Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_E09
(594 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 155 3e-38
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 154 5e-38
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 151 3e-37
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 150 8e-37
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 91 7e-19
At4g39060.1 68417.m05532 F-box family protein low similarity to ... 30 1.0
At4g01810.1 68417.m00238 protein transport protein-related relat... 29 3.1
At2g37660.1 68415.m04619 expressed protein 28 4.1
At1g66950.1 68414.m07612 ABC transporter family protein similar ... 28 4.1
At5g09450.1 68418.m01094 pentatricopeptide (PPR) repeat-containi... 28 5.4
At4g19330.1 68417.m02848 kelch repeat-containing F-box family pr... 27 9.4
At3g17380.1 68416.m02221 meprin and TRAF homology domain-contain... 27 9.4
At1g68130.1 68414.m07782 zinc finger (C2H2 type) family protein ... 27 9.4
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 155 bits (375), Expect = 3e-38
Identities = 67/122 (54%), Positives = 88/122 (72%)
Frame = +3
Query: 198 AFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWIG 377
A AG+++G+EIWRI+NF+PV V + GKF+ GDSYI+LKTT+ + +L DIHYW+G
Sbjct: 11 ALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLG 70
Query: 378 SETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGFN 557
++SQDEAGA A++TV LD GG AVQ+RE GHE+ FL YF + +GG SGFN
Sbjct: 71 KDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGFN 130
Query: 558 HV 563
HV
Sbjct: 131 HV 132
Score = 41.1 bits (92), Expect = 5e-04
Identities = 32/153 (20%), Positives = 65/153 (42%), Gaps = 2/153 (1%)
Frame = +3
Query: 120 IRQQSVNTQITSLSDKDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYK 299
+++Q VN Q + + +K P G +++WRI E + +++ + KFY
Sbjct: 364 LQRQGVNVQ-GLVKTSSSSSKDEPKPYIDGTGN---LQVWRINCEEKILLEAAEQSKFYS 419
Query: 300 GDSYIILKT--TSDKRNNLSWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREA 473
GD YI+ + D+ +L + W G ++ +++ +A L + + Q R
Sbjct: 420 GDCYILQYSYPGEDREEHL---VGTWFGKQSVEEDRASAISLANKMVESMKFVPAQARIN 476
Query: 474 MGHESNHFLQYFTSALQYLDGGNPSGFNHVVTN 572
G E F S + + G + + ++ N
Sbjct: 477 EGKEPIQFFVIMQSFITFKGGVSDAFKKYIAEN 509
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 154 bits (373), Expect = 5e-38
Identities = 70/124 (56%), Positives = 85/124 (68%)
Frame = +3
Query: 195 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 374
PAF AG++AG+EIWRI+NF P + GKF+ GDSYI+LKTT+ K L DIHYW+
Sbjct: 10 PAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHDIHYWL 69
Query: 375 GSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGF 554
G +TSQDEAG AA+ TV LD GG AVQ+RE GHE+ FL YF + +GG SGF
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGF 129
Query: 555 NHVV 566
HVV
Sbjct: 130 KHVV 133
Score = 43.2 bits (97), Expect = 1e-04
Identities = 26/113 (23%), Positives = 47/113 (41%)
Frame = +3
Query: 228 VEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWIGSETSQDEAGA 407
+++WR+ +Q+ D KFY GD Y+ + + I W G ++ ++E G+
Sbjct: 394 LQVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKE-EVLIGTWFGKQSVEEERGS 452
Query: 408 AAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGFNHVV 566
A + + + Q R G E F S + + GG SG+ +
Sbjct: 453 AVSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVF-KGGISSGYKKYI 504
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 151 bits (366), Expect = 3e-37
Identities = 70/123 (56%), Positives = 85/123 (69%)
Frame = +3
