Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= FWDP03_FL5_M06
(889 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 267 1e-72
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 260 2e-70
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 132 6e-32
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 116 4e-27
SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyc... 27 2.7
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.2
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.2
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 267 bits (655), Expect = 1e-72
Identities = 117/177 (66%), Positives = 143/177 (80%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELHCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSGTGAGK 285
MRE IS+HVGQAG QIGNACWEL+CLEHGIQP+G M + D F+TFFS TG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQV 636
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQV
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQV 177
Score = 52.4 bits (120), Expect = 8e-08
Identities = 26/52 (50%), Positives = 31/52 (59%)
Frame = +2
Query: 686 LEHSDCAFMVDNEXIYDICHRXSRH*APNLXNLNRLXXQIVXSXXAFLKSTG 841
L+ +DC FMVDNE YDIC R P+ NLNRL Q+V S A L+ G
Sbjct: 195 LDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEG 246
Score = 25.8 bits (54), Expect = 8.2
Identities = 16/52 (30%), Positives = 18/52 (34%)
Frame = +3
Query: 621 PRASGSTAVVEPYNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDIERPTY 776
P ST+VVEPYN LDIERP+Y
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSY 224
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 260 bits (637), Expect = 2e-70
Identities = 118/182 (64%), Positives = 145/182 (79%), Gaps = 5/182 (2%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELHCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSG 270
MRE ISVHVGQAGVQIGNACWEL+CLEHGI PDG PT+ K +D F TFFS
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 271 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 450
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 451 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 630
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 631 QV 636
QV
Sbjct: 180 QV 181
Score = 55.2 bits (127), Expect = 1e-08
Identities = 27/52 (51%), Positives = 31/52 (59%)
Frame = +2
Query: 686 LEHSDCAFMVDNEXIYDICHRXSRH*APNLXNLNRLXXQIVXSXXAFLKSTG 841
L++SDC FMVDNE YDIC R P NLNRL Q+V S A L+ G
Sbjct: 199 LDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAG 250
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/52 (32%), Positives = 18/52 (34%)
Frame = +3
Query: 621 PRASGSTAVVEPYNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDIERPTY 776
P ST+VVEPYN LDIERPTY
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTY 228
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 132 bits (319), Expect = 6e-32
Identities = 65/176 (36%), Positives = 95/176 (53%)
Frame = +1
Query: 106 MRECISVHVGQAGVQIGNACWELHCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSGTGAGK 285
MRE + + GQ G Q+G A W EHG+ G T + N +F+ GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 286 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 465
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 466 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ 633
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 116 bits (279), Expect = 4e-27
Identities = 63/177 (35%), Positives = 98/177 (55%), Gaps = 2/177 (1%)
Frame = +1
Query: 109 RECISVHVGQAGVQIGNACWELHCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSGTGAGKH 288
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF + ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 289 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 462
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 463 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ 633
D I + AD L+GF + H L+ERL+ Y KK ++++P Q
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176
>SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 379
Score = 27.5 bits (58), Expect = 2.7
Identities = 16/58 (27%), Positives = 22/58 (37%)
Frame = +1
Query: 127 HVGQAGVQIGNACWELHCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSGTGAGKHVPRA 300
H G +G + ++ + DG T GG F F G GAG H R+
Sbjct: 131 HAFAGGGGMGGGMGGMGGMDDDMDMDGGFGTRTRGGGMPGGFANMFGGGGAGPHARRS 188
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.2 bits (55), Expect = 6.2
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 185 STASSLMARCPQTRPSGVETILSTLSSAGPELAST 289
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 204 WPDAHRQDHRGWRRFFQHFLQR 269
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,371,850
Number of Sequences: 5004
Number of extensions: 69920
Number of successful extensions: 223
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 218
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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