BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_H06
(500 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha... 27 1.6
SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces pomb... 25 4.8
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 25 6.4
SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|c... 25 6.4
SPAC22A12.11 |dak1||dihydroxyacetone kinase Dak1|Schizosaccharom... 25 8.4
SPAC22F3.12c |rgs1||regulator of G-protein signaling Rgs1|Schizo... 25 8.4
SPBC30B4.05 |kap109||karyopherin Kap109|Schizosaccharomyces pomb... 25 8.4
>SPAC13G7.04c |mac1||membrane anchored protein Mac1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 756
Score = 27.1 bits (57), Expect = 1.6
Identities = 17/52 (32%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +2
Query: 305 VNGHGATLTNTHIPG-FGDKLTAAGKVNLFHNDYHDLSAQAFATKNMPSIPQ 457
VN H + ++ I G +T A +V +N +DL AQA KN+ + P+
Sbjct: 440 VNDHSSFPSDLPIKGEMSTNMTGAPRVGSRNNSSNDLHAQAGMLKNVGNGPR 491
>SPBC1289.15 ||SPBC8E4.07c|glycoprotein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1283
Score = 25.4 bits (53), Expect = 4.8
Identities = 17/60 (28%), Positives = 27/60 (45%)
Frame = +2
Query: 170 DGTSGAIVKVPITGNENHKLSALGSVDLTNQMKLGAATAGLAYDNVNGHGATLTNTHIPG 349
D +SGA++ V T + GS+ T+ + T+G + V T+T T I G
Sbjct: 792 DTSSGAVIVVEPTAGTVTETIVSGSIPFTSTIPAQGTTSG-TVEVVEPTAGTVTETIISG 850
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.0 bits (52), Expect = 6.4
Identities = 16/60 (26%), Positives = 26/60 (43%)
Frame = -2
Query: 451 NAGHVLSSESLCAEVMVIIVEQVDFASGGQLVTETRDVSVCKSRSVSIDIVVCQSSCSGS 272
N+GH +S S+ + + + ++S + DVSV S S DIV S +
Sbjct: 139 NSGHASASTSIPSTAITVTANSTIYSSATSSFPYSTDVSV--STGTSTDIVTLPPPASST 196
>SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 683
Score = 25.0 bits (52), Expect = 6.4
Identities = 17/50 (34%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = -2
Query: 478 TVLEIGNLRNAG-HVLSSESLCAEVMVIIVEQVDFASGGQLVTETRDVSV 332
T L+IG A H L+SE +E ++ I + VD+++ L + D SV
Sbjct: 466 TALKIGMALEAHTHALTSEDADSEPVLFITKWVDYSNKYGLGYQLSDESV 515
>SPAC22A12.11 |dak1||dihydroxyacetone kinase
Dak1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 24.6 bits (51), Expect = 8.4
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +2
Query: 284 AGLAYDNVNGHGATLTNTHIPG 349
A A DN+ GA+L + H+PG
Sbjct: 178 AKAAIDNLVSIGASLAHVHVPG 199
>SPAC22F3.12c |rgs1||regulator of G-protein signaling
Rgs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 481
Score = 24.6 bits (51), Expect = 8.4
Identities = 10/41 (24%), Positives = 22/41 (53%)
Frame = +3
Query: 60 TCSLKSLATTSNSMMISRSSGATPGCAGKRVLSLSTLTVPQ 182
+C+L L +T ++ +S+ + C K + S + T+P+
Sbjct: 78 SCTLLQLLSTLENLQLSQVNRIKSRCGSKVLKSTTKFTIPK 118
>SPBC30B4.05 |kap109||karyopherin Kap109|Schizosaccharomyces pombe|chr
2|||Manual
Length = 967
Score = 24.6 bits (51), Expect = 8.4
Identities = 11/38 (28%), Positives = 20/38 (52%)
Frame = +2
Query: 251 LTNQMKLGAATAGLAYDNVNGHGATLTNTHIPGFGDKL 364
+T+ + A LA N+ G + + +TH+PG G +
Sbjct: 922 ITDLKQYCATEMNLANRNMGGRLSQIISTHLPGDGQSV 959
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,091,481
Number of Sequences: 5004
Number of extensions: 40746
Number of successful extensions: 102
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 102
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 198176188
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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