Query: 195 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 374
PAF AG++ G EIWRI+NFE V V + GKFY GD+YI+L+TT +K +DIH+WI
Sbjct: 8 PAFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 67
Query: 375 GSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGF 554
G +TSQDEAG AA+ TV LD GG AVQHRE GHES+ FL YF + L+GG SGF
Sbjct: 68 GKDTSQDEAGTAAVKTVELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGF 127
Query: 555 NHV 563
V
Sbjct: 128 KTV 130
Score = 51.6 bits (118), Expect = 4e-07
Identities = 32/130 (24%), Positives = 53/130 (40%)
Frame = +3
Query: 165 KDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRN 344
K A + P + G+ +E+W + + +D GK Y GD Y++L T
Sbjct: 374 KSAPVNEDIPPLLESGGK---LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGER 430
Query: 345 NLSWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQ 524
+ + W G ++ ++ A L + + G VQ R G E F+ F +
Sbjct: 431 KDEYFLSCWFGKKSIPEDQDTAIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALF-QPMV 489
Query: 525 YLDGGNPSGF 554
L GG SG+
Sbjct: 490 VLKGGLSSGY 499
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 150 bits (363), Expect = 8e-37
Identities = 68/120 (56%), Positives = 84/120 (70%)
Frame = +3
Query: 195 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 374
PAF G++ G EIWRI+NFEPV V + GKFY GD+YI+L+TT +K +DIH+WI
Sbjct: 10 PAFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 69
Query: 375 GSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDGGNPSGF 554
G +TSQDEAG AA+ TV LD GG AVQ+RE GHES+ FL YF + L+GG SGF
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGGVASGF 129
Score = 54.8 bits (126), Expect = 4e-08
Identities = 39/154 (25%), Positives = 63/154 (40%)
Frame = +3
Query: 120 IRQQSVNTQITSLSDKDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYK 299
++QQ V + LS K + P G+ +E+W I + GK Y
Sbjct: 364 LKQQGVG--LKGLS-KSTPVNEDIPPLLEGGGK---LEVWYIDANSKTVLSKDHVGKLYS 417
Query: 300 GDSYIILKTTSDKRNNLSWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMG 479
GD Y++L T + + W G ++Q++ A L + + G VQ R G
Sbjct: 418 GDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQETAVRLASTMTNSLKGRPVQARIFEG 477
Query: 480 HESNHFLQYFTSALQYLDGGNPSGFNHVVTNPGA 581
E F+ F + L GG SG+ + +T G+
Sbjct: 478 KEPPQFVALF-QHMVVLKGGLSSGYKNSMTEKGS 510
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 90.6 bits (215), Expect = 7e-19
Identities = 39/122 (31%), Positives = 70/122 (57%)
Frame = +3
Query: 171 ARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNL 350
+R + AF G ++G+EIW + N + +++ FGKF+ G++Y++L+T K +
Sbjct: 2 SRLSKDIDSAFQGVGTKSGLEIWCVYNKQLISIPKSSFGKFHSGNAYLVLRTFLRKIESP 61
Query: 351 SWDIHYWIGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYL 530
+DIHYW+G + ++ ++ A+ + LD G VQ+RE G E+ FL YF + +
Sbjct: 62 QYDIHYWLGIDANEVDSILASDKALDLDAALGCCTVQYREVQGQETEKFLSYFKPCIIPV 121
Query: 531 DG 536
+G
Sbjct: 122 EG 123
Score = 35.9 bits (79), Expect = 0.020
Identities = 26/115 (22%), Positives = 48/115 (41%), Gaps = 1/115 (0%)
Frame = +3
Query: 195 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYII-LKTTSDKRNNLSWDIHYW 371
P + N + +++WR+ + + D K + GD Y++ K T +R ++ W
Sbjct: 382 PLYTNC--RDNLKVWRVDGDDVSLLSIPDQTKLFTGDCYLVQYKYTYKERTEHL--LYVW 437
Query: 372 IGSETSQDEAGAAAILTVGLDDKFGGAAVQHREAMGHESNHFLQYFTSALQYLDG 536
IG E+ Q + A + G +V G+E + F F S + + G
Sbjct: 438 IGCESIQQDRADAITNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQSLVVFKGG 492
>At4g39060.1 68417.m05532 F-box family protein low similarity to
SKP1 interacting partner 6 [Arabidopsis thaliana]
GI:10716957; contains Pfam profile PF00646: F-box domain
