Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_D16
(599 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast... 168 3e-42
At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast... 168 3e-42
At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondri... 166 9e-42
At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic... 166 1e-41
At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast... 163 6e-41
At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic... 144 5e-35
At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic... 141 3e-34
At3g54460.1 68416.m06025 SNF2 domain-containing protein / helica... 31 0.77
At4g20320.1 68417.m02967 CTP synthase, putative / UTP--ammonia l... 29 3.1
At4g18240.1 68417.m02709 starch synthase-related protein contain... 29 3.1
At5g51290.1 68418.m06358 ceramide kinase-related contains weak s... 28 4.1
At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containi... 28 5.4
At1g72110.1 68414.m08335 expressed protein 28 5.4
At1g75530.1 68414.m08778 forkhead-associated domain-containing p... 27 7.2
At3g49730.1 68416.m05437 pentatricopeptide (PPR) repeat-containi... 27 9.5
At1g23400.1 68414.m02930 expressed protein 27 9.5
>At4g31990.2 68417.m04554 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 168 bits (408), Expect = 3e-42
Identities = 80/166 (48%), Positives = 111/166 (66%)
Frame = +3
Query: 99 WSNVPMGPPDVILGITEAYKKDTHPNKVNLGVGAYRDDEGKPFVLPSVRKAEEILHKKGL 278
+ + M PPD ILG++EA+K DT+ K+NLGVGAYR +E +P+VL V+KAE ++ ++G
Sbjct: 53 FEGITMAPPDPILGVSEAFKADTNGMKLNLGVGAYRTEELQPYVLNVVKKAENLMLERGD 112
Query: 279 NHEYAPISGEAAYTDAVAKLAFGEDSQVIQNKSNCTVQTLSGTGALRLGLEFITNHYAKA 458
N EY PI G AA+ A A+L FG VI+ + T+Q LSGTG+LRL I ++ A
Sbjct: 113 NKEYLPIEGLAAFNKATAELLFGAGHPVIKEQRVATIQGLSGTGSLRLAAALIERYFPGA 172
Query: 459 KEIWMPTPTWGNHPQICNMLKLPHKKYRYFDPKTNGFDLQGALEDI 596
K + + +PTWGNH I N K+P +YRY+DPKT G D +G + DI
Sbjct: 173 KVV-ISSPTWGNHKNIFNDAKVPWSEYRYYDPKTIGLDFEGMIADI 217
>At4g31990.1 68417.m04553 aspartate aminotransferase, chloroplast /
transaminase A (ASP5) (AAT1) nearly identical to
SP|P46248 Aspartate aminotransferase, chloroplast
precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis
thaliana}
Length = 453
Score = 168 bits (408), Expect = 3e-42
Identities = 80/166 (48%), Positives = 111/166 (66%)
Frame = +3
Query: 99 WSNVPMGPPDVILGITEAYKKDTHPNKVNLGVGAYRDDEGKPFVLPSVRKAEEILHKKGL 278
+ + M PPD ILG++EA+K DT+ K+NLGVGAYR +E +P+VL V+KAE ++ ++G
Sbjct: 53 FEGITMAPPDPILGVSEAFKADTNGMKLNLGVGAYRTEELQPYVLNVVKKAENLMLERGD 112
Query: 279 NHEYAPISGEAAYTDAVAKLAFGEDSQVIQNKSNCTVQTLSGTGALRLGLEFITNHYAKA 458
N EY PI G AA+ A A+L FG VI+ + T+Q LSGTG+LRL I ++ A
Sbjct: 113 NKEYLPIEGLAAFNKATAELLFGAGHPVIKEQRVATIQGLSGTGSLRLAAALIERYFPGA 172
Query: 459 KEIWMPTPTWGNHPQICNMLKLPHKKYRYFDPKTNGFDLQGALEDI 596
K + + +PTWGNH I N K+P +YRY+DPKT G D +G + DI
Sbjct: 173 KVV-ISSPTWGNHKNIFNDAKVPWSEYRYYDPKTIGLDFEGMIADI 217
