Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I09A02NGRL0001_A08
(574 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g25240.1 68415.m03020 serpin, putative / serine protease inhi... 78 4e-15
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 75 4e-14
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 70 1e-12
At2g35580.1 68415.m04357 serpin family protein / serine protease... 67 9e-12
At1g64030.1 68414.m07252 serpin family protein / serine protease... 66 2e-11
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi... 64 5e-11
At1g62170.1 68414.m07013 serpin family protein / serine protease... 54 5e-08
At2g14540.1 68415.m01628 serpin family protein / serine protease... 48 4e-06
At1g63280.1 68414.m07154 serpin-related / serine protease inhibi... 42 3e-04
At1g51330.1 68414.m05772 serpin-related / serine protease inhibi... 40 0.002
At1g64010.1 68414.m07250 serpin, putative / serine protease inhi... 36 0.019
At5g19600.1 68418.m02333 sulfate transporter, putative similar t... 31 0.72
At4g00510.1 68417.m00070 cyclic nucleotide-binding domain-contai... 29 2.2
At3g11910.1 68416.m01460 ubiquitin-specific protease, putative s... 29 2.2
At5g50780.1 68418.m06291 ATP-binding region, ATPase-like domain-... 28 5.1
At2g19710.1 68415.m02303 expressed protein contains Pfam profi... 27 8.9
>At2g25240.1 68415.m03020 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 324
Score = 78.2 bits (184), Expect = 4e-15
Identities = 53/180 (29%), Positives = 89/180 (49%), Gaps = 6/180 (3%)
Frame = +3
Query: 3 DF-SDTKKAADIINQWANEKTRGHIKSPISDDTIDP--NAAAALFNVIFFQGHWHVPFNA 173
DF S + D +N WA T G IK +S D+ID ++ L N ++F+G W F+A
Sbjct: 61 DFASKPSEVIDEVNTWAEVHTNGLIKQILSRDSIDTIRSSTLVLANAVYFKGAWSSKFDA 120
Query: 174 SETKEKDFHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKES--GFRMLVLLPDK 347
+ TK+ DFH+ T VK P M+ + + +++ LPY E F M + LP+
Sbjct: 121 NMTKKNDFHLLDGTSVKVPFMTNYEDQYLRSYDGF--KVLRLPYIEDQRQFSMYIYLPND 178
Query: 348 LDGLSSVLDKAG-EKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKFGVSKIFN 524
+GL+ +L+K G E ++ L + +PKF+ + + ++ G++ FN
Sbjct: 179 KEGLAPLLEKIGSEPSFFDNHIPLHCISVGA-FRIPKFKFSFEFNASEVLKDMGLTSPFN 237
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 74.5 bits (175), Expect = 4e-14
Identities = 53/174 (30%), Positives = 86/174 (49%), Gaps = 7/174 (4%)
Frame = +3
Query: 21 KAADIINQ---WANEKTRGHIKSPISDDTIDP--NAAAALFNVIFFQGHWHVPFNASETK 185
K A++IN+ WA T G IK +SDD+I + L N ++F+G W F+A TK
Sbjct: 130 KPAEVINEVNAWAEVHTNGLIKEILSDDSIKTIRESMLILANAVYFKGAWSKKFDAKLTK 189
Query: 186 EKDFHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKES--GFRMLVLLPDKLDGL 359
DFH+ T+VK P M+ + Y E + +++ LPY E F M + LP+ DGL
Sbjct: 190 SYDFHLLDGTMVKVPFMTNYKKQ-YLEYYD-GFKVLRLPYVEDQRQFAMYIYLPNDRDGL 247
Query: 360 SSVLDKAGEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKFGVSKIF 521
++L++ K D R ++PKF+ + ++ + G++ F
Sbjct: 248 PTLLEEISSKPRFLDNHIPRQRILTEAFKIPKFKFSFEFKASDVLKEMGLTLPF 301
>At2g26390.1 68415.m03167 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 389
Score = 69.7 bits (163), Expect = 1e-12
Identities = 51/172 (29%), Positives = 79/172 (45%), Gaps = 8/172 (4%)
Frame = +3
Query: 30 DIINQWANEKTRGHIKSPISDDTIDP-----NAAAALFNVIFFQGHWHVPFNASETKEKD 194
