Samia BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I09A02NGRL0001_F23
         (391 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF...   127   3e-30
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id...   126   4e-30
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e...   124   3e-29
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e...   120   5e-28
At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP...    30   0.63 
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim...    30   0.63 
At3g16620.1 68416.m02124 chloroplast outer membrane protein, put...    30   0.63 
At3g46390.1 68416.m05029 hypothetical protein                          29   1.1  
At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containi...    28   1.9  
At1g33010.1 68414.m04065 F-box family protein                          28   1.9  
At3g19430.1 68416.m02464 late embryogenesis abundant protein-rel...    27   4.5  
At2g25660.1 68415.m03075 expressed protein                             27   5.9  

>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
           EF-1-beta, putative nearly identical to eEF-1beta
           [Arabidopsis thaliana] GI:398606
          Length = 231

 Score =  127 bits (306), Expect = 3e-30
 Identities = 59/105 (56%), Positives = 77/105 (73%), Gaps = 3/105 (2%)
 Frame = +2

Query: 65  KAYADKKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGYG 244
           +A + K S K     KSS+++D+KPWDDETDMK++E  VR+I+M+GL WGASKLVPVGYG
Sbjct: 127 RAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWGASKLVPVGYG 186

Query: 245 INKLQIMCVIEDDKVSVDLLTDH---IQDIEDFVQSVDIAAFNTI 370
           I KL IMC I DD VS+D + +    ++ I ++VQS DI AFN I
Sbjct: 187 IKKLHIMCTIVDDLVSIDTMIEEQLTVEPINEYVQSCDIVAFNKI 231


>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
           identical to SP|P48006 Elongation factor 1-beta
           (EF-1-beta) {Arabidopsis thaliana}
          Length = 231

 Score =  126 bits (305), Expect = 4e-30
 Identities = 58/105 (55%), Positives = 78/105 (74%), Gaps = 3/105 (2%)
 Frame = +2

Query: 65  KAYADKKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGYG 244
           +A + K S K     KSS+++D+KPWDDETDMK++E  V++I+M+GL WGASKLVPVGYG
Sbjct: 127 RAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWGASKLVPVGYG 186

Query: 245 INKLQIMCVIEDDKVSVDLLTDH---IQDIEDFVQSVDIAAFNTI 370
           I KLQI+C I DD VS+D + +    ++ I ++VQS DI AFN I
Sbjct: 187 IKKLQILCTIVDDLVSIDTMIEEQLTVEPINEYVQSCDIVAFNKI 231


>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
           (eEF1Balpha1) identical to elongation factor 1B
           alpha-subunit [Arabidopsis thaliana] GI:6686819
          Length = 228

 Score =  124 bits (298), Expect = 3e-29
 Identities = 61/102 (59%), Positives = 76/102 (74%), Gaps = 3/102 (2%)
 Frame = +2

Query: 74  ADKKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGYGINK 253
           A K +KK     KSS++L+VKPWDDETDMK++E  VR+++M GL WGASKLVPVGYGI K
Sbjct: 127 AKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVPVGYGIKK 186

Query: 254 LQIMCVIEDDKVSVD-LLTDHI--QDIEDFVQSVDIAAFNTI 370
           L IM  I DD VSVD L+ DH+  +   +++QSVDI AFN I
Sbjct: 187 LTIMMTIVDDLVSVDNLIEDHLTSEPNNEYIQSVDIVAFNKI 228


>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
           (eEF1Balpha2) identical to elongation factor 1B
           alpha-subunit [Arabidopsis thaliana] GI:6686821
          Length = 224

 Score =  120 bits (288), Expect = 5e-28
 Identities = 60/102 (58%), Positives = 73/102 (71%), Gaps = 3/102 (2%)
 Frame = +2

Query: 74  ADKKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGYGINK 253
           A K +KKP    KSS+++DVKPWDDETDMK++E  VR +EM GL WGASKLVPVGYGI K
Sbjct: 123 AKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPVGYGIKK 182

Query: 254 LQIMCVIEDDKVSVD-LLTDHI--QDIEDFVQSVDIAAFNTI 370
           L IM  I DD VS D L+ D +  +   +++QS DI AFN I
Sbjct: 183 LTIMFTIVDDLVSPDNLIEDFLTSEPNNEYIQSCDIVAFNKI 224


>At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP1)
           identical to biotin carboxyl carrier protein of
           acetyl-CoA carboxylase precursor [Arabidopsis thaliana]
           gi|9759121|dbj|BAB09606
          Length = 280

 Score = 29.9 bits (64), Expect = 0.63
 Identities = 12/42 (28%), Positives = 23/42 (54%)
 Frame = +2

Query: 212 GASKLVPVGYGINKLQIMCVIEDDKVSVDLLTDHIQDIEDFV 337
           G    + VG  + K Q++C++E  K+  ++ +DH   + D V
Sbjct: 221 GEPPFIKVGDKVQKGQVLCIVEAMKLMNEIESDHTGTVVDIV 262