Length = 375
Score = 30.3 bits (65), Expect = 1.0
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = -3
Query: 577 PGFVTTWLKPDGLPPSKYCSADVKYCRKWLLSWPIASRCCTAAPPNL 437
P + + W+KPD ++YC+ K R L+ P+ S T PPNL
Sbjct: 64 PIWFSLWIKPDDQTLNQYCNIQEKSTRNLLV--PVPS-SYTPFPPNL 107
>At4g01810.1 68417.m00238 protein transport protein-related related
to Sec23 protein [Homo sapiens] gi|1296664|emb|CAA65774
Length = 880
Score = 28.7 bits (61), Expect = 3.1
Identities = 19/56 (33%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
Frame = +3
Query: 258 PVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSW--DIHYWIGSETSQDEAGAAAIL 419
P + ++ G F + +Y L SDK L D+ W+G+E S DE +AA+L
Sbjct: 733 PRCLMHQEGGTFEELPAYD-LSMQSDKAVILDHGTDVFIWLGAELSADEVKSAAVL 787
>At2g37660.1 68415.m04619 expressed protein
Length = 325
Score = 28.3 bits (60), Expect = 4.1
Identities = 20/61 (32%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = +3
Query: 369 WIGSETSQDEAGAAAILTVGLDDKFGGAAVQH-REAMGHESNHFLQYFTSALQYL-DGGN 542
WIG + D A AA + + L GG + H ++G+ + L + A QYL D G
Sbjct: 182 WIGQKNQIDAAKAAGVKQIVLVGSMGGTNINHPLNSIGNA--NILVWKRKAEQYLADSGI 239
Query: 543 P 545
P
Sbjct: 240 P 240
>At1g66950.1 68414.m07612 ABC transporter family protein similar to
PDR5-like ABC transporter GI:1514643 from [Spirodela
polyrhiza]
Length = 1454
Score = 28.3 bits (60), Expect = 4.1
Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 3/52 (5%)
Frame = +3
Query: 447 GAAVQHREAMGHESNHFLQYFTS---ALQYLDGGNPSGFNHVVTNPGAEKRM 593
G V + ++GH S ++YF + + DG NP+ + VT P E +M
Sbjct: 1081 GGQVIYAGSLGHHSQKLVEYFEAVEGVPKINDGYNPATWMLDVTTPSMESQM 1132
>At5g09450.1 68418.m01094 pentatricopeptide (PPR) repeat-containing
protein low similiarity to DNA-binding protein [Triticum
aestivum] GI:6958202; contains Pfam profile: PF01535 PPR
repeat
Length = 409
Score = 27.9 bits (59), Expect = 5.4
Identities = 10/33 (30%), Positives = 22/33 (66%)
Frame = -1
Query: 330 MSSLELYRNLLCRTSQSLSIGLRLVQNSESAKF 232
M++ L+ +L CR + + +G ++N+ES++F
Sbjct: 1 MATRSLFHSLRCRLTNNGVLGSNFIRNAESSRF 33
>At4g19330.1 68417.m02848 kelch repeat-containing F-box family
protein very low similarity to SKP1 interacting partner
6 [Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 537
Score = 27.1 bits (57), Expect = 9.4
Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 1/34 (2%)
Frame = -3
Query: 559 WLKPDGLPPSKYCSADVKYCRKWLLSWPIA-SRC 461
W+KP P + + D+K WLL P SRC
Sbjct: 248 WIKPYDHQPLTHWTIDIKCTGHWLLPMPSPYSRC 281
>At3g17380.1 68416.m02221 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 309
Score = 27.1 bits (57), Expect = 9.4
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = +3
Query: 234 IWRIQNFEPVAVQSKDFGKFYKGD 305
+W+I+NF + +S D F+ GD
Sbjct: 177 VWKIENFSKLDKESYDSNAFFAGD 200
>At1g68130.1 68414.m07782 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 419
Score = 27.1 bits (57), Expect = 9.4
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = -2
Query: 149 DLSINRLLPYGSARVRTRPKGHQQPHTYEFHFV 51
DLSI +LP + R P QQP T + FV
Sbjct: 232 DLSIGPILPGHPLQRRQSPPSEQQPSTLLYPFV 264
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,120,180
Number of Sequences: 28952
Number of extensions: 301566
Number of successful extensions: 843
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 821
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 842
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1180950720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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