>At2g30970.1 68415.m03777 aspartate aminotransferase, mitochondrial
/ transaminase A (ASP1) identical to SP|P46643 Aspartate
aminotransferase, mitochondrial precursor (EC 2.6.1.1)
(Transaminase A) {Arabidopsis thaliana}
Length = 430
Score = 166 bits (404), Expect = 9e-42
Identities = 79/173 (45%), Positives = 107/173 (61%)
Frame = +3
Query: 78 LRASSTWWSNVPMGPPDVILGITEAYKKDTHPNKVNLGVGAYRDDEGKPFVLPSVRKAEE 257
LR+ S+WW +V P D ILG+TEA+ D P KVN+GVGAYRDD GKP VL VR+AE+
Sbjct: 26 LRSMSSWWKSVEPAPKDPILGVTEAFLADPSPEKVNVGVGAYRDDNGKPVVLECVREAEK 85
Query: 258 ILHKKGLNHEYAPISGEAAYTDAVAKLAFGEDSQVIQNKSNCTVQTLSGTGALRLGLEFI 437
L EY P+ G A D KLA+G++S+ I++K VQTLSGTGA RL +F
Sbjct: 86 RLAGSTF-MEYLPMGGSAKMVDLTLKLAYGDNSEFIKDKRIAAVQTLSGTGACRLFADF- 143
Query: 438 TNHYAKAKEIWMPTPTWGNHPQICNMLKLPHKKYRYFDPKTNGFDLQGALEDI 596
++ +I++P PTW NH I ++P K Y Y+ P+T G D ++D+
Sbjct: 144 QKRFSPGSQIYIPVPTWSNHHNIWKDAQVPQKTYHYYHPETKGLDFSALMDDV 196
>At5g19550.1 68418.m02328 aspartate aminotransferase, cytoplasmic
isozyme 1 / transaminase A (ASP2) identical to SP|P46645
Aspartate aminotransferase, cytoplasmic isozyme 1 (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana}
Length = 405
Score = 166 bits (403), Expect = 1e-41
Identities = 82/167 (49%), Positives = 107/167 (64%), Gaps = 1/167 (0%)
Frame = +3
Query: 99 WSNVPMGPPDVILGITEAYKKDTHPNKVNLGVGAYRDDEGKPFVLPSVRKAE-EILHKKG 275
+SNV P D ILG+T AY D P K+NLGVGAYR +EGKP VL VRKAE ++++
Sbjct: 5 FSNVARAPEDPILGVTVAYNNDPSPVKINLGVGAYRTEEGKPLVLDVVRKAEQQLVNDPS 64
Query: 276 LNHEYAPISGEAAYTDAVAKLAFGEDSQVIQNKSNCTVQTLSGTGALRLGLEFITNHYAK 455
EY PI G + + AKL G DS I TVQ LSGTG+LR+G EF+ HY +
Sbjct: 65 RVKEYIPIVGISDFNKLSAKLILGADSPAITESRVTTVQCLSGTGSLRVGAEFLKTHYHQ 124
Query: 456 AKEIWMPTPTWGNHPQICNMLKLPHKKYRYFDPKTNGFDLQGALEDI 596
+ I++P PTWGNHP++ N+ L + +RY+DP T G D +G LED+
Sbjct: 125 S-VIYIPKPTWGNHPKVFNLAGLSVEYFRYYDPATRGLDFKGLLEDL 170
>At5g11520.1 68418.m01344 aspartate aminotransferase, chloroplast /
transaminase A (ASP3) (YLS4) identical to SP|P46644
Aspartate aminotransferase, chloroplast precursor (EC
2.6.1.1) (Transaminase A) {Arabidopsis thaliana};
identical to cDNA YLS4 mRNA for aspartate
aminotransferase (ASP3), partial cds GI:13122285
Length = 449
Score = 163 bits (397), Expect = 6e-41
Identities = 86/196 (43%), Positives = 117/196 (59%), Gaps = 1/196 (0%)
Frame = +3
Query: 12 LKKLTTQVLKNNNIDYFVGCTALRASSTWWSNVPMGPPDVILGITEAYKKDTHPNKVNLG 191
L+ L + +N + T+ + +S++ P D ILG+T AY KD P K+NLG
Sbjct: 20 LRHLNSGSDSDNLSSLYASPTSGGTGGSVFSHLVQAPEDPILGVTVAYNKDPSPVKLNLG 79
Query: 192 VGAYRDDEGKPFVLPSVRKAE-EILHKKGLNHEYAPISGEAAYTDAVAKLAFGEDSQVIQ 368
VGAYR +EGKP VL VRKAE ++++ + EY PI G + AKL G DS I+
Sbjct: 80 VGAYRTEEGKPLVLNVVRKAEQQLINDRTRIKEYLPIVGLVEFNKLSAKLILGADSPAIR 139
Query: 369 NKSNCTVQTLSGTGALRLGLEFITNHYAKAKEIWMPTPTWGNHPQICNMLKLPHKKYRYF 548
TV+ LSGTG+LR+G EF+ HY + K I++ PTWGNHP+I + L K YRY+
