BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_H23
(542 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 30 0.19
SPBC4F6.16c |ero11||ER oxidoreductin Ero1a|Schizosaccharomyces p... 27 2.4
SPCC4G3.08 |psk1||serine/threonine protein kinase Psk1|Schizosac... 26 3.1
SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ... 25 5.5
SPAC3H1.13 |ppk13||serine/threonine protein kinase Ppk13 |Schizo... 25 7.2
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 25 7.2
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 25 9.5
SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon... 25 9.5
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 30.3 bits (65), Expect = 0.19
Identities = 17/54 (31%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
Frame = +3
Query: 357 SAHYLFYSLIFMNVAPNLLVL-TPIFLFALLHAASYSLTILDTLGQNSMWVARL 515
SA +Y I ++ PN+ L TP ++A A + LT +LG + +W++R+
Sbjct: 2014 SASLFWY--ILHSLVPNIYALSTPFIIYATAFALA-GLTTFSSLGDSRLWISRV 2064
>SPBC4F6.16c |ero11||ER oxidoreductin Ero1a|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 467
Score = 26.6 bits (56), Expect = 2.4
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = +3
Query: 315 SISREFLARFFLEDSAHYLFYSLIFMNVAPNLL 413
S S+E LA FF+ D YLFY+L F+ P ++
Sbjct: 437 SPSKEILA-FFI-DQTWYLFYALFFICNVPRVI 467
>SPCC4G3.08 |psk1||serine/threonine protein kinase
Psk1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 436
Score = 26.2 bits (55), Expect = 3.1
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Frame = +3
Query: 75 QNPQS--GDTGPPKGIPALKAHVIANKID 155
+NP+ G GP KG A+K H I +ID
Sbjct: 326 KNPKQRLGADGPEKGYDAIKKHRIYRRID 354
>SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster
type |Schizosaccharomyces pombe|chr 2|||Manual
Length = 827
Score = 25.4 bits (53), Expect = 5.5
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +2
Query: 170 NPSANSPLHYRISYTYF*QSSVS 238
+PSAN P H Y Y+ +SS S
Sbjct: 264 SPSANYPYHEAYEYYYYIRSSFS 286
>SPAC3H1.13 |ppk13||serine/threonine protein kinase Ppk13
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 344
Score = 25.0 bits (52), Expect = 7.2
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = -3
Query: 189 GLLALGLPRALHQFC*Q*HELLMLVCPLVGLCLQIEGSDQP 67
G LAL + A + F + H L+C +V CLQ E + +P
Sbjct: 290 GSLALAVCNAQYSFPRK-HPYSTLLCEIVEACLQREPNKRP 329
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 25.0 bits (52), Expect = 7.2
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +3
Query: 87 SGDTGPPKGIPALKAHVIA 143
SG TG PKG+ L ++IA
Sbjct: 254 SGSTGKPKGVVLLHRNIIA 272
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 24.6 bits (51), Expect = 9.5
Identities = 16/51 (31%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = -2
Query: 469 VRE*EAACRSANKNIGVKTNKFGATFMKINE*N-R*CALSSKKNRARNSLE 320
+RE E + ++ N N+G K N K++E + R L S+ N+ +N +E
Sbjct: 780 IREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQLNQDKNQIE 830
>SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 678
Score = 24.6 bits (51), Expect = 9.5
Identities = 10/36 (27%), Positives = 19/36 (52%)
Frame = +2
Query: 326 RVSCSIFLGGQRTLPVLLINLHERGTKFIGFDADIF 433
R S+F+ ++T + +ERG ++ D +IF
Sbjct: 171 RTESSVFVIDKKTSQCVHYQANERGKHYVSMDPEIF 206
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,291,100
Number of Sequences: 5004
Number of extensions: 46612
Number of successful extensions: 130
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 130
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 223909422
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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