Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_H15
(608 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1) ... 85 3e-17
At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2) ... 83 1e-16
At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putativ... 69 2e-12
At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1) (... 69 2e-12
At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1) ... 53 2e-07
At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2) ... 52 2e-07
At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2) ... 51 7e-07
At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1) 47 8e-06
At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2) ... 44 8e-05
At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1) ... 42 3e-04
At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1) (... 33 0.11
At3g19090.1 68416.m02426 RNA-binding protein, putative similar t... 29 3.2
At2g47850.1 68415.m05972 zinc finger (CCCH-type) family protein ... 29 3.2
At1g20130.1 68414.m02518 family II extracellular lipase, putativ... 29 3.2
At1g49750.1 68414.m05579 leucine-rich repeat family protein cont... 28 4.2
At2g30170.1 68415.m03671 expressed protein 27 7.4
At3g43760.1 68416.m04675 hypothetical protein 27 9.7
>At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1)
(gb|AAC32062.1)
Length = 278
Score = 85.0 bits (201), Expect = 3e-17
Identities = 38/102 (37%), Positives = 63/102 (61%)
Frame = +3
Query: 3 GYDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRAL 182
G D+ G H+Y CPS N+F+ +A AIG+RSQ+A+TYLE+ +F D ++L+ + A+
Sbjct: 136 GLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFESFGDSSREDLIKDAILAV 195
Query: 183 RDTLPNEMDLNNKNVSIAIVGPDTPLRVCEEEELGRFLALVE 308
R+TL E L + ++AI+G D P ++E + + + E
Sbjct: 196 RETLQGE-TLKSSLCTVAILGVDEPFHFLDQEAIQKVIDTFE 236
>At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2)
(PRC2B) (PRS1) identical to GB:AAC32063 from
[Arabidopsis thaliana] (Genetics 149 (2), 677-692
(1998)); identical to cDNA proteasome subunit prc2b
GI:2511585
Length = 277
Score = 83.0 bits (196), Expect = 1e-16
Identities = 35/102 (34%), Positives = 63/102 (61%)
Frame = +3
Query: 3 GYDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRAL 182
G D+ G H+Y CPS N+F+ +A AIG+RSQ+A+TYLE+ +F + ++L+ + A+
Sbjct: 136 GLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERKFESFQESSKEDLIKDAIMAI 195
Query: 183 RDTLPNEMDLNNKNVSIAIVGPDTPLRVCEEEELGRFLALVE 308
R+TL E L + +++++G D P ++E + + + E
Sbjct: 196 RETLQGE-TLKSSLCTVSVLGVDEPFHFLDQESIQKVIDTFE 236
>At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putative
nearly identical to SP|O23708 Proteasome subunit alpha
type 2 (EC 3.4.25.1) (20S proteasome alpha subunit B)
{Arabidopsis thaliana} and to At1g16470
Length = 235
Score = 68.9 bits (161), Expect = 2e-12
Identities = 35/102 (34%), Positives = 59/102 (57%)
Frame = +3
Query: 3 GYDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRAL 182
GYDD+GP +YQ PS ++F +A A+G +A+T+LEK + D +L + + + L
Sbjct: 138 GYDDKGPQLYQVDPSGSYFSWKASAMGKNVSNAKTFLEKRYTE--DMELDDAIHTAILTL 195
Query: 183 RDTLPNEMDLNNKNVSIAIVGPDTPLRVCEEEELGRFLALVE 308
++ E ++++KN+ I +G D RV E+ +LA VE
Sbjct: 196 KEGF--EGEISSKNIEIGKIGTDKVFRVLTPAEIDDYLAEVE 235
>At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1)
(PRC3) identical to proteasome subunit alpha type 2
SP:O23708, GI:6093778; identical to cDNA proteasome
subunit prc3 GI:2511573
Length = 235
Score = 68.9 bits (161), Expect = 2e-12
Identities = 35/102 (34%), Positives = 59/102 (57%)
Frame = +3
