BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I09A02NGRL0001_H15 (608 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 90 3e-19 SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 57 2e-09 SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 51 1e-07 SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 42 9e-05 SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 40 3e-04 SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 38 0.002 SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1... 28 1.2 SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 27 2.1 SPAC25B8.16 |||RNase P and RNase MRP subunit |Schizosaccharomyce... 26 5.0 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 5.0 SPBC56F2.08c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 26 5.0 SPBC3H7.02 |||sulfate transporter |Schizosaccharomyces pombe|chr... 25 6.5 SPCC1682.13 |||SWIRM domain protein|Schizosaccharomyces pombe|ch... 25 6.5 SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S... 25 8.7 >SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 89.8 bits (213), Expect = 3e-19 Identities = 40/98 (40%), Positives = 63/98 (64%) Frame = +3 Query: 3 GYDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRAL 182 GYD+ GPH+ + PS + ++G+RSQSARTY+E++L TF D +EL+ LRAL Sbjct: 136 GYDESGPHLLEFQPSGLVLEYLGTSMGSRSQSARTYIERNLDTFPDSSREELILSALRAL 195 Query: 183 RDTLPNEMDLNNKNVSIAIVGPDTPLRVCEEEELGRFL 296 RDTL + +L +NVSI+++G D + ++ + +L Sbjct: 196 RDTLSKDQELTEENVSISVIGKDEKYTLYDQNDTKEWL 233 >SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 245 Score = 56.8 bits (131), Expect = 2e-09 Identities = 30/80 (37%), Positives = 48/80 (60%) Frame = +3 Query: 3 GYDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRAL 182 G D++GP +YQ PS +F +A AIG S +A+T+LEK + + +L + V + AL Sbjct: 138 GMDEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYND--ELELDDAVHTAILAL 195 Query: 183 RDTLPNEMDLNNKNVSIAIV 242 ++T E +L N+ IA+V Sbjct: 196 KETF--EGELTEDNIEIAVV 213 >SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 51.2 bits (117), Expect = 1e-07 Identities = 31/99 (31%), Positives = 51/99 (51%) Frame = +3 Query: 3 GYDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRAL 182 G D+ GP +Y + PS +F A AIG+ S+ A++ L K D L+E L+ L Sbjct: 146 GIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHK--DMTLEEAEVLILKVL 203 Query: 183 RDTLPNEMDLNNKNVSIAIVGPDTPLRVCEEEELGRFLA 299 R + ++D +KNV +A V + + +EE+ +A Sbjct: 204 RQVMEEKLD--SKNVQLAKVTAEGGFHIYNDEEMADAVA 240 >SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 41.5 bits (93), Expect = 9e-05 Identities = 25/93 (26%), Positives = 44/93 (47%), Gaps = 1/93 (1%) Frame = +3 Query: 9 DDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHL-STFIDCDLQELVAHGLRALR 185 D+ GP +++ P+ + +A + G + +LEK I +L + V G++AL Sbjct: 143 DEIGPSLFKLDPAGFYIGYKATSAGPKQTETINWLEKRFKKDGIPSNLTDTVETGIQALM 202 Query: 186 DTLPNEMDLNNKNVSIAIVGPDTPLRVCEEEEL 284 +L D + + I +V D P RV EE+ Sbjct: 203 SSL--STDFKSTELQIGVVEDDKPFRVLSVEEI 233 >SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 39.9 bits (89), Expect = 3e-04 Identities = 27/107 (25%), Positives = 53/107 (49%), Gaps = 2/107 (1%) Frame = +3 Query: 3 GYD--DQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLR 176 G+D D P +YQT P+ + +A AIG S++AR YLEK+ + D E + + Sbjct: 136 GFDVGDNTPRVYQTEPAGIYNAWKATAIGRASKAAREYLEKNWKEGLSRD--ETIHLAVS 193 Query: 177 ALRDTLPNEMDLNNKNVSIAIVGPDTPLRVCEEEELGRFLALVEGEE 317 +L + + + N+ +AI+ P + +++ + ++ E+ Sbjct: 194 SLLEVVQTA----SGNIELAIMDPGKDVEFLHSDKIDTIVKEIQDEK 236 >SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharomyces pombe|chr 3|||Manual Length = 253 Score = 37.5 bits (83), Expect = 0.002 Identities = 27/85 (31%), Positives = 38/85 (44%), Gaps = 2/85 (2%) Frame = +3 Query: 6 YDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRALR 185 YD +GPH+Y P+ ++ A G Q AR LEK F +++ V R L Sbjct: 140 YDSEGPHLYMVEPNGVYWGYNGAAAGKGRQVARNELEK--LNFSSLKMKDAVKEAARILY 197 Query: 186 DTLPNEMDLNNK--NVSIAIVGPDT 254 T E NNK + + VG +T Sbjct: 198 ATHDEE---NNKEHEIEMTWVGVET 219 >SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1023 Score = 27.9 bits (59), Expect = 1.2 Identities = 14/30 (46%), Positives = 20/30 (66%) Frame = -2 Query: 289 LPSSSSSQTRKGVSGPTIAIDTFLLLRSIS 200 +PSSSS+++R G P+I D+ L L S S Sbjct: 103 IPSSSSNRSRGGPYTPSIRDDSLLALLSFS 132 >SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 267 Score = 27.1 bits (57), Expect = 2.1 Identities = 17/60 (28%), Positives = 29/60 (48%), Gaps = 1/60 (1%) Frame = +3 Query: 3 GYDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLE-KHLSTFIDCDLQELVAHGLRA 179 GYD +GPH++ + +A+G+ S +A + LE K+ + ELV + A Sbjct: 137 GYDCKGPHLFTIAAHGSSDKLPYVALGSGSLAAISVLETKYQPDLERHEAMELVKEAIEA 196 >SPAC25B8.16 |||RNase P and RNase MRP subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 698 Score = 25.8 bits (54), Expect = 5.0 Identities = 9/29 (31%), Positives = 18/29 (62%) Frame = -1 Query: 101 GGLRARTNRHCATVEKVSRWTCLIYVRSL 15 G L+ R +C + +++S+W +IY +L Sbjct: 598 GSLQDRARIYCLSDDELSKWKTIIYKENL 626 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 25.8 bits (54), Expect = 5.0 Identities = 11/25 (44%), Positives = 12/25 (48%) Frame = -3 Query: 315 PHPRPEPGTCRVPPPRKHVRVYPDP 241 P PR P T R PPP R +P Sbjct: 364 PPPRSAPSTGRQPPPLSSSRAVSNP 388 >SPBC56F2.08c |||RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 661 Score = 25.8 bits (54), Expect = 5.0 Identities = 12/36 (33%), Positives = 21/36 (58%) Frame = -2 Query: 289 LPSSSSSQTRKGVSGPTIAIDTFLLLRSISFGSVSL 182 + S S S + G+S P+ ++D+ + FGS+SL Sbjct: 480 MTSKSISPSLSGISAPSASVDSSASRLARDFGSLSL 515 >SPBC3H7.02 |||sulfate transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 877 Score = 25.4 bits (53), Expect = 6.5 Identities = 12/23 (52%), Positives = 13/23 (56%) Frame = -3 Query: 309 PRPEPGTCRVPPPRKHVRVYPDP 241 P PG VPPP V VYP+P Sbjct: 23 PDSVPGL--VPPPSNFVPVYPEP 43 >SPCC1682.13 |||SWIRM domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 272 Score = 25.4 bits (53), Expect = 6.5 Identities = 10/17 (58%), Positives = 10/17 (58%) Frame = +1 Query: 247 RIHPYVFARRRNSAGSW 297 RIHP ARR N G W Sbjct: 74 RIHPIPKARRNNHVGRW 90 >SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 1052 Score = 25.0 bits (52), Expect = 8.7 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 4/41 (9%) Frame = +2 Query: 461 ILPSSEVPLLPSNIIFYTVIIF----KWVLPSTIHFHNKEM 571 +LP + PS+++FY V++ K VL S H+KE+ Sbjct: 649 LLPKDLANIQPSDLLFYAVLLVLINGKLVLLSLKKQHSKEL 689 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,587,645 Number of Sequences: 5004 Number of extensions: 53423 Number of successful extensions: 148 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 137 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 146 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 268287866 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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