Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_B21
(416 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored... 50 8e-07
At1g14190.1 68414.m01679 glucose-methanol-choline (GMC) oxidored... 49 1e-06
At3g56060.1 68416.m06229 glucose-methanol-choline (GMC) oxidored... 48 2e-06
At5g51950.1 68418.m06447 glucose-methanol-choline (GMC) oxidored... 47 4e-06
At1g12570.1 68414.m01459 glucose-methanol-choline (GMC) oxidored... 46 1e-05
At5g51930.1 68418.m06442 glucose-methanol-choline (GMC) oxidored... 42 2e-04
At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative / (R... 39 0.001
At1g72970.1 68414.m08439 glucose-methanol-choline (GMC) oxidored... 39 0.002
At1g58440.1 68414.m06648 squalene monooxygenase, putative / squa... 31 0.31
At5g39940.1 68418.m04843 expressed protein 30 0.72
At3g57440.1 68416.m06395 hypothetical protein 29 1.3
At3g44190.1 68416.m04738 pyridine nucleotide-disulphide oxidored... 29 1.7
At5g22140.2 68418.m02577 pyridine nucleotide-disulphide oxidored... 28 2.2
At5g22140.1 68418.m02578 pyridine nucleotide-disulphide oxidored... 28 2.2
At3g09940.1 68416.m01190 monodehydroascorbate reductase, putativ... 28 2.2
At5g24160.1 68418.m02842 squalene monooxygenase 1,2 / squalene e... 28 2.9
At5g24155.1 68418.m02841 squalene monooxygenase, putative / squa... 28 2.9
At5g24150.1 68418.m02839 squalene monooxygenase 1,1 / squalene e... 28 2.9
At4g09280.1 68417.m01536 Ulp1 protease family protein contains P... 28 2.9
At3g66652.1 68416.m00776 fip1 motif-containing protein contains ... 27 6.7
At2g36760.1 68415.m04509 UDP-glucoronosyl/UDP-glucosyl transfera... 27 6.7
At2g22650.1 68415.m02684 FAD-dependent oxidoreductase family pro... 27 6.7
At2g22830.1 68415.m02711 squalene monooxygenase, putative / squa... 26 8.9
>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 503
Score = 49.6 bits (113), Expect = 8e-07
Identities = 43/126 (34%), Positives = 60/126 (47%), Gaps = 8/126 (6%)
Frame = +2
Query: 2 YVIIGGGTAGSVLAARLSEEKPKAMVLVIE-AGKP--EMLLSDVPALAHNLQLTDYAWPY 172
Y+++GGGTAG LAA LSE K VLVIE G P + L+ D ++L TD Y
Sbjct: 39 YIVVGGGTAGCSLAATLSE---KYSVLVIERGGSPFGDPLVEDKKYYGYSLINTD---EY 92
Query: 173 TMEHQPGVCLGSDEQRCYWPRGKALGGSSVTNFMLYTRG-----RPQDWNKIAADGNYGW 337
+ Q + + RG+ LGGSS N Y+R + W+K +Y W
Sbjct: 93 SSVAQSFTSVDGIKNH----RGRVLGGSSAINGGFYSRASDEFVKKAGWDKDLVQESYKW 148
Query: 338 SHDEVL 355
+V+
Sbjct: 149 VESKVV 154
>At1g14190.1 68414.m01679 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 501
Score = 48.8 bits (111), Expect = 1e-06
Identities = 43/126 (34%), Positives = 60/126 (47%), Gaps = 8/126 (6%)
Frame = +2
Query: 2 YVIIGGGTAGSVLAARLSEEKPKAMVLVIE-AGKP--EMLLSDVPALAHNLQLTDYAWPY 172
Y+++GGGTAG LAA LSE K VLVIE G P + L+ + ++L TD Y
Sbjct: 18 YIVVGGGTAGCSLAATLSE---KYSVLVIERGGSPFGDPLVEERKYFGYSLLNTD---EY 71
Query: 173 TMEHQPGVCLGSDEQRCYWPRGKALGGSSVTNFMLYTRG-----RPQDWNKIAADGNYGW 337
+ Q + E RG+ LGGSS N Y+R + W+K +Y W
Sbjct: 72 SSVAQSFTSVDGIENY----RGRVLGGSSAINGGFYSRASDEFVKKAGWDKGLVQESYKW 127
Query: 338 SHDEVL 355
+V+
Sbjct: 128 VESKVV 133
>At3g56060.1 68416.m06229 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 577
Score = 48.4 bits (110), Expect = 2e-06
Identities = 44/125 (35%), Positives = 60/125 (48%), Gaps = 4/125 (3%)
Frame = +2
Query: 2 YVIIGGGTAGSVLAARLSEEKPKAMVLVIE-AGKP--EMLLSDVPALAHN-LQLTDYAWP 169