D +N WA+ T G IK +S D D N+ L N ++F+ W F+A TK+ D
Sbjct: 133 DEVNIWADVHTNGLIKQILSRDCTDTIKEIRNSTLILANAVYFKAAWSRKFDAKLTKDND 192
Query: 195 FHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKES--GFRMLVLLPDKLDGLSSV 368
FH+ VK P M + + Q++ LPY E F M + LP+ DGL+++
Sbjct: 193 FHLLDGNTVKVPFMMSYKDQYLRGYDGF--QVLRLPYVEDKRHFSMYIYLPNDKDGLAAL 250
Query: 369 LDK-AGEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKFGVSKIF 521
L+K + E G L+ L T L +PK + ++ G++ F
Sbjct: 251 LEKISTEPGFLDSHIPLHRTPVDA-LRIPKLNFSFEFKASEVLKDMGLTSPF 301
>At2g35580.1 68415.m04357 serpin family protein / serine protease
inhibitor family protein similar to protein zx [Hordeum
vulgare subsp. vulgare] GI:19071, serpin [Triticum
aestivum] GI:1885350; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 374
Score = 66.9 bits (156), Expect = 9e-12
Identities = 47/164 (28%), Positives = 76/164 (46%), Gaps = 6/164 (3%)
Frame = +3
Query: 36 INQWANEKTRGHIKSPISDDTIDPNAAAALF-NVIFFQGHWHVPFNASETKEKDFHVDST 212
+N W ++T G I + + + +F N +FF G W F+ S TK+ DFH+
Sbjct: 138 VNSWVEKQTNGLITNLLPSNPKSAPLTDHIFANALFFNGRWDSQFDPSLTKDSDFHLLDG 197
Query: 213 TIVKKPTM---SLLQPLFYTENKELEAQLVELPYKESGFRMLVLLPDKLDGLSSVLDK-A 380
T V+ P M S Y K + Q F M + LPD+ DGL S+L++ A
Sbjct: 198 TKVRVPFMTGASCRYTHVYEGFKVINLQYRRGREDSRSFSMQIYLPDEKDGLPSMLERLA 257
Query: 381 GEKGLLEDVFRLRPTNRKVK-LEMPKFEIKSKLDLKPLMPKFGV 509
+G L+D L + +K L++P+F+ + + FG+
Sbjct: 258 STRGFLKDNEVLPSHSAVIKELKIPRFKFDFAFEASEALKGFGL 301
>At1g64030.1 68414.m07252 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311, serpin [Triticum
aestivum] GI:871551; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 385
Score = 65.7 bits (153), Expect = 2e-11
Identities = 49/179 (27%), Positives = 80/179 (44%), Gaps = 10/179 (5%)
Frame = +3
Query: 3 DF-SDTKKAADIINQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASE 179
DF S+ ++ +N W T IK + D ++ N + F+G W PF
Sbjct: 127 DFRSEAEEVRKEVNSWVEHHTNNLIKDLLPDGSVTSLTNKIYANALSFKGAWKRPFEKYY 186
Query: 180 TKEKDFHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKESG------FRMLVLLP 341
T++ DF++ + T V P MS + + +++ LPY+ F M LP
Sbjct: 187 TRDNDFYLVNGTSVSVPFMSSYENQYVRAYDGF--KVLRLPYQRGSDDTNRKFSMYFYLP 244
Query: 342 DKLDGLSSVLDK-AGEKGLLEDVFRLRPTNRK--VKLEMPKFEIKSKLDLKPLMPKFGV 509
DK DGL +L+K A G L+ PT R K +PKF+I+ + ++ + G+
Sbjct: 245 DKKDGLDDLLEKMASTPGFLDSHI---PTYRDELEKFRIPKFKIEFGFSVTSVLDRLGL 300
>At1g47710.1 68414.m05302 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 391
Score = 64.5 bits (150), Expect = 5e-11
Identities = 43/168 (25%), Positives = 79/168 (47%), Gaps = 5/168 (2%)
Frame = +3
Query: 36 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTT 215
+N WA ++T G I + + + D N ++F+G W+ F+ S T+E +FH+
Sbjct: 138 VNSWAEKETNGLITEVLPEGSADSMTKLIFANALYFKGTWNEKFDESLTQEGEFHLLDGN 197
Query: 216 IVKKPTMSLLQPLFYTENKELEAQLVELPYKES----GFRMLVLLPDKLDGLSSVLDK-A 380
V P M+ + + + +++ LPY + F M LPD +GLS +LDK
Sbjct: 198 KVTAPFMTSKKKQYVSAYDGF--KVLGLPYLQGQDKRQFSMYFYLPDANNGLSDLLDKIV 255
Query: 381 GEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKFGVSKIFN 524
G L++ R + + ++PKF+ D ++ G++ F+
Sbjct: 256 STPGFLDNHIPRRQVKVR-EFKIPKFKFSFGFDASNVLKGLGLTSPFS 302
>At1g62170.1 68414.m07013 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
