BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_M09
(876 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 29 0.87
SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|ch... 29 1.1
SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 28 2.0
SPAC17G8.06c |||dihydroxy-acid dehydratase|Schizosaccharomyces p... 28 2.0
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 27 2.7
SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate syn... 27 3.5
SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces p... 27 4.6
SPBC36B7.08c |||nucleosome assembly protein |Schizosaccharomyces... 26 6.1
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 26 6.1
SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint compone... 26 6.1
SPBC215.07c |||PWWP domain protein|Schizosaccharomyces pombe|chr... 26 8.1
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 29.1 bits (62), Expect = 0.87
Identities = 14/46 (30%), Positives = 25/46 (54%)
Frame = +2
Query: 491 QAHMRSEEVGPEDEIPADESEYLEEDPDFGDDEPLKREEPQPTVSA 628
+++ R E+V E+E DE+ +D D +++ L+ E P SA
Sbjct: 242 ESYERDEDVDEEEEEDDDENNDEGDDEDENENDELRSENGSPNPSA 287
>SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 783
Score = 28.7 bits (61), Expect = 1.1
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = -3
Query: 319 IKAFQQHIRVNFIFIHPSVLR 257
IK+F H++ NF F H ++LR
Sbjct: 265 IKSFYGHVQANFFFFHETLLR 285
>SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 27.9 bits (59), Expect = 2.0
Identities = 9/22 (40%), Positives = 12/22 (54%)
Frame = +2
Query: 347 PNGPNRLICEVCITRLRDACNF 412
P+ + C C+ RLR CNF
Sbjct: 462 PDSTSHASCTYCVARLRSVCNF 483
>SPAC17G8.06c |||dihydroxy-acid dehydratase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 598
Score = 27.9 bits (59), Expect = 2.0
Identities = 13/30 (43%), Positives = 19/30 (63%), Gaps = 2/30 (6%)
Frame = +2
Query: 395 RDACNF--KKQVFECEKKFIDMVARGEFRK 478
++ NF K +VF+ E FI + RGEF+K
Sbjct: 435 KEGLNFTGKARVFDAENDFIAALERGEFKK 464
>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 442
Score = 27.5 bits (58), Expect = 2.7
Identities = 14/43 (32%), Positives = 20/43 (46%)
Frame = -2
Query: 272 SKCTQNLNLCSTLQKDSTGKGRDSPSPQYRQTPSLYHDSNITI 144
S N + S + S+ PS + R TP+ HDS +TI
Sbjct: 294 SPAATNSAISSPFVRKSSSDLEAKPSKKQRSTPAFSHDSPLTI 336
>SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate
synthase Lsp1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 534
Score = 27.1 bits (57), Expect = 3.5
Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 2/56 (3%)
Frame = +2
Query: 413 KKQVFECEKKFIDMVARGEFRKVLIYQAHMRSEEVGPE--DEIPADESEYLEEDPD 574
+KQVF C FID +F + +++EV + D + +DE E E+ D
Sbjct: 475 RKQVFHCFLSFIDSYNEEDFSYLSDIGITEKTQEVSSKLPDVLSSDEEEDSAENKD 530
>SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 932
Score = 26.6 bits (56), Expect = 4.6
Identities = 27/90 (30%), Positives = 39/90 (43%), Gaps = 5/90 (5%)
Frame = +2
Query: 302 LLEC---FDISISQINEGPNGPNRLICEVCITRLRDACNFKKQVFECEKKFIDMVARGEF 472
LL C F SI + P+G + +C L C F+K+ +F DMV+ E
Sbjct: 314 LLSCQNVFQKSIGDVCWSPDGLSLFLCSYDGNVL--VCTFEKE------EFGDMVSDEEI 365
Query: 473 RKVLIYQAHMRSEEVGPED--EIPADESEY 556
K L H R V PE ++ +E+ Y
Sbjct: 366 SKALAKYGHGRHGIVLPESAKQLELEETAY 395
>SPBC36B7.08c |||nucleosome assembly protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 26.2 bits (55), Expect = 6.1
Identities = 20/55 (36%), Positives = 27/55 (49%), Gaps = 3/55 (5%)
Frame = +2
Query: 455 VARGEFRKVLIYQAHMRSEEVGPEDE---IPADESEYLEEDPDFGDDEPLKREEP 610
+A F + Y +EE EDE + DE E EED + GD+E K+E P
Sbjct: 185 LAEDLFPNAVKYFTEAMTEEASDEDESVDLEEDEEEEDEEDEE-GDEE--KQEPP 236
>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 580
Score = 26.2 bits (55), Expect = 6.1
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +2
Query: 482 LIYQAHMRSEEVGPEDEIPADESEYLEEDPDFGD 583
L+ +A R + V P ++P DE + EDP+F D
Sbjct: 413 LLKEAGYRKQLV-PGQKVPEDEEFHYCEDPEFDD 445
>SPCC895.07 |alp14|mtc1|Mad2-dependent spindle checkpoint component
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 809
Score = 26.2 bits (55), Expect = 6.1
Identities = 23/74 (31%), Positives = 33/74 (44%)
Frame = -1
Query: 468 SPLATMSMNFFSHSNTCFLKLHASRSLVMQTSQISLFGPFGPSFICDILISKHSNSISA* 289
SP+ F +NT FL+L S S Q S + F P P I +++ S SA
Sbjct: 697 SPIGDRKPAFMRRANTDFLELSTSPS--FQRS-VREFEPTRPKLYSSIDVNQRSPLASAK 753
Query: 288 TSFSFIQVYSEPKS 247
T+ +F P+S
Sbjct: 754 TNGNFTFHAELPRS 767
>SPBC215.07c |||PWWP domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 568
Score = 25.8 bits (54), Expect = 8.1
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = +2
Query: 533 IPADESEYLEEDPDFGDDEPLKREEPQPTVSAEPLQIKGKR 655
+P+ ESE EE+ D EP EE A + KG +
Sbjct: 223 VPSSESEVSEEESDQEMSEPSPIEEDYNDTKARRITRKGTK 263
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,145,636
Number of Sequences: 5004
Number of extensions: 63820
Number of successful extensions: 201
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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