEECPRPGCY--RSYSSNKEDHPPCEICNSP 103 >SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1233 Score = 26.2 bits (55), Expect = 3.8 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = -3 Query: 122 FMQFRADIITGGAAHVKLIFTGTFYWIIKIAL 27 F++F IT + H +IFT ++KIAL Sbjct: 708 FIEFLFHNITVSSKHTAVIFTSDLLMVLKIAL 739 >SPBC1778.04 |spo6||Spo4-Spo6 kinase complex regulatory subunit Spo6|Schizosaccharomyces pombe|chr 2|||Manual Length = 474 Score = 26.2 bits (55), Expect = 3.8 Identities = 15/50 (30%), Positives = 25/50 (50%), Gaps = 2/50 (4%) Frame = +3 Query: 27 KRNFDDPIEC-TGKNEFYMCGPA-CDNVCSELHEQNRTHCPILNIMCNRK 170 KR+ +P+ T ++ +C A C CS+ + Q +TH P N + K Sbjct: 31 KRDIQNPLSYKTETSDKELCQTAACATSCSDWYPQQQTHMPHQNAFDSAK 80 >SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr 1|||Manual Length = 113 Score = 25.8 bits (54), Expect = 5.1 Identities = 13/38 (34%), Positives = 18/38 (47%) Frame = +3 Query: 78 MCGPACDNVCSELHEQNRTHCPILNIMCNRKCYCYDGY 191 +CG AC N + ++ HC N N KC + GY Sbjct: 72 ICG-ACKNSLTVEEYRSTVHCKYCNHPFNPKCKNHAGY 108 >SPCC594.04c |||steroid oxidoreductase superfamily protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 338 Score = 25.8 bits (54), Expect = 5.1 Identities = 15/54 (27%), Positives = 21/54 (38%) Frame = -1 Query: 352 WSRMMNVLSLEVHWLSSEFAGAHLKVPSLTWRFCLMAGFLHCSTGMHTLFSSLA 191 WSR N L+ ++ WLS GA L W G + G L ++ Sbjct: 247 WSRHPNFLAEQLIWLSFYLFGAIASESLLNWTIFAWLGLVGVFQGSTRLTEKMS 300 >SPBC16H5.09c |||alpha-1,2-mannosyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 372 Score = 25.0 bits (52), Expect = 8.9 Identities = 10/22 (45%), Positives = 13/22 (59%), Gaps = 1/22 (4%) Frame = +3 Query: 510 PKSG-CFCKPGYLREKDNTPCI 572 P+ G CFCKP + N+ CI Sbjct: 337 PRKGRCFCKPEEIDLSSNSSCI 358 >SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 25.0 bits (52), Expect = 8.9 Identities = 12/19 (63%), Positives = 13/19 (68%) Frame = -3 Query: 542 ISRLTEASRLGGFVSVAFE 486 +S LTEA L GFVSV E Sbjct: 437 LSELTEAQELLGFVSVIIE 455 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,808,928 Number of Sequences: 5004 Number of extensions: 61467 Number of successful extensions: 158 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 154 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 158 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 275671126 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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