GI:9937311 from [Cucurbita maxima]; contains Pfam
profile PF00079: Serpin (serine protease inhibitor)
Length = 433
Score = 54.4 bits (125), Expect = 5e-08
Identities = 45/165 (27%), Positives = 76/165 (46%), Gaps = 10/165 (6%)
Frame = +3
Query: 3 DF-SDTKKAADIINQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASE 179
DF S ++ +N WA+ T G IK + ++ + ++F+G W ++ S
Sbjct: 191 DFRSKAEEVRTEVNAWASSHTNGLIKDLLPRGSVTSLTDRVYGSALYFKGTWEEKYSKSM 250
Query: 180 TKEKDFHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKES------GFRMLVLLP 341
TK K F++ + T V P MS + + +++ LPY++ F M + LP
Sbjct: 251 TKCKPFYLLNGTSVSVPFMSSFEKQYIAAYDGF--KVLRLPYRQGRDNTNRNFAMYIYLP 308
Query: 342 DKLDGLSSVLDK-AGEKGLLEDVFRLRPTNR-KV-KLEMPKFEIK 467
DK L +L++ G L+ P R KV K +PKF+I+
Sbjct: 309 DKKGELDDLLERMTSTPGFLDS---HNPERRVKVGKFRIPKFKIE 350
>At2g14540.1 68415.m01628 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 407
Score = 48.0 bits (109), Expect = 4e-06
Identities = 31/120 (25%), Positives = 55/120 (45%), Gaps = 6/120 (5%)
Frame = +3
Query: 36 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTT 215
+N WA+ T IK + ++ N ++F+G W F+ S T++K FH+ +
Sbjct: 167 VNTWASRHTNDLIKEILPRGSVTSLTNWIYGNALYFKGAWEKAFDKSMTRDKPFHLLNGK 226
Query: 216 IVKKPTMSLLQPLFYTENKELEAQLVELPYKES------GFRMLVLLPDKLDGLSSVLDK 377
V P M + F +++ LPY++ F M + LPDK L ++L++
Sbjct: 227 SVSVPFMRSYEKQFIEAYDGF--KVLRLPYRQGRDDTNREFSMYLYLPDKKGELDNLLER 284
>At1g63280.1 68414.m07154 serpin-related / serine protease
inhibitor-related similar to protein zx [Hordeum vulgare
subsp. vulgare] GI:19071, serpin [Triticum aestivum]
GI:1885346
Length = 120
Score = 41.9 bits (94), Expect = 3e-04
Identities = 16/55 (29%), Positives = 29/55 (52%)
Frame = +3
Query: 36 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFH 200
+N+WA++ T G I + ++ N ++F+G W F+ S TK+ +FH
Sbjct: 16 LNKWASDHTNGLIIDLLPRGSVKSETVQVYGNALYFKGAWENKFDKSSTKDNEFH 70
>At1g51330.1 68414.m05772 serpin-related / serine protease
inhibitor-related similar to serpin [Hordeum vulgare
subsp. vulgare] CAA64599.1 GI:1197577
Length = 193
Score = 39.5 bits (88), Expect = 0.002
Identities = 25/96 (26%), Positives = 40/96 (41%), Gaps = 3/96 (3%)
Frame = +3
Query: 36 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDFHVDSTT 215
+N WA T G IK+ + ++ N ++F+G W F S T K FH+ +
Sbjct: 39 VNSWALRHTNGLIKNLLPPGSVTNQTIKIYGNALYFKGAWENKFGKSMTIHKPFHLVNGK 98
Query: 216 IVKKPTMSLLQPLF---YTENKELEAQLVELPYKES 314
V P M + + Y K L + YK++
Sbjct: 99 QVLVPFMKSYERKYMKAYNGFKVLRILQYRVDYKDT 134
>At1g64010.1 68414.m07250 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 185
Score = 35.9 bits (79), Expect = 0.019
Identities = 32/120 (26%), Positives = 60/120 (50%), Gaps = 7/120 (5%)
Frame = +3
Query: 180 TKEKDFHVDSTTIVKKPTMSLLQPLFYTENKELEAQLVELPYKESG-----FRMLVLLPD 344
TK++DFH+ + T V MS + Y E + ++++LP+++ F M LPD
Sbjct: 2 TKDRDFHLINGTSVSVSLMSSYKDQ-YIEAYD-GFKVLKLPFRQGNDTSRNFSMHFYLPD 59
Query: 345 KLDGLSSVLDK-AGEKGLLEDVFRLRPTNRKV-KLEMPKFEIKSKLDLKPLMPKFGVSKI 518
+ DGL ++++K A G L+ + KV + +PKF+I+ + G+ ++
Sbjct: 60 EKDGLDNLVEKMASSVGFLDS--HIPSQKVKVGEFGIPKFKIEFGFSASRAFNRLGLDEM 117
>At5g19600.1 68418.m02333 sulfate transporter, putative similar to