Query: 3 GYDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRAL 182
GYDD+GP +YQ PS ++F +A A+G +A+T+LEK + D +L + + + L
Sbjct: 138 GYDDKGPQLYQVDPSGSYFSWKASAMGKNVSNAKTFLEKRYTE--DMELDDAIHTAILTL 195
Query: 183 RDTLPNEMDLNNKNVSIAIVGPDTPLRVCEEEELGRFLALVE 308
++ E ++++KN+ I +G D RV E+ +LA VE
Sbjct: 196 KEGF--EGEISSKNIEIGKIGADKVFRVLTPAEIDDYLAEVE 235
>At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1)
identical to 20S proteasome subunit PAE1 GI:3421087 from
[Arabidopsis thaliana]
Length = 237
Score = 52.8 bits (121), Expect = 2e-07
Identities = 32/93 (34%), Positives = 48/93 (51%)
Frame = +3
Query: 3 GYDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRAL 182
G+D+ GP +Y T PS F+ C A AIG+ S+ A + L++ + D LQE + L
Sbjct: 143 GHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNK--DLSLQEAETIAVSIL 200
Query: 183 RDTLPNEMDLNNKNVSIAIVGPDTPLRVCEEEE 281
+ + ++ N NV IA V P L +E E
Sbjct: 201 KQVMEEKVTPN--NVDIAKVAPAYHLYTPQEVE 231
>At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2)
identical to 20S proteasome subunit PAE2 GB:AAC32061
from [Arabidopsis thaliana]
Length = 237
Score = 52.4 bits (120), Expect = 2e-07
Identities = 32/93 (34%), Positives = 48/93 (51%)
Frame = +3
Query: 3 GYDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRAL 182
G+D+ GP +Y T PS F+ C A AIG+ S+ A + L++ + D LQE + L
Sbjct: 143 GHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNK--DITLQEAETIAVSIL 200
Query: 183 RDTLPNEMDLNNKNVSIAIVGPDTPLRVCEEEE 281
+ + ++ N NV IA V P L +E E
Sbjct: 201 KQVMEEKVTPN--NVDIAKVAPAYHLYTPQEVE 231
>At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2)
(PRS1) (PRC6) identical to SP|O24616 Proteasome subunit
alpha type 7-2 (EC 3.4.25.1) (20S proteasome alpha
subunit D2) {Arabidopsis thaliana}
Length = 250
Score = 50.8 bits (116), Expect = 7e-07
Identities = 33/100 (33%), Positives = 53/100 (53%), Gaps = 1/100 (1%)
Frame = +3
Query: 21 PHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRALRDTLPN 200
P +YQT PS F +A A G S S R +LEK+ + + QE + +RAL +
Sbjct: 144 PSLYQTDPSGTFSAWKANATGRNSNSIREFLEKN---YKESSGQETIKLAIRALLEV--- 197
Query: 201 EMDLNNKNVSIAIV-GPDTPLRVCEEEELGRFLALVEGEE 317
++ KN+ +A++ +T LR EE E+ +A +E E+
Sbjct: 198 -VESGGKNIEVAVMTREETGLRQLEEAEIDAIVAKIEAEK 236
>At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1)
Length = 250
Score = 47.2 bits (107), Expect = 8e-06
Identities = 33/100 (33%), Positives = 53/100 (53%), Gaps = 1/100 (1%)
Frame = +3
Query: 21 PHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRALRDTLPN 200
P +YQT PS F +A A G S S R +LEK+ + + QE V +RAL +
Sbjct: 144 PALYQTDPSGTFSAWKANATGRNSNSIREFLEKN---YKESAGQETVKLAIRALLEV--- 197
Query: 201 EMDLNNKNVSIAIV-GPDTPLRVCEEEELGRFLALVEGEE 317
++ KN+ +A++ + L+ EEEE+ +A +E E+
Sbjct: 198 -VESGGKNIEVAVMTREEGVLKQLEEEEIDIIVAEIEAEK 236
>At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2)
identical to GB:AF043519
Length = 246
Score = 44.0 bits (99), Expect = 8e-05
Identities = 26/100 (26%), Positives = 47/100 (47%), Gaps = 1/100 (1%)
Frame = +3
Query: 9 DDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRALRD 188
+++GP +Y+ P+ +F+ +A + G + Q A +LEK + E V + AL+
Sbjct: 145 EERGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEKKMKENPAFTYDETVQTAISALQS 204
Query: 189 TLPNEMDLNNKNVSIAIVGPDTPL-RVCEEEELGRFLALV 305
L + D + + +V D PL R EE+ L +
Sbjct: 205 VL--QEDFKATEIEVGVVRADDPLFRSLRTEEIDEHLTAI 242
>At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1)
(PRC1) identical to proteasome subunit alpha type 6-1
SP:O81146 GI:12643647 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc1 GI:2511587
Length = 246
