Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_A24
(432 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo... 55 5e-09
SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo... 46 2e-06
SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces pombe... 31 0.100
SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 30 0.17
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce... 27 1.2
SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe... 27 1.2
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c... 27 1.6
SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosacc... 26 2.2
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 26 2.8
SPAC23H4.06 |gln1||glutamate-ammonia ligase Gln1|Schizosaccharom... 25 3.8
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 25 6.6
SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein L20|Sc... 24 8.7
SPCC16A11.15c |||sequence orphan|Schizosaccharomyces pombe|chr 3... 24 8.7
SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosom... 24 8.7
SPAC6F12.14 |cut23|apc8|anaphase-promoting complex subunit Apc8 ... 24 8.7
>SPCC191.09c |gst1||glutathione S-transferase
Gst1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 229
Score = 54.8 bits (126), Expect = 5e-09
Identities = 32/89 (35%), Positives = 48/89 (53%), Gaps = 3/89 (3%)
Frame = +2
Query: 143 AARAVGVPIEVEIVNLAKKEQLKENFVKLNPQHCVPTLKE--DDGFVLWESRAIACYLAE 316
A + + + E VN +K EQ + LNP VPTL + ++ + +WES AI YLA+
Sbjct: 21 ALKELDLTYETRYVNFSKNEQKSPEHLALNPNGRVPTLIDHHNNDYTIWESDAILIYLAD 80
Query: 317 KYGTDDQF-YPKDLKRRALVNQRLYFDSS 400
KY T+ + P+D V Q L+F +S
Sbjct: 81 KYDTERKISLPRDHPEYYKVIQYLFFQAS 109
>SPCC965.07c |gst2||glutathione S-transferase
Gst2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 230
Score = 46.4 bits (105), Expect = 2e-06
Identities = 31/86 (36%), Positives = 45/86 (52%), Gaps = 3/86 (3%)
Frame = +2
Query: 83 MAPAKLYHFPVSGPSRG-ALLAARAVGVPIEVEIVNLAKKEQLKENFVKLNPQHCVPTLK 259
MA LY GP+ +LA + + + E + K EQ + + LNP VPTL
Sbjct: 1 MAHFTLYSH-AGGPNPWKVVLALKELNLSYEQIFYDFQKGEQKCKEHLALNPNGRVPTLV 59
Query: 260 E--DDGFVLWESRAIACYLAEKYGTD 331
+ ++ + +WES AI YLA+KY TD
Sbjct: 60 DHKNNDYTIWESDAILIYLADKYDTD 85
>SPBC359.05 |abc3||ABC transporter Abc3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1465
Score = 30.7 bits (66), Expect = 0.100
Identities = 20/57 (35%), Positives = 32/57 (56%)
Frame = +2
Query: 35 YLFISSDYITLPFRLAMAPAKLYHFPVSGPSRGALLAARAVGVPIEVEIVNLAKKEQ 205
YLFI ++ PF++A+A + LYH + G S +AA + P + + N+ KK Q
Sbjct: 369 YLFI---IVSGPFQIALALSNLYH--LMGYSAFTGVAASVILFPCNIIVANVYKKFQ 420
>SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1379
Score = 29.9 bits (64), Expect = 0.17
Identities = 18/51 (35%), Positives = 25/51 (49%)
Frame = -1
Query: 318 FSAK*QAIARLSHKTNPSSSFKVGTQCCGFNFTKFSFNCSFFAKFTISTSI 166
FS K AIA HK P SS + + F+ +K +FN F+ S S+
Sbjct: 163 FSLKNAAIATSYHKFKPLSSNSLSSSGMHFSASKQAFNSPLLQTFSPSPSV 213
>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 242
Score = 27.1 bits (57), Expect = 1.2
Identities = 21/82 (25%), Positives = 37/82 (45%)
Frame = +2
Query: 161 VPIEVEIVNLAKKEQLKENFVKLNPQHCVPTLKEDDGFVLWESRAIACYLAEKYGTDDQF 340
VP E+++ + + + KL+P P + DDG ES AI +L KYG +
Sbjct: 24 VPYEIKVYDRVDG-RAPPAYTKLSPLGKSPIVV-DDGVTYIESAAILEHLVRKYGPSFKP 81
Query: 341 YPKDLKRRALVNQRLYFDSSAL 406
+D+ ++F ++L
Sbjct: 82 SEEDVAELEKYELWMHFSEASL 103
>SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 371
Score = 27.1 bits (57), Expect = 1.2
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -1
Query: 195 FAKFTISTSIGTPTALAASKAPREGPLTGKW 103
