Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_T7_D20
(802 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 248 5e-67
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 244 2e-65
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 100 3e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 52 1e-07
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 32 0.11
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 29 0.77
SPCC965.04c |||mitochondrial inner membrane i-AAA protease compl... 28 1.3
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 27 3.1
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 4.1
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 5.4
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 9.5
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 248 bits (608), Expect = 5e-67
Identities = 118/184 (64%), Positives = 138/184 (75%), Gaps = 1/184 (0%)
Frame = -3
Query: 692 NLXXNPRIHXPTGHVXRQSXLPXRPTMNXFPSPRSQTHASSPP-TRW*NATPRHGKYMAC 516
NL PRIH P V + + S + T+ P + PR G+YMA
Sbjct: 262 NLVPYPRIHFPL--VTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMAT 319
Query: 515 CMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAV 336
C+LYRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV RAV
Sbjct: 320 CLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAV 379
Query: 335 CMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 156
CMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+DYEEV
Sbjct: 380 CMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEV 439
Query: 155 GMDS 144
G DS
Sbjct: 440 GQDS 443
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 244 bits (596), Expect = 2e-65
Identities = 117/184 (63%), Positives = 136/184 (73%), Gaps = 1/184 (0%)
Frame = -3
Query: 692 NLXXNPRIHXPTGHVXRQSXLPXRPTMNXFPSPRSQTHASSPP-TRW*NATPRHGKYMAC 516
NL PRIH P V + + S + T+ P + PR G+YMA
Sbjct: 258 NLVPYPRIHFPL--VTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMAT 315
Query: 515 CMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAV 336
C+LYRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI +PP + G ++AKV RAV
Sbjct: 316 CLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAV 375
Query: 335 CMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEV 156
CMLSNTT+IAEAW+RLDHKFDLMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+DYEEV
Sbjct: 376 CMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEV 435
Query: 155 GMDS 144
G DS
Sbjct: 436 GQDS 439
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 100 bits (239), Expect = 3e-22
Identities = 51/137 (37%), Positives = 79/137 (57%), Gaps = 3/137 (2%)
Frame = -3
Query: 548 ATPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVP 369
A PRHG+Y+ L+RG V K+V+ I +++TK + FV+W P + PP
Sbjct: 303 ADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPK--- 359
Query: 368 GGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAR-- 195
DL + + + N+T+I E + RL +F M+ ++AF+HWY GEGM+E EF+EA
Sbjct: 360 --DL---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESN 414
Query: 194 -EDLAALEKDYEEVGMD 147
DL + + Y+E G+D
Sbjct: 415 MNDLVSEYQQYQEAGID 431
Score = 29.1 bits (62), Expect = 0.77
Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = -2
Query: 675 PYPLXHWSRX--APIXSAEXAYHEQXSVAEITNACFEPANQMVKCDP 541
P+P H+ AP+ + + + SV E+T F+ N MV DP
Sbjct: 259 PFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADP 305
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 52.0 bits (119), Expect = 1e-07
Identities = 31/119 (26%), Positives = 61/119 (51%), Gaps = 3/119 (2%)
Frame = -3
Query: 509 LYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCM 330
+ +G+ P DV+ ++ I+ +R F+ W P +V ++ + P + ++ + M
Sbjct: 323 IIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGL-----M 377
Query: 329 LSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEE---GEFSEAREDLAALEKDYE 162
L+N T+IA + R ++D + + AF+ Y E + E EF +R+ +A L +YE
Sbjct: 378 LANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYE 436
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 31.9 bits (69), Expect = 0.11
Identities = 20/66 (30%), Positives = 26/66 (39%)
Frame = -1
Query: 565 QPDGEMRPPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTS 386
QP P + S VV P + RP++P P LS V PV+ V +
Sbjct: 531 QPPAAPVAPEVPSAPQRPAAPVVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVP 590
Query: 385 HPPWCP 368
PP P
Sbjct: 591 QPPVAP 596
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 29.1 bits (62), Expect = 0.77
Identities = 12/29 (41%), Positives = 13/29 (44%)
Frame = +2
Query: 485 WVRRHHGTAYSKPCTCHDGGSHFTIWLAG 571
W R H Y+KPC DGG W G
Sbjct: 16 WRRDHPFGFYAKPCKSSDGGLDLMNWKVG 44
>SPCC965.04c |||mitochondrial inner membrane i-AAA protease complex
subunit Yme1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 709
Score = 28.3 bits (60), Expect = 1.3
Identities = 21/66 (31%), Positives = 29/66 (43%), Gaps = 2/66 (3%)
Frame = -1
Query: 298 GLALTTSSTSCTPSVLSCTGTSVRVWRRESSPKPVRTWLPS--RRITKKSAWTPLKARVR 125
G+ TS TS T S S++ S+P P W P+ S+ TP+ V
Sbjct: 148 GVPKFTSDTSSTVSSTPSLNHSLQNSMPPSTPTPPPVWAPTIVSSALGTSSKTPVYVVVD 207
Query: 124 EPKSTK 107
EP+ TK
Sbjct: 208 EPRFTK 213
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2244
Score = 27.1 bits (57), Expect = 3.1
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -2
Query: 603 SVAEITNACFEPANQMVKCDPPSWQV 526
SV ++T ACFEP+ V P W +
Sbjct: 800 SVTKVTCACFEPSLDYVVVKIPRWDL 825
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 4.1
Identities = 14/36 (38%), Positives = 17/36 (47%), Gaps = 4/36 (11%)
Frame = -3
Query: 629 PXRPTMNXFPSPR----SQTHASSPPTRW*NATPRH 534
P RP + P P+ S HA PP + NA P H
Sbjct: 1356 PVRPAVPTSPKPQIPDSSNVHAPPPPVQPMNAMPSH 1391
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 5.4
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = -1
Query: 466 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 380
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.4 bits (53), Expect = 9.5
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = -1
Query: 469 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 374
P PS+P+++SN ST G+Q V V + W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,815,149
Number of Sequences: 5004
Number of extensions: 57203
Number of successful extensions: 208
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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