Score = 41.9 bits (94), Expect = 3e-04
Identities = 24/100 (24%), Positives = 45/100 (45%), Gaps = 1/100 (1%)
Frame = +3
Query: 9 DDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRALRD 188
++ GP +Y+ P+ +F+ +A + G + Q A +LEK + E V + AL+
Sbjct: 145 EENGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEKKMKENPSFTFDETVQTAISALQS 204
Query: 189 TLPNEMDLNNKNVSIAIVGPDTP-LRVCEEEELGRFLALV 305
L + D + + +V + P R EE+ L +
Sbjct: 205 VL--QEDFKATEIEVGVVRAENPEFRALTTEEIEEHLTAI 242
>At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1)
(PRC8) identical to proteasome subunit alpha type 3
SP:O23715, GI:12644056 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc8 GI:2511591
Length = 249
Score = 33.5 bits (73), Expect = 0.11
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = +3
Query: 3 GYDDQGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEK 119
GYD GP +Y PS + AIG Q+A+T +EK
Sbjct: 140 GYDRDGPQLYMIEPSGISYRYFGAAIGKGKQAAKTEIEK 178
>At3g19090.1 68416.m02426 RNA-binding protein, putative similar to
RNA-binding protein homolog GB:AAF00075 GI:6449448 from
[Brassica napus]; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 455
Score = 28.7 bits (61), Expect = 3.2
Identities = 16/55 (29%), Positives = 24/55 (43%)
Frame = +3
Query: 117 KHLSTFIDCDLQELVAHGLRALRDTLPNEMDLNNKNVSIAIVGPDTPLRVCEEEE 281
K S F D D +EL G + + LP++ N +VG +R+C E
Sbjct: 220 KRTSQFTDRDREEL--QGRTVVAENLPDDHSYQNLEKIFGVVGNVKAIRICHPPE 272
>At2g47850.1 68415.m05972 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 468
Score = 28.7 bits (61), Expect = 3.2
Identities = 14/32 (43%), Positives = 17/32 (53%)
Frame = -3
Query: 312 HPRPEPGTCRVPPPRKHVRVYPDPQSLLTHFY 217
HP+P GT PPP + YP QSL+ Y
Sbjct: 161 HPQPA-GTTVPPPPASAPQFYPSVQSLMPDQY 191
>At1g20130.1 68414.m02518 family II extracellular lipase, putative
contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase; similar to EXL3 (PMID:11431566)
Length = 1006
Score = 28.7 bits (61), Expect = 3.2
Identities = 10/25 (40%), Positives = 12/25 (48%)
Frame = -3
Query: 315 PHPRPEPGTCRVPPPRKHVRVYPDP 241
P P P P C PPP+ + P P
Sbjct: 42 PPPAPSPSPCPSPPPKPQPKPVPPP 66
>At1g49750.1 68414.m05579 leucine-rich repeat family protein
contains leucine-rich repeats, Pfam:PF00560
Length = 494
Score = 28.3 bits (60), Expect = 4.2
Identities = 11/26 (42%), Positives = 12/26 (46%)
Frame = -3
Query: 318 APHPRPEPGTCRVPPPRKHVRVYPDP 241
+P P PEP C PPP P P
Sbjct: 51 SPEPEPEPADCPPPPPPPPCPPPPSP 76
>At2g30170.1 68415.m03671 expressed protein
Length = 298
Score = 27.5 bits (58), Expect = 7.4
Identities = 16/60 (26%), Positives = 29/60 (48%)
Frame = +3
Query: 15 QGPHIYQTCPSANFFDCRAMAIGARSQSARTYLEKHLSTFIDCDLQELVAHGLRALRDTL 194
+G I+ T P ++FDC + SA+TYL+ S ++ +++ G L D +
Sbjct: 163 EGQIIFATAPQEHYFDCPYQL--SSEGSAQTYLDASFS-IVEVQKGDVIVMGSDGLFDNV 219
>At3g43760.1 68416.m04675 hypothetical protein
Length = 280
Score = 27.1 bits (57), Expect = 9.7
Identities = 11/42 (26%), Positives = 21/42 (50%)
Frame = +1
Query: 187 THSQMKWILIIKMCQ*RLWVRIHPYVFARRRNSAGSWLWSRV 312
+H W+ I+ R ++ P+ + SAGSW+W ++
Sbjct: 44 SHGDSLWVKWIE----RFLLKKEPFWTVKENTSAGSWIWRKL 81
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,057,109
Number of Sequences: 28952
Number of extensions: 316678
Number of successful extensions: 1021
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 884
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1016
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1216725696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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