Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_O21
(843 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 173 2e-44
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 80 3e-16
SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces ... 30 0.47
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 28 1.4
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 2.5
SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces pom... 27 2.5
SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein Rad4|Schizosac... 26 7.7
>SPBC354.02c |sec61||translocon alpha subunit
Sec61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 173 bits (422), Expect = 2e-44
Identities = 88/178 (49%), Positives = 114/178 (64%), Gaps = 1/178 (0%)
Frame = +2
Query: 272 TLFIFLVCCQIPLFGIMSSDSADPFYWIRVILASNRGTLMELGISPIVTSGLIMQLLAGA 451
TL IFLV Q+PL+GI+SSDS+DP W+R+ILA+NRGTLMELGISPIVTS +++QLL G+
Sbjct: 39 TLLIFLVMSQVPLYGIVSSDSSDPLLWLRMILAANRGTLMELGISPIVTSSMLVQLLVGS 98
Query: 452 KIIEVG-DTPKDRALFNGAQKLFGMVITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLF 628
++IEV + DR ++ QK ++I GQA YV+TGMYG P ++GAG+CLL+I+QL
Sbjct: 99 QLIEVNMELKSDREMYQLVQKFLAIIIAFGQATAYVLTGMYGRPQDLGAGICLLLILQLA 158
Query: 629 VAGLIVLLLDEXXXXXXXXXXXXXXXXXXKHL*NHCMEGISPATXXTGRGQNSXGAVI 802
A LIVLLLDE + N + SP T G GAVI
Sbjct: 159 AASLIVLLLDELLQKGYGLGSGISLFIATINCENIFWKAFSPTTYHIANGVQFEGAVI 216
Score = 59.3 bits (137), Expect = 7e-10
Identities = 22/43 (51%), Positives = 34/43 (79%)
Frame = +1
Query: 166 IKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAIHIIYFLSM 294
++FL+++KPF LPEIA PERK+ F++K+LWT + ++ FL M
Sbjct: 4 LRFLDLVKPFAPFLPEIAAPERKVPFKQKMLWTGVTLLIFLVM 46
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 80.2 bits (189), Expect = 3e-16
Identities = 42/128 (32%), Positives = 74/128 (57%), Gaps = 1/128 (0%)
Frame = +2
Query: 281 IFLVCCQIPLFGIMSSDSADPFYWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKII 460
++ + IP++G +D+ DP RV+ S+ LM G++PI S ++Q+LA K I
Sbjct: 42 VYQILSIIPVYGAEKTDTLDPINNFRVLDGSSASGLMITGLAPIYLSSFLLQILASKKKI 101
Query: 461 EVG-DTPKDRALFNGAQKLFGMVITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAG 637
V + DR LF AQK+ ++ + A+ YV +G YG S++G +++I+Q+F+ G
Sbjct: 102 AVNFNLIIDRVLFQNAQKVVSALLYLILAVTYVSSGYYGSFSDLGIFRFIMLILQIFLPG 161
Query: 638 LIVLLLDE 661
++ + L E
Sbjct: 162 IVCIYLCE 169
Score = 38.3 bits (85), Expect = 0.001
Identities = 17/48 (35%), Positives = 29/48 (60%), Gaps = 2/48 (4%)
Frame = +1
Query: 163 GIKFLEVIKPFCSILPEIAKPERKIQFREKVLWTA--IHIIYFLSMLP 300
G +F+ IKP S+LPE+ P+ ++ EK+ W A + + LS++P
Sbjct: 3 GARFINFIKPLSSLLPEVEGPKTHLELVEKLGWMAGCVVVYQILSIIP 50
>SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 346
Score = 29.9 bits (64), Expect = 0.47
Identities = 23/67 (34%), Positives = 30/67 (44%), Gaps = 4/67 (5%)
Frame = -1
Query: 513 NFCAPLNKALSFGVSPTS-MILAPASNCMISPDVTIGEIPSSINV---PLFDARITRIQ* 346
+ CA +NKAL G+ TS A C PD I + S V PL + RI ++
Sbjct: 104 SLCAAINKALDAGIPITSPAFYADEKQC---PDTLIASVFDSATVEKIPLLEERIRIMRA 160
Query: 345 KGSALSD 325
G L D
Sbjct: 161 SGRVLVD 167
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 28.3 bits (60), Expect = 1.4
Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 3/63 (4%)
Frame = -3
Query: 694 PRTQSISFLEQ---FIEQQHNKTGHKQLYDNEEAHSGTNFTGFTIHSSHYINNSLTNSNH 524
P +S S ++ F E Q++ TG+K + A +G + G + +S + SNH
Sbjct: 1703 PSVESFSIIDNDNPFHEGQNSSTGYKDIVQGLLAENGVSEAGVDVMTSIVSSTIPIVSNH 1762
Query: 523 HTK 515
T+
Sbjct: 1763 QTE 1765
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 27.5 bits (58), Expect = 2.5
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = -3
Query: 646 HNKTGHKQLYDNEEAHSGTNFTGFTIHSSHYINNSLTNSNH 524
+NK HK+L DNE SGT + T+ + + N T H
Sbjct: 515 NNKNHHKELEDNE---SGTRISPSTLGDTSFPNGIFTLLTH 552
>SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 336
Score = 27.5 bits (58), Expect = 2.5
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = -3
Query: 640 KTGHKQLYDNEEAHSGTNFTGFTIHSSHYINN-SLTNSNHHT 518
+T +Y + E H+G G +HSS NN S T+SN T
Sbjct: 144 QTHGDDVYLSTEEHNGNAARGLPVHSSFKQNNSSQTSSNKGT 185
>SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein
Rad4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 648
Score = 25.8 bits (54), Expect = 7.7
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -2
Query: 215 ISGNILQNGLITSKNFIPILIYC 147
+ G L +GL+ K+F+P L C
Sbjct: 83 VQGEDLDDGLLVDKHFLPTLFKC 105
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,535,288
Number of Sequences: 5004
Number of extensions: 76431
Number of successful extensions: 208
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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