>At5g08415.1 68418.m00991 lipoic acid synthase family protein
           similar to lipoic acid synthase from Arabidopsis
           thaliana [gi:3928758], from Mus musculus [gi:14669826]
           Pfam profile PF04055: radical SAM domain protein
          Length = 394

 Score = 29.9 bits (64), Expect = 0.63
 Identities = 17/44 (38%), Positives = 26/44 (59%)
 Frame = +2

Query: 83  KSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWG 214
           K  KP +I K+SI+L +   D+E  +KE    +R I++D L  G
Sbjct: 287 KISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328


>At3g16620.1 68416.m02124 chloroplast outer membrane protein,
           putative similar to chloroplast protein import component
           Toc159 [Pisum sativum] GI:8489806, chloroplast outer
           envelope protein 86 [Pisum sativum] GI:599958,
           GTP-binding protein [Pisum sativum] GI:576509
          Length = 1089

 Score = 29.9 bits (64), Expect = 0.63
 Identities = 20/71 (28%), Positives = 33/71 (46%), Gaps = 4/71 (5%)
 Frame = +2

Query: 167 MENQVRTIEMDGLLWGASKLVPVGYGINK-LQIMCVIEDDKVSVDLL---TDHIQDIEDF 334
           M  Q+     D L +  + +V    G+ K   I  + ++ K+S D     T  +QDIE F
Sbjct: 441 MAEQLEAAAQDPLDFSCTIMVLGKSGVGKSATINSIFDELKISTDAFQVGTKKVQDIEGF 500

Query: 335 VQSVDIAAFNT 367
           VQ + +   +T
Sbjct: 501 VQGIKVRVIDT 511


>At3g46390.1 68416.m05029 hypothetical protein 
          Length = 69

 Score = 29.1 bits (62), Expect = 1.1
 Identities = 19/69 (27%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
 Frame = +2

Query: 62  LKAYADKKSKKPALIAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWGA--SKLVPV 235
           +K Y+ K  K+  +    +I +D+K      +M + E   + I M G++W    S L+ V
Sbjct: 1   MKRYSTKHFKRILIANNVNIDIDLKTGIVAKNMAKKEKIEQVISMGGIMWEGLNSSLIKV 60

Query: 236 GYGINKLQI 262
              + K QI
Sbjct: 61  DEALLKQQI 69


>At2g34400.1 68415.m04215 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 617

 Score = 28.3 bits (60), Expect = 1.9
 Identities = 14/72 (19%), Positives = 31/72 (43%)
 Frame = +2

Query: 104 IAKSSIILDVKPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGYGINKLQIMCVIEDD 283
           +  S+++ D+K WD+   M+ +      ++  G  W   +   + +      + C  ED 
Sbjct: 535 VISSNVLADMKMWDESAKMRALMRDRGVVKTPGCSWIEIEGELMEFLAGSDYLQCGREDS 594

Query: 284 KVSVDLLTDHIQ 319
               DLL + ++
Sbjct: 595 GSLFDLLVEEMK 606


>At1g33010.1 68414.m04065 F-box family protein
          Length = 361

 Score = 28.3 bits (60), Expect = 1.9
 Identities = 16/50 (32%), Positives = 25/50 (50%)
 Frame = +2

Query: 134 KPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGYGINKLQIMCVIEDD 283
           KP   ET M   ++QVRTI+         + +      NK  +MC+++DD
Sbjct: 317 KPTSTETSMSRKDHQVRTIDQPQQDRCTFESIN-----NKFDVMCLLDDD 361


>At3g19430.1 68416.m02464 late embryogenesis abundant
           protein-related / LEA protein-related similar to late
           embryogenesis abundant protein [Picea glauca] GI:1350543
          Length = 559

 Score = 27.1 bits (57), Expect = 4.5
 Identities = 18/64 (28%), Positives = 34/64 (53%)
 Frame = +2

Query: 188 IEMDGLLWGASKLVPVGYGINKLQIMCVIEDDKVSVDLLTDHIQDIEDFVQSVDIAAFNT 367
           +E++GLL   +++VP+    +++    V EDD ++   L    QD+ D V  V    + +
Sbjct: 431 VEVEGLLKITARVVPITMEDSRIHGYDVKEDDCLAHLDLGFKFQDLSDNVDGVLGQTYRS 490

Query: 368 IYLS 379
            Y+S
Sbjct: 491 NYVS 494


>At2g25660.1 68415.m03075 expressed protein 
          Length = 2146

 Score = 26.6 bits (56), Expect = 5.9
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = -3

Query: 233  QGQALMLPIEGHPFQLCALGFPFLSYLSHHP 141
            QG+A+   +EG    L   GF F S +S +P
Sbjct: 1020 QGEAMPFVVEGLDLDLRMRGFEFFSLVSSYP 1050


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,616,539
Number of Sequences: 28952
Number of extensions: 141291
Number of successful extensions: 464
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 454
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 460
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 557595584
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)