sulfate transporter [Arabidopsis thaliana] GI:2285885;
contains Pfam profiles PF00916: Sulfate transporter
family, PF01740: STAS domain; supporting cDNA
gi|14141683|dbj|AB061739.1|
Length = 634
Score = 30.7 bits (66), Expect = 0.72
Identities = 25/96 (26%), Positives = 45/96 (46%)
Frame = +3
Query: 234 MSLLQPLFYTENKELEAQLVELPYKESGFRMLVLLPDKLDGLSSVLDKAGEKGLLEDVFR 413
+ L P+F+ + + +++ E +LL L G+S++ D G + LLE
Sbjct: 521 LQLGSPVFFANSTYVRERILRWIRDEPEAIEFLLLD--LSGVSTI-DMTGMETLLEIQRI 577
Query: 414 LRPTNRKVKLEMPKFEIKSKLDLKPLMPKFGVSKIF 521
L N K+ + P+FE+ K+ L + K G +F
Sbjct: 578 LGSKNIKMVIINPRFEVLEKMMLSHFVEKIGKEYMF 613
>At4g00510.1 68417.m00070 cyclic nucleotide-binding
domain-containing protein contains Pfam profile: PF00027
cyclic nucleotide-binding domain
Length = 175
Score = 29.1 bits (62), Expect = 2.2
Identities = 17/49 (34%), Positives = 24/49 (48%)
Frame = +3
Query: 357 LSSVLDKAGEKGLLEDVFRLRPTNRKVKLEMPKFEIKSKLDLKPLMPKF 503
+ SV D E+ L+ED+ L P + +V P F S L +K L F
Sbjct: 118 IKSVSDSDKERCLVEDILYLEPLDVRVSPNCPSFSSISVLMIKVLTIHF 166
>At3g11910.1 68416.m01460 ubiquitin-specific protease, putative
strong similarity to ubiquitin-specific protease 12
(UBP12) [Arabidopsis thaliana] GI:11993471; contains
Pfam profiles PF00443: Ubiquitin carboxyl-terminal
hydrolase, PF00917: MATH domain
Length = 1115
Score = 29.1 bits (62), Expect = 2.2
Identities = 23/69 (33%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
Frame = +3
Query: 252 LFYTENKEL-EAQLVELPYKESGFRMLVLLPDKLDGLSSVLDKAGEKGLLEDVFRLRPTN 428
L Y +N+EL + +E P KE F M + KL V+++ EK L+D +LR T+
Sbjct: 794 LEYVQNRELVRFRTLEKP-KEDEFTMEL---SKLHTYDDVVERVAEKLGLDDPSKLRLTS 849
Query: 429 RKVKLEMPK 455
+ PK
Sbjct: 850 HNCYSQQPK 858
>At5g50780.1 68418.m06291 ATP-binding region, ATPase-like
domain-containing protein low similarity to microrchidia
[Homo sapiens] GI:5410257; contains Pfam profile
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein
Length = 823
Score = 27.9 bits (59), Expect = 5.1
Identities = 23/69 (33%), Positives = 34/69 (49%), Gaps = 2/69 (2%)
Frame = +3
Query: 273 ELEAQLVELPYKESGFRMLVLLPDKLDGLSSVLDKAGEKGLL--EDVFRLRPTNRKVKLE 446
EL + ELP +SG R L L + + L LDK E LL +D+ R R + ++ E
Sbjct: 702 ELPGKSSELPKPQSGPRTLSQLEQENNELRERLDKKEEVFLLLQKDLRRERELRKTLEAE 761
Query: 447 MPKFEIKSK 473
+ + K K
Sbjct: 762 VETLKNKLK 770
>At2g19710.1 68415.m02303 expressed protein contains Pfam profile:
PF03398 eukaryotic protein of unknown function, DUF292
Length = 937
Score = 27.1 bits (57), Expect = 8.9
Identities = 19/58 (32%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
Frame = -1
Query: 463 ISNFGISNFTFLLVGLSLNTSSRRPFSPA-LSNTDDKPSSLSGSRTNIRKPDSLYGNS 293
I +FG S+ L + NT +R F P S++D+ S + K DSLY +S
Sbjct: 722 IDDFGQSSRKDLYSKKASNTETRPSFMPPHPSSSDEDDSDMQHPGRTETKSDSLYSHS 779
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,882,374
Number of Sequences: 28952
Number of extensions: 275185
Number of successful extensions: 968
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 926
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 959
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1112061928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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