Y+IIGGGTAG LAA LS+ A VLV+E G P + +D+ A+ L +T +W
Sbjct: 48 YIIIGGGTAGCALAATLSQ---NATVLVLERGGSPYDDPAATDIGNFANTLLNITPNSWS 104
Query: 170 YTMEHQPGVCLGSDEQRCYWPRGKALGGSSVTNFMLYTRGRPQDWNKIAADGNYGWSHDE 349
+ GV + R + LGG +V N Y+R +D+ A GW DE
Sbjct: 105 QLFISEDGV---------FNSRARVLGGGTVINAGFYSRAE-EDFVAEA-----GWERDE 149
Query: 350 VLKYY 364
V Y
Sbjct: 150 VEAAY 154
>At5g51950.1 68418.m06447 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 586
Score = 47.2 bits (107), Expect = 4e-06
Identities = 42/126 (33%), Positives = 55/126 (43%), Gaps = 9/126 (7%)
Frame = +2
Query: 2 YVIIGGGTAGSVLAARLSEEKPKAMVLVIE-AGKP--EMLLSDVPALAHNLQLTD-YAWP 169
Y+IIGGGT+G LAA LS+ A VLV+E G P +D+ A L T +W
Sbjct: 48 YIIIGGGTSGCALAATLSQ---NASVLVLERGGAPYDNPTATDIENFATTLSNTSPKSWS 104
Query: 170 YTMEHQPGVCLGSDEQRCYWPRGKALGGSSVTNFMLYTRG-----RPQDWNKIAADGNYG 334
+ GV Y R + LGG SV N YTR + +W + Y
Sbjct: 105 QLFISEDGV---------YNTRARVLGGGSVLNAGFYTRAGDEYVKETEWKTDEVEAAYE 155
Query: 335 WSHDEV 352
W +V
Sbjct: 156 WVEKKV 161
>At1g12570.1 68414.m01459 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 572
Score = 45.6 bits (103), Expect = 1e-05
Identities = 42/123 (34%), Positives = 55/123 (44%), Gaps = 6/123 (4%)
Frame = +2
Query: 2 YVIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEMLLSDVPAL-AHNLQLTDYAWPYTM 178
Y+IIGGGTAG LAA LS+ A VL++E G ++ L A L+D +
Sbjct: 48 YIIIGGGTAGCPLAATLSQ---NASVLLLERGDSPYNNPNITRLSAFGAALSDLS----- 99
Query: 179 EHQPGVCLGSDEQRCYWPRGKALGGSSVTNFMLYTRG-----RPQDWNKIAADGNYGWSH 343
E P S E R + LGG S N YTR R W+ A+ +Y W
Sbjct: 100 ESSPSQRFVS-EDGVINARARVLGGGSALNAGFYTRAGTKYVRNMGWDGALANESYQWVE 158
Query: 344 DEV 352
+V
Sbjct: 159 AKV 161
>At5g51930.1 68418.m06442 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 582
Score = 41.5 bits (93), Expect = 2e-04
Identities = 36/117 (30%), Positives = 48/117 (41%), Gaps = 5/117 (4%)
Frame = +2
Query: 2 YVIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEMLLSDVPALAHNLQLTDYAWPYTME 181
Y+IIGGGTAG LAA LS+ A VLV+E G + +++ P +
Sbjct: 75 YIIIGGGTAGCALAATLSQ---NASVLVLERGGSPYENPTATDMGNSVNTLLNNTPNSWS 131
Query: 182 HQPGVCLGSDEQRCYWPRGKALGGSSVTNFMLYTRG-----RPQDWNKIAADGNYGW 337
L E Y R + LGG SV N Y+R +W + Y W
Sbjct: 132 Q-----LFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEEAEWEMEEVEAAYEW 183
>At1g73050.1 68414.m08447 (R)-mandelonitrile lyase, putative /
(R)-oxynitrilase, putative similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|52707];
contains Pfam protile PF00732 GMC oxidoreductase
Length = 552
Score = 39.1 bits (87), Expect = 0.001
Identities = 35/99 (35%), Positives = 48/99 (48%)
Frame = +2
Query: 2 YVIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEMLLSDVPALAHNLQLTDYAWPYTME 181
Y+I+GGGTAG LAA LS+ VL++E G +V ++H+ LT +
Sbjct: 56 YIIVGGGTAGCPLAATLSQ---SFRVLLLERGGVPYNRPNV--MSHDGFLTTLTDVNNFD 110
Query: 182 HQPGVCLGSDEQRCYWPRGKALGGSSVTNFMLYTRGRPQ 298
P S E+ RG+ LGGSS N Y+R Q
Sbjct: 111 -SPAQSFIS-EEGVPNARGRVLGGSSAINAGFYSRADKQ 147
>At1g72970.1 68414.m08439 glucose-methanol-choline (GMC)
oxidoreductase family protein similar to mandelonitrile
lyase from Prunus serotina [SP|P52706, SP|P52707];
contains Pfam profile PF00732 GMC oxidoreductase
Length = 594
Score = 38.7 bits (86), Expect = 0.002
Identities = 36/124 (29%), Positives = 56/124 (45%), Gaps = 6/124 (4%)
Frame = +2
Query: 2 YVIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEMLLSDVPALAH-NLQLTDYAWPYTM 178