Sbjct: 140 ENRITTVECLSGTGSLRVGGEFLAKHYHQ-KTIYITQPTWGNHPKIFTLAGLTVKTYRYY 198
Query: 549 DPKTNGFDLQGALEDI 596
DP T G + QG LED+
Sbjct: 199 DPATRGLNFQGLLEDL 214
>At1g62800.1 68414.m07089 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 403
Score = 144 bits (348), Expect = 5e-35
Identities = 71/165 (43%), Positives = 99/165 (60%)
Frame = +3
Query: 102 SNVPMGPPDVILGITEAYKKDTHPNKVNLGVGAYRDDEGKPFVLPSVRKAEEILHKKGLN 281
S+V P D +L + A + D P K+NL G YR +EGKP VL VR+AE+ L L+
Sbjct: 6 SSVLPAPEDPVLSVIFACRDDPSPVKLNLSAGTYRTEEGKPLVLDVVRRAEQQL-ANDLD 64
Query: 282 HEYAPISGEAAYTDAVAKLAFGEDSQVIQNKSNCTVQTLSGTGALRLGLEFITNHYAKAK 461
EY P++G + KL G+DS ++ T Q LSGTG+LR+G EF+ H K
Sbjct: 65 KEYLPLNGLPEFNKLSTKLILGDDSPALKENRVVTTQCLSGTGSLRVGAEFLATHN-KES 123
Query: 462 EIWMPTPTWGNHPQICNMLKLPHKKYRYFDPKTNGFDLQGALEDI 596
I++P PTWGNHP+I + L + +RY+DPK+ G D +G LED+
Sbjct: 124 VIFVPNPTWGNHPRIFTLAGLSVQYFRYYDPKSRGLDFKGMLEDL 168
>At1g62800.2 68414.m07090 aspartate aminotransferase, cytoplasmic
isozyme 2 / transaminase A (ASP4) identical to aspartate
aminotransferase, cytoplasmic isozyme 2 SP:P46646
[Arabidopsis thaliana]
Length = 405
Score = 141 bits (342), Expect = 3e-34
Identities = 70/166 (42%), Positives = 99/166 (59%), Gaps = 1/166 (0%)
Frame = +3
Query: 102 SNVPMGPPDVILGITEAYKKDTHPNKVNLGVGAYRDDEGKPFVLPSVRKAEEIL-HKKGL 278
S+V P D +L + A + D P K+NL G YR +EGKP VL VR+AE+ L +
Sbjct: 6 SSVLPAPEDPVLSVIFACRDDPSPVKLNLSAGTYRTEEGKPLVLDVVRRAEQQLANDLSR 65
Query: 279 NHEYAPISGEAAYTDAVAKLAFGEDSQVIQNKSNCTVQTLSGTGALRLGLEFITNHYAKA 458
+ EY P++G + KL G+DS ++ T Q LSGTG+LR+G EF+ H K
Sbjct: 66 DKEYLPLNGLPEFNKLSTKLILGDDSPALKENRVVTTQCLSGTGSLRVGAEFLATHN-KE 124
Query: 459 KEIWMPTPTWGNHPQICNMLKLPHKKYRYFDPKTNGFDLQGALEDI 596
I++P PTWGNHP+I + L + +RY+DPK+ G D +G LED+
Sbjct: 125 SVIFVPNPTWGNHPRIFTLAGLSVQYFRYYDPKSRGLDFKGMLEDL 170
>At3g54460.1 68416.m06025 SNF2 domain-containing protein / helicase
domain-containing protein / F-box family protein similar
to SP|P36607 DNA repair protein rad8 {Schizosaccharomyces
pombe}; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain, PF00646: F-box domain
Length = 1378
Score = 30.7 bits (66), Expect = 0.77
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +3
Query: 360 VIQNKSNCTVQTLSGTGALRLGLEFITNHYAKAKEIW 470
+ QN ++C + G+GAL L L F+T H + IW
Sbjct: 1238 MFQNDADCMALLMDGSGALGLDLSFVT-HVFLMEPIW 1273
>At4g20320.1 68417.m02967 CTP synthase, putative / UTP--ammonia
ligase, putative similar to SP|P17812 CTP synthase (EC
6.3.4.2) (UTP--ammonia ligase) {Homo sapiens}; contains
Pfam profile PF00117: glutamine amidotransferase class-I
Length = 563
Score = 28.7 bits (61), Expect = 3.1
Identities = 20/76 (26%), Positives = 39/76 (51%), Gaps = 6/76 (7%)
Frame = +1
Query: 148 KLTRKTHIPTRSTLVLEHTEMMKGNHLSYHQ------*GRQKRFFIKKV*IMNMLRSVVK 309
K+T K H+P R +V ++TE++ ++LS H+ R+K+ I + ++ + K
Sbjct: 289 KMTDKLHVPVRIAVVGKYTELL-DSYLSIHKALLHASVARRKKLIIDWISASDLEQGAKK 347
