Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_N06
(908 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 321 1e-88
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 318 9e-88
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 160 3e-40
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 128 8e-31
SPBC16G5.10 |||exosome subunit Rrp42 |Schizosaccharomyces pombe|... 27 4.9
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.5
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.5
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 321 bits (788), Expect = 1e-88
Identities = 141/215 (65%), Positives = 171/215 (79%)
Frame = +1
Query: 103 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 282
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 283 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 462
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 463 RIRKLADQCTCLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVXLP 642
+IR++AD C+ LQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQV
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 643 SSSPYNSILTTHTTLEHSDCAFMVENEAIYDICRR 747
PYNS+LTTH TL+ +DC FMV+NE+ YDICRR
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 318 bits (780), Expect = 9e-88
Identities = 144/220 (65%), Positives = 174/220 (79%), Gaps = 5/220 (2%)
Frame = +1
Query: 103 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 267
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 268 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 447
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 448 DLVLDRIRKLADQCTCLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 627
D VL+RIR++AD C+ LQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 628 QVXLPSSSPYNSILTTHTTLEHSDCAFMVENEAIYDICRR 747
QV PYNS+LTTH TL++SDC FMV+NEA YDICRR
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 160 bits (388), Expect = 3e-40
Identities = 79/212 (37%), Positives = 116/212 (54%)
Frame = +1
Query: 103 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 282
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 283 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 462
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 463 RIRKLADQCTCLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVXLP 642
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 643 SSSPYNSILTTHTTLEHSDCAFMVENEAIYDI 738
PYN+ L+ H +E+SD F ++NEA+ I
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSI 210
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 128 bits (310), Expect = 8e-31
Identities = 73/214 (34%), Positives = 116/214 (54%), Gaps = 3/214 (1%)
Frame = +1
Query: 106 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 285
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 286 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 459
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 460 DRIRKLADQCTCLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VX 636
D I + AD L+GF + H L+ERL+ Y KK ++++P Q V
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 637 LPSSSPYNSILTTHTTLEHSDCAFMVENEAIYDI 738
PYNS+L ++D +++N A+ I
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHI 213
>SPBC16G5.10 |||exosome subunit Rrp42 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 299
Score = 26.6 bits (56), Expect = 4.9
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +1
Query: 559 LMERLSVDYGKKSKLEFAIYPAPQVXLPSSSPYNSILT 672
L+ L+VDY K + + I V + SSSPY +IL+
Sbjct: 108 LLNALAVDYLKFTPSKAWIIHVDAVVILSSSPYENILS 145
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.5
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 182 STASSLMARCPQTRPSGVETILSTLSSARPELAST 286
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.5
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 201 WPDAHRQDHRGWRRFFQHFLQR 266
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,265,766
Number of Sequences: 5004
Number of extensions: 66250
Number of successful extensions: 224
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 460503700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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