F++F S + TA +A P +GP++G W
Sbjct: 189 FSRFGTIISENSLTASSAGFTPSKGPISGNW 219
>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1111
Score = 26.6 bits (56), Expect = 1.6
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +1
Query: 52 RLHYVTVSISNGTRKTLPFSSEWTLTRSF 138
RL+ + + NG KTL + S+W RS+
Sbjct: 573 RLNTFSPEVRNGFLKTLDWLSQWACARSY 601
>SPAC6G9.04 |mug79||meiotically upregulated gene
Mug79|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 26.2 bits (55), Expect = 2.2
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +1
Query: 256 KGRRWICFMGKSCYCLLLSGKVWYRRS 336
K RW+ K YC++ SGK+ + +S
Sbjct: 1059 KNNRWVAGYWKKKYCIVDSGKLIFYKS 1085
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 25.8 bits (54), Expect = 2.8
Identities = 20/65 (30%), Positives = 28/65 (43%)
Frame = +2
Query: 119 GPSRGALLAARAVGVPIEVEIVNLAKKEQLKENFVKLNPQHCVPTLKEDDGFVLWESRAI 298
G R + A +EVE V++ + + P +P DGF L E+ AI
Sbjct: 11 GSPRVLFCVSVAAVAGVEVEHVDV-QPHNFPADLAAKFPLQKMPVFVGKDGFPLSETLAI 69
Query: 299 ACYLA 313
A YLA
Sbjct: 70 AFYLA 74
>SPAC23H4.06 |gln1||glutamate-ammonia ligase
Gln1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 359
Score = 25.4 bits (53), Expect = 3.8
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +2
Query: 269 GFVLWESRAIACYLAEKYGTDDQFYPKDL 355
G LW SR + +AE +G F+PK +
Sbjct: 216 GDQLWMSRFLLHRIAEDFGVKISFHPKPI 244
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 24.6 bits (51), Expect = 6.6
Identities = 18/69 (26%), Positives = 28/69 (40%)
Frame = +2
Query: 59 ITLPFRLAMAPAKLYHFPVSGPSRGALLAARAVGVPIEVEIVNLAKKEQLKENFVKLNPQ 238
IT+ + ++ PAKL +GP P+ E V+ KE L E + + +
Sbjct: 151 ITICYSISSEPAKLIFLETAGPIPEEDFFRVLETAPLHAEEVSKKMKELLFETYNESDGH 210
Query: 239 HCVPTLKED 265
KED
Sbjct: 211 ENEKNPKED 219
>SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein
L20|Schizosaccharomyces pombe|chr 1|||Manual
Length = 176
Score = 24.2 bits (50), Expect = 8.7
Identities = 13/37 (35%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = +2
Query: 173 VEIVNLAKKEQLKENFVK--LNPQHCVPTLKEDDGFV 277
+++V + KKE ++ N+VK LNP P G V
Sbjct: 123 LKVVEVEKKEDVRRNYVKQLLNPHLKFPLPHRRTGVV 159
>SPCC16A11.15c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 117
Score = 24.2 bits (50), Expect = 8.7
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -1
Query: 177 STSIGTPTALAASKAPREGPLTGKW*SFAGAIANRN 70
+ +G PTA A P + P+ FA ++ +RN
Sbjct: 13 TVKLGLPTARKACSLPIQNPVVAANRFFASSVHSRN 48
>SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosomal
protein L20a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 176
Score = 24.2 bits (50), Expect = 8.7
Identities = 13/37 (35%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = +2
Query: 173 VEIVNLAKKEQLKENFVK--LNPQHCVPTLKEDDGFV 277
+++V + KKE ++ N+VK LNP P G V
Sbjct: 123 LKVVEVEKKEDVRRNYVKQLLNPHLKFPLPHRRTGVV 159
>SPAC6F12.14 |cut23|apc8|anaphase-promoting complex subunit Apc8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 565
Score = 24.2 bits (50), Expect = 8.7
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +1
Query: 286 KSCYCLLLSGKVWYRRSILSKRFETSRSC*SKTIF 390
K Y L LSG V+ +R SK + +SC K F
Sbjct: 171 KDPYLLYLSGVVYRKRKQDSKAIDFLKSCVLKAPF 205
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,644,716
Number of Sequences: 5004
Number of extensions: 30935
Number of successful extensions: 104
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 102
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 154067960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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