Query: 310 QHILMRSPNWPLVKTA 357
++ W L+K A
Sbjct: 348 ENPDAYKAAWKLLKGA 363
>At4g18240.1 68417.m02709 starch synthase-related protein contains
similarity to starch synthase GI:4582783 from [Vigna
unguiculata]
Length = 1040
Score = 28.7 bits (61), Expect = 3.1
Identities = 22/84 (26%), Positives = 38/84 (45%), Gaps = 1/84 (1%)
Frame = +3
Query: 225 FVLPSVRKAEEILHKKGLNHEYAPISGEAA-YTDAVAKLAFGEDSQVIQNKSNCTVQTLS 401
F++PS+ + + + + PI+ + D+V + +D+ Q ++ T QT
Sbjct: 922 FIIPSIFEPCGLTQMIAMRYGSIPIARKTGGLNDSVFDI--DDDTIPTQFQNGFTFQTAD 979
Query: 402 GTGALRLGLEFITNHYAKAKEIWM 473
G LE NHY K +E WM
Sbjct: 980 EQG-FNYALERAFNHYKKDEEKWM 1002
>At5g51290.1 68418.m06358 ceramide kinase-related contains weak
similarity to ceramide kinases (GI:21624342) [Mus
musculus]
Length = 608
Score = 28.3 bits (60), Expect = 4.1
Identities = 12/31 (38%), Positives = 15/31 (48%)
Frame = +1
Query: 307 KQHILMRSPNWPLVKTARSFKIRATVLCRLC 399
K + RS WP T RS KI C++C
Sbjct: 437 KASLHTRSKTWPFRNTTRSEKILCRANCKIC 467
>At5g13230.1 68418.m01520 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 822
Score = 27.9 bits (59), Expect = 5.4
Identities = 11/37 (29%), Positives = 21/37 (56%)
Frame = +1
Query: 127 M*SWVSLKLTRKTHIPTRSTLVLEHTEMMKGNHLSYH 237
M W+++K TR ++P R+ ++L+ + K L H
Sbjct: 717 MLEWLNMKATRAGYVPDRNAVLLDMDDEEKDKRLWVH 753
>At1g72110.1 68414.m08335 expressed protein
Length = 479
Score = 27.9 bits (59), Expect = 5.4
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -1
Query: 224 WFPFIISVCSNTKVDLVGMCVFLVSFSDTQDHI 126
W FI+ + +T V+++ VF+ SDT HI
Sbjct: 190 WLWFIVRLMFHTCVEVIKSIVFICRASDTSAHI 222
>At1g75530.1 68414.m08778 forkhead-associated domain-containing
protein / FHA domain-containing protein
Length = 555
Score = 27.5 bits (58), Expect = 7.2
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +3
Query: 462 EIWMPTPTWGNHPQICN 512
EIW+P P + HP+I N
Sbjct: 231 EIWIPPPPYDPHPEIVN 247
>At3g49730.1 68416.m05437 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 1184
Score = 27.1 bits (57), Expect = 9.5
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = -1
Query: 524 QLEHVTDLRVVTPRRGRHPDLLGFRIVI 441
Q E +L RRG HPDLL + +VI
Sbjct: 392 QFEECLELIEKMKRRGCHPDLLIYNVVI 419
>At1g23400.1 68414.m02930 expressed protein
Length = 564
Score = 27.1 bits (57), Expect = 9.5
Identities = 13/30 (43%), Positives = 15/30 (50%)
Frame = +3
Query: 477 TPTWGNHPQICNMLKLPHKKYRYFDPKTNG 566
T T PQ LKLPH + RY+ P G
Sbjct: 56 TDTNPKKPQSNPALKLPHHRTRYYKPVKEG 85
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,885,202
Number of Sequences: 28952
Number of extensions: 317403
Number of successful extensions: 782
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 758
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 770
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1187288784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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