Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= FWDP03_T7_C08
         (793 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo...   110   2e-25
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo...   109   3e-25
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    28   1.3  
SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch...    28   1.3  
SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp...    25   9.4  
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||...    25   9.4  

>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  110 bits (265), Expect = 2e-25
 Identities = 62/132 (46%), Positives = 82/132 (62%), Gaps = 2/132 (1%)
 Frame = -2

Query: 666 PGVEXXXXXXXXXXXLAPGGHLGRFVIWTQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKM 487
           PGVE           LAPGGHLGRFVIWT+SAFG LD +FGS    ++ KKN+ LP+  +
Sbjct: 225 PGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVFGSTTEAAQLKKNYFLPENII 284

Query: 486 ANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNKAMLKLNPYAAVLKRKAILE 313
           +N D+TRL+ SDEI+ +++A    RV RA  +K NPL N   + +LNPYA     KA ++
Sbjct: 285 SNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLARLNPYAKAY--KANVK 342

Query: 312 LRRRKNLKALAD 277
           L   K  KA  +
Sbjct: 343 LNTGKTPKAAGE 354


>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  109 bits (263), Expect = 3e-25
 Identities = 61/132 (46%), Positives = 82/132 (62%), Gaps = 2/132 (1%)
 Frame = -2

Query: 666 PGVEXXXXXXXXXXXLAPGGHLGRFVIWTQSAFGRLDPLFGSWKTPSKQKKNFNLPQPKM 487
           PGVE           LAPGGHLGRFVIWT+SAFG LD +FGS    ++ KKN+ LP+  +
Sbjct: 225 PGVEIVNVRRLNLLQLAPGGHLGRFVIWTKSAFGLLDSVFGSTTEVAQLKKNYFLPENII 284

Query: 486 ANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNKAMLKLNPYAAVLKRKAILE 313
           +N D+TRL+ SDEI+ +++A    RV RA  +K NPL N   + +LNPYA     KA ++
Sbjct: 285 SNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLSRLNPYAKAY--KANVK 342

Query: 312 LRRRKNLKALAD 277
           +   K  KA  +
Sbjct: 343 INSEKTPKAAGE 354


>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
            Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 27/109 (24%), Positives = 46/109 (42%)
 Frame = -2

Query: 525  KQKKNFNLPQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPY 346
            KQK + +  Q K     LT+   S E + VL       +R T+K   LTN+  ++K +P 
Sbjct: 730  KQKAHLSADQCKQLANVLTQA--SPEAKTVLEQHRLSEMRKTKKQTELTNSAHVIKPSPT 787

Query: 345  AAVLKRKAILELRRRKNLKALADAEKSGLKLSKRNPAMKAEKLRERRRK 199
              +  ++   +      +  L   ++    L+KRN   K +      RK
Sbjct: 788  PQITVKQNTTKSSSAPRMGMLEQLKQE--YLTKRNFESKLKSSAVSSRK 834


>SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor
           Cwf22|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 28.3 bits (60), Expect = 1.3
 Identities = 19/90 (21%), Positives = 43/90 (47%), Gaps = 2/90 (2%)
 Frame = -2

Query: 459 KSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNPYAAVLKRKAILELRRRKNLKA-- 286
           +S  +  +    +K+ IR  R+L+P    ++  + N Y+ + +  ++   R R       
Sbjct: 659 RSRSVTPINNINHKKYIRKDRELSPRGRERSSNR-NSYSDLSRSSSLSRGRSRSYTPEGR 717

Query: 285 LADAEKSGLKLSKRNPAMKAEKLRERRRKN 196
           L ++E  G +    +PA +  + R+R R++
Sbjct: 718 LIESEDKGYRSRSSSPASRKYRSRQRYRRS 747


>SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex
           subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 500

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 24/93 (25%), Positives = 42/93 (45%), Gaps = 1/93 (1%)
 Frame = -2

Query: 477 DLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAM-LKLNPYAAVLKRKAILELRRR 301
           ++T  LK DE+RK+    N +       L  + +++   + L+    + K +  +EL   
Sbjct: 366 EVTETLKGDELRKISLQVNVKFSEEEVTLEDVDDDEIEDILLDKDEILTKTQVWMEL--- 422

Query: 300 KNLKALADAEKSGLKLSKRNPAMKAEKLRERRR 202
            N   LA+ E   LKL +        + R+RRR
Sbjct: 423 -NKDYLAEEEAKNLKLQEDLKKGIVRQPRKRRR 454


>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1158

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 17/54 (31%), Positives = 32/54 (59%)
 Frame = +1

Query: 343 RVRIEFQHRLVVSERVQFACSTDHAFVGSTEDLPDLIRLEKTCEVSVGHLRLGQ 504
           +V +EF+ RL + +RV+       AF+GS E +  L+ + +T + ++  L LG+
Sbjct: 207 QVAVEFRKRLNIGDRVKDGLLYKDAFLGS-EAVDVLMHIVRTTDRNLA-LLLGR 258


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,322,001
Number of Sequences: 5004
Number of extensions: 39944
Number of successful extensions: 141
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 385381248
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)