Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_O09
(847 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1006.07 |||translation initiation factor eIF4A|Schizosacchar... 28 1.4
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 27 2.5
SPBC713.05 |||WD repeat protein, human MAPK organizer 1 |Schizos... 26 5.8
SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 26 5.8
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 5.8
SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomy... 26 5.8
SPAC57A10.02 |cdr2||GIN4 family protein kinase Cdr2|Schizosaccha... 26 5.8
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 26 7.7
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 26 7.7
>SPAC1006.07 |||translation initiation factor
eIF4A|Schizosaccharomyces pombe|chr 1|||Manual
Length = 392
Score = 28.3 bits (60), Expect = 1.4
Identities = 9/21 (42%), Positives = 17/21 (80%)
Frame = +3
Query: 288 AIDLLTNDECRLLLEVEDFFN 350
+I+ +TND+ R++ E+E F+N
Sbjct: 358 SINFVTNDDVRMMREIEQFYN 378
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 27.5 bits (58), Expect = 2.5
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +1
Query: 421 STRHHRRS*NQVPPNSLTIFCSS*TVNANK 510
STR + N+VP N L I+C + NK
Sbjct: 74 STRERLKLYNKVPDNGLVIYCGEVIMEGNK 103
>SPBC713.05 |||WD repeat protein, human MAPK organizer 1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 26.2 bits (55), Expect = 5.8
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 683 LWCCRVNAFNTAAESAGTRDSVAS 754
LW CR N+F+ A +DSV+S
Sbjct: 124 LWDCRSNSFSPIQVLADAKDSVSS 147
>SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 396
Score = 26.2 bits (55), Expect = 5.8
Identities = 24/85 (28%), Positives = 36/85 (42%), Gaps = 2/85 (2%)
Frame = +2
Query: 50 FSCNFAITMGF*YLIVCTPACAFTHN-IKMLAKMAPS-QDKLVHLHKTPTSLDINPSGLL 223
F C I++ F I A A H I L + P +D H K I+P LL
Sbjct: 310 FVCPSCISLDFDLQI---QAFARQHRVISTLGVVYPEREDSCFHKIKAAQWHQISPRSLL 366
Query: 224 FAFVELNHYNNECESEGVMGCGHRF 298
F F E NH +++ ++ G ++
Sbjct: 367 FQFQEQNHIHHKKIRRKLLAAGWKW 391
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 26.2 bits (55), Expect = 5.8
Identities = 16/62 (25%), Positives = 25/62 (40%)
Frame = +3
Query: 552 PSIFENISEVPERVDPQPPAAVLASSPFVTSQPTEELLREFETVYGAVELTHLTPPQSPP 731
P+ S V P P A++ AS+P + S P ++ + T + P Q P
Sbjct: 895 PAAMSRTSSVSTLPPPPPTASMTASAPAIASPPPPKVGETYHP--PTASGTRVPPVQQPS 952
Query: 732 GP 737
P
Sbjct: 953 HP 954
>SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 436
Score = 26.2 bits (55), Expect = 5.8
Identities = 26/80 (32%), Positives = 35/80 (43%), Gaps = 1/80 (1%)
Frame = +2
Query: 5 GLLIYGSQLTDVFLVFSCNFAITMGF*YLIVCTPACAFTHNIKMLAKMAPSQDKLVHL-H 181
GLL++G L+D+F F I MG V AC I A D LVH H
Sbjct: 99 GLLMFGCILSDIFEQFCQVETIVMG----DVTYGACC----IDDFTARALDCDFLVHYGH 150
Query: 182 KTPTSLDINPSGLLFAFVEL 241
+D P +L+ FV++
Sbjct: 151 SCLIPVDQTPIKVLYVFVDI 170
>SPAC57A10.02 |cdr2||GIN4 family protein kinase
Cdr2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 5.8
Identities = 19/75 (25%), Positives = 34/75 (45%), Gaps = 2/75 (2%)
Frame = +1
Query: 394 RSKSITPRVSTRH--HRRS*NQVPPNSLTIFCSS*TVNANKRIFSPTG*RKKLTSLRSSK 567
R + PRV+ + + + N + N+ ++ T+N ++R+ +P+G R L S
Sbjct: 529 RGSAFNPRVTRFNVGNEQFSNNIDNNNYNQPYANATMNNSRRLRTPSGERSMRADLSQSP 588
Query: 568 TFLKFLNAWTHNPRQ 612
LN H RQ
Sbjct: 589 ASYDSLNVPKHRRRQ 603
>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
membrane proteins, ESCRT 0 complex|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 610
Score = 25.8 bits (54), Expect = 7.7
Identities = 13/52 (25%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = +3
Query: 531 LEEKVDLPSIFENISEVPERVDPQPPAAVLASSPFVTSQPT-EELLREFETV 683
+E + D+ S N S +P+ P V+ SSP + P+ +++ + +T+
Sbjct: 470 IEPEPDVISTVRNSSTIPQASSSSVPKIVVDSSPVTENPPSHSDVMGQKDTI 521
>SPCC417.08 |tef3||translation elongation factor
eEF3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1047
Score = 25.8 bits (54), Expect = 7.7
Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
Frame = -2
Query: 705 ALTRQHHKPFRIPAAVLQWAARSQKDLK-LEPLPGVVGPRVQELQK 571
A T H P + P+ +QW +S +DL+ ++ V+ +E K
Sbjct: 746 AFTHLGHHPDKTPSEYIQWRFQSGEDLEAMDKASRVISEADEEAMK 791
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,129,002
Number of Sequences: 5004
Number of extensions: 61352
Number of successful extensions: 166
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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