Y++IGGGTAG LAA LS+ VLV+E G ++V L + ++ L D +
Sbjct: 65 YIVIGGGTAGCPLAATLSQ---NFSVLVLERGGVPFTNANVSFLRNFHIGLADISASSAS 121
Query: 179 EHQPGVCLGSDEQRCYWPRGKALGGSSVTNFMLYTRG-----RPQDWNKIAADGNYGWSH 343
+ + +D Y R + LGG S N Y+R + W+ +Y W
Sbjct: 122 Q----AFVSTD--GVYNARARVLGGGSCINAGFYSRADAAFVKRAGWDPKLVKESYPWVE 175
Query: 344 DEVL 355
E++
Sbjct: 176 REIV 179
>At1g58440.1 68414.m06648 squalene monooxygenase, putative /
squalene epoxidase, putative similar to SP|O65404 (SE
1,1), SP|O65402 (SE 1,2) 6566341 dbj AB008021.1 AB008021
Length = 531
Score = 31.1 bits (67), Expect = 0.31
Identities = 11/40 (27%), Positives = 25/40 (62%)
Frame = +2
Query: 5 VIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEMLLSDV 124
+++G G AGS LA L ++K + V+ + +P+ ++ ++
Sbjct: 64 IVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGEL 103
>At5g39940.1 68418.m04843 expressed protein
Length = 480
Score = 29.9 bits (64), Expect = 0.72
Identities = 15/31 (48%), Positives = 17/31 (54%)
Frame = +2
Query: 5 VIIGGGTAGSVLAARLSEEKPKAMVLVIEAG 97
V++GGG AG A R P VLVIE G
Sbjct: 48 VVVGGGAAGVYGAIRAKTLSPDLRVLVIEKG 78
>At3g57440.1 68416.m06395 hypothetical protein
Length = 240
Score = 29.1 bits (62), Expect = 1.3
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +2
Query: 251 GSSVTNFMLYTRGRPQDWNKIAADGNYGWSHDEVLKYYMKSERSELRKYKNAPYR 415
GSS + ML + G + +++DG++G S D K + L +K A R
Sbjct: 47 GSSNLSLMLGSSGSRSCGSALSSDGSFGMSEDREFKINYTGDPFRLMNFKTARKR 101
>At3g44190.1 68416.m04738 pyridine nucleotide-disulphide
oxidoreductase family protein low similarity to
dihydrolipoamide dehydrogenase from Clostridium magnum
[GI:472330]; contains Pfam profile PF00070 Pyridine
nucleotide-disulphide oxidoreductase domain
Length = 367
Score = 28.7 bits (61), Expect = 1.7
Identities = 13/36 (36%), Positives = 23/36 (63%)
Frame = +2
Query: 5 VIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEML 112
+I+GGG +G LAA ++ + P+ V ++ G P +L
Sbjct: 138 LIVGGGPSGVELAAEIAVDFPEKKVTIVHNG-PRLL 172
>At5g22140.2 68418.m02577 pyridine nucleotide-disulphide
oxidoreductase family protein contains Pfam profile
PF00070 Pyridine nucleotide-disulphide oxidoreductase
domain
Length = 311
Score = 28.3 bits (60), Expect = 2.2
Identities = 13/36 (36%), Positives = 23/36 (63%)
Frame = +2
Query: 5 VIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEML 112
+I+GGG +G LAA ++ + P+ V ++ G P +L
Sbjct: 84 LIVGGGPSGVELAAEIAVDFPEKKVTLVHKG-PRLL 118
>At5g22140.1 68418.m02578 pyridine nucleotide-disulphide
oxidoreductase family protein contains Pfam profile
PF00070 Pyridine nucleotide-disulphide oxidoreductase
domain
Length = 365
Score = 28.3 bits (60), Expect = 2.2
Identities = 13/36 (36%), Positives = 23/36 (63%)
Frame = +2
Query: 5 VIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEML 112
+I+GGG +G LAA ++ + P+ V ++ G P +L
Sbjct: 138 LIVGGGPSGVELAAEIAVDFPEKKVTLVHKG-PRLL 172
>At3g09940.1 68416.m01190 monodehydroascorbate reductase, putative
similar to monodehydroascorbate reductase (NADH)
GB:JU0182 (Cucumis sativus)
Length = 441
Score = 28.3 bits (60), Expect = 2.2
Identities = 15/34 (44%), Positives = 18/34 (52%)
Frame = +2
Query: 2 YVIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKP 103
YVIIGGG AG A S + K L I + +P
Sbjct: 9 YVIIGGGVAGGYAAREFSNQGLKPGELAIISKEP 42
>At5g24160.1 68418.m02842 squalene monooxygenase 1,2 / squalene
