Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_F11
(520 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g48340.1 68418.m05973 expressed protein 33 0.087
At5g24280.1 68418.m02856 expressed protein ; expression supporte... 33 0.087
At1g55350.4 68414.m06326 calpain-type cysteine protease family i... 29 2.5
At1g55350.3 68414.m06325 calpain-type cysteine protease family i... 29 2.5
At1g55350.2 68414.m06324 calpain-type cysteine protease family i... 29 2.5
At1g55350.1 68414.m06323 calpain-type cysteine protease family i... 29 2.5
At1g77520.1 68414.m09027 O-methyltransferase family 2 protein si... 27 5.7
At1g54070.1 68414.m06161 dormancy/auxin associated protein-related 27 5.7
At5g28810.1 68418.m03542 hypothetical protein 27 7.6
At3g27970.1 68416.m03491 exonuclease family protein contains exo... 27 7.6
At1g50980.1 68414.m05731 F-box family protein contains F-box dom... 27 7.6
>At5g48340.1 68418.m05973 expressed protein
Length = 510
Score = 33.5 bits (73), Expect = 0.087
Identities = 18/50 (36%), Positives = 28/50 (56%)
Frame = +2
Query: 365 LLTSTEINTSRPFYKLTKEVNMNDTVKIRKRLK*QLCVSIKNCKSF*VIL 514
LL S+ IN R +L ++ N ND ++ K ++C ++N SF VIL
Sbjct: 308 LLLSSIINQGRQLLRLVRDENGNDEGELLKETMAEICRGLENESSFSVIL 357
>At5g24280.1 68418.m02856 expressed protein ; expression supported by
MPSS
Length = 1634
Score = 33.5 bits (73), Expect = 0.087
Identities = 21/67 (31%), Positives = 35/67 (52%)
Frame = +1
Query: 58 TLPEDEGEYTCEVDNGVGKPQKHSLKLTVVSAPKYEQKPEKVIVVKHGQDVTIPCKVTGL 237
+LPE+EG ++C V + + S+K+ V SAP E++ E H + T P +G+
Sbjct: 1199 SLPENEGVFSCRVFHSRYPELQMSIKIQVTSAPTSERE-ESGYSTPHSK--TTPPPESGI 1255
Query: 238 PAPKVVW 258
P+ W
Sbjct: 1256 PSITNPW 1262
>At1g55350.4 68414.m06326 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 28.7 bits (61), Expect = 2.5
Identities = 11/30 (36%), Positives = 19/30 (63%)
Frame = -3
Query: 287 PPLRGFALWLQTTLGAGRPVTLHGIVTSCP 198
P ++GF LW+ L AG ++L I+++ P
Sbjct: 757 PRIKGFILWICVVLFAGSVISLGAIISAKP 786
>At1g55350.3 68414.m06325 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 28.7 bits (61), Expect = 2.5
Identities = 11/30 (36%), Positives = 19/30 (63%)
Frame = -3
Query: 287 PPLRGFALWLQTTLGAGRPVTLHGIVTSCP 198
P ++GF LW+ L AG ++L I+++ P
Sbjct: 757 PRIKGFILWICVVLFAGSVISLGAIISAKP 786
>At1g55350.2 68414.m06324 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 28.7 bits (61), Expect = 2.5
Identities = 11/30 (36%), Positives = 19/30 (63%)
Frame = -3
Query: 287 PPLRGFALWLQTTLGAGRPVTLHGIVTSCP 198
P ++GF LW+ L AG ++L I+++ P
Sbjct: 757 PRIKGFILWICVVLFAGSVISLGAIISAKP 786
>At1g55350.1 68414.m06323 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 28.7 bits (61), Expect = 2.5
Identities = 11/30 (36%), Positives = 19/30 (63%)
Frame = -3
Query: 287 PPLRGFALWLQTTLGAGRPVTLHGIVTSCP 198
P ++GF LW+ L AG ++L I+++ P
Sbjct: 757 PRIKGFILWICVVLFAGSVISLGAIISAKP 786
>At1g77520.1 68414.m09027 O-methyltransferase family 2 protein
similar to caffeic acid 3-O-methyltransferase GB:O23760
[Clarkia breweri], [SP|Q00763] [Populus tremuloides]
Length = 381
Score = 27.5 bits (58), Expect = 5.7
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -3
Query: 389 YLSPCSLVALQP*YPVSPFCTPLMTRPLSLTVA 291
+LSPC + P P +P L+ R LSL V+
Sbjct: 66 WLSPCEIACSLPTKPTNPEAPVLLDRMLSLLVS 98
>At1g54070.1 68414.m06161 dormancy/auxin associated protein-related
Length = 123
Score = 27.5 bits (58), Expect = 5.7
Identities = 22/68 (32%), Positives = 33/68 (48%), Gaps = 4/68 (5%)
Frame = +1
Query: 97 DNGVGKPQKHSLKLTVVSAP---KYEQKPEKVIVVKHGQDVTIPCKVTGLP-APKVVWSH 264
DNG+GK +KH TV S+P +Q ++V K +V K+ P +P S+
Sbjct: 18 DNGLGKLRKHDSLSTVRSSPPSLSSDQVTRSIMVTKGNNNVRGLRKLKMDPDSPTCSSSN 77
Query: 265 NAKPLSGG 288
PL+ G
Sbjct: 78 PGTPLTPG 85
>At5g28810.1 68418.m03542 hypothetical protein
Length = 560
Score = 27.1 bits (57), Expect = 7.6
Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +1
Query: 22 NRTSGKRLLFKTTLPEDEGEYTCEVDNGVGKPQKHSLKLTVVSAPKYE-QKPEK 180
N+ +G ++L K + + + + VGKP K +K TVV K + KPEK
Sbjct: 440 NKEAGTKVLTKKKVAKSDKKKIIVNVEKVGKP-KPEMKKTVVKVEKVDSSKPEK 492
>At3g27970.1 68416.m03491 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 354
Score = 27.1 bits (57), Expect = 7.6
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -1
Query: 118 GVCRLHCRLRKCIRPRLLGVLS*RE 44
GVC+ HCR + +R L+G L +E
Sbjct: 47 GVCKKHCRSFESLREHLIGPLPKQE 71
>At1g50980.1 68414.m05731 F-box family protein contains F-box domain
Pfam:PF00646
Length = 370
Score = 27.1 bits (57), Expect = 7.6
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = -3
Query: 320 MTRPLSLTVALPPLRGFALWLQTTLGAGRPVTLHGIVTSCP 198
+T + T+ +P LR L + T G RP +HG V + P
Sbjct: 164 LTNVMIFTIDVPTLR--ILSIDNTSGKSRPKGVHGFVINTP 202
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,111,830
Number of Sequences: 28952
Number of extensions: 225582
Number of successful extensions: 624
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 611
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 624
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 947539968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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