BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I09A02NGRL0001_C21 (455 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 28 3.4 At5g15540.1 68418.m01819 expressed protein low similarity to DNA... 27 4.5 At2g43270.1 68415.m05378 F-box family protein contains Pfam PF00... 27 4.5 At5g40480.1 68418.m04909 expressed protein ; expression supporte... 27 6.0 At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein si... 27 6.0 At1g56590.1 68414.m06508 clathrin adaptor complexes medium subun... 27 6.0 At3g21550.1 68416.m02718 expressed protein contains Pfam profile... 27 7.9 >At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly identical to ubiquitin-protein ligase 2 [Arabidopsis thaliana] GI:7108523; E3, HECT-domain protein family; similar to ubiquitin-protein ligase 2 GI:7108523 from [Arabidopsis thaliana] Length = 3658 Score = 27.9 bits (59), Expect = 3.4 Identities = 14/35 (40%), Positives = 23/35 (65%) Frame = +1 Query: 70 LNETIAQYPTNKDVARTFDIMQTDLQCCGINGPED 174 L++TI + ++V+RT D ++ + C G NGPED Sbjct: 442 LDDTI--FRLKQEVSRTEDDVKEIVCCSGSNGPED 474 >At5g15540.1 68418.m01819 expressed protein low similarity to DNA repair and meiosis protein Rad9 [Coprinus cinereus] GI:1353390, SP|Q09725 Sister chromatid cohesion protein mis4 {Schizosaccharomyces pombe} Length = 1755 Score = 27.5 bits (58), Expect = 4.5 Identities = 25/94 (26%), Positives = 37/94 (39%), Gaps = 2/94 (2%) Frame = +1 Query: 40 KDLETSIVKHLNETIAQYPTNKDVARTFDIMQTDLQCCGINGPEDWAAHNLTIPNTC--C 213 +DL+ IV+H T+ +K + I++ LQ E + N I C Sbjct: 1096 QDLKHMIVRHSFLTVVHACVSKLAGKGVSIVEHLLQFF-FKRLEAQGSDNTQIAGRSLFC 1154 Query: 214 TGQEINDGVLVACTKDTPGFHTKGCLNELVAHLK 315 G I G + T F+ GCLN HL+ Sbjct: 1155 LGLLIRHGNSLISTSGGKNFNLSGCLNLFKRHLR 1188 >At2g43270.1 68415.m05378 F-box family protein contains Pfam PF00646: F-box domain; contains TIGRFAM TIGR01640 : F-box protein interaction domain Length = 387 Score = 27.5 bits (58), Expect = 4.5 Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 2/27 (7%) Frame = +1 Query: 184 HNLTIPNTCCT--GQEINDGVLVACTK 258 HN+++P+TCCT Q +N +A TK Sbjct: 210 HNVSVPSTCCTMSAQMVNLDDRLAITK 236 >At5g40480.1 68418.m04909 expressed protein ; expression supported by MPSS Length = 1919 Score = 27.1 bits (57), Expect = 6.0 Identities = 16/57 (28%), Positives = 29/57 (50%) Frame = -3 Query: 339 SSEHYSDILEVSDQFVQAAFSMETGCVLSASDQNTIVDLLSGAASVRDGQVVRCPVF 169 S +H+ DIL V+ +F ++ + + S S + + A ++ G V+RC VF Sbjct: 59 SWDHH-DILSVTPEFNSSSHCSTSARLRSISPYSGRKETAVYATDIQTGMVIRCKVF 114 >At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein similar to acetylornithine deacetylase (Acetylornithinase, AO; N-acetylornithinase, NAO) [Dictyostelium discoideum] SWISS-PROT:P54638 Length = 440 Score = 27.1 bits (57), Expect = 6.0 Identities = 12/25 (48%), Positives = 15/25 (60%), Gaps = 1/25 (4%) Frame = +1 Query: 244 VACTKDTPGFHTK-GCLNELVAHLK 315 VAC D+PGFH E+V H+K Sbjct: 355 VACNLDSPGFHVLCKATEEVVGHVK 379 >At1g56590.1 68414.m06508 clathrin adaptor complexes medium subunit family protein contains Pfam profile: PF00928 adaptor complexes medium subunit family Length = 415 Score = 27.1 bits (57), Expect = 6.0 Identities = 14/47 (29%), Positives = 24/47 (51%), Gaps = 2/47 (4%) Frame = -3 Query: 249 SDQNTIVDLLSGAASV--RDGQVVRCPVFGAVDTATL*VGLHDVERS 115 S VDL+ ++ RDG++V+C ++G V + G D+ S Sbjct: 177 SSNEVYVDLVEEMDAIVNRDGELVKCEIYGEVQMNSQLTGFPDLTLS 223 >At3g21550.1 68416.m02718 expressed protein contains Pfam profile PF05078: Protein of unknown function (DUF679) Length = 184 Score = 26.6 bits (56), Expect = 7.9 Identities = 25/68 (36%), Positives = 32/68 (47%), Gaps = 3/68 (4%) Frame = -3 Query: 345 PDSS--EHYSDILEVSDQFVQAAFSMETGCVLSASDQNTIVDLLSGAASV-RDGQVVRCP 175 PDSS + S L V D FV A FS+ V+S D NT+ G S + +V P Sbjct: 91 PDSSSVDLSSKRLRVGD-FVHAFFSLIVFSVISLLDANTVNCFYPGFGSAGKIFLMVLPP 149 Query: 174 VFGAVDTA 151 V G + A Sbjct: 150 VIGVISGA 157 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,515,607 Number of Sequences: 28952 Number of extensions: 182461 Number of successful extensions: 540 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 529 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 540 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 752336160 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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