epoxidase 1,2 (SQP1,2) identical to SP|O65402
Length = 517
Score = 27.9 bits (59), Expect = 2.9
Identities = 11/39 (28%), Positives = 23/39 (58%)
Frame = +2
Query: 5 VIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEMLLSD 121
+I+G G GS LA L+++ + V+ + +PE ++ +
Sbjct: 49 IIVGAGVGGSALAYALAKDGRRVHVIERDMREPERMMGE 87
>At5g24155.1 68418.m02841 squalene monooxygenase, putative /
squalene epoxidase, putative similar to SP|O65404 (SE
1,1), SP|O65402 (SE 1,2)
Length = 121
Score = 27.9 bits (59), Expect = 2.9
Identities = 10/39 (25%), Positives = 23/39 (58%)
Frame = +2
Query: 5 VIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEMLLSD 121
+I+G G GS LA L+++ + + + + +PE ++ +
Sbjct: 51 IIVGAGVGGSALAYSLAKDGRRVLAIERDMREPERMMGE 89
>At5g24150.1 68418.m02839 squalene monooxygenase 1,1 / squalene
epoxidase 1,1 (SQP1,1) identical to SP|O65404
Length = 516
Score = 27.9 bits (59), Expect = 2.9
Identities = 11/39 (28%), Positives = 23/39 (58%)
Frame = +2
Query: 5 VIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEMLLSD 121
+I+G G GS LA L+++ + V+ + +PE ++ +
Sbjct: 49 IIVGAGVGGSALAYALAKDGRRVHVIERDLREPERIMGE 87
>At4g09280.1 68417.m01536 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain
Length = 298
Score = 27.9 bits (59), Expect = 2.9
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = +2
Query: 308 KIAADGNYGWSHDEVLKYYMK 370
K+ D NYGW DE + YMK
Sbjct: 98 KLWPDSNYGWLGDEHIAAYMK 118
>At3g66652.1 68416.m00776 fip1 motif-containing protein contains
Pfam profile PF05182: Fip1 motif
Length = 980
Score = 26.6 bits (56), Expect = 6.7
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = +2
Query: 320 DGNYGWSHDEVLKYYMKSERSELRKYKN 403
D YGW H E KY ERS +R Y+N
Sbjct: 553 DDRYGWHHRE-RKYV--HERSPIRAYEN 577
>At2g36760.1 68415.m04509 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 496
Score = 26.6 bits (56), Expect = 6.7
Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
Frame = +2
Query: 2 YVIIGGGTAGSVLAARLS---EEKPKAMVLVIEAGKPEMLLSDVPALAHNLQLTDYAWPY 172
+VI GGG + L EE+ K L+I+ P+ML+ PA+ LT W
Sbjct: 322 WVIRGGGKYHELAEWILESGFEERTKERSLLIKGWSPQMLILSHPAVGG--FLTHCGWNS 379
Query: 173 TME 181
T+E
Sbjct: 380 TLE 382
>At2g22650.1 68415.m02684 FAD-dependent oxidoreductase family
protein
Length = 420
Score = 26.6 bits (56), Expect = 6.7
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +2
Query: 2 YVIIGGGTAGSVLAARLSEEKPKAMVLVIE 91
Y I+G G AG +A L +E PK + L ++
Sbjct: 24 YAILGAGFAGISVAWHLLKESPKELRLSVD 53
>At2g22830.1 68415.m02711 squalene monooxygenase, putative /
squalene epoxidase, putative similar to SP|O65404 (SE
1,1), SP|O65402 (SE 1,2), SP|O65403 (SE 2)
Length = 585
Score = 26.2 bits (55), Expect = 8.9
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +2
Query: 5 VIIGGGTAGSVLAARLSEEKPKAMVLVIEAGKPEMLLSDV 124
+I+G G AGS LA L +E + V+ + + + ++ ++
Sbjct: 126 IIVGAGVAGSALAHTLGKEGRRVHVIERDFSEQDRIVGEL 165
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,095,781
Number of Sequences: 28952
Number of extensions: 215686
Number of successful extensions: 568
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 550
